LOCUS       sequence-449284-       11924 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression plasmid encoding STING-mNeonGreen under hPGK 
            promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11924)
  AUTHORS   Gentili M, Carlson RJ, Liu B, Hellier Q, Andrews J, Qin Y, Blainey 
            PC, Hacohen N
  TITLE     Classification and functional characterization of regulators of 
            intracellular STING trafficking identified by genome-wide optical 
            pooled screening.
  JOURNAL   Cell Syst. 2024 Dec 18;15(12):1264-1277.e8. doi: 
            10.1016/j.cels.2024.11.004. Epub 2024 Dec 9.
  PUBMED    39657680
REFERENCE   2  (bases 1 to 11924)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cels.2024.11.004"; journalName: "Cell Syst"; date: 
            "2024-12-18- 18"; volume: "15"; issue: "12"; pages: "1264-1277.e8"
COMMENT     Sequence Label: pTRIP-hPGK-Blast-2A-STING-mNeonGreen
FEATURES             Location/Qualifiers
     source          1..11924
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     intron          43..108
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             238..258
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    683..817
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(720..739)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     774..793
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             985..1618
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    1665..1790
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2283..2516
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2701..2745
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    3038..3155
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        3203..3713
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     3632..3650
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     regulatory      3726..3735
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3732..4127
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     CDS             4143..4199
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     LTR             6328..6508
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(8036..8055)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(8269..8288)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8388..8410
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(8448..8466)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8534..8638
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8639..8707,8708..9499)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8857..8876)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9670..10258
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10159..10178
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10412..10429
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(10499..10519)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        10504..10833
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      10684..10819
                     /label=SV40 ori
                     /note="SV40 origin of replication"
ORIGIN
        1 acataattgg acaaactacc tacagagatt taaagctcta aggtaaatat aaaattttta
       61 agtgtataat gtgttaaact actgattcta attgtttgtg tattttagat tccaacctat
      121 ggaactgatg aatgggagca gtggtggaat gcctttaatg aggaaaacct gttttgctca
      181 gaagaaatgc catctagtga tgatgaggct actgctgact ctcaacattc tactcctcca
      241 aaaaagaaga gaaaggtaga agaccccaag gactttcctt cagaattgct aagttttttg
      301 agtcatgctg tgtttagtaa tagaactctt gcttgctttg ctatttacac cacaaaggaa
      361 aaagctgcac tgctatacaa gaaaattatg gaaaaatatt ctgtaacctt tataagtagg
      421 cataacagtt ataatcataa catactgttt tttcttactc cacacaggca tagagtgtct
      481 gctattaata actatgctca aaaattgtgt acctttagct ttttaatttg taaaggggtt
      541 aataaggaat atttgatgta tagtgccttg actagagatc ataatcagcc ataccacatt
      601 tgtagaggtt ttacttgctt taaaaaacct cccacacctc cccctgaacc tgaaacataa
      661 aatgaatgca attgttgttg ttaacttgtt tattgcagct tataatggtt acaaataaag
      721 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
      781 gtccaaactc atcaatgtat cttatcatgt ctggatcaac tggataactc aagctaacca
      841 aaatcatccc aaacttccca ccccataccc tattaccact gccaattacc tgtggtttca
      901 tttactctaa acctgtgatt cctctgaatt attttcattt taaagaaatt gtatttgtta
      961 aatatgtact acaaacttag tagttggaag ggctaattca ctcccaaaga agacaagata
