LOCUS       sequence-449324-         855 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial co-expression of the NS2B and NS3 proteases from Zika 
            virus for crystallization assays in P43 2 2 (PDB:8PN6).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 855)
  TITLE     ASAP Discovery Plasmids 2024
REFERENCE   2  (bases 1 to 855)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: ZIKV isolate VE_Ganxian2016 NS2B-NS3 protease
            ...
FEATURES             Location/Qualifiers
     source          1..855
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4..21
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             28..681
                     /codon_start=1
                     /product="glutathione S-transferase from Schistosoma 
                     japonicum"
                     /label=GST
                     /translation="MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKK
                     FELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRY
                     GVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVL
                     YMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK"
     CDS             697..717
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 atgcaccatc accaccatca ctcatcgatg tctcccattt tgggttattg gaagataaag
       61 gggttagtac aaccgaccag attactgtta gagtaccttg aagagaagta tgaagagcat
      121 ttatatgagc gcgacgaagg cgataagtgg cgcaacaaga aatttgagtt aggcctcgag
      181 tttccgaacc ttccttatta tatcgacggg gacgtaaagt tgacgcaatc tatggcaata
      241 attcggtata tagcagacaa gcataacatg ttgggtggct gccccaaaga gcgtgccgag
      301 atctccatgt tagaaggagc cgtcctggac attcgctatg gtgtgtcgcg cattgcctac
      361 tcaaaagatt tcgagacttt aaaagttgac tttctgagca agctgccgga gatgcttaag
      421 atgttcgaag atcggctgtg tcacaagacg tacctcaacg gtgaccacgt tacccaccca
      481 gactttatgc tttacgatgc gctggacgtt gtgctttaca tggacccgat gtgtctggac
      541 gcgtttccga agttggtatg tttcaagaag cggattgagg ctattcccca aatagacaaa
      601 taccttaaga gtagtaagta catcgcgtgg ccacttcaag gttggcaagc taccttcggc
      661 ggcggcgacc atcccccaaa gtcgggcggc ggcagcgaga acctgtactt ccaatccatg
      721 ggaaagtcag ttgatatgta catagaacgg gctggagata ttacctggga aaaagacgca
      781 gaagtgacgg gcaacagccc gcggttggat gttgctttag acgaatctgg agattttagc
      841 ttagttgaag aataa
//
