LOCUS       sequence-449312-         888 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression of the fusion of the NS2B-NS3 protease from 
            Dengue virus serotype 3 for biochemical assays.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 888)
  TITLE     ASAP Discovery Plasmids 2024
REFERENCE   2  (bases 1 to 888)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: DENV-3 strain Phillippines/H87/1956 NS2B-NS3
            p...
FEATURES             Location/Qualifiers
     source          1..888
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4..21
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             join(37..60,61..96,97..120)
                     /codon_start=1
                     /product="two Strep-Tag(R)II moieties connected by a linker
                     for enhanced binding to Strep-Tactin(R), an engineered form
                     of streptavidin (Schmidt et al., 2013)"
                     /label=Twin-Strep-tag
                     /translation="WSHPQFEKGGGSGGGSGGSAWSHPQFEK"
     CDS             145..165
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 atgcaccatc atcatcatca ttcttctggt gcgagctgga gtcacccaca attcgagaaa
       61 ggtggtggct ctggtggcgg ttcaggcggc tccgcctggt cccatccaca gtttgaaaag
      121 ggttctggtg tagatctggg taccgagaac ctgtacttcc aatccatggc tgatttgaca
      181 gtggaaaagg cggctgacgt aacgtgggaa gaggaagccg agcaaaccgg agttagccat
      241 aatttgatga ttacggtaga tgacgatgga acgatgagaa ttaaagatga cgagactgag
      301 aacatattag gcggcggcgg tagcgggggg ggtggatcag gagtgctctg ggatgtcccc
      361 agcccgccgg aaacacaaaa ggctgaactg gaagagggag tctatcgcat caaacagcaa
      421 ggaatatttg gaaagacaca ggttggcgtc ggtgtgcaaa aggaaggcgt tttccataca
      481 atgtggcacg taacgcgggg cgcagtactt acccacaatg gcaagcggtt agagcccaat
      541 tgggcgtctg tcaagaagga cttgatttcc tacggtggtg gctggcgctt gagcgcgcaa
      601 tggcaaaagg gtgaggaagt ccaggtgatt gcggttgagc ctggcaagaa tccaaagaac
      661 tttcaaacta tgcctggaat cttccaaacc accactggtg aaatcggcgc tatcgcactg
      721 gactttaagc ctgggacttc aggttccccc ataatcaacc gggaaggaaa ggttgttggg
      781 ctgtatggca acggcgtagt gaccaagaac ggtggatacg ttagcggcat tgctcaaaca
      841 aatgccgagc ccgatggccc gacgcccgag cttgaggaag aataatga
//
