LOCUS       sequence-449367-       15310 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  To clone any gene of interest using gateway recombination cloning 
            and the vector expresses seed-specific RUBY.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15310)
  TITLE     Siddiqi August 2024 Plasmids
REFERENCE   2  (bases 1 to 15310)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pGWG_RUBYseed
FEATURES             Location/Qualifiers
     source          1..15310
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    115..139
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     protein_bind    210..334
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(359..364,365..382,383..389)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             443..1102
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(509..528)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             1444..1749
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     1628..1647
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(1790..1914)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             2520..6470
                     /label=RUBY
     CDS             4020..4076
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             4911..4967
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     primer_bind     complement(7067..7084)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7067..7083)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7076..7098)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     promoter        7312..7988
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     primer_bind     7925..7945
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             8051..8584
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MVRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     polyA_signal    8641..8815
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    8893..8917
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             9438..10229
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      10475..11063
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10964..10983
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11217..11234
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    11249..11389
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(11315..11334)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     11475..11497
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      11733..11927
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(11993..13066)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(13495..14124)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 ttgatcccga ggggaaccct gtggttggca tgcacataca aatggacgaa cggataaacc
       61 ttttcacgcc cttttaaata tccgattatt ctaataaacg ctcttttctc ttaggtttac
      121 ccgccaatat atcctgtcaa acactgatag tttaaactga aggcgggaaa cgacaatctg
