LOCUS       sequence-449307-        1239 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression of the MERS-CoV Mpro domain for biochemical 
            assay..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1239)
  TITLE     ASAP Discovery Plasmids 2024
REFERENCE   2  (bases 1 to 1239)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: MERS-CoV Mpro strain HCoV-EMC 3CL protease domain
FEATURES             Location/Qualifiers
     source          1..1239
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4..21
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 atgcaccacc atcatcacca tggctcagga gatcaggaag cgaaaccttc gaccgaggac
       61 ctgggcgaca agaaagaagg ggagtatatt aagttaaagg tgatcggcca ggactcaagc
      121 gagatccact tcaaagtcaa aatgaccacg cacctcaaga agttgaagga gagttactgt
      181 caacggcaag gggttcctat gaactcgctt cgcttcctgt tcgagggcca gcgaattgcg
      241 gacaaccaca cacctaagga acttggtatg gaagaggaag acgtcattga ggtgtaccag
      301 gagcagacag gcggctctgg gctcgttaag atgtcccatc ctagcggaga cgtcgaggcg
      361 tgtatggtac aagttacgtg cgggagcatg acgttaaacg gattgtggct cgacaataca
      421 gtttggtgcc cacgacatgt catgtgtccg gctgatcaat taagtgaccc aaattacgac
      481 gcgttgctca tatccatgac gaaccacagc tttagcgtcc aaaagcacat tggtgcccct
      541 gctaaccttc gggtcgttgg ccatgctatg cagggcactc ttttaaagct gacagttgac
      601 gttgctaacc ctagtacgcc tgcctataca ttcactacgg ttaaacccgg cgccgcgttc
      661 tccgttttgg catgttacaa cggtcgtccg acaggtacgt tcacagtagt tatgcggcca
      721 aattatacca tcaaaggtag cttcctttgc ggttcatgtg gtagtgtggg ctacaccaaa
      781 gagggctcgg tgataaattt ctgttacatg caccaaatgg aacttgcaaa cgggacccac
      841 accggtagtg cgtttgatgg taccatgtat ggagctttca tggacaagca agttcaccaa
      901 gtgcaactga ccgataagta ctgctctgtt aatgtcgtgg cttggctgta tgccgccatc
      961 ttgaacggct gcgcctggtt tgtcaagcca aatcgcacgt ccgttgtaag cttcaacgag
     1021 tgggccctgg caaaccaatt cactgagttc gttgggaccc agtctgtcga catgttggcc
     1081 gttaagacgg gtgtagcgat agagcagctg ctttacgcaa tccaacaatt atacacaggt
     1141 ttccagggaa agcagattct cggttctaca atgttagagg acgaattcac gcccgaggac
     1201 gtgaacatgc agatcatggg cgttgtcatg caataatga
//