     1021 tccttgatct gtggatctac cacacacaag gctacttccc tgattagcag aactacacac
     1081 cagggccagg ggtcagatat ccactgacct ttggatggtg ctacaagcta gtaccagttg
     1141 agccagataa ggtagaagag gccaataaag gagagaacac cagcttgtta caccctgtga
     1201 gcctgcatgg gatggatgac ccggagagag aagtgttaga gtggaggttt gacagccgcc
     1261 tagcatttca tcacgtggcc cgagagctgc atccggagta cttcaagaac tgctgatatc
     1321 gagcttgcta caagggactt tccgctgggg actttccagg gaggcgtggc ctgggcggga
     1381 ctggggagtg gcgagccctc agatcctgca tataagcagc tgctttttgc ctgtactggg
     1441 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
     1501 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
     1561 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt
     1621 ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga
     1681 ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa
     1741 aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc
     1801 gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat
     1861 ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg
     1921 gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc
     1981 agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc
     2041 atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa
     2101 acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat
     2161 atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta
     2221 ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga
     2281 ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca
     2341 atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat
     2401 ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag
     2461 cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg
     2521 atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg
     2581 agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa
     2641 attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa
     2701 aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac
     2761 ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt
     2821 ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca
     2881 ttatcgtttc agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa
     2941 gaagaaggtg gagagagaga cagagacaga tccattcgat tagtgaacgg atctcgacgg
     3001 tatcgccgaa ttcacaaatg gcagtattca tccacaattt taaaagaaaa ggggggattg
     3061 gggggtacag tgcaggggaa agaatagtag acataatagc aacagacata caaactaaag
     3121 aattacaaaa acaaattaca aaaattcaaa attttcgggt ttattacagg gacagcagag
     3181 atccactttg gctgatacgc gtggggttgg ggttgcgcct tttccaaggc agccctgggt
     3241 ttgcgcaggg acgcggctgc tctgggcgtg gttccgggaa acgcagcggc gccgaccctg
     3301 ggtctcgcac attcttcacg tccgttcgca gcgtcacccg gatcttcgcc gctacccttg
     3361 tgggcccccc ggcgacgctt cctgctccgc ccctaagtcg ggaaggttcc ttgcggttcg
     3421 cggcgtgccg gacgtgacaa acggaagccg cacgtctcac tagtaccctc gcagacggac
     3481 agcgccaggg agcaatggca gcgcgccgac cgcgatgggc tgtggccaat agcggctgct
     3541 cagcagggcg cgccgagagc agcggccggg aaggggcggt gcgggaggcg gggtgtgggg
     3601 cggtagtgtg ggccctgttc ctgcccgcgc ggtgttccgc attctgcaag cctccggagc
     3661 gcacgtcggc agtcggctcc ctcgttgacc gaatcaccga cctctctccc cagccgcggg
     3721 ctagcgccac catggccaag cctttgtctc aagaagaatc caccctcatt gaaagagcaa
     3781 cggctacaat caacagcatc cccatctctg aagactacag cgtcgccagc gcagctctct
     3841 ctagcgacgg ccgcatcttc actggtgtca atgtatatca ttttactggg ggaccttgtg
     3901 cagaactcgt ggtgctgggc actgctgctg ctgcggcagc tggcaacctg acttgtatcg
     3961 tcgcgatcgg aaatgagaac aggggcatct tgagcccctg cggacggtgc cgacaggtgc
     4021 ttctcgatct gcatcctggg atcaaagcca tagtgaagga cagtgatgga cagccgacgg
     4081 cagttgggat tcgtgaattg ctgccctctg gttatgtgtg ggagggccgt acgggaagcg
     4141 gagctactaa cttcagcctg ctgaagcagg ctggagacgt ggaggagaac cctggacctg
     4201 gatccatgcc ccactccagc ctgcatccat ccatcccgtg tcccaggggt cacggggccc
     4261 agaaggcagc cttggttctg ctgagtgcct gcctggtgac cctttggggg ctaggagagc
     4321 caccagagca cactctccgg tacctggtgc tccacctagc ctccctgcag ctgggactgc
     4381 tgttaaacgg ggtctgcagc ctggctgagg agctgcgcca catccactcc aggtaccggg
     4441 gcagctactg gaggactgtg cgggcctgcc tgggctgccc cctccgccgt ggggccctgt
     4501 tgctgctgtc catctatttc tactactccc tcccaaatgc ggtcggcccg cccttcactt
     4561 ggatgcttgc cctcctgggc ctctcgcagg cactgaacat cctcctgggc ctcaagggcc