      181 atccaagctc aagctgctct agtgatatca caagtttgta caaaaaagct gaacgagaaa
      241 cgtaaaatga tataaatatc aatatattaa attagatttt gcataaaaaa cagactacat
      301 aatactgtaa aacacaacat atccagtcac tatggcggcc gcattaggca ccccaggctt
      361 tacactttat gcttccggct cgtataatgt gtggattttg agttaggatc cgtcgagatt
      421 ttcaggagct aaggaagcta aaatggagaa aaaaatcact ggatatacca ccgttgatat
      481 atcccaatgg catcgtaaag aacattttga ggcatttcag tcagttgctc aatgtaccta
      541 taaccagacc gttcagctgg atattacggc ctttttaaag accgtaaaga aaaataagca
      601 caagttttat ccggccttta ttcacattct tgcccgcctg atgaatgctc atccggaatt
      661 ccgtatggca atgaaagacg gtgagctggt gatatgggat agtgttcacc cttgttacac
      721 cgttttccat gagcaaactg aaacgttttc atcgctctgg agtgaatacc acgacgattt
      781 ccggcagttt ctacacatat attcgcaaga tgtggcgtgt tacggtgaaa acctggccta
      841 tttccctaaa gggtttattg agaatatgtt tttcgtctca gccaatccct gggtgagttt
      901 caccagtttt gatttaaacg tggccaatat ggacaacttc ttcgcccccg ttttcaccat
      961 gggcaaatat tatacgcaag gcgacaaggt gctgatgccg ctggcgattc aggttcatca
     1021 tgccgtttgt gatggcttcc atgtcggcag aatgcttaat gaattacaac agtactgcga
     1081 tgagtggcag ggcggggcgt aaacgcgtgg atccggctta ctaaaagcca gataacagta
     1141 tgcgtatttg cgcgctgatt tttgcggtat aagaatatat actgatatgt atacccgaag
     1201 tatgtcaaaa agaggtatgc tatgaagcag cgtattacag tgacagttga cagcgacagc
     1261 tatcagttgc tcaaggcata tatgatgtca atatctccgg tctggtaagc acaaccatgc
     1321 agaatgaagc ccgtcgtctg cgtgccgaac gctggaaagc ggaaaatcag gaagggatgg
     1381 ctgaggtcgc ccggtttatt gaaatgaacg gctcttttgc tgacgagaac aggggctggt
     1441 gaaatgcagt ttaaggttta cacctataaa agagagagcc gttatcgtct gtttgtggat
     1501 gtacagagtg atattattga cacgcccggg cgacggatgg tgatccccct ggccagtgca
     1561 cgtctgctgt cagataaagt ctcccgtgaa ctttacccgg tggtgcatat cggggatgaa
     1621 agctggcgca tgatgaccac cgatatggcc agtgtgccgg tctccgttat cggggaagaa
     1681 gtggctgatc tcagccaccg cgaaaatgac atcaaaaacg ccattaacct gatgttctgg
     1741 ggaatataaa tgtcaggctc ccttatacac agccagtctg caggtcgacc atagtgactg
     1801 gatatgttgt gttttacagt attatgtagt ctgtttttta tgcaaaatct aatttaatat
     1861 attgatattt atatcatttt acgtttctcg ttcagctttc ttgtacaaag tggtgatatc
     1921 ccgcggccat gctagagtcc gcaaaaatca ccagtctctc tctacaaatc tatctctctc
     1981 tatttttctc cagaataatg tgtgagtagt tcccagataa gggaattagg gttcttatag
     2041 ggtttcgctc atgtgttgag catataagaa acccttagta tgtatttgta tttgtaaaat
     2101 acttctatca ataaaatttc taattcctaa aaccaaaatc cagtgacctg caggcatgcg
     2161 acgtcgggcc ctaaataaaa gtttaacctt ctttttaaaa aattgttcat agtgtcatgt
     2221 cagaacaaga gctacaaatc acacatagca tgcataagcg gagctatgat gagtggtatt
     2281 gttttgttcg tcacttgtca ctcttttcca acacataatc ccgacaacaa cgtaagagca
     2341 tctctctctc tccacacaca ctcatgcatg catgcattct tacacgtgat tgccatgcaa
     2401 atctcctttc tcacctataa atacaaacca acccttcact acactcttca ctcaaaccaa
     2461 aacaagaaaa catacacaaa tagcaaaacg gtaccaacaa aactagtggg ccctctagta
     2521 tggatcatgc gaccctcgcc atgatcctcg cgatctggtt catcagcttc cacttcatca
