LOCUS       sequence-449282-        7441 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mamalian cell expression of nuclear HaloTag7-SNAP-tag2 and nuclear 
            mTurquoise2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7441)
  AUTHORS   Kuhn S, Nasufovic V, Wilhelm J, Kompa J, de Lange EMF, Lin YH, 
            Egoldt C, Fischer J, Lennoi A, Tarnawski M, Reinstein J, Vlijm R, 
            Hiblot J, Johnsson K
  TITLE     SNAP-tag2 for faster and brighter protein labeling.
  JOURNAL   Nat Chem Biol. 2025 Jul 3. doi: 10.1038/s41589-025-01942-z.
  PUBMED    40610720
REFERENCE   2  (bases 1 to 7441)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem 
            Biol. 2025 Jul 3. doi: 10.1038/s41589-025-01942-z."
COMMENT     Sequence Label: 
            pcDNA5/FRT_HaloTag7-SNAP-tag2-NLS-P2A-NLS-mTurquoise2
FEATURES             Location/Qualifiers
     source          1..7441
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     37..56
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(223..1245)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     CDS             complement(223..1245)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     protein_bind    complement(1253..1300)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     1432..1451
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     polyA_signal    1584..1808
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     1797..1814
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     CDS             complement(join(1849..2562,2563..2565,2566..2568))
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Goedhart et 
                     al., 2012)"
                     /label=mTurquoise2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYFSDNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1894..1915)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     2243..2262
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2502..2523
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     CDS             complement(2569..2589)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(2602..2658)
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             complement(2701..2721)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(2725..2745)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(3397..4281)
                     /codon_start=1
                     /product="modified bacterial dehalogenase that forms 
                     covalent bonds with chloroalkane derivatives"
                     /label=HaloTag(R)
                     /translation="EIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPTSSYV
                     WRNIIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVIHD
                     WGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLIIDQ
                     NVFIEGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVALVEE
                     YMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPGLNLLQEDNPDLIG
                     SEIARWLSTLEISG"
     primer_bind     complement(4325..4344)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4326..4344)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(4389..4592)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(4418..4438)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        4593..4972
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     5144..5163
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        5238..5342
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5343..5411,5412..6203)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5561..5580)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6374..6962
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6863..6882
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7116..7133
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7250..7271
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7286..7291,7292..7309,7310..7316)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7324..7340
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7329..7351
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7348..7364
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7348..7364
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     polyA_signal    join(7413..7441,1..93)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(join(7424..7441,1..2))
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 acaactagaa tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta
       61 tttgtaacca ttataagctg caataaacaa gttggggtgg gcgaagaact ccagcatgag
      121 atccccgcgc tggaggatca tccagccggc gtcccggaaa acgattccga agcccaacct
      181 ttcatagaag gcggcggtgg aatcgaaatc tcgtagtacg tgctattcct ttgccctcgg
      241 acgagtgctg gggcgtcggt ttccactatc ggcgagtact tctacacagc catcggtcca
      301 gacggccgcg cttctgcggg cgatttgtgt acgcccgaca gtcccggctc cggatcggac
      361 gattgcgtcg catcgaccct gcgcccaagc tgcatcatcg aaattgccgt caaccaagct
      421 ctgatagagt tggtcaagac caatgcggag catatacgcc cggagccgcg gcgatcctgc
      481 aagctccgga tgcctccgct cgaagtagcg cgtctgctgc tccatacaag ccaaccacgg
      541 cctccagaag aagatgttgg cgacctcgta ttgggaatcc ccgaacatcg cctcgctcca
      601 gtcaatgacc gctgttatgc ggccattgtc cgtcaggaca ttgttggagc cgaaatccgc
      661 gtgcacgagg tgccggactt cggggcagtc ctcggcccaa agcatcagct catcgagagc
      721 ctgcgcgacg gacgcactga cggtgtcgtc catcacagtt tgccagtgat acacatgggg
      781 atcagcaatc gcgcatatga aatcacgcca tgtagtgtat tgaccgattc cttgcggtcc
      841 gaatgggccg aacccgctcg tctggctaag atcggccgca gcgatcgcat ccatggcctc
      901 cgcgaccggc tgcagaacag cgggcagttc ggtttcaggc aggtcttgca acgtgacacc
      961 ctgtgcacgg cgggagatgc aataggtcag gctctcgctg aattccccaa tgtcaagcac
     1021 ttccggaatc gggagcgcgg ccgatgcaaa gtgccgataa acataacgat ctttgtagaa
     1081 accatcggcg cagctattta cccgcaggac atatccacgc cctcctacat cgaagctgaa
     1141 agcacgagat tcttcgccct ccgagagctg catcaggtcg gagacgctgt cgaacttttc
     1201 gatcagaaac ttctcgacag acgtcgcggt gagttcaggc tttttggcca aggaagttcc
     1261 tatactttct agagaatagg aacttcggaa taggaacttc taggtacgtg aaccatcacc
     1321 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
     1381 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
     1441 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
     1501 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtgg ggataccccc tagagcccca
     1561 gctggttctt tccgcctcag aagccataga gcccaccgca tccccagcat gcctgctatt
     1621 gtcttcccaa tcctccccct tgctgtcctg ccccacccca ccccccagaa tagaatgaca
     1681 cctactcaga caatgcgatg caatttcctc attttattag gaaaggacag tgggagtggc
     1741 accttccagg gtcaaggaag gcacggggga ggggcaaaca acagatggct ggcaactaga
     1801 aggcacagtc gaggctgatc agcgggttta aacgggccct ctagatcatt acttgtacag
     1861 ctcgtccatg ccgagagtga tcccggcggc ggtcacgaac tccagcagga ccatgtgatc
     1921 gcgcttctcg ttggggtctt tgctcagctt ggactgggtg ctcaggtagt ggttgtcggg
     1981 cagcagcacg gggccgtcgc cgatgggggt gttctgctgg tagtggtcgg cgagctgcac
     2041 gccgccgtcc tcgatgttgt ggcggatctt gaagttggcc ttgatgccgt tcttctgctt
     2101 gtcggcggtg atatagacgt tgtcgctaaa gtagttgtac tccagcttgt gccccaggat
     2161 gttgccgtcc tccttgaagt cgatgccctt cagctcgatg cggttcacca gggtgtcgcc
     2221 ctcgaacttc acctcggcgc gggtcttgta gttgccgtcg tccttgaaga agatggtgcg
     2281 ctcctggacg tagccttcgg gcatggcgga cttgaagaag tcgtgctgct tcatgtggtc
     2341 ggggtagcgg gcgaagcact gcacgcccca ggacagggtg gtcacgaggg tgggccaggg
     2401 cacgggcagc ttgccggtgg tgcagatgaa cttcagggtc agcttgccgt aggtggcatc
     2461 gccctcgccc tcgccggaca cgctgaactt gtggccgttt acgtcgccgt ccagctcgac
     2521 caggatgggc accaccccgg tgaacagctc ctcgcccttg ctcaccatca ccttcctctt
     2581 cttcttgggg gccattctag atggcccggg attctcttcg acatcccctg cttgtttcaa
     2641 cagggagaag ttagtggctt gaggagaagc ccggagctcc tttctcttct tttttggatc
     2701 tacctttctc ttcttttttg gatctacctt tctcttcttt tttggatccg gagctccgcc
     2761 caggcctggt ttacccagac ggtgaccttc gtgcgccagc agccattctt tcacgtacag
     2821 agggccttcg tagccaccta cgtcgccgtc gccctgaacc acacggtggc acgggatcag
     2881 aattggcacc ggattaccac gcagagcggt tttcaccgcc gcagttgccg ctgggttgcc
     2941 agccagcgcc gccagctggg aataggagat cacctcgccg aatttcacta ctttcagcag
     3001 tttccacagt acctgacggg taaaggattc ctgctgaaat accgggtgat gcagggccgg
     3061 tacagggaac tcttcaatcg cctctggctg gtgaaaatat gcgttcagcc atgcggtagc
     3121 ctgcatcagc ggctccgggc ccggaggacc ctgctcgccc tgaccattgc ccacgaaata
     3181 gatgcggtgc aggccctgct cgcaaccgct cagcagcagt ttacccagcg ggctgtcata
     3241 ggtagtgcgt ttcatttcgc agtctttatc catctcgaga gaccgagagc ggccgccagg
     3301 tggtggaggc ggtggcggtg gtggtggagg aggcggagga ggtggtggtg gcggcggcgg
     3361 cggtggtggc ggtggcggag gaggagggcg gccgctaccg gaaatctcca gagtagacag
     3421 ccagcgcgcg atctcgctgc cgatcaggtc cgggttgtct tcttgcagca gattcagacc
     3481 cgggccgatg tccacagcct tgcagttagg caggcttttg gccaggcgag cggcttcggc
     3541 cggtgggatc agaacgcctg gggtgcccca gaacagcagc ttcgggacag gggactggtg
