LOCUS       sequence-449319-         861 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression of the fusion of the NS2B-NS3 protease from 
            Usutu virus for biochemical assay.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 861)
  TITLE     ASAP Discovery Plasmids 2024
REFERENCE   2  (bases 1 to 861)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: USUV strain Vienna 2001 NS2B-NS3 protease fusi...
FEATURES             Location/Qualifiers
     source          1..861
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4..21
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             join(37..60,61..96,97..120)
                     /codon_start=1
                     /product="two Strep-Tag(R)II moieties connected by a linker
                     for enhanced binding to Strep-Tactin(R), an engineered form
                     of streptavidin (Schmidt et al., 2013)"
                     /label=Twin-Strep-tag
                     /translation="WSHPQFEKGGGSGGGSGGSAWSHPQFEK"
     CDS             145..165
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 atgcaccatc atcatcatca ttcttctggt gcgagctgga gtcacccaca attcgagaaa
       61 ggtggtggct ctggtggcgg ttcaggcggc tccgcctggt cccatccaca gtttgaaaag
      121 ggttctggtg tagatctggg taccgagaac ctgtacttcc aatccatgag tacagatctg
      181 tggttggaaa gagccgcaga tatcacttgg gagacagacg ccgcaataac aggaacttcc
      241 caacgtctgg atgtcaaatt ggatgatgac ggtgatttcc atttaattaa cgatccgggg
      301 gtgccgtggg cagggggcgg cggctctgga ggaggtggag gcgtattttg ggacacccca
      361 gcaccacgaa catatccgaa gggcgataca tcaccgggcg tttatcgcat catgagccgg
      421 tacatcctgg gaacttatca ggccggcgtc ggggtgatgt atgaaggcgt tcttcacact
      481 ttatggcata caacacgagg ggctgcgata agaagcggcg agggccggct tacaccttac
      541 tgggggagcg taaaagaaga tcgcatcacg tacgggggtc cgtggaagtt tgatcgtaaa
      601 tggaacggtt tagacgacgt gcaacttata atagttgcgc cggggaaagc tgcgataaac
      661 atacagacga aaccaggcat ttttaagact ccacaaggtg agattggagc agttagcctg
      721 gattaccctg aaggaacttc aggatctcca atcttagata aaaatggaga cattgtagga
      781 ctctatggta atggagtcat tttaggcaat ggtagctacg ttagcgcaat tgtccagggc
      841 gagcgtgaag aggagtaatg a
//
