LOCUS       sequence-449313-         882 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression of the fusion of the NS2B-NS3 protease from 
            Dengue virus serotype 4 for biochemical assays.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 882)
  TITLE     ASAP Discovery Plasmids 2024
REFERENCE   2  (bases 1 to 882)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: DENV-4 strain H241 NS2B-NS3 protease fusion wi...
FEATURES             Location/Qualifiers
     source          1..882
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4..21
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             join(37..60,61..96,97..120)
                     /codon_start=1
                     /product="two Strep-Tag(R)II moieties connected by a linker
                     for enhanced binding to Strep-Tactin(R), an engineered form
                     of streptavidin (Schmidt et al., 2013)"
                     /label=Twin-Strep-tag
                     /translation="WSHPQFEKGGGSGGGSGGSAWSHPQFEK"
     CDS             145..165
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 atgcaccatc atcatcatca ttcttctggt gcgagctgga gtcacccaca attcgagaaa
       61 ggtggtggct ctggtggcgg ttcaggcggc tccgcctggt cccatccaca gtttgaaaag
      121 ggttctggtg tagatctggg taccgagaac ctgtacttcc aatccatggc ggatttatca
      181 ctggagaagg cggcgaatgt gcaatgggac gagatggcgg acataactgg ttcatctccg
      241 ataatcgagg taaagcagga cgaggacggc agtttcagta ttcgggatat tgaggaaacc
      301 aatatgggcg gtggtggtag tggtggtggc ggctcaggag cgttatggga cgtaccgtcc
      361 ccggctgcag cacaaaaggc gactctgacc gagggtgtgt accgtattat gcaacggggc
      421 cttttcggta agacgcaagt aggggttggc atacacatgg aaggggtttt ccatactatg
      481 tggcacgtga ctagaggttc cgtaatctgt cacgagacag ggcgactcga gccttcgtgg
      541 gctgacgtcc gaaatgacat gatcagttac ggcggtggat ggagacttgg tgacaaatgg
      601 gacaaggaag aggacgttca agtcttagcg attgagcctg ggaagaatcc aaagcatgtg
      661 caaacaaagc cgggattatt taagaccctg actggtgaaa ttggggcggt aacccttgac
      721 ttcaagcctg gtacaagtgg ttctcctatt atcaaccgaa aggggaaggt gatcggcctt
      781 tatggaaatg gcgttgttac aaagtcaggg gattacgtca gtgctatcac acaggctgaa
      841 cgcaccgggg agccggatta tgaggtagac gaggattaat ga
//
