LOCUS       sequence-449322-         891 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression of the fusion of the NS2B-NS3 protease from 
            Zika virus for biochemical assay.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 891)
  TITLE     ASAP Discovery Plasmids 2024
REFERENCE   2  (bases 1 to 891)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: ZIKV isolate VE_Ganxian2016 NS2B-NS3 protease ...
FEATURES             Location/Qualifiers
     source          1..891
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4..21
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             join(37..60,61..96,97..120)
                     /codon_start=1
                     /product="two Strep-Tag(R)II moieties connected by a linker
                     for enhanced binding to Strep-Tactin(R), an engineered form
                     of streptavidin (Schmidt et al., 2013)"
                     /label=Twin-Strep-tag
                     /translation="WSHPQFEKGGGSGGGSGGSAWSHPQFEK"
     CDS             145..165
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 atgcaccatc atcatcatca ttcttctggt gcgagctgga gtcacccaca attcgagaaa
       61 ggtggtggct ctggtggcgg ttcaggcggc tccgcctggt cccatccaca gtttgaaaag
      121 ggttctggtg tagatctggg taccgagaac ctgtacttcc aatccatgag tggaaagtct
      181 gtcgatatgt acatcgagcg agccggcgac atcacatggg agaaagacgc agaggttacc
      241 gggaatagcc cgcggcttga cgttgcgttg gacgagagtg gtgacttctc tctggtcgag
      301 gacgacggac cacctatgcg tgagggagga ggtgggtcag gtggtggtgg tggctcgggt
      361 gctctttggg atgtccctgc ccccaaagag gtgaagaagg gtgagacgac cgacggagtg
      421 tatcgcgtta tgacaagacg tttattaggg tcaacacaag taggtgttgg tgtaatgcaa
      481 gagggcgttt tccacacaat gtggcacgtt actaagggct cagctcttcg gtcaggtgag
      541 ggacgccttg acccgtactg gggcgacgtt aagcaagatc tggtgtctta ttgtggtccc
      601 tggaagttag acgctgcatg ggacggtcat agcgaggtcc aattgctggc agtgcccccg
      661 ggtgagagag cgcgaaatat tcaaacgctt cctgggattt tcaagaccaa ggacggtgac
      721 atcggtgcag tggcgctgga ttacccggcg ggaacgtccg gcagtccgat tttggataag
      781 tgcggtcggg taatcggact gtatggtaat ggggtggtga ttaagaacgg ctcatacgtg
      841 tctgcaataa cccagggccg tcgagaggaa gaaactcctg ttgagtaatg a
//
