LOCUS       sequence-449318-         864 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression of the fusion of the NS2B-NS3 protease from St.
            Louis encephalitis virus for biochemical assay.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 864)
  TITLE     ASAP Discovery Plasmids 2024
REFERENCE   2  (bases 1 to 864)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: SLEV strain Kern217 NS2B-NS3 protease fusion w...
FEATURES             Location/Qualifiers
     source          1..864
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4..21
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             join(37..60,61..96,97..120)
                     /codon_start=1
                     /product="two Strep-Tag(R)II moieties connected by a linker
                     for enhanced binding to Strep-Tactin(R), an engineered form
                     of streptavidin (Schmidt et al., 2013)"
                     /label=Twin-Strep-tag
                     /translation="WSHPQFEKGGGSGGGSGGSAWSHPQFEK"
     CDS             145..165
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 atgcaccatc atcatcatca ttcttctggt gcgagctgga gtcacccaca attcgagaaa
       61 ggtggtggct ctggtggcgg ttcaggcggc tccgcctggt cccatccaca gtttgaaaag
      121 ggttctggtg tagatctggg taccgagaac ctgtacttcc aatccatgag tggtaaagcc
      181 gctgaaatgt ggattgaaaa ggcggcagat attacttggg aacagaatgc agagataacg
      241 ggcacctctc cgagactcga tgtagacctg gacagccacg gcaactttaa actgctgaat
      301 gatcctggtg ctccaggggg tggtgggtct gggggcggag ggggcgcact ctgggatgtt
      361 ccctctccta aagtatatcc taagtgcgaa actaaacccg gtatttaccg tattatgact
      421 cgcgggatct taggaacgtt ccaagccggt gtaggagtta tgcacgaggg agttttccac
      481 accatgtggc acgcgacgga aggtgccgtc ttacggaatg gagaaggtcg actggatcct
      541 tacgcgggag acgtacggaa tgatctgatt tcttatgggg ggccttggaa gctgagcgct
      601 acttgggacg gcacagagga agtccagatg attgccgtgg ctcccggcaa gccggcgata
      661 aatgttcaaa ctacgcccgg ggtattcaag acacctctgg gcacaatcgg cgctgttact
      721 ttagattttc cgaaagggac ctcgggatca cccataatca ataaaaaagg ggaaattata
      781 gggctgtacg gaaatggcgt ccttatcggc cagggggagt acgtgtcggg aattattcag
      841 ggagaacgca ccgaggaata atga
//
