LOCUS       sequence-449295-         498 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression of the A71 2A protease for assays that require 
            immobilization using biotin-streptavidin interaction.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 498)
  TITLE     ASAP Discovery Plasmids 2024
REFERENCE   2  (bases 1 to 498)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: Enterovirus A71 (EV-A71) isolate 4643-TW98 2A
            ...
FEATURES             Location/Qualifiers
     source          1..498
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             436..453
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             454..498
                     /codon_start=1
                     /product="peptide tag that allows for enzymatic 
                     biotinylation"
                     /label=AviTag(TM)
                     /translation="GLNDIFEAQKIEWHE"
ORIGIN
        1 atgtcgggtg ccatatatgt gggtaactat cgtgtggtta atcgccacct cgctactcac
       61 aatgattggg ccaacttagt gtgggaggac agctctcgcg atctccttgt atcatcgacc
      121 actgcgcagg gttgcgacac cattgcgcgc tgtgattgcc agacaggcgt gtattattgc
      181 tcgtctcgtc gtaaacatta tccggtttct ttttctaagc cgtcactgat ctttgttgaa
      241 gcgtcagagt actatccagc ccgttatcag tcccatctga tgctggctgt gggtcacagc
      301 gaacccgggg attgtggcgg tatactgcgc tgtcagcacg gtgtagttgg cattgtcagt
      361 acaggaggta atgggttagt cggatttgcc gatgtgcggg atttactgtg gttggacgag
      421 gaggctatgg aacagcatca tcaccatcac cacggcctga acgatatttt tgaagcgcag
      481 aaaattgaat ggcatgaa
//