     3601 cagccagtcc atgtattctt cgaccagcgc gacgatgttc gctggctcac cggcgattgg
     3661 cagctcgttt gggaagcgcc acagtggctc gcggtcaaca ggattcagga acggctcgcg
     3721 gtaatggtcc atctcgactt cagtcagcgg gcggacgaca cccatcggca gcgtaccctc
     3781 gataaaaacg ttctgatcga tgatcagctt gcggccgacg tcggtggtgc ggaaggcctg
     3841 gaaggtctcg cgggcaaatt ctggccattc gtcccaggtc gggatagggc ggatgaactc
     3901 cataaatgca atacctttga cgcgctctgg attgcgcttg gcccagtgga aacccagagc
     3961 ggagccccag tcgtgaatga ccaggacgac ctcttccaga cccagggctt cgatgaaggc
     4021 atccatgaag cggacgtggt cgtcgaagaa ataacccagg tctggtttgt cggatttgcc
     4081 cataccgatc aggtctggag caatgcagcg atgggtcggt gcaacatgcg ggatgatgtt
     4141 gcgccacacg taggaggagg tcgggttacc gtgcaggaac agcacagggg tgccatcgcg
     4201 cggaccaaca tcgacgtagt gcatgcgctc gcccaggact tccacataat gggggtcgaa
     4261 tggaaagcca gtaccgattt cggatcccat ggtggtaagt ttaaacgcta gccagcttgg
     4321 gtctccctat agtgagtcgt attaatttcg ataagccagt aagcagtggg ttctctagtt
     4381 agccagagag ctctgcttat atagacctcc caccgtacac gcctaccgcc catttgcgtc
     4441 aatggggcgg agttgttacg acattttgga aagtcccgtt gattttggtg ccaaaacaaa
     4501 ctcccattga cgtcaatggg gtggagactt ggaaatcccc gtgagtcaaa ccgctatcca
     4561 cgcccattga tgtactgcca aaaccgcatc accatggtaa tagcgatgac taatacgtag
     4621 atgtactgcc aagtaggaaa gtcccataag gtcatgtact gggcataatg ccaggcgggc
     4681 catttaccgt cattgacgtc aatagggggc gtacttggca tatgatacac ttgatgtact
     4741 gccaagtggg cagtttaccg taaatactcc acccattgac gtcaatggaa agtccctatt
     4801 ggcgttacta tgggaacata cgtcattatt gacgtcaatg ggcgggggtc gttgggcggt
     4861 cagccaggcg ggccatttac cgtaagttat gtaacgcgga actccatata tgggctatga
     4921 actaatgacc ccgtaattga ttactattaa taactagtca ataatcaatg tcaacgcgta
     4981 tatctggccc gtacatcgcg aagcagcgca aaacgcctaa ccctaagcag attcttcatg
     5041 caattgtcgg tcaagccttg ccttgttgta gcttaaattt tgctcgcgca ctactcagcg
     5101 acctccaaca cacaagcagg gagcagatac tggcttaact atgcggcatc agagcagatt
     5161 gtactgagag tgcaccatag gggatcggga gatctcccga tccgtcgacg tcaggtggca
     5221 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata
     5281 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga
     5341 gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc
     5401 ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg
     5461 cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc
     5521 ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat
     5581 cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact
     5641 tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat
     5701 tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga
     5761 tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc
     5821 ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga
     5881 tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag
     5941 cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc
     6001 gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt
     6061 ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct
     6121 acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg
     6181 cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg
     6241 atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca
     6301 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
     6361 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
     6421 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
     6481 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
     6541 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
     6601 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
     6661 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
     6721 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
     6781 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
     6841 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     6901 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
     6961 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     7021 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
     7081 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     7141 aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct
     7201 ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt
     7261 agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg
     7321 gaattgtgag cggataacaa tttcacacag gaaacagcta tgaccatgat tacgccaagc
     7381 tctagctaga ggtcgacggt atacagacat gataagatac attgatgagt ttggacaaac
     7441 c
//
