LOCUS       sequence-44934-e         850 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 850)
  TITLE     Stainier unpublished
REFERENCE   2  (bases 1 to 850)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: bActin-RSG-BiSKi
FEATURES             Location/Qualifiers
     source          1..850
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     intron          120..185
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             315..335
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    760..841
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(797..816)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 gagcgggccg agggccgcca ccacctgttc ctgtagctcg agggatcttt gtgaaggaac
       61 cttacttctg tggtgtgaca taattggaca aactacctac agagatttaa agctctaagg
      121 taaatataaa atttttaagt gtataatgtg ttaaactact gattctaatt gtttgtgtat
      181 tttagattcc aacctatgga actgatgaat gggagcagtg gtggaatgcc tttaatgagg
      241 aaaacctgtt ttgctcagaa gaaatgccat ctagtgatga tgaggctact gctgactctc
      301 aacattctac tcctccaaaa aagaagagaa aggtagaaga ccccaaggac tttccttcag
      361 aattgctaag ttttttgagt catgctgtgt ttagtaatag aactcttgct tgctttgcta
      421 tttacaccac aaaggaaaaa gctgcactgc tatacaagaa aattatggaa aaatattctg
      481 taacctttat aagtaggcat aacagttata atcataacat actgtttttt cttactccac
      541 acaggcatag agtgtctgct attaataact atgctcaaaa attgtgtacc tttagctttt
      601 taatttgtaa aggggttaat aaggaatatt tgatgtatag tgccttgact agagatcata
      661 atcagccata ccacatttgt agaggtttta cttgctttaa aaaacctccc acacctcccc
      721 ctgaacctga aacataaaat gaatgcaatt gttgttgtta acttgtttat tgcagcttat
      781 aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg
      841 cattctagtt
//
