LOCUS       sequence-449357-       10202 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Used to generate stable cell lines that express PERK(LD)-EGFP-HOTag3
            and to detect the unfolded proteins in the ER lumen..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10202)
  AUTHORS   Xu F, Wang L
  TITLE     Deciphering ER stress-unfolded protein response relationship by 
            visualizing unfolded proteins in the ER.
  JOURNAL   Cell Rep. 2024 Jun 25;43(6):114358. doi: 
            10.1016/j.celrep.2024.114358. Epub 2024 Jun 11.
  PUBMED    38865243
REFERENCE   2  (bases 1 to 10202)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep.";
            date: "2024-06-25"; volume: "43(6):114358. doi"; pages: " 
            10.1016/j.celrep.2024.114358. Epub 2024 Jun 11"
COMMENT     Sequence Label: pLVX-PERK(LD)-EGFP-HOTag3
FEATURES             Location/Qualifiers
     source          1..10202
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    680..805
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1302..1535
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1720..1764
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2027..2142
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     enhancer        2200..2503
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2504..2707
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2658..2678
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2704..2728
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      2820..2829
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4086..4796
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF
                     ICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVN
                     FKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEF
                     VTAAGITLGMDELYK"
     CDS             4086..4796
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF
                     ICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVN
                     FKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEF
                     VTAAGITLGMDELYK"
     primer_bind     complement(4125..4146)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4386..4405)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4733..4754
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     promoter        5036..5535
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(5071..5094)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     5468..5487
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             5556..6155
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5556..5575)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6052..6072
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    6169..6757
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6222..6242)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6640..6651)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6964..7597
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7726..7742)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7726..7742)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7739..7761)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7750..7766
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7774..7780,7781..7798,7799..7804))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7819..7840
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7957..7974)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8128..8716)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8208..8227)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8887..9678,9679..9747))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9510..9529
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9748..9852)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    9900..10034
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9937..9956)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9991..10010
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             join(10202,1..633)
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
ORIGIN
        1 ggaagggcta attcactccc aaagaagaca agatatcctt gatctgtgga tctaccacac
       61 acaaggctac ttccctgatt agcagaacta cacaccaggg ccaggggtca gatatccact
      121 gacctttgga tggtgctaca agctagtacc agttgagcca gataaggtag aagaggccaa
      181 taaaggagag aacaccagct tgttacaccc tgtgagcctg catgggatgg atgacccgga
      241 gagagaagtg ttagagtgga ggtttgacag ccgcctagca tttcatcacg tggcccgaga
      301 gctgcatccg gagtacttca agaactgctg atatcgagct tgctacaagg gactttccgc
      361 tggggacttt ccagggaggc gtggcctggg cgggactggg gagtggcgag ccctcagatc
      421 ctgcatataa gcagctgctt tttgcctgta ctgggtctct ctggttagac cagatctgag
      481 cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt
      541 gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca
      601 gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc ccgaacaggg acttgaaagc
      661 gaaagggaaa ccagaggagc tctctcgacg caggactcgg cttgctgaag cgcgcacggc
      721 aagaggcgag gggcggcgac tggtgagtac gccaaaaatt ttgactagcg gaggctagaa
      781 ggagagagat gggtgcgaga gcgtcagtat taagcggggg agaattagat cgcgatggga
      841 aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa ttaaaacata tagtatgggc
      901 aagcagggag ctagaacgat tcgcagttaa tcctggcctg ttagaaacat cagaaggctg
      961 tagacaaata ctgggacagc tacaaccatc ccttcagaca ggatcagaag aacttagatc
     1021 attatataat acagtagcaa ccctctattg tgtgcatcaa aggatagaga taaaagacac