     4621 tggccccagc tgagatctct gcagtgtgtg aaaaagggaa tttcaacgtg gcccatgggc
     4681 tggcatggtc atattacatc ggatatctgc ggctgatcct gccagagctc caggcccgga
     4741 ttcgaactta caatcagcat tacaacaacc tgctacgggg tgcagtgagc cagcggctgt
     4801 atattctcct cccattggac tgtggggtgc ctgataacct gagtatggct gaccccaaca
     4861 ttcgcttcct ggataaactg ccccagcaga ccggtgaccg tgctggcatc aaggatcggg
     4921 tttacagcaa cagcatctat gagcttctgg agaacgggca gcgggcgggc acctgtgtcc
     4981 tggagtacgc cacccccttg cagactttgt ttgccatgtc acaatacagt caagctggct
     5041 ttagccggga ggataggctt gagcaggcca aactcttctg ccggacactt gaggacatcc
     5101 tggcagatgc ccctgagtct cagaacaact gccgcctcat tgcctaccag gaacctgcag
     5161 atgacagcag cttctcgctg tcccaggagg ttctccggca cctgcggcag gaggaaaagg
     5221 aagaggttac tgtgggcagc ttgaagacct cagcggtgcc cagtacctcc acgatgtccc
     5281 aagagcctga gctcctcatc agtggaatgg aaaagcccct ccctctccgc acggatttct
     5341 cttctagaat ggtcagcaaa ggcgaagaag acaacatggc atcactgcca gcgacacacg
     5401 aacttcatat tttcggctcc atcaatggtg tggacttcga tatggttgga cagggtactg
     5461 ggaaccctaa cgatggatat gaggagttga acttgaagag taccaagggc gatcttcagt
     5521 tttcaccttg gattctcgta cctcatatag gttatggctt tcatcaatat ctgccctatc
     5581 ctgacggtat gtcacctttc caagcagcta tggttgacgg gagcggttat caggttcacc
     5641 ggacaatgca gtttgaagat ggagcctctt tgaccgtaaa ttatcggtat acttatgagg
     5701 gcagccatat taaaggcgag gctcaagtta aggggactgg gttccccgcg gatggtcccg
     5761 taatgacaaa cagtctgacg gctgcagact ggtgccgctc taagaagaca tatccaaatg
     5821 acaagacgat tattagcact ttcaagtggt catacactac aggaaatggt aaacgctata
     5881 ggagcactgc ccggaccacg tatactttcg cgaagccgat ggcggcaaat tatttgaaaa
     5941 accaaccgat gtatgtgttc agaaagacag aactcaagca ttctaaaact gaactgaact
     6001 tcaaagagtg gcagaaagcc tttactgatg taatgggcat ggacgaactc tataagtaac
     6061 tcgagaccta gaaaaacatg gagcaatcac aagtagcaat acagcagcta ccaatgctga
     6121 ttgtgcctgg ctagaagcac aagaggagga ggaggtgggt tttccagtca cacctcaggt
     6181 acctttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa
     6241 ggggggactg gaagggctaa ttcactccca acgaagacaa aatcgtcgag agatgctgca
     6301 tataagcagc tgctttttgc ttgtactggg tctctctggt tagaccagat ctgagcctgg
     6361 gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg
     6421 cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc
     6481 ttttagtcag tgtggaaaat ctctagcagt agtagttcat gtcatcttat tattcagtat
     6541 ttataacttg caaagaaatg aatatcagag agtgagaggc cttgacatta taatagattt
     6601 agcaggaatt gaactaggag tggagcacac aggcaaagct gcagaagtac ttggaagaag
     6661 ccaccagaga tactcacgat tctgcacata cctggctaat cccagatcct aaggattaca
     6721 ttaagtttac taacatttat ataatgattt atagtttaaa gtataaactt atctaattta
     6781 ctattctgac agatattaat taatcctcaa atatcataag agatgattac tattatcccc
     6841 atttaacaca agaggaaact gagagggaaa gatgttgaag taattttccc acaattacag
     6901 catccgttag ttacgactct atgatcttct gacacaaatt ccatttactc ctcaccctat
     6961 gactcagtcg aatatatcaa agttatggac attatgctaa gtaacaaatt acccttttat
     7021 atagtaaata ctgagtagat tgagagaaga aattgtttgc aaacctgaat agcttcaaga
     7081 agaagagaag tgaggataag aataacagtt gtcatttaac aagttttaac aagtaacttg
     7141 gttagaaagg gattcaaatg cataaagcaa gggataaatt tttctggcaa caagactata
     7201 caatataacc ttaaatatga cttcaaataa ttgttggaac ttgataaaac taattaaata
     7261 ttattgaaga ttatcaatat tataaatgta atttactttt aaaaagggaa catagaaatg
     7321 tgtatcatta gagtagaaaa caatccttat tatcacaatt tgtcaaaaca agtttgttat
     7381 taacacaagt agaatactgc attcaattaa gttgactgca gattttgtgt tttgttaaaa
     7441 ttagaaagag ataacaacaa tttgaattat tgaaagtaac atgtaaatag ttctacatac
     7501 gttcttttga catcttgttc aatcattgat cgaagttctt tatcttggaa gaatttgttc
     7561 caaagactct gaaataagga aaacaatcta ttatatagtc tcacaccttt gttttacttt
     7621 tagtgatttc aatttaataa tgtaaatggt taaaatttat tcttctctga gatcatttca
     7681 cattgcagat agaaaacctg agactggggt aatttttatt aaaatctaat ttaatctcag
     7741 aaacacatct ttattctaac atcaattttt ccagtttgat attatcatat aaagtcagcc
     7801 ttcctcatct gcaggttcca caacaaaaat ccaaccaact gtggatcaaa aatattggga
     7861 aaaaattaaa aatagcaata caacaataaa aaaatacaaa tcagaaaaac agcacagtat
     7921 aacaacttta tttagcattt acaatctatt aggtattata agtaatctag ccagatcctc
     7981 tacgccggac gcatcgtggc cggcatcacc ggcgccacag gtgcggttgc tggcgcctat
     8041 atcgccgaca tcaccgatgg ggaagatcgg gctcgccact tcgggctcat gagcgcttgt