     2581 agctgctgtt ctcccagcag accaccaagc tgcttccgcc aggaccaaag ccgcttccga
     2641 tcatcggcaa catccttgag gtgggcaaga agccgcatcg gtccttcgcc aacctcgcca
     2701 agattcacgg cccactcatt tccctcagac tcggctctgt gaccaccatc gttgtgtcct
     2761 ctgccgacgt ggccaaagag atgttcctca agaaggatca cccgctctcc aaccgcacga
     2821 tcccgaatag tgttacagcc ggcgaccacc acaagctcac catgtcttgg ctcccggtgt
     2881 ctccgaagtg gcgcaacttc cgcaagatta ccgccgtgca tctgctctcc ccacagagac
     2941 tcgatgcctg ccagacattc aggcacgcca aggtgcagca gctctacgag tacgttcaag
     3001 agtgcgccca gaaaggccag gccgtggata ttggcaaggc cgcctttacg accagcctca
     3061 acctcctcag caagctgttc ttcagcgtcg agctggcgca ccacaagtcc cataccagcc
     3121 aagagttcaa agagctgatc tggaacatca tggaagatat aggcaagccg aactacgccg
     3181 actacttccc gattctcggc tgcgttgacc catctggcat tagaagaagg ctcgcctgct
     3241 ccttcgacaa gctgatcgcc gtgttccagg gcatcatctg cgagagactc gccccagatt
     3301 cctccaccac aactaccacc accaccgacg acgtgctcga tgtgctcctc cagctgttca
     3361 agcagaacga gctgacgatg ggcgagatca accacctcct cgtggacatc ttcgacgccg
     3421 gcaccgatac cacatcctcc acattcgagt gggtgatgac cgagctgatc cgcaatccag
     3481 agatgatgga aaaggcccaa gaggaaatca agcaggtcct cggcaaggac aagcagatcc
     3541 aagagtccga catcatcaac ctgccgtacc tccaggcgat catcaaagag acactccgcc
     3601 tccatccgcc gaccgtgttc ttgctcccaa gaaaggccga caccgatgtc gagctgtacg
     3661 gctacatcgt gccgaaggat gcccagatcc tcgtgaacct ctgggccatt ggcagggacc
     3721 caaacgcctg gcagaacgcc gatattttca gcccagagcg cttcatcggc tgcgagatcg
     3781 atgttaaggg ccgcgatttc ggcctccttc catttggcgc tggccgcaga atttgcccag
     3841 gcatgaatct cgccatcagg atgctcaccc tcatgctcgc cacactcctc cagttcttca
     3901 actggaagct cgaaggcgac atctccccga aggacctcga catggacgag aagttcggca
     3961 ttgcgctcca aaagaccaag ccgctcaagc tcatcccgat tccgcgctac ggtagcggag
     4021 ctaccaattt tagcctcctt aagcaggcag gtgatgtaga agagaacccc gggcctatga
     4081 agatgatgaa cggcgaggac gccaacgacc agatgatcaa agagtccttc ttcatcaccc
     4141 acggcaaccc gatcctcacc gtcgaggata cacatccgct caggccgttc ttcgagacat
     4201 ggcgcgagaa gattttctcc aagaagccga aggccatcct catcatctcc ggccactggg
     4261 agacagtgaa gccaaccgtg aacgccgtgc acatcaacga caccatccac gacttcgacg
     4321 actacccagc cgccatgtac cagttcaagt acccagctcc aggcgagcca gagcttgcga
     4381 gaaaggtgga agagatcctc aagaagtccg ggttcgagac agccgagaca gaccaaaaga
     4441 ggggccttga tcacggcgcc tgggttccac tcatgctcat gtatccagag gcggacatcc
     4501 cggtgtgcca gctctcagtt cagccacatc tcgacggcac ctaccactac aatctcggca
     4561 gagccctcgc gccgctcaag aatgatggcg tgctcattat tggctccggc agcgccacac
     4621 atccactcga tgagacaccg cactacttcg atggtgttgc cccttgggcc gctgccttcg
     4681 attcttggct taggaaggcc ctcatcaacg gccgcttcga ggaagtgaac atctacgaga
     4741 gcaaggcccc gaactggaag ctcgcccatc catttccaga gcacttctac ccgctccacg
     4801 ttgtgctcgg cgctgctggt gaaaagtgga aggccgagct gatccactcc tcctgggatc
     4861 atggcacact ttgccacggc tcctacaagt tcacctccgc cggatccgga gcaaccaact
