LOCUS       sequence-449366-        3054 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For gateway cloning of N-terminal tagged MACROD1 constructs..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3054)
  AUTHORS   Zaja R, Aydin G, Lippok BE, Feederle R, Luscher B, Feijs KLH
  TITLE     Comparative analysis of MACROD1, MACROD2 and TARG1 expression, 
            localisation and interactome.
  JOURNAL   Sci Rep. 2020 May 19;10(1):8286. doi: 10.1038/s41598-020-64623-y.
  PUBMED    32427867
REFERENCE   2  (bases 1 to 3054)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2020-05-19"; volume: "10(1):8286. doi"; pages: " 
            10.1038/s41598-020-64623-y"
COMMENT     Sequence Label: pDONR/zeo-MACROD1
FEATURES             Location/Qualifiers
     source          1..3054
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     94..111
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(270..297)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(389..475)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     524..546
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     538..555
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     539..555
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    571..670
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     regulatory      917..926
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(1652..1751)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1768..1787)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1769..1787)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1792..1808)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1792..1808)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             complement(1876..2250)
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     promoter        complement(2269..2316)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     rep_origin      2406..2994
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2895..2914
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
       61 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
      121 gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg
      181 cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tacgcgtacc
      241 gctagccagg aagagtttgt agaaacgcaa aaaggccatc cgtcaggatg gccttctgct
      301 tagtttgatg cctggcagtt tatggcgggc gtcctgcccg ccaccctccg ggccgttgct
      361 tcacaacgtt caaatccgct cccggcggat ttgtcctact caggagagcg ttcaccgaca
      421 aacaacagat aaaacgaaag gcccagtctt ccgactgagc ctttcgtttt atttgatgcc
      481 tggcagttcc ctactctcgc gttaacgcta gcatggatgt tttcccagtc acgacgttgt
      541 aaaacgacgg ccagtcttaa gctcgggccc caaataatga ttttattttg actgatagtg
      601 acctgttcgt tgcaacaaat tgatgagcaa tgctttttta taatgccaac tttgtacaaa
      661 aaagcaggct tcatgtctct acagagccga ctgtccggcc gcctggcaca gctgcgcgcg
      721 gcggggcagc tgctcgtccc cccgcgcccc cggcccggac acttggcggg tgccacgagg
      781 acccgcagca gcacgtgcgg tcccccggcg ttcctgggcg tgttcggccg ccgtgcgcgg
      841 acctcggcgg gagttggggc gtggggggcg gcggcggtgg ggcggacagc cggggtgcgc
      901 acttgggccc ccctggccat ggcggcgaag gtggacctga gcacctccac cgactggaag
      961 gaggcgaaat cctttctgaa gggcctgagt gacaagcagc gggaggaaca ttacttctgc
     1021 aaggactttg tcaggctgaa gaagatcccg acatggaagg agatggcgaa aggggtggct
     1081 gtgaaggtgg aggagcccag gtataaaaag gacaagcagc tcaatgagaa aatctccctg
     1141 ctccgcagcg acatcaccaa gctggaggtg gacgccatcg tcaacgccgc caacagctcc
     1201 ctgctcggag gcggtggcgt ggacggctgc attcatcggg ccgccggccc cctgcttacc
     1261 gacgagtgcc ggaccctgca gagctgtaag actggcaagg ccaagatcac cggcggctat
     1321 cggctcccgg ccaagtacgt catccacaca gtggggccca tcgcctacgg ggagcccagc
     1381 gccagccagg ctgccgagct ccgcagctgc tacctgagca gtctggacct gctgctggag
     1441 caccggctcc gctcggtggc gttcccctgc atctccaccg gcgtgtttgg ctacccctgt
     1501 gaggcggccg ccgagatcgt gctggccacg ctgcgagagt ggctggagca gcacaaggac
     1561 aaggtggacc ggctgatcat ctgcgtgttc ctcgagaagg acgaggacat ctaccggagc
     1621 cggctccccc actacttccc cgtggcctga gacccagctt tcttgtacaa agttggcatt
     1681 ataagaaagc attgcttatc aatttgttgc aacgaacagg tcactatcag tcaaaataaa
     1741 atcattattt gccatccagc tgatatcccc tatagtgagt cgtattacat ggtcatagct
     1801 gtttcctggc agctctggcc cgtgtctcaa aatctctgat gttacattgc acaagataaa
     1861 aatatatcat catgatcagt cctgctcctc ggccacgaag tgcacgcagt tgccggccgg
     1921 gtcgcgcagg gcgaactccc gcccccacgg ctgctcgccg atctcggtca tggccggccc
     1981 ggaggcgtcc cggaagttcg tggacacgac ctccgaccac tcggcgtaca gctcgtccag
     2041 gccgcgcacc cacacccagg ccagggtgtt gtccggcacc acctggtcct ggaccgcgct
     2101 gatgaacagg gtcacgtcgt cccggaccac accggcgaag tcgtcctcca cgaagtcccg
     2161 ggagaacccg agccggtcgg tccagaactc gaccgctccg gcgacgtcgc gcgcggtgag
     2221 caccggaacg gcactggtca acttggccat ggtttagttc ctcaccttgt cgtattatac
     2281 tatgccgata tactatgccg atgattaatt gtcaacacgt gctgatcatg accaaaatcc
     2341 cttaacgtga gttacgcgtc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     2401 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     2461 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     2521 ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac
     2581 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
     2641 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
     2701 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
     2761 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
     2821 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
     2881 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
     2941 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
     3001 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catg
//
