LOCUS       sequence-449339-        9112 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Tet-inducible AAV shRNA vector to test efficacy of shRNAs. Used in 
            combination with pGFPns-reporter (Addgene plasmid #35626)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9112)
  AUTHORS   Naughton BJ, Han DD, Gu HH
  TITLE     Fluorescence-based evaluation of shRNA efficacy.
  JOURNAL   Anal Biochem. 2011 Oct 1;417(1):162-4. Epub 2011 Jun 13.
  PUBMED    21726522
REFERENCE   2  (bases 1 to 9112)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Anal 
            Biochem."; date: "2011-10-1"; volume: "417(1)"; pages: "162-4. Epub 
            2011 Jun 13"
COMMENT     Sequence Label: pAAV-Ptet-RFP-shR-rtTA
FEATURES             Location/Qualifiers
     source          1..9112
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    54..72
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    89..107
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    125..143
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    161..179
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    196..214
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    232..250
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        262..300
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     297..321
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             337..1032
                     /codon_start=1
                     /product="red fluorescent protein from Entacmaea 
                     quadricolor"
                     /label=TurboRFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMKIKV
                     VEGGPLPFAFDILATSFMYGSKAFINHTQGIPDFFKQSFPEGFTWERITTYEDGGVLTA
                     TQDTSFQNGCIIYNVKINGVNFPSNGPVMQKKTRGWEANTEMLYPADGGLRGHSQMALK
                     LVGGGYLHCSFKTTYRSKKPAKNLKMPGFHFVDHRLERIKEADKETYVEQHEMAVAKYC
                     DLPSKLGHR"
     ncRNA           1045..1138
                     /label=5' miR-30a
                     /note="sequence upstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           2437..2563
                     /label=3' miR-30a
                     /note="sequence downstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     promoter        2689..3900
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     3737..3756
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     protein_bind    complement(3913..3946)
                     /label=loxP511
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATAC) (Shaw et al., 2021). loxP511 sites are 
                     compatible with each other, but incompatible with wild-type
                     loxP sites."
     CDS             3953..4660
                     /codon_start=1
                     /product="reverse tetracycline transactivator 3"
                     /label=rtTA3
                     /note="activates expression from Tet-responsive promoters 
                     in the presence of doxycycline"
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPG"
     protein_bind    complement(4661..4694)
                     /label=loxP511
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATAC) (Shaw et al., 2021). loxP511 sites are 
                     compatible with each other, but incompatible with wild-type
                     loxP sites."
     misc_feature    4713..5301
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4766..4786)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5184..5195)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    5334..5558
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      complement(5988..6576)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6068..6087)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6747..7538,7539..7607))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7370..7389
