LOCUS       sequence-449325-        2028 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression of the Zika virus NS3 helicase.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2028)
  TITLE     ASAP Discovery Plasmids 2024
REFERENCE   2  (bases 1 to 2028)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: ZIKV isolate PRVABC59 NS3 helicase domain
FEATURES             Location/Qualifiers
     source          1..2028
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4..21
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             28..681
                     /codon_start=1
                     /product="glutathione S-transferase from Schistosoma 
                     japonicum"
                     /label=GST
                     /translation="MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKK
                     FELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRY
                     GVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVL
                     YMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK"
     CDS             697..717
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 atgcaccatc accaccatca ctcatcgatg tctcccattt tgggttattg gaagataaag
       61 gggttagtac aaccgaccag attactgtta gagtaccttg aagagaagta tgaagagcat
      121 ttatatgagc gcgacgaagg cgataagtgg cgcaacaaga aatttgagtt aggcctcgag
      181 tttccgaacc ttccttatta tatcgacggg gacgtaaagt tgacgcaatc tatggcaata
      241 attcggtata tagcagacaa gcataacatg ttgggtggct gccccaaaga gcgtgccgag
      301 atctccatgt tagaaggagc cgtcctggac attcgctatg gtgtgtcgcg cattgcctac
      361 tcaaaagatt tcgagacttt aaaagttgac tttctgagca agctgccgga gatgcttaag
      421 atgttcgaag atcggctgtg tcacaagacg tacctcaacg gtgaccacgt tacccaccca
      481 gactttatgc tttacgatgc gctggacgtt gtgctttaca tggacccgat gtgtctggac
      541 gcgtttccga agttggtatg tttcaagaag cggattgagg ctattcccca aatagacaaa
      601 taccttaaga gtagtaagta catcgcgtgg ccacttcaag gttggcaagc taccttcggc
      661 ggcggcgacc atcccccaaa gtcgggcggc ggcagcgaga acctgtactt ccaatccatg
      721 ctcaagaaga aacaattgac cgtgcttgac ttgcaccctg gcgcgggcaa gactcgccgt
      781 gtgctgccgg agattgtaag agaggctatc aagaaacgcc tgcgtaccgt aattctcgct
      841 ccgacgcgtg tggtagccgc agaaatggaa gaagcgctgc gcggcctgcc tgtacgatat
      901 atgactactg ctgttaatgt gactcacagt ggtacggaga ttgtggactt aatgtgtcac
      961 gcgacgttta cgagtcgtct gttgcagccg attcgcgttc caaactataa cctgaatatt
     1021 atggacgagg ctcattttac cgatcctagt tctattgcag ctcgcggtta tatcagcact
     1081 agagtggaga tgggagaagc cgccgctata ttcatgacag caactccccc gggtacacgc
     1141 gacgccttcc ccgatagcaa ttctcctata atggatactg aggtagaggt tcccgaacgc
     1201 gcgtggtcga gtgggttcga ctgggtaacc gatcactccg gaaagaccgt gtggtttgtc
     1261 ccgagtgttc ggaatggtaa cgagattgcc gcgtgcttaa ccaaagcggg taaaagagta
     1321 attcaattgt ctcgaaagac gttcgaaacc gagttccaaa agaccaagaa ccaggaatgg
     1381 gatttcgtta tcactacgga tataagcgaa atgggagcaa attttaaagc ggatcgtgta
     1441 attgattcac gccgctgtct gaagcctgtg attttggacg gcgaacgcgt aattctggcc
     1501 ggtccaatgc cggtaaccca cgcatctgcg gcacaacgtc gcggtcgaat tgggcgcaat
     1561 ccgaataagc caggtgacga atatatgtac ggtggcggct gcgctgaaac cgacgagggt
     1621 cacgcccatt ggttagaggc ccgtatgtta ctcgataata tttatctgca agacgggctg
     1681 attgcaagcc tgtaccgccc cgaagcggac aaagtcgcag ctatcgaagg cgaattcaaa
     1741 cttcgtacgg aacagcgtaa aacatttgtc gagttaatga aacgtggcga tctgccggtt
     1801 tggctcgcgt accaagtagc gtccgcgggt atcacgtata ccgatcgtcg atggtgtttc
     1861 gacggtacga caaataatac aatcatggaa gattcagttc cggctgaagt atggactaaa
     1921 tacggtgaga aacgggttct taagcctcgc tggatggacg cgcgagtgtg ctcggatcac
     1981 gccgcgttga aatcattcaa ggagtttgca gcaggtaagc gataatga
//
