LOCUS       sequence-449296-         621 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression of the A71 3C protease for assays that require 
            immobilization using biotin-streptavidin interaction.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 621)
  TITLE     ASAP Discovery Plasmids 2024
REFERENCE   2  (bases 1 to 621)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: Enterovirus A71 (EV-A71) isolate 4643-TW98 3C
            ...
FEATURES             Location/Qualifiers
     source          1..621
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             559..576
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             577..621
                     /codon_start=1
                     /product="peptide tag that allows for enzymatic 
                     biotinylation"
                     /label=AviTag(TM)
                     /translation="GLNDIFEAQKIEWHE"
ORIGIN
        1 atgggtccta gcctggattt tgcgttgtca ttgctgcgcc gtaatatcag acaggttcag
       61 accgatcagg gccatttcac catgctcgga gttcgcgatc gtttggcggt gctcccgcgt
      121 cactctcagc caggtaaaac gatttgggtt gaacataaat taataaacat attggacgcc
      181 gttgagctgg ttgatgaaca gggtgtaaac ttagaactga ctttagtgac cctggatacc
      241 aatgaaaagt ttcgcgacat taccaaattt atccccgaaa atatctcagc cgcctcagat
      301 gccacactgg ttatcaacac tgagcacatg cccagcatgt ttgtcccggt tggagatgta
      361 gttcagtacg gttttcttaa cctgagcggt aagcctacac atcgtaccat gatgtataac
      421 tttccgacga aggctggtca gtgtgggggt gtggttacga gtgttggtaa agtgatcggg
      481 atacatatag gagggaatgg tcgtcagggc ttttgcgctg gtctgaaacg ttcgtatttt
      541 gcatcagaac agcttgagca ccatcatcac caccatggcc tgaacgatat ttttgaagcg
      601 cagaaaattg aatggcatga a
//
