LOCUS       sequence-449316-         867 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression of the fusion of the NS2B-NS3 protease from 
            Rocio virus for biochemical assay.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 867)
  TITLE     ASAP Discovery Plasmids 2024
REFERENCE   2  (bases 1 to 867)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: ROCV strain SPH 34675 NS2B-NS3 protease fusion...
FEATURES             Location/Qualifiers
     source          1..867
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4..21
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             join(37..60,61..96,97..120)
                     /codon_start=1
                     /product="two Strep-Tag(R)II moieties connected by a linker
                     for enhanced binding to Strep-Tactin(R), an engineered form
                     of streptavidin (Schmidt et al., 2013)"
                     /label=Twin-Strep-tag
                     /translation="WSHPQFEKGGGSGGGSGGSAWSHPQFEK"
     CDS             145..165
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 atgcaccatc atcatcatca ttcttctggt gcgagctgga gtcacccaca attcgagaaa
       61 ggtggtggct ctggtggcgg ttcaggcggc tccgcctggt cccatccaca gtttgaaaag
      121 ggttctggtg tagatctggg taccgagaac ctgtacttcc aatccatgag tacggacatg
      181 tggatcgagc gttgtgcaga tgtgacgtgg gaggaagagg cagagattac aggcacctcc
      241 ccgcgtctgg atgtggaact tgacgataat ggtgacttca aaatgatcaa cgatccggga
      301 gttccaatgt gggctggtgg cggaggcagc gggggcggtg ggggcgttct ttgggacctg
      361 ccggcgccca aacaaatggg ccgttcagat atgaaaccag gtgtatatcg tgttatgaca
      421 tctggtgttt taggttcgta tcagtcaggg gttggcgtga tgtacgatgg agtctttcat
      481 accatgtggc acgttaccca gggtgcagca ctgcgcaacg gtgaggggcg actgaatcct
      541 acatggggct cggttcgcga tgatctgatc acttatggcg gaaaatggaa acttagtgca
      601 acttgggatg ggacagagga ggttcagctt attgccgctg agccgggcaa acccgtcaag
      661 aactttcaga cccgtccggg tgtttttaag acgccagccg gggaagttgg agccatcacc
      721 ctggactttc cgaaaggtac ctcgggctcg cccatcgtga ataaagctgg tgctgtcatc
      781 gggttgtatg gcaatggcct cgttctgtct catggagcgt acgtatcagc tataagccag
      841 ggcgaacgcc aggaagaaga ataatga
//
