LOCUS       sequence-449320-         876 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression of the fusion of the NS2B-NS3 protease from 
            Wesselsbron virus for biochemical assay.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 876)
  TITLE     ASAP Discovery Plasmids 2024
REFERENCE   2  (bases 1 to 876)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: WESSV strain SAH177 NS2B-NS3 protease fusion w...
FEATURES             Location/Qualifiers
     source          1..876
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4..21
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             join(37..60,61..96,97..120)
                     /codon_start=1
                     /product="two Strep-Tag(R)II moieties connected by a linker
                     for enhanced binding to Strep-Tactin(R), an engineered form
                     of streptavidin (Schmidt et al., 2013)"
                     /label=Twin-Strep-tag
                     /translation="WSHPQFEKGGGSGGGSGGSAWSHPQFEK"
     CDS             145..165
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 atgcaccatc atcatcatca ttcttctggt gcgagctgga gtcacccaca attcgagaaa
       61 ggtggtggct ctggtggcgg ttcaggcggc tccgcctggt cccatccaca gtttgaaaag
      121 ggttctggtg tagatctggg taccgagaac ctgtacttcc aatccatgag cggcaaggtg
      181 gatgggttag taattaagaa agtagcagac gttacatggg atgaagatgc cgaaatatcc
      241 ggggcatctc atcgctatga tgttgaacag accgatacgg gtgagtttaa actgcgaaac
      301 gaagagccag caccgtgggc gggcggaggg ggctcgggcg gaggaggagg tgtgttatgg
      361 gacattccaa cggtagttcc gccggaggaa gtatcctatt tagaggatgg cgtgtacacc
      421 atcaatcaga attcttttct gggccttgcg cagaaaggtg ttggtgtggt caaagatggt
      481 gtatttcata ctatgtggca cgtgacgcgc ggggcattcc tgctgcatgc cggcaaaaga
      541 atgaccccta gttgggccaa tgtgaaagaa gatctgattt catatggcgg cggatggaaa
      601 ttggatgcta aatgggacgg ttcagaagag gttcagttga ttgcagtgag tcccggaaag
      661 gtcccggtga acgttcagac tacaccatct gtttttcaat taaagaatgg gaaagaaatt
      721 ggggcggtta atttggacta cccgtccgga acaagtggct cacccatctt aaataaaaat
      781 ggcgatgtta tcggcctgta cggcaacggc atactgattg gtaacaatac ttacgtctcc
      841 gcaattgcgc aatcggatag cgtagaggaa taatga
//
