LOCUS       sequence-449314-         861 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression of the fusion of the NS2B-NS3 protease from 
            Murray Valley encephalitis virus for biochemical assay.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 861)
  TITLE     ASAP Discovery Plasmids 2024
REFERENCE   2  (bases 1 to 861)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: MVEV strain MVE-1-51 NS2B-NS3 protease fusion ...
FEATURES             Location/Qualifiers
     source          1..861
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4..21
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             join(37..60,61..96,97..120)
                     /codon_start=1
                     /product="two Strep-Tag(R)II moieties connected by a linker
                     for enhanced binding to Strep-Tactin(R), an engineered form
                     of streptavidin (Schmidt et al., 2013)"
                     /label=Twin-Strep-tag
                     /translation="WSHPQFEKGGGSGGGSGGSAWSHPQFEK"
     CDS             145..165
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 atgcaccatc atcatcatca ttcttctggt gcgagctgga gtcacccaca attcgagaaa
       61 ggtggtggct ctggtggcgg ttcaggcggc tccgcctggt cccatccaca gtttgaaaag
      121 ggttctggtg tagatctggg taccgagaac ctgtacttcc aatccatggc cacagatatg
      181 tggctggagc gtgctgcaga cgtatcttgg gaggctgggg ctgcgataac aggtacatcg
      241 gaaagattgg acgtccagtt agacgatgat ggtgattttc acctgttgaa cgacccggga
      301 gtaccatggg caggaggagg cggcagtggt gggggggggg gtgtattttg ggacacgcct
      361 agtcctaaag tgtatccaaa gggagatacg accccgggcg tttatcgtat aatggcacga
      421 ggaattctcg gccgctatca ggccggggtg ggcgtaatgc atgaaggtgt atttcacacg
      481 ttgtggcaca caacacgtgg ggcagcgatt atgtcggggg agggtcgcct gactccgtat
      541 tggggcaatg tgaaagagga tcgcgttacg tatggtggcc cttggaagtt agaccagaaa
      601 tggaacggag tggatgacgt ccaaatgatt gtagtggaac cgggaaaacc agcgatcaac
      661 gtgcagacga aacctggaat ttttaaaaca gcccacggtg agatcggcgc ggtttcgctg
      721 gattatccaa taggtacctc tggatccccg attgttaact ccaatgggga aattattggc
      781 ctgtatggta atggtgtgat cttagggaat ggggcttatg tcagcgcaat agttcaaggt
      841 gagcgagttg aggagtaatg a
//