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7608..7712)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     7780..7798
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(7836..7858)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     7958..7977
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8191..8210
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     polyA_signal    8277..8501
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     promoter        8527..8545
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     promoter        8931..8949
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     repeat_region   8970..9099
                     /label=AAV2 ITR
ORIGIN
        1 taacccgcca tgctacttat ctacgtagcc atgctctagg tacccgagtt tactccctat
       61 cagtgataga gaacgatgtc gagtttactc cctatcagtg atagagaacg tatgtcgagt
      121 ttactcccta tcagtgatag agaacgtatg tcgagtttac tccctatcag tgatagagaa
      181 cgtatgtcga gtttatccct atcagtgata gagaacgtat gtcgagttta ctccctatca
      241 gtgatagaga acgtatgtcg aggtaggcgt gtacggtggg aggcctatat aagcagagct
      301 cgtttagtga accgtcagat cgcaccggtc gccaccatga gcgagctgat caaggagaac
      361 atgcacatga agctgtacat ggagggcacc gtgaacaacc accacttcaa gtgcacatcc
      421 gagggcgaag gcaagcccta cgagggcacc cagaccatga agatcaaggt ggtcgagggc
      481 ggccctctcc ccttcgcctt cgacatcctg gctaccagct tcatgtacgg cagcaaagcc
      541 ttcatcaacc acacccaggg catccccgac ttctttaagc agtccttccc tgagggcttc
      601 acatgggaga gaatcaccac atacgaagac gggggcgtgc tgaccgctac ccaggacacc
      661 agcttccaga acggctgcat catctacaac gtcaagatca acggggtgaa cttcccatcc
      721 aacggccctg tgatgcagaa gaaaacacgc ggctgggagg ccaacaccga gatgctgtac
      781 cccgctgacg gcggcctgag aggccacagc cagatggccc tgaagctcgt gggcgggggc
      841 tacctgcact gctccttcaa gaccacatac agatccaaga aacccgctaa gaacctcaag
      901 atgcccggct tccacttcgt ggaccacaga ctggaaagaa tcaaggaggc cgacaaagag
      961 acctacgtcg agcagcacga gatggctgtg gccaagtact gcgacctccc tagcaaactg
     1021 gggcacagat aatcgatagt ttgtttgaat gaggcttcag tactttacag aatcgttgcc
     1081 tgcacatctt ggaaacactt gctgggatta cttcttcagg ttaacccaac agaaggctcg
     1141 aggtatattg ctgttgacag tgagcgtgaa gagccacagt gtggaagatc tgccctttct
     1201 tcaaaggagt gggctatgct gtgatcctca ttgccctcta tgtcggcttt tactacaatg
     1261 tcatcatcgc ctggtcactc tactacctct ttgcaagctt caccttgaac ctgccctgga
     1321 ccaactgcgg acactcctgg aacagcccca actgtacgga ccccaaactc ctcaacgcct
     1381 cggtgctggg tgaccatacc aaatactcca aatacaagtt cacaccagct gcggagtttt
     1441 atgaacgcgg agtcctgcac ttgcacgaga gcagtgggat ccatgacatc ggcctgcctc
     1501 aatggcagct gctgctctgc ctgatggtcg tcatcgttgt cttgtatttc agcctctgga
     1561 aaggagtgaa gacatcggga aaggttgtct ggatcacagc caccctgcct tactttgtct
     1621 tgtttgtgct cctggtccat ggcgtcacac tgcccggtgc ctccaatggc atcaatgcct
     1681 acttgcacat tgacttctac cgcctcaagg aggccacggt atggattgat gctgcaaccc
     1741 agatattttt ttctctgggg gctggatttg gagtcttgat tgcatttgcc agttacaata
     1801 aatttgacaa caactgttac agggatgctc tactgaccag caccatcaac tgtgttacca
     1861 gttttatttc tggatttgcc atcttctcca tccttggtta tatggcccat gaacataagg
     1921 tcaatattga ggatgttgcc actgaaggag ctgggcttgt gtttatcctg tatccagaag
     1981 ccatctctac tctgtcagga tccacgttct gggccgtcct gttcttcttg atgctcctgg
     2041 ctctggggct agatagttca atgggaggca tggaagcagt catcactgga ctggctgatg
     2101 acttccaggt cctgaagcga caccggaaac tcttcacgtg tgttgtcacc atcagcacct
     2161 tccttctggc attgttttgc ataacaaagg gagggattta tgtcctcact ctgctggaca
     2221 cctttgcagc aggcacctcc attctgtttg cggtgcttat ggaagctatt ggagtttcct
     2281 ggttttatgg tgtggacagg ttcagcaatg acatccagca gatgatgggg tttaagccag
     2341 gcctgtactg gaggctgtgt tggaagttcg tgagccccgc cttcctcctg tttgtggtgg
     2401 tggtcagcat catcaacttc aagccgctct tcatgcctac tgcctcggaa ttcaaggggc
     2461 tactttagga gcaattatct tgtttactaa aactgaatac cttgctatct ctttgataca
     2521 tttttacaaa gctgaattaa aatggtataa attaaatcac ttttttcaat tggaagacta
