LOCUS       sequence-449309-         897 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression of the fusion of the NS2B-NS3 protease from 
            Dengue virus serotype 1 for biochemical assay.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 897)
  TITLE     ASAP Discovery Plasmids 2024
REFERENCE   2  (bases 1 to 897)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: DENV-1 strain US/Hawaii/1944 NS2B-NS3 protease...
FEATURES             Location/Qualifiers
     source          1..897
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4..21
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             join(37..60,61..96,97..120)
                     /codon_start=1
                     /product="two Strep-Tag(R)II moieties connected by a linker
                     for enhanced binding to Strep-Tactin(R), an engineered form
                     of streptavidin (Schmidt et al., 2013)"
                     /label=Twin-Strep-tag
                     /translation="WSHPQFEKGGGSGGGSGGSAWSHPQFEK"
     CDS             145..165
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 atgcaccatc atcatcatca ttcttctggt gcgagctgga gtcacccaca attcgagaaa
       61 ggtggtggct ctggtggcgg ttcaggcggc tccgcctggt cccatccaca gtttgaaaag
      121 ggttctggtg tagatctggg taccgagaac ctgtacttcc aatccatggc agacttaagt
      181 cttgagaaag ccgcagaaat ctcgtgggaa gaagaggcag aacattccgg agcttcccac
      241 aatatcctgg tagaagtgca ggatgacggt acaatgaaaa tcaaagacga agagcgtgac
      301 gacacgggcg gtggcggttc tggcgggggg ggttctggtg ttttgtggga tactccttcg
      361 ccgcctgagg ttgaacgtgc tgtgctggac gacggtattt atagaatatt gcaacgaggt
      421 ctgctcggtc gctctcaagt cggagtcggt gttttccaag agggtgtgtt ccacacaatg
      481 tggcacgtga cacgtggcgc cgttttaatg taccaaggga agcgcctgga accgagctgg
      541 gccagcgtaa agaaggattt gatctcttat ggcggcggtt ggcgttttca aggaagttgg
      601 aacactgggg aagaagttca ggtcattgcc gttgagccag gcaagaatcc gaagaatgtt
      661 caaacagccc caggaacctt taaaacaccg gaaggtgaag tgggagctat agccctggac
      721 tttaagcctg gcacatctgg gtcccccata gtcaaccggg aaggcaagat tgtcggtctt
      781 tatgggaacg gggtggtcac taccagcgga acctatgtct cagcaatcgc acaagccaag
      841 gccagccaag aggggccttt accggagatc gaagatgagg tattccgtaa ataatga
//