     4921 ttagcctgct caagcaagca ggagatgttg aggaaaatcc tggccccatg accgccatca
     4981 agatgaacac caacggcgag ggcgagacac agcacatcct catgatcccg ttcatggcgc
     5041 agggccacct caggccattt ctcgaactcg ccatgttcct ctacaagcgc tcccacgtga
     5101 tcatcaccct gctcacaact ccgctcaatg ccggcttcct caggcacctc cttcaccacc
     5161 attcctactc ctccagcggc atcaggatcg tcgagctgcc attcaactcc accaaccacg
     5221 gactcccacc gggcatcgag aacaccgata agctcacact cccgctcgtg gtgtccctct
     5281 tccattccac catcagcctc gatccgcacc tccgcgatta catctccagg catttcagcc
     5341 cagccaggcc accactctgc gtgatccatg atgtgttcct cggctgggtt gaccaggtgg
     5401 ccaaggatgt gggctctaca ggcgtggtgt tcacaacagg cggcgcttat ggcacatccg
     5461 cctacgtgtc catctggaac gatctcccgc accagaacta ctccgacgac caagagttcc
     5521 cgctgccagg cttcccagag aaccataagt tccgcaggtc ccagctccat cggttcctca
     5581 gatatgccga cggctccgac gattggtcca agtatttcca gccgcagctc cgccagtcca
     5641 tgaagtcttt tggctggctc tgcaactccg tggaagagat cgagacactc ggcttctcca
     5701 tcctccgcaa ctacaccaag ctgccgatct ggggcatcgg cccacttatt gcttccccag
     5761 tgcagcactc ctcctccgac aacaattcaa caggcgccga gttcgtgcag tggctcagcc
     5821 tcaaagagcc ggactccgtc ctctacatct ccttcggctc ccagaacacg atcagcccga
     5881 cgcagatgat ggaactcgct gctggccttg agtcctccga gaagccattc ctctgggtga
     5941 tcagagcccc gttcggcttc gacatcaacg aagagatgcg cccagagtgg ctgccagagg
     6001 gctttgagga acgcatgaag gtgaagaaac agggcaagct cgtgtacaag ctcggcccgc
     6061 agcttgagat cctcaaccat gaatccatcg gcggctttct cacccactgc ggatggaaca
     6121 gcatccttga gtctcttcgc gagggcgttc cgatgcttgg atggccactt gctgccgagc
     6181 aggcctacaa cctcaagtac ctcgaagatg agatgggcgt cgcggttgag cttgctagag
     6241 gcctcgaagg cgagatctcc aaagagaagg tcaagcgcat cgtcgagatg atccttgagc
     6301 gcaacgaggg ctccaaaggc tgggagatga agaatcgcgc cgtggaaatg ggcaaaaagc
     6361 tcaaggacgc cgtgaacgag gaaaaagagc tgaagggctc ctccgtgaag gcgatcgacg
     6421 atttcctcga cgccgtcatg caggccaaac ttgagccaag cctccagtga tagtgaatat
     6481 gaagatgaag atgaaatatt tggtgtgtca aataaaaagg ttgtgtgctt aagtttgtgt
     6541 ttttttcttg gcttgttgtg ttatgaattt gtggcttttt ctaatattaa atgaatgtaa
     6601 catctcatta taatgaataa acaaatgttt ctataatcca ttgtgaatgt tttgttggat
     6661 ctcttctcca gcatataact actgtatgtg ctatggtatg gactatggaa tatgattaaa
     6721 gataagacct aactgagtaa gctagcttgt tcgagtatta tggcattggg aaaactgttt
     6781 ttcttgtacc atttgttgtg cttgtaattt actgtgtttt ttattcggtt ttcgctatcg
     6841 aactgtgaaa tggaaatgga tggagaagag ttaatgaatg atatggtcct tttgttcatt
     6901 ctcaaattaa tattatttgt tttttctctt atttgttgtg tgttgaattt gaaattataa
     6961 gagatatgca aacattttgt tttgagtaaa aatgtgtcaa atcgtggcct ctaatgaccg
     7021 aagttaatat gaggagtaaa acactagatc cccaaacaag cttggcactg gccgtcgttt
     7081 tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc
     7141 cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt
     7201 tgcgcagcct gaatggcgaa tgctagagca gcttgccaac atggtggagc acgacactct
