LOCUS       sequence-449321-         900 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression of the fusion of the NS2B-NS3 protease from 
            West Nile virus with an H163A - catalytically inactive mutation for 
            xxxxx.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 900)
  TITLE     ASAP Discovery Plasmids 2024
REFERENCE   2  (bases 1 to 900)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: WNV strain-NY99 NS2B-NS3 protease fusion with ...
FEATURES             Location/Qualifiers
     source          1..900
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4..21
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             join(37..60,61..96,97..120)
                     /codon_start=1
                     /product="two Strep-Tag(R)II moieties connected by a linker
                     for enhanced binding to Strep-Tactin(R), an engineered form
                     of streptavidin (Schmidt et al., 2013)"
                     /label=Twin-Strep-tag
                     /translation="WSHPQFEKGGGSGGGSGGSAWSHPQFEK"
     CDS             145..165
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 atgcaccatc atcatcatca ttcttctggt gcgagctgga gtcacccaca attcgagaaa
       61 ggtggtggct ctggtggcgg ttcaggcggc tccgcctggt cccatccaca gtttgaaaag
      121 ggttctggtg tagatctggg taccgagaac ctgtacttcc aatccatgag cactgatatg
      181 tggattgaac gcacggcaga catcagctgg gagtcggatg ctgagatcac aggttcaagc
      241 gaaagagtgg atgtacgatt agacgatgac ggtaattttc aactgatgaa tgaccctggc
      301 gcgccgtggg caggcggcgg cggctctggt ggaggaggtg gcgttttgtg ggacacgccg
      361 tcccccaaag aatacaagaa gggtgacacg actaccggtg tttacagaat tatgacccgg
      421 ggtttgcttg ggtcgtacca ggccggggca ggggtgatgg tagagggagt tttccatacc
      481 ttatggcaca ctaccaaagg tgccgcactt atgtctggtg agggtcgcct tgatccttat
      541 tggggtagcg taaaagagga tcggctctgt tacggcggac catggaagct tcaacacaag
      601 tggaacggac aggatgaagt tcagatgatt gtcgtagagc cgggcaagaa tgtcaagaac
      661 gtgcaaacaa agcccggtgt attcaaaacg ccggaaggcg aaattggcgc tgtgaccctt
      721 gactttccaa ctgggaccag cggctcacca atagtcgaca agaatggaga tgtcattggg
      781 ttgtacggaa atggtgttat tatgcccaat ggctcgtaca tctccgctat cgtacaagga
      841 gagcgcatgg acgaaccgat acctgcaggc ttcgaacctg agatgttaag aaagaagtaa
//
