LOCUS       sequence-449302-         417 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression of the SARS-CoV-2 N protein C terminal 
            oligomerization domain used for crystallization assays. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 417)
  TITLE     ASAP Discovery Plasmids 2024
REFERENCE   2  (bases 1 to 417)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: SARS-CoV-2 isolate:Wuhan-Hu-1 Nucleocapsid Pro...
FEATURES             Location/Qualifiers
     source          1..417
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4..21
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             46..66
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 atgcaccatc atcatcatca ttcttctggt gtagatctgg gtaccgagaa cctgtacttc
       61 caatccatga gcgcagcaga agcaagcaaa aaaccgcgtc agaaacgtac cgcaaccaaa
      121 gcatataatg ttacccaggc atttggtcgt cgtggtccgg aacagaccca gggtaatttt
      181 ggtgatcaag aactgattcg tcagggcacc gattataaac attggcctca gattgcacag
      241 tttgcaccga gcgcaagtgc attttttggc atgagccgta ttggtatgga agttaccccg
      301 agcggcacct ggctgaccta tacaggtgca attaaactgg atgataaaga tccgaacttc
      361 aaggatcagg tgattctgct gaacaaacat atcgatgcct ataaaacatt tccgtaa
//
