LOCUS       sequence-449315-         861 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression of the fusion of the NS2B-NS3 protease from 
            Powassan virus for biochemical assay.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 861)
  TITLE     ASAP Discovery Plasmids 2024
REFERENCE   2  (bases 1 to 861)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: POWV strain LB NS2B-NS3 protease fusion with n...
FEATURES             Location/Qualifiers
     source          1..861
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4..21
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             join(37..60,61..96,97..120)
                     /codon_start=1
                     /product="two Strep-Tag(R)II moieties connected by a linker
                     for enhanced binding to Strep-Tactin(R), an engineered form
                     of streptavidin (Schmidt et al., 2013)"
                     /label=Twin-Strep-tag
                     /translation="WSHPQFEKGGGSGGGSGGSAWSHPQFEK"
     CDS             145..165
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 atgcaccatc atcatcatca ttcttctggt gcgagctgga gtcacccaca attcgagaaa
       61 ggtggtggct ctggtggcgg ttcaggcggc tccgcctggt cccatccaca gtttgaaaag
      121 ggttctggtg tagatctggg taccgagaac ctgtacttcc aatccatgca gttgactgcg
      181 gaatgggcag gtgtcgtgga atggaacccc gaattagtta atgaaggtgg tgaggtttcc
      241 ctcaaggttc gtcaggatgc catgggcaat ttacatctta cagaggtgga gcgcgaggaa
      301 gcggcccttg gagggggtgg atccgggggt ggcggcgggc tcgtattcag cgggcaactt
      361 ccagatcaag gggagaagcg atcgtttgat ataaaggagg gcgtgtaccg catctatgcc
      421 ccgggactgt tttggggcta tcgtcaaatc ggcgtgggat acggaacaaa aggcgttctg
      481 cacaccatgt ggcatgtaac acgtggtgcg gccctgagtg ttgaaggggc aacgtctggg
      541 ccctactggg ctgatgtacg ggaagacgtt gtgtgctatg gaggcgcgtg gggtctggat
      601 aaaaaatggg ggggcgaggt ggttcaggtt catgcgttcc cgcctgattc ggggcataaa
      661 atacatcaat gtcaaccggg gaaattgaac ttagaaggtg gccgcgtgtt aggtgcgatc
      721 cccattgact taccgcgggg caccagtgga agcccaatta ttaatgctca aggcgatgtc
      781 cttggcctgt atgggaatgg cttaaaatcc aacgacgttt atatatcctc aattgcacaa
      841 ggtaacgtcg aaaagtaatg a
//
