LOCUS       sequence-44931-e         938 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 938)
  AUTHORS   Zhang F, Cong L, Lodato S, Kosuri S, Church GM, Arlotta P
  TITLE     Efficient construction of sequence-specific TAL effectors for 
            modulating mammalian transcription.
  JOURNAL   Nat Biotechnol. 2011 Feb;29(2):149-53. doi: 10.1038/nbt.1775
  PUBMED    21248753
REFERENCE   2  (bases 1 to 938)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nbt"; 
            journalName: "Nat Biotechnol"; date: "2011-02"; volume: "29"; issue:
            "2"; pages: "149-53"
COMMENT     Sequence Label: pLenti-EF1a-OCT4
FEATURES             Location/Qualifiers
     source          1..938
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(74..223)
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     CDS             complement(248..268)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 ggccaggatt ctcctcgacg tcaccgcatg ttagcagact tcctctgccc tctccactgc
       61 ctctagagtt aatcagcatg tccaggtcga aatcatcaag ggcgtcactg ccgagcatgt
      121 cgaggtcaaa gtcatcaagg gcatccgaac caagcatgtc aaggtcaaaa tcatcgaggg
      181 cgtcacttcc cagcatatcc agatcaaaat cgtccaatgc gtcagcccgt ccggaaccgc
      241 tggcctccac ctttctcttc ttcttggggc tagctgacgc gcgagtttgg tccccttcat
      301 gcatgggcga gggcgcgtcc aaatccctct ccagcgagtc tgcgaacgca tgaaggctcg
      361 cctggtcagg tgtctgagtt gacgtaggtg aaggcttcgc gcgtttcata ccgctcgctt
      421 ggagaatcct gtcccaccgc tgggaggcgg gaggcagtgt gcccgagcgg gcctcgagtt
      481 ctgtgacacc gactctacga aagagctgca gcagtccgtg tctcgacata ccaaattgag
      541 tcatggcgtc atcgaacgct tgtgcggggt gggagtgaca ctggaagaat ccgagcacgc
      601 ggaccacttg cgcgtgatct gccactcgat gtgatgtcct ctcgggaatc cttctgttgg
      661 tccgcttaat caatgcaggc gcgtgcggga gccccttctt caccgcatcc aaggcgggtc
      721 gtccgccgag gcaggccagc gctacaagat gatcattagt gagtgcggcc agcgcggggt
      781 ccggcctcga aagctgggcc acgattgact ccagtgcggg tctgcccccg atattggatg
      841 caatagccac tacctgctca ggcgtgagtc cgtgcgcttg gcacagcaca ggcaacagcc
      901 tctgtactgt ttccaaggct tgctttcctc cgtcgtgg
//