     7261 cgtctactcc aagaatatca aagatacagt ctcagaagac caaagggcta ttgagacttt
     7321 tcaacaaagg gtaatatcgg gaaacctcct cggattccat tgcccagcta tctgtcactt
     7381 catcaaaagg acagtagaaa aggaaggtgg cacctacaaa tgccatcatt gcgataaagg
     7441 aaaggctatc gttcaagatg cctctgccga cagtggtccc aaagatggac ccccacccac
     7501 gaggagcatc gtggaaaaag aagacgttcc aaccacgtct tcaaagcaag tggattgatg
     7561 tgaacatggt ggagcacgac actctcgtct actccaagaa tatcaaagat acagtctcag
     7621 aagaccaaag ggctattgag acttttcaac aaagggtaat atcgggaaac ctcctcggat
     7681 tccattgccc agctatctgt cacttcatca aaaggacagt agaaaaggaa ggtggcacct
     7741 acaaatgcca tcattgcgat aaaggaaagg ctatcgttca agatgcctct gccgacagtg
     7801 gtcccaaaga tggaccccca cccacgagga gcatcgtgga aaaagaagac gttccaacca
     7861 cgtcttcaaa gcaagtggat tgatgtgata tctccactga cgtaagggat gacgcacaat
     7921 cccactatcc ttcgcaagac ccttcctcta tataaggaag ttcatttcat ttggagagga
     7981 cacgctgaaa tcaccagtct ctctctacaa atctatctct ctcgagcttt cgcagatctg
     8041 tcgatcgacc atggtacgca gcagcaacga tgttacgcag cagggcagtc gccctaaaac
     8101 aaagttaggt ggctcaagta tgggcatcat tcgcacatgt aggctcggcc ctgaccaagt
     8161 caaatccatg cgggctgctc ttgatctttt cggtcgtgag ttcggagacg tagccaccta
     8221 ctcccaacat cagccggact ccgattacct cgggaacttg ctccgtagta agacattcat
     8281 cgcgcttgct gccttcgacc aagaagcggt tgttggcgct ctcgcggctt acgttctgcc
     8341 caagtttgag cagccgcgta gtgagatcta tatctatgat ctcgcagtct ccggcgagca
     8401 ccggaggcag ggcattgcca ccgcgctcat caatctcctc aagcatgagg ccaacgcgct
     8461 tggtgcttat gtgatctacg tgcaagcaga ttacggtgac gatcccgcag tggctctcta
     8521 tacaaagttg ggcatacggg aagaagtgat gcactttgat atcgacccaa gtaccgccac
     8581 ctaacaattc gttcaagccg agatcggctt cccggcctag agtcgatcga caagctcgag
     8641 tttctccata ataatgtgtg agtagttccc agataaggga attagggttc ctatagggtt
     8701 tcgctcatgt gttgagcata taagaaaccc ttagtatgta tttgtatttg taaaatactt
     8761 ctatcaataa aatttctaat tcctaaaacc aaaatccagt actaaaatcc agatcccccg
     8821 aattaattcg gcgttaattc agtacattaa aaacgtccgc aatgtgttat taagttgtct
     8881 aagcgtcaat ttgtttacac cacaatatat cctgccacca gccagccaac agctccccga
     8941 ccggcagctc ggcacaaaat caccactcga tacaggcagc ccatcagtcc gggacggcgt
     9001 cagcgggaga gccgttgtaa ggcggcagac tttgctcatg ttaccgatgc tattcggaag
     9061 aacggcaact aagctgccgg gtttgaaaca cggatgatct cgcggagggt agcatgttga
     9121 ttgtaacgat gacagagcgt tgctgcctgt gatcaattcg ggcacgaacc cagtggacat
     9181 aagcctgttc ggttcgtaag ctgtaatgca agtagcgtat gcgctcacgc aactggtcca
     9241 gaaccttgac cgaacgcagc ggtggtaacg gcgcagtggc ggttttcatg gcttgttatg
     9301 actgtttttt tggggtacag tctatgcctc gggcatccaa gcagcaagcg cgttacgccg
     9361 tgggtcgatg tttgatgtta tggagcagca acgatgttac gcagcagggc agtcgcccta
     9421 aaacaaagtt aaacatcatg ggggaagcgg tgatcgccga agtatcgact caactatcag
     9481 aggtagttgg cgtcatcgag cgccatctcg aaccgacgtt gctggccgta catttgtacg
     9541 gctccgcagt ggatggcggc ctgaagccac acagtgatat tgatttgctg gttacggtga