     1081 caaggaagct ttagacaaga tagaggaaga gcaaaacaaa agtaagacca ccgcacagca
     1141 agcggccggc cgctgatctt cagacctgga ggaggagata tgagggacaa ttggagaagt
     1201 gaattatata aatataaagt agtaaaaatt gaaccattag gagtagcacc caccaaggca
     1261 aagagaagag tggtgcagag agaaaaaaga gcagtgggaa taggagcttt gttccttggg
     1321 ttcttgggag cagcaggaag cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc
     1381 agacaattat tgtctggtat agtgcagcag cagaacaatt tgctgagggc tattgaggcg
     1441 caacagcatc tgttgcaact cacagtctgg ggcatcaagc agctccaggc aagaatcctg
     1501 gctgtggaaa gatacctaaa ggatcaacag ctcctgggga tttggggttg ctctggaaaa
     1561 ctcatttgca ccactgctgt gccttggaat gctagttgga gtaataaatc tctggaacag
     1621 atttggaatc acacgacctg gatggagtgg gacagagaaa ttaacaatta cacaagctta
     1681 atacactcct taattgaaga atcgcaaaac cagcaagaaa agaatgaaca agaattattg
     1741 gaattagata aatgggcaag tttgtggaat tggtttaaca taacaaattg gctgtggtat
     1801 ataaaattat tcataatgat agtaggaggc ttggtaggtt taagaatagt ttttgctgta
     1861 ctttctatag tgaatagagt taggcaggga tattcaccat tatcgtttca gacccacctc
     1921 ccaaccccga ggggacccga caggcccgaa ggaatagaag aagaaggtgg agagagagac
     1981 agagacagat ccattcgatt agtgaacgga tctcgacggt atcgccttta aaagaaaagg
     2041 ggggattggg gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca
     2101 aactaaagaa ttacaaaaac aaattacaaa aattcaaaat tttcgggttt attacaggga
     2161 cagcagagat ccagtttatc gataagcttg ggagttccgc gttacataac ttacggtaaa
     2221 tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt
     2281 tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta
     2341 aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt
     2401 caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc
     2461 tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc ggttttggca
     2521 gtacatcaat gggcgtggat agcggtttga ctcacgggga tttccaagtc tccaccccat
     2581 tgacgtcaat gggagtttgt tttggcacca aaatcaacgg gactttccaa aatgtcgtaa
     2641 caactccgcc ccattgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag
     2701 cagagctcgt ttagtgaacc gtcagatcgc ctggagacgc catccacgct gttttgacct
     2761 ccatagaaga caccgactct actagaggat cgctagcgct accggactca gatctcgagg
     2821 ccgccatgga gcgcgccacc cggcccgggc cgcgcgcgct gctgctgctt ctgttcctgc
     2881 tgctgggctg cgcggcgggg atctcggcgg tcgcgcccgc ccgcagtttg cttgctcccg
     2941 cgtcggagac agtgtttggc ttaggggcag cggccgcccc gacttcggcc gcgcgggtgc
     3001 ctgcggtggc aacggccgaa gtgaccgtgg aggacgccga ggcattgccg gctgccgctg
     3061 gcgaaccgga gtcacgcgcg acggagcccg atgacgacgt ggaactgcgg cctcgcggca
     3121 ggtccttggt aatcatcagc actttagatg gacgaatcgc tgcactggat gccgagaatg
     3181 atgggaaaaa gcagtgggat ttggacgtgg ggtctggttc cttggtttca tctagcctca
     3241 gcaagccaga ggtgtttggg aacaagatga tcatcccctc cctggatgga gacctcttcc
     3301 agtgggaccg ggaccgagag agcatggagg ccgtcccctt cacggtggag tccctgctcg
     3361 aatcttccta caagtttgga gatgatgttg ttctggttgg agggaaatct ctgactacat
     3421 acggactcag tgcttacagt ggaaagctga ggtatatctg ttctgccttg ggatgtcgcc
     3481 gatgggatag tgatgaaatg gaagaagagg aagacatctt gcttctgcag cgtacgcaga
     3541 agactgtgcg agctgtcggg cctcgaagcg gcagtgagaa gtggaatttc agtgttggcc
     3601 actttgaact tcggtatatt ccagacatgg aaactagagc cggattcatt gaaagcacct
     3661 ttaaaccggg tggaaacaaa gaagactcta aaattatttc agatgtggaa gaacaagaag
     3721 ccaccatgct ggacacagtg ataaaagttt ccgttgctga ttggaaggtc atggcgttta
     3781 gtaggaaggg aggccgcctg gaatgggagt accagttttg tactcccatc gcgtccgcct
     3841 ggctggtgag ggatggcaag gtcatcccca tcagcctgtt tgatgataca agttacacag
     3901 ccagcgaaga agccttggga gacgaagaag acattgtaga ggctgctcgg ggagccacag
     3961 agaacagcgt gtacttaggg atgtacagag gccagctgta cctgcagtcg tccgtcaggg
     4021 tctcagaaaa gttccctaca agcccaaagg ccttggagtc tgtaaatggc gaaggtagtg
     4081 gaagcagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc gagctggacg
     4141 gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg
     4201 gcaagctgac cctgaagttc atctgcacca ccggcaagct gcccgtgccc tggcccaccc
     4261 tcgtgaccac cctgacctac ggcgtgcagt gcttcagccg ctaccccgac cacatgaagc
     4321 agcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc accatcttct
     4381 tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg
     4441 tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc ctggggcaca
     4501 agctggagta caactacaac agccacaacg tctatatcat ggccgacaag cagaagaacg
     4561 gcatcaaggt gaacttcaag atccgccaca acatcgagga cggcagcgtg cagctcgccg
     4621 accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc gacaaccact
     4681 acctgagcac ccagtccgcc ctgagcaaag accccaacga gaagcgcgat cacatggtcc
     4741 tgctggagtt cgtgaccgcc gccgggatca ctctcggcat ggacgagctg tacaagggat
     4801 ccggcagtgc tggtggtagt gctggtggta gtgctggtgg tagtgctggt ggcagtgctg
     4861 gtggtagtgc tggtggtagt gctggtggct ctagaggaga aatcgcaaag agtctgaaag
     4921 aaattgcaaa atccttgaag gaaatagcgt ggtcactgaa agaaattgcc aagtccctca
     4981 aagggaaaga tgaactgtaa gggcccggga tcccgcgact ctagataatt ctaccgggta
     5041 ggggaggcgc ttttcccaag gcagtctgga gcatgcgctt tagcagcccc gctgggcact
     5101 tggcgctaca caagtggcct ctggcctcgc acacattcca catccaccgg taggcgccaa
     5161 ccggctccgt tctttggtgg ccccttcgcg ccaccttcta ctcctcccct agtcaggaag
     5221 ttcccccccg ccccgcagct cgcgtcgtgc aggacgtgac aaatggaagt agcacgtctc
     5281 actagtctcg tgcagatgga cagcaccgct gagcaatgga agcgggtagg cctttggggc
     5341 agcggccaat agcagctttg ctccttcgct ttctgggctc agaggctggg aaggggtggg
     5401 tccgggggcg ggctcagggg cgggctcagg ggcggggcgg gcgcccgaag gtcctccgga