     2581 atgcggccgg ccaccgggtc tgcttttgtt gacgtctttc cttgtttgac ttgacatggt
     2641 cggttggtca tccgggccct atagtgagtc gtcttaccta ggacgcgtgg cctccgcgcc
     2701 gggttttggc gcctcccgcg ggcgcccccc tcctcacggc gagcgctgcc acgtcagacg
     2761 aagggcgcag cgagcgtcct gatccttccg cccggacgct caggacagcg gcccgctgct
     2821 cataagactc ggccttagaa ccccagtatc agcagaagga cattttagga cgggacttgg
     2881 gtgactctag ggcactggtt ttctttccag agagcggaac aggcgaggaa aagtagtccc
     2941 ttctcggcga ttctgcggag ggatctccgt ggggcggtga acgccgatga ttatataagg
     3001 acgcgccggg tgtggcacag ctagttccgt cgcagccggg atttgggtcg cggttcttgt
     3061 ttgtggatcg ctgtgatcgt cacttggtga gtagcgggct gctgggctgg ccggggcttt
     3121 cgtggccgcc gggccgctcg gtgggacgga agcgtgtgga gagaccgcca agggctgtag
     3181 tctgggtccg cgagcaaggt tgccctgaac tgggggttgg ggggagcgca gcaaaatggc
     3241 ggctgttccc gagtcttgaa tggaagacgc ttgtgaggcg ggctgtgagg tcgttgaaac
     3301 aaggtggggg gcatggtggg cggcaagaac ccaaggtctt gaggccttcg ctaatgcggg
     3361 aaagctctta ttcgggtgag atgggctggg gcaccatctg gggaccctga cgtgaagttt
     3421 gtcactgact ggagaactcg gtttgtcgtc tgttgcgggg gcggcagtta tggcggtgcc
     3481 gttgggcagt gcacccgtac ctttgggagc gcgcgccctc gtcgtgtcgt gacgtcaccc
     3541 gttctgttgg cttataatgc agggtggggc cacctgccgg taggtgtgcg gtaggctttt
     3601 ctccgtcgca ggacgcaggg ttcgggccta gggtaggctc tcctgaatcg acaggcgccg
     3661 gacctctggt gaggggaggg ataagtgagg cgtcagtttc tttggtcggt tttatgtacc
     3721 tatcttctta agtagctgaa gctccggttt tgaactatgc gctcggggtt ggcgagtgtg
     3781 ttttgtgaag ttttttaggc accttttgaa atgtaatcat ttgggtcaat atgtaatttt
     3841 cagtgttaga ctagtaaatt gtccgctaaa ttctggccgt ttttggcttt tttgttagac
     3901 gctagcggat ccataacttc gtatagtata cattatacga agttatgcca ccatgtctag
     3961 gctggacaag agcaaagtca taaacggagc tctggaatta ctcaatggtg tcggtatcga
     4021 aggcctgacg acaaggaaac tcgctcaaaa gctgggagtt gagcagccta ccctgtactg
     4081 gcacgtgaag aacaagcggg ccctgctcga tgccctgcca atcgagatgc tggacaggca
     4141 tcatacccac ttctgccccc tggaaggcga gtcatggcaa gactttctgc ggaacaacgc
     4201 caagtcatac cgctgtgctc tcctctcaca tcgcgacggg gctaaagtgc atctcggcac
     4261 ccgcccaaca gagaaacagt acgaaaccct ggaaaatcag ctcgcgttcc tgtgtcagca
     4321 aggcttctcc ctggagaacg cactgtacgc tctgtccgcc gtgggccact ttacactggg
     4381 ctgcgtattg gaggaacagg agcatcaagt agcaaaagag gaaagagaga cacctaccac
     4441 cgattctatg cccccacttc tgagacaagc aattgagctg ttcgaccggc agggagccga
     4501 acctgccttc cttttcggcc tggaactaat catatgtggc ctggagaaac agctaaagtg
     4561 cgaaagcggc gggccgaccg acgcccttga cgattttgac ttagacatgc tcccagccga
     4621 tgcccttgac gattttgacc ttgacatgct ccccgggtaa ataacttcgt atagtataca
     4681 ttatacgaag ttatggatcc aagcttatcg ataatcaacc tctggattac aaaatttgtg
     4741 aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt
     4801 taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata
     4861 aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg
     4921 tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc
     4981 tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct
     5041 gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt
     5101 cggggaaatc atcgtccttt ccttggctgc tcgcctgtgt tgccacctgg attctgcgcg
     5161 ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc
     5221 tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg agtcggatct
     5281 ccctttgggc cgcctccccg catcgatacc gtcgactcgc tgatcagcct cgactgtgcc
     5341 ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg
     5401 tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag
     5461 gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg attgggaaga
     5521 caatagcagg catgctgggg atgcggtggg ctctatggct tctgaggcgg aaagaaccag
     5581 ctggggctcg actagagcat ggctacgtag ataagtagca tggcgggtta atcattaact
     5641 acaaggaacc cctagtgatg gagttggcca ctccctctct gcgcgctcgc tcgctcactg