     9601 ccgtaaggct tgatgaaaca acgcggcgag ctttgatcaa cgaccttttg gaaacttcgg
     9661 cttcccctgg agagagcgag attctccgcg ctgtagaagt caccattgtt gtgcacgacg
     9721 acatcattcc gtggcgttat ccagctaagc gcgaactgca atttggagaa tggcagcgca
     9781 atgacattct tgcaggtatc ttcgagccag ccacgatcga cattgatctg gctatcttgc
     9841 tgacaaaagc aagagaacat agcgttgcct tggtaggtcc agcggcggag gaactctttg
     9901 atccggttcc tgaacaggat ctatttgagg cgctaaatga aaccttaacg ctatggaact
     9961 cgccgcccga ctgggctggc gatgagcgaa atgtagtgct tacgttgtcc cgcatttggt
    10021 acagcgcagt aaccggcaaa atcgcgccga aggatgtcgc tgccgactgg gcaatggagc
    10081 gcctgccggc ccagtatcag cccgtcatac ttgaagctag acaggcttat cttggacaag
    10141 aagaagatcg cttggcctcg cgcgcagatc agttggaaga atttgtccac tacgtgaaag
    10201 gcgagatcac caaggtagtc ggcaaataat gtctagctag aaattcgttc aagccgacgc
    10261 cgcttcgcgg cgcggcttaa ctcaagcgtt agatgcacta agcacataat tgctcacagc
    10321 caaactatca ggtcaagtct gcttttatta tttttaagcg tgcataataa gccctacaca
    10381 aattgggaga tatatcatgc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
    10441 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
    10501 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
    10561 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
    10621 tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
    10681 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
    10741 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
    10801 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
    10861 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
    10921 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
    10981 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
    11041 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
    11101 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc
    11161 gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg
    11221 agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt
    11281 gcggtatttc acaccgcata tggtgcactc tcagtacaat ctgctctgat gccgcatagt
    11341 taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc
    11401 gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca
    11461 agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg
    11521 cgcgaggcag ggtgccttga tgtgggcgcc ggcggtcgag tggcgacggc gcggcttgtc
    11581 cgcgccctgg tagattgcct ggccgtaggc cagccatttt tgagcggcca gcggccgcga
    11641 taggccgacg cgaagcggcg gggcgtaggg agcgcagcga ccgaagggta ggcgcttttt
    11701 gcagctcttc ggctgtgcgc tggccagaca gttatgcaca ggccaggcgg gttttaagag
    11761 ttttaataag ttttaaagag ttttaggcgg aaaaatcgcc ttttttctct tttatatcag
    11821 tcacttacat gtgtgaccgg ttcccaatgt acggctttgg gttcccaatg tacgggttcc
    11881 ggttcccaat gtacggcttt gggttcccaa tgtacgtgct atccacagga aagagacctt
    11941 ttcgaccttt ttcccctgct agggcaattt gccctagcat ctgctccgta cattaggaac
    12001 cggcggatgc ttcgccctcg atcaggttgc ggtagcgcat gactaggatc gggccagcct
    12061 gccccgcctc ctccttcaaa tcgtactccg gcaggtcatt tgacccgatc agcttgcgca
