LOCUS       sequence-449363-        2818 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Control cassette FIRE2, FIRE fluorescent ratiometric sensor.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2818)
  AUTHORS   Sangokoya C
  TITLE     The FIRE biosensor illuminates iron regulatory protein activity and 
            cellular iron homeostasis.
  JOURNAL   Cell Rep Methods. 2025 Jan 11:100960. doi: 
            10.1016/j.crmeth.2024.100960.
  PUBMED    39824193
REFERENCE   2  (bases 1 to 2818)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep 
            Methods. 2025 Jan 11:100960. doi: 10.1016/j.crmeth.2024.100960."
COMMENT     Sequence Label: FIREC-PBCS-MR3
FEATURES             Location/Qualifiers
     source          1..2818
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     repeat_region   17..51
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     polyA_signal    351..406
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     regulatory      1151..1160
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(1192..2370)
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          1201..2139
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     complement(1224..1244)
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     repeat_region   2740..2802
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
ORIGIN
        1 cgcaaatctt ttttaaccct agaaagatag tctgcgtaaa attgacgcat gcattcttga
       61 aatattgctc tctctttcta aatagcgcga atccgtcgct gtgcatttag gacatctcag
      121 tcgccgcttg gagctcccgt gaggcgtgct tgtcaatgcg gtaagtgtca ctgattttga
      181 actataacga ccgcgtgagt caaaatgacg catgattatc ttttacgtga cttttaagat
      241 ttaactcata cgataattat attgttattt catgttctac ttacgtgata acttattata
      301 tatatatttt cttgttatag atgcttacgt aacgcgtgtt aaccctcgag tgagagacac
      361 aaaaaattcc aacacactat tgcaatgaaa ataaatttcc tttattcaat tggtttcccg
      421 gggttaacgt ttaaacaccg gttcttatac agctcatcca tccctccccc cagattactg
      481 tattttgcga ctgctacctc tctctgtact acataggtct cattgtcact ctcctcaatt
      541 cgctcgagcc tatgatccac agcgtggacc ccaggcatct tgatattccc aactgtcttc
      601 ttgcttctgt aggtagtaac aaaattacaa tgcaggtgtc cacctccatc aaccttcagt
      661 gcaatgtcgg tgtagccccg caaaccccca tcagctggat acatcatttc agtatttggt
      721 tcccaaccct ttgttttctt ctgcatcaca ggtccgttgc tggggaaatt aactcctctt
      781 accttcacat tatagacaag ttcaccatct tcaaggctgg tgtcttgagt cacagtgacg
      841 actccgccat cctcgtagcg tgtgacgcgt tcccaagtaa aaccctctgg aaaggattgc
      901 ttaaaaaagt caggaatatc tgctggatac ttaataaaag ttcttgaacc atacatgaat
      961 gaggtagcca atatatcgaa ggcaaaaggc aaaggccccc cctcaatcac ttttattcgc
     1021 attgtttgtg ttccctcata aggtcgacct tctccttcac cggtacactt aaattgatgt
     1081 ccgttgacac tcccctccat gactaccttc atcctcatat tttcctttat caattcttcc
     1141 cctttagaca ccatggcggc aaacaaacgc ggccgcgacg tccccctata gtcacgacac
     1201 ctgaaatgga agaaaaaaac tttgaaccac tgtctgaggc ttgagaatga accaagatcc
     1261 aaactcaaaa agggcaaatt ccaaggagaa ttacatcaag tgccaagctg gcctaacttc
     1321 agtctccacc cactcagtgt ggggaaactc catcgcataa aacccctccc cccaacctaa
     1381 agacgacgta ctccaaaagc tcgagaacta atcgaggtgc ctggacggcg cccggtactc
     1441 cgtggagtca catgaagcga cggctgagga cggaaaggcc cttttccttt gtgtgggtga
     1501 ctcacccgcc cgctctcccg agcgccgcgt cctccatttt gagctccctg cagcagggcc
     1561 gggaagcggc catctttccg ctcacgcaac tggtgccgac cgggccagcc ttgccgccca
     1621 gggcggggcg atacacggcg gcgcgaggcc aggcaccaga gcaggccggc cagcttgaga
     1681 ctacccccgt ccgattctcg gtggccgcgc tcgcaggccc cgcctcgccg aacatgtgcg
     1741 ctgggacgca cgggccccgt cgccgcccgc ggccccaaaa accgaaatac cagtgtgcag
     1801 atcttggccc gcatttacaa gactatcttg ccagaaaaaa agcgtcgcag caggtcatca
     1861 aaaattttaa atggctagag acttatcgaa agcagcgaga caggcgcgaa ggtgccacca
     1921 gattcgcacg cggcggcccc agcgcccagg ccaggcctca actcaagcac gaggcgaagg
     1981 ggctccttaa gcgcaaggcc tcgaactctc ccacccactt ccaacccgaa gctcgggatc
     2041 aagaatcacg tactgcagcc aggtggaagt aattcaaggc acgcaagggc cataacccgt
     2101 aaagaggcca ggcccgcggg aaccacacac ggcacttacc tgtgttctgg cggcaaaccc
     2161 gttgcgaaaa agaacgttca cggcgactac tgcacttata tacggttctc ccccaccctc
     2221 gggaaaaagg cggagccagt acacgacatc actttcccag tttaccccgc gccaccttct
     2281 ctaggcaccg gttcaattgc cgacccctcc ccccaacttc tcggggactg tgggcgatgt
     2341 gcgctctgcc cactgacggg caccggagcc tcacgtgtac aggggctagc gaattcttaa
     2401 ttaacgagag cataatattg atatgtgcca aagttgtttc tgactgacta ataagtataa
     2461 tttgtttcta ttatgtatag gttaagctaa ttacttattt tataatacaa catgactgtt
     2521 tttaaagtac aaaataagtt tatttttgta aaagagagaa tgtttaaaag ttttgttact
     2581 ttatagaaga aattttgagt ttttgttttt ttttaataaa taaataaaca taaataaatt
     2641 gtttgttgaa tttattatta gtatgtaagt gtaaatataa taaaacttaa tatctattca
     2701 aattaataaa taaacctcga tatacagacc gataaaacac atgcgtcaat tttacgcatg
     2761 attatcttta acgtacgtca caatatgatt atctttctag ggttaaataa tagtttct
//
