LOCUS       sequence-449293-         753 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression of the Coxsackievirus A16 (G-10) 2A protease 
            this construct can be used for biochemical assay and to obtain 
            crystals in the space group C1 2 1(PDB 8POA).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 753)
  TITLE     ASAP Discovery Plasmids 2024
REFERENCE   2  (bases 1 to 753)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: Human Coxsackievirus A16 strain G10 2A protease
FEATURES             Location/Qualifiers
     source          1..753
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4..21
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 atgcaccacc atcatcacca tggctcagga gatcaggaag cgaaaccttc gaccgaggac
       61 ctgggcgaca agaaagaagg ggagtatatt aagttaaagg tgatcggcca ggactcaagc
      121 gagatccact tcaaagtcaa aatgaccacg cacctcaaga agttgaagga gagttactgt
      181 caacggcaag gggttcctat gaactcgctt cgcttcctgt tcgagggcca gcgaattgcg
      241 gacaaccaca cacctaagga acttggtatg gaagaggaag acgtcattga ggtgtaccag
      301 gagcagacag gcggctcggg tgccatatat gtgggtaact atcgtgtggt taatcgccac
      361 ctcgctactc acaatgattg ggccaactta gtgtgggagg acagctctcg cgatctcctt
      421 gtatcatcga ccactgcgca gggttgcgac accattgcgc gctgtgattg ccagacaggc
      481 gtgtattatt gctcgtctcg tcgtaaacat tatccggttt ctttttctaa gccgtcactg
      541 atctttgttg aagcgtcaga gtactatcca gcccgttatc agtcccatct gatgctggct
      601 gtgggtcaca gcgaacccgg ggattgtggc ggtatactgc gctgtcagca cggtgtagtt
      661 ggcattgtca gtacaggagg taatgggtta gtcggatttg ccgatgtgcg ggatttactg
      721 tggttggacg aggaggctat ggaacagtaa tga
//
