LOCUS       sequence-449317-         885 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression of the fusion of the NS2B-NS3 protease from 
            Spondweni virus for biochemical assay.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 885)
  TITLE     ASAP Discovery Plasmids 2024
REFERENCE   2  (bases 1 to 885)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: SPOV strain SM-6 V-1 NS2B-NS3 protease fusion ...
FEATURES             Location/Qualifiers
     source          1..885
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4..21
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             join(37..60,61..96,97..120)
                     /codon_start=1
                     /product="two Strep-Tag(R)II moieties connected by a linker
                     for enhanced binding to Strep-Tactin(R), an engineered form
                     of streptavidin (Schmidt et al., 2013)"
                     /label=Twin-Strep-tag
                     /translation="WSHPQFEKGGGSGGGSGGSAWSHPQFEK"
     CDS             145..165
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 atgcaccatc atcatcatca ttcttctggt gcgagctgga gtcacccaca attcgagaaa
       61 ggtggtggct ctggtggcgg ttcaggcggc tccgcctggt cccatccaca gtttgaaaag
      121 ggttctggtg tagatctggg taccgagaac ctgtacttcc aatccatgtc ggggaagtcg
      181 gttgatatgt atatcgaaaa ggtctgtgac atatcatggg acaaggacgc agagatcacg
      241 gggacgtcac ctcgccttga cgtagccttg gatgacagtg gagacttctc tcttatccaa
      301 gatgatggcc caccaacgcg cgagggcggc ggtggcagcg gtggtggtgg tggtgcaatg
      361 tgggacattc ctagcccgag agaggtgaag aagggtgaaa caaccgctgg cgtctatcgt
      421 attatgacac gtaagctgtt aggttcaacc caagtagggg ctggagtcat gcatgagggt
      481 gtcttccaca cgatgtggca cgtaacaaag gggtccgcac tccgctcggg cgagggacgt
      541 ctggatccgt attggggcaa cgtaaagcaa gatctgatct catactgcgg accgtggaag
      601 ttggacggga aatgggacgg cgttagcgag gtacaactga tagctgtagc ccctggcgag
      661 cgtgcccgta atgtccaaac caaaccaggt gtgtttaaga cgaccgatgg tgagatcgga
      721 gcccttgcct tagattttcc gggcggatcg agtgggagtc caattatcga caagaacggg
      781 catgttatcg gcttatacgg taacggggtc gttgttaaaa gtggttctta tgtaagtgcg
      841 ataatgcaaa cggagaagat ggaagagccc gcagtggatt aatga
//
