LOCUS       sequence-449311-         897 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression of the fusion of the NS2B-NS3 protease from 
            Dengue virus serotype 2 for biochemical assays.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 897)
  TITLE     ASAP Discovery Plasmids 2024
REFERENCE   2  (bases 1 to 897)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: DENV-2 strain Thailand/16681/84 NS2B-NS3 prote...
FEATURES             Location/Qualifiers
     source          1..897
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4..21
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             join(37..60,61..96,97..120)
                     /codon_start=1
                     /product="two Strep-Tag(R)II moieties connected by a linker
                     for enhanced binding to Strep-Tactin(R), an engineered form
                     of streptavidin (Schmidt et al., 2013)"
                     /label=Twin-Strep-tag
                     /translation="WSHPQFEKGGGSGGGSGGSAWSHPQFEK"
     CDS             145..165
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 atgcaccatc atcatcatca ttcttctggt gcgagctgga gtcacccaca attcgagaaa
       61 ggtggtggct ctggtggcgg ttcaggcggc tccgcctggt cccatccaca gtttgaaaag
      121 ggttctggtg tagatctggg taccgagaac ctgtacttcc aatccatggc agatttggag
      181 ctggagcgcg ccgcggacgt caaatgggaa gatcaagctg agatttcagg tagctcgcca
      241 atactttcta ttactatctc tgaggacggt tctatgtcca ttaagaacga ggaagaggaa
      301 caaacgctcg gcggtggtgg tagtggcggt ggtggtgcgg gtgttttgtg ggatgtaccg
      361 tctccaccac ccatggggaa agctgagtta gaggatggag cctatcgcat caagcagaag
      421 ggaatactgg gctactcaca aatcggggcc ggggtatata aagagggcac tttccacaca
      481 atgtggcacg tgacacgcgg tgccgtcctt atgcacaaag ggaagcgcat cgaaccttct
      541 tgggcggatg tgaagaagga cctcatctct tacggtggcg gttggaagtt ggaaggtgaa
      601 tggaaagagg gcgaggaagt gcaggtcctt gccctggagc ccggtaagaa tccgcgcgca
      661 gtacagacga aacccggcct gttcaagact aacgccggca ctatcggcgc cgtgtcatta
      721 gacttctcgc ccggtacttc gggatctccg atcatagata agaagggtaa ggttgtcggg
      781 ttgtacggta acggtgtcgt aacacgcagc ggggcgtacg tgagtgcaat agcccagacg
      841 gagaagagca tcgaagacaa cccagagatc gaagatgaca tattccgtaa gtaatga
//