     5701 aggccgggcg accaaaggtc gcccgacgcc cgggcggcct cagtgagcga gcgagcgcgc
     5761 agagcttttt gcaaaagcct aggcctccaa aaaagcctcc tcactacttc tgcttcctcg
     5821 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
     5881 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
     5941 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
     6001 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
     6061 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
     6121 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
     6181 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
     6241 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
     6301 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
     6361 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
     6421 actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
     6481 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
     6541 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
     6601 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
     6661 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
     6721 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
     6781 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
     6841 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
     6901 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
     6961 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
     7021 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
     7081 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
     7141 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
     7201 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
     7261 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
     7321 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg
     7381 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
     7441 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
     7501 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
     7561 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
     7621 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
     7681 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac
     7741 gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc
     7801 tttcgtctcg cgcgtttcgg tgatgacggt gaaaacctct gacacatgca gctcccggag
     7861 acggtcacag cttgtctgta agcggatgcc gggagcagac aagcccgtca gggcgcgtca
     7921 gcgggtgttg gcgggtgtcg gggctggctt aactatgcgg catcagagca gattgtactg
     7981 agagtgcacc attcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag
     8041 taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc
     8101 caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag
     8161 cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac
     8221 cgcacctgtg gcgccggtgc ccctagagcc ccagctggtt ctttccgcct cagaagccat
     8281 agagcccacc gcatccccag catgcctgct attgtcttcc caatcctccc ccttgctgtc
     8341 ctgccccacc ccacccccca gaatagaatg acacctactc agacaatgcg atgcaatttc
     8401 ctcattttat taggaaagga cagtgggagt ggcaccttcc agggtcaagg aaggcacggg
     8461 ggaggggcaa acaacagatg gctggcaact agaaggcaca gtcgaggctg atcagcgagc
     8521 tctagcattt aggtgacact atagaatagg gccctctaga ctacacattg atcctagcag
     8581 aagcacaggc tgcagggtga cggtccatcc cgctctcctg ggcacaagac atgggcagcg
     8641 tgccatcatc ctgcgccgcc accgccggtg atgccggcca cgatgcgtcc ggcgtagagg
     8701 atctggctag cgatgaccct gctgattggt tcgctgacca tttccgggtg cgggacggcg
     8761 ttaccagaaa ctcagaaggt tcgtccaacc aaaccgactc tgacggcagt ttacgagaga
     8821 gatgataggg tctgcttcag taagccagat gctacacaat taggcttgta catattgtcg
     8881 ttagaacgcg gctacaatta atacataacc ttatgtatca tacacatacg atttaggtga
     8941 cactatagaa tacacggaat taattctagc tgcgcgctcg ctcgctcact gaggccgccc
     9001 gggcaaagcc cgggcgtcgg gcgacctttg gtcgcccggc ctcagtgagc gagcgagcgc
     9061 gcagagaggg agtggccaac tccatcacta ggggttcctt gtagttaatg at
//
