LOCUS       sequence-449305-         888 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression of the D68 3C protease.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 888)
  TITLE     ASAP Discovery Plasmids 2024
REFERENCE   2  (bases 1 to 888)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: Enterovirus D68 (EV-D68) strain EV-D68 STL 201...
FEATURES             Location/Qualifiers
     source          1..888
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             13..30
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 atgggctctt cgcatcatca tcaccatcac atggcaagca tgtcagacag cgaggttaat
       61 caagaggcta agccagaggt aaagcctgag gtgaaacctg agactcacat caatttaaag
      121 gtgtcagatg gctcgtctga gatattcttc aagattaaga agacaacccc actcagacgg
      181 ttaatggaag catttgcaaa gcgccagggc aaggagatgg actcattgcg cttcctgtac
      241 gatggcattc gcattcaagc ggaccagaca ccagaagatc ttgatatgga agataacgac
      301 attatagagg ctcaccgtga gcaaataggt ggtgggcctg gtttcgactt cgcgcaagcc
      361 atcatgaaga agaacacagt gatagcgcga acggagaaag gggaattcac catgcttggg
      421 gtgtacgatc gcgttgccgt gatccctacc catgcctcgg tcggagagac gatctatata
      481 aatgacgtcg agacgaaggt gttagacgct tgtgcactgc gtgacctgac agatacaaac
      541 ctggagatta ccatagtgaa gcttgaccgg aaccaaaagt tccgtgacat cagacatttt
      601 cttcctcgat acgaagacga ctacaacgat gcggtgctct cagttcacac ctcgaaattc
      661 cctaacatgt acataccggt gggtcaagtg accaactatg ggttccttaa ccttggtggt
      721 acgccgaccc accgaatact gatgtacaac tttcccactc gggccggcca atgcggtgga
      781 gtagtgacta caacgggtaa ggtcattggc atacacgtag gtggtaatgg ggctcagggt
      841 ttcgcagcga tgttacttca ttcatacttt tcggacacgc aataatga
//