     8101 ttcggcgtgg gtatggtggc aggccccgtg gccgggggac tgttgggcgc catctccttg
     8161 catgcaccat tccttgcggc ggcggtgctc aacggcctca acctactact gggctgcttc
     8221 ctaatgcagg agtcgcataa gggagagcgt cgaatggtgc actctcagta caatctgctc
     8281 tgatgccgca tagttaagcc agccccgaca cccgccaaca cccgctgacg cgccctgacg
     8341 ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat
     8401 gtgtcagagg ttttcaccgt catcaccgaa acgcgcgaga cgaaagggcc tcgtgatacg
     8461 cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt
     8521 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
     8581 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat
     8641 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt
     8701 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg
     8761 agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga
     8821 agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg
     8881 tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt
     8941 tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg
     9001 cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg
     9061 aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga
     9121 tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc
     9181 tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc
     9241 ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc
     9301 ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg
     9361 cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac
     9421 gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc
     9481 actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt
     9541 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
     9601 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
     9661 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     9721 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     9781 aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg
     9841 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     9901 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     9961 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
    10021 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
    10081 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
    10141 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
    10201 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
    10261 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
    10321 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
    10381 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
    10441 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctgtgg
    10501 aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa
    10561 agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc
    10621 agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg
    10681 cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt
    10741 ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga
    10801 ggaggctttt ttggaggcct aggcttttgc aaaaagcttg gacacaagac aggcttgcga
    10861 gatatgtttg agaataccac tttatcccgc gtcagggaga ggcagtgcgt aaaaagacgc
    10921 ggactcatgt gaaatactgg tttttagtgc gccagatctc tataatctcg cgcaacctat
    10981 tttcccctcg aacacttttt aagccgtaga taaacaggct gggacacttc acatgagcga
    11041 aaaatacatc gtcacctggg acatgttgca gatccatgca cgtaaactcg caagccgact
    11101 gatgccttct gaacaatgga aaggcattat tgccgtaagc cgtggcggtc tgtaccgggt
    11161 gcgttactgg cgcgtgaact gggtattcgt catgtcgata ccgtttgtat ttccagctac
    11221 gatcacgaca accagcgcga gcttaaagtg ctgaaacgcg cagaaggcga tggcgaaggc
    11281 ttcatcgtta ttgatgacct ggtggatacc ggtggtactg cggttgcgat tcgtgaaatg
    11341 tatccaaaag cgcactttgt caccatcttc gcaaaaccgg ctggtcgtcc gctggttgat
    11401 gactatgttg ttgatatccc gcaagatacc tggattgaac agccgtggga tatgggcgtc
    11461 gtattcgtcc cgccaatctc cggtcgctaa tcttttcaac gcctggcact gccgggcgtt
    11521 gttcttttta acttcaggcg ggttacaata gtttccagta agtattctgg aggctgcatc
    11581 catgacacag gcaaacctga gcgaaaccct gttcaaaccc cgctttaaac atcctgaaac
    11641 ctcgacgcta gtccgccgct ttaatcacgg cgcacaaccg cctgtgcagt cggcccttga
    11701 tggtaaaacc atccctcact ggtatcgcat gattaaccgt ctgatgtgga tctggcgcgg
    11761 cattgaccca cgcgaaatcc tcgacgtcca ggcacgtatt gtgatgagcg atgccgaacg
    11821 taccgacgat gatttatacg atacggtgat tggctaccgt ggcggcaact ggatttatga
    11881 gtgggccccg gatctttgtg aaggaacctt acttctgtgg tgtg
//
