LOCUS       sequence-449308-         888 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression of the Polio 3C protease.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 888)
  TITLE     ASAP Discovery Plasmids 2024
REFERENCE   2  (bases 1 to 888)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: Poliovirus 1 PV1 isolate 09_05_19 3C protease
FEATURES             Location/Qualifiers
     source          1..888
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             13..30
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 atggggtcct ctcatcatca tcaccaccat atggcttcaa tgtctgattc agaagtgaac
       61 caagaagcta agccagaagt taagccagag gttaaaccag aaactcacat aaacttgaaa
      121 gttagcgacg gatccagcga gatattcttc aagattaaga agaccacacc gctcagacgg
      181 ctcatggaag cgttcgcaaa gcgccaaggg aaggagatgg atagtttgag attcctgtac
      241 gacggtattc ggatccaagc agatcaaacc ccggaagacc tggatatgga agataacgac
      301 attattgaag cacatcgcga gcaaattggt ggtggcccgg gatttgatta cgcggttgcc
      361 atggcaaagc ggaatatagt aactgcgaca acctctaagg gcgagtttac tatgttgggt
      421 gtgcatgaca atgtggcgat cctgcctacc cacgcatcac cgggtgagtc gattgtgata
      481 gacggcaagg aagtggagat tctcgacgcc aaggccttgg aagaccaggc aggtacaaac
      541 ttagaaatta caataataac tttgaagcgc aacgagaagt ttcgcgacat ccggcctcac
      601 atacccactc aaattacaga aacgaatgac ggagtcctga tagtgaacac cagcaaatat
      661 ccgaatatgt acgtcccggt cggtgccgtt actgagcaag ggtacttaaa cctgggtgga
      721 agacaaactg ctcgcactct gatgtataac ttccctacac gcgccggtca atgtggcggt
      781 gtcatcactt gcacagggaa agtcataggc atgcatgtgg gtggaaacgg gtcccacggg
      841 tttgcggcgg cacttaagcg atcatacttc acgcaatccc aataatga
//