     5461 ggcccggcat tctgcacgct tcaaaagcgc acgtctgccg cgctgttctc ctcttcctca
     5521 tctccgggcc tttcgacctg cagcccaagc ttaccatgac cgagtacaag cccacggtgc
     5581 gcctcgccac ccgcgacgac gtccccaggg ccgtacgcac cctcgccgcc gcgttcgccg
     5641 actaccccgc cacgcgccac accgtcgatc cggaccgcca catcgagcgg gtcaccgagc
     5701 tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg gtcgcggacg
     5761 acggcgccgc ggtggcggtc tggaccacgc cggagagcgt cgaagcgggg gcggtgttcg
     5821 ccgagatcgg cccgcgcatg gccgagttga gcggttcccg gctggccgcg cagcaacaga
     5881 tggaaggcct cctggcgccg caccggccca aggagcccgc gtggttcctg gccaccgtcg
     5941 gcgtctcgcc cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg
     6001 aggcggccga gcgcgccggg gtgcccgcct tcctggagac ctccgcgccc cgcaacctcc
     6061 ccttctacga gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc gaaggaccgc
     6121 gcacctggtg catgacccgc aagcccggtg cctgaccgcg tctggaacaa tcaacctctg
     6181 gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta
     6241 tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt
     6301 ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc
     6361 aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt
     6421 gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg
     6481 gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac
     6541 aattccgtgg tgttgtcggg gaagctgacg tcctttccat ggctgctcgc ctgtgttgcc
     6601 acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac
     6661 cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct
     6721 cagacgagtc ggatctccct ttgggccgcc tccccgcctg gaattaattc tgcagtcgag
     6781 acctagaaaa acatggagca atcacaagta gcaatacagc agctaccaat gctgattgtg
     6841 cctggctaga agcacaagag gaggaggagg tgggttttcc agtcacacct caggtacctt
     6901 taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaagaggg
     6961 gactggaagg gctaattcac tcccaacgaa gacaagatat ccttgatctg tggatctacc
     7021 acacacaagg ctacttccct gattagcaga actacacacc agggccaggg gtcagatatc
     7081 cactgacctt tggatggtgc tacaagctag taccagttga gccagataag gtagaagagg
     7141 ccaataaagg agagaacacc agcttgttac accctgtgag cctgcatggg atggatgacc
     7201 cggagagaga agtgttagag tggaggtttg acagccgcct agcatttcat cacgtggccc
     7261 gagagctgca tccggagtac ttcaagaact gctgatatcg agcttgctac aagggacttt
     7321 ccgctgggga ctttccaggg aggcgtggcc tgggcgggac tggggagtgg cgagccctca
     7381 gatcctgcat ataagcagct gctttttgcc tgtactgggt ctctctggtt agaccagatc
     7441 tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg
     7501 ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc
     7561 ctcagaccct tttagtcagt gtggaaaatc tctagcagta gtagttcatg tcatcttatt
     7621 attcagtatt tataacttgc aaagaaatga atatcagaga gtgagaggcc ttgacattgc
     7681 tagcgtttta ccgtcgacct ctagctagag cttggcgtaa tcatggtcat agctgtttcc
     7741 tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg
     7801 taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc
     7861 cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg
     7921 gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc
     7981 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
     8041 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
     8101 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
     8161 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
     8221 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
     8281 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
     8341 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
     8401 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
     8461 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
     8521 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg
     8581 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
     8641 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
     8701 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
     8761 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
     8821 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
     8881 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
     8941 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
     9001 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc
     9061 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
     9121 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
     9181 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc
     9241 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
     9301 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
     9361 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
     9421 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
     9481 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa
     9541 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
     9601 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt
     9661 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
     9721 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta
     9781 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
     9841 aggggttccg cgcacatttc cccgaaaagt gccacctgac gtcgacggat cgggagatca
     9901 acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa
     9961 ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt
    10021 atcatgtctg gatcaactgg ataactcaag ctaaccaaaa tcatcccaaa cttcccaccc
    10081 cataccctat taccactgcc aattacctgt ggtttcattt actctaaacc tgtgattcct
    10141 ctgaattatt ttcattttaa agaaattgta tttgttaaat atgtactaca aacttagtag
    10201 tt
//
