LOCUS       sequence-44929-e         845 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 845)
  AUTHORS   Wadia JS, Stan RV, Dowdy SF
  TITLE     Transducible TAT-HA fusogenic peptide enhances escape of TAT-fusion 
            proteins after lipid raft macropinocytosis.
  JOURNAL   Nat Med. 2004 Mar;10(3):310-5. Epub 2004 Feb 8.
  PUBMED    14770178
REFERENCE   2  (bases 1 to 845)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Med."; 
            date: "2004-03"; volume: "10(3)"; pages: "310-5. Epub 2004 Feb 8"
COMMENT     Sequence Label: pTAT2.2
FEATURES             Location/Qualifiers
     source          1..845
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             72..104
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             153..170
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(223..241)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      237..284
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      321..776
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(408..427)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     618..639
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 atattttgtt tactttaaga aggagatata ccatgggcag gaagaagcgg agacagcgac
       61 gaagaggcca tatggctagc atgactggtg gacagcaaat gggtcgggat ccgaattcga
      121 gctccgtcga caagcttgcg gccgcactcg agcaccacca ccaccaccac tgagatccgg
      181 ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag
      241 cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta
      301 tatccggatt ggcgaatggg acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt
      361 ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt
      421 cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct
      481 ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg
      541 tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga
      601 gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc
      661 ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga
      721 gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgctta caatttaggt
      781 ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca
      841 aatat
//
