LOCUS       sequence-449304-         615 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression of the SARS-CoV-2 nsp3 macrodomain for 
            crystallization of SARS-CoV-2 nsp3 macrodomain in P 4 3.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 615)
  TITLE     ASAP Discovery Plasmids 2024
REFERENCE   2  (bases 1 to 615)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: SARS-CoV-2 isolate:Wuhan-Hu-1 nsp3 macrodomain
FEATURES             Location/Qualifiers
     source          1..615
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             13..30
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     protein_bind    40..64
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             67..90
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
ORIGIN
        1 atgtcgtact accatcacca tcaccatcac ctcgaatcaa caagtttgta caaaaaagca
       61 ggcttcctgg aagttctgtt tcagggtcct gaagttaata gctttagcgg ttatctgaaa
      121 ctgaccgata acgtgtatat caaaaacgcc gatattgtgg aagaggccaa aaaagttaaa
      181 ccgaccgttg ttgttaatgc agccaatgtt tacctgaaac atggtggtgg tgttgccggt
      241 gcactgaata aagcaaccaa taatgcaatg caggttgaga gcgacgatta tattgcaacc
      301 aatggtccgc tgaaagttgg tggtagctgt gttctgagcg gtcataatct ggcaaaacac
      361 tgtctgcatg ttgttggtcc gaatgtgaat aaaggtgaag atattcagct gctgaaaagc
      421 gcctatgaaa actttaatca gcatgaagtt ctgctggcac cgctgctgag cgcaggtatt
      481 tttggtgcag atccgattca tagcctgcgt gtttgtgttg ataccgttcg taccaatgtg
      541 tatctggcag tgtttgataa aaacctgtat gacaaactgg tgagcagctt cttagaaatg
      601 aaaagcgaga aataa
//