    12121 cggtgaaaca gaacttcttg aactctccgg cgctgccact gcgttcgtag atcgtcttga
    12181 acaaccatct ggcttctgcc ttgcctgcgg cgcggcgtgc caggcggtag agaaaacggc
    12241 cgatgccggg atcgatcaaa aagtaatcgg ggtgaaccgt cagcacgtcc gggttcttgc
    12301 cttctgtgat ctcgcggtac atccaatcag ctagctcgat ctcgatgtac tccggccgcc
    12361 cggtttcgct ctttacgatc ttgtagcggc taatcaaggc ttcaccctcg gataccgtca
    12421 ccaggcggcc gttcttggcc ttcttcgtac gctgcatggc aacgtgcgtg gtgtttaacc
    12481 gaatgcaggt ttctaccagg tcgtctttct gctttccgcc atcggctcgc cggcagaact
    12541 tgagtacgtc cgcaacgtgt ggacggaaca cgcggccggg cttgtctccc ttcccttccc
    12601 ggtatcggtt catggattcg gttagatggg aaaccgccat cagtaccagg tcgtaatccc
    12661 acacactggc catgccggcc ggccctgcgg aaacctctac gtgcccgtct ggaagctcgt
    12721 agcggatcac ctcgccagct cgtcggtcac gcttcgacag acggaaaacg gccacgtcca
    12781 tgatgctgcg actatcgcgg gtgcccacgt catagagcat cggaacgaaa aaatctggtt
    12841 gctcgtcgcc cttgggcggc ttcctaatcg acggcgcacc ggctgccggc ggttgccggg
    12901 attctttgcg gattcgatca gcggccgctt gccacgattc accggggcgt gcttctgcct
    12961 cgatgcgttg ccgctgggcg gcctgcgcgg ccttcaactt ctccaccagg tcatcaccca
    13021 gcgccgcgcc gatttgtacc gggccggatg gtttgcgacc gctcacgccg attcctcggg
    13081 cttgggggtt ccagtgccat tgcagggccg gcagacaacc cagccgctta cgcctggcca
    13141 accgcccgtt cctccacaca tggggcattc cacggcgtcg gtgcctggtt gttcttgatt
    13201 ttccatgccg cctcctttag ccgctaaaat tcatctactc atttattcat ttgctcattt
    13261 actctggtag ctgcgcgatg tattcagata gcagctcggt aatggtcttg ccttggcgta
    13321 ccgcgtacat cttcagcttg gtgtgatcct ccgccggcaa ctgaaagttg acccgcttca
    13381 tggctggcgt gtctgccagg ctggccaacg ttgcagcctt gctgctgcgt gcgctcggac
    13441 ggccggcact tagcgtgttt gtgcttttgc tcattttctc tttacctcat taactcaaat
    13501 gagttttgat ttaatttcag cggccagcgc ctggacctcg cgggcagcgt cgccctcggg
    13561 ttctgattca agaacggttg tgccggcggc ggcagtgcct gggtagctca cgcgctgcgt
    13621 gatacgggac tcaagaatgg gcagctcgta cccggccagc gcctcggcaa cctcaccgcc
    13681 gatgcgcgtg cctttgatcg cccgcgacac gacaaaggcc gcttgtagcc ttccatccgt
    13741 gacctcaatg cgctgcttaa ccagctccac caggtcggcg gtggcccata tgtcgtaagg
    13801 gcttggctgc accggaatca gcacgaagtc ggctgccttg atcgcggaca cagccaagtc
    13861 cgccgcctgg ggcgctccgt cgatcactac gaagtcgcgc cggccgatgg ccttcacgtc
    13921 gcggtcaatc gtcgggcggt cgatgccgac aacggttagc ggttgatctt cccgcacggc
    13981 cgcccaatcg cgggcactgc cctggggatc ggaatcgact aacagaacat cggccccggc
    14041 gagttgcagg gcgcgggcta gatgggttgc gatggtcgtc ttgcctgacc cgcctttctg
    14101 gttaagtaca gcgataacct tcatgcgttc cccttgcgta tttgtttatt tactcatcgc
    14161 atcatatacg cagcgaccgc atgacgcaag ctgttttact caaatacaca tcaccttttt
    14221 agacggcggc gctcggtttc ttcagcggcc aagctggccg gccaggccgc cagcttggca
    14281 tcagacaaac cggccaggat ttcatgcagc cgcacggttg agacgtgcgc gggcggctcg
    14341 aacacgtacc cggccgcgat catctccgcc tcgatctctt cggtaatgaa aaacggttcg
    14401 tcctggccgt cctggtgcgg tttcatgctt gttcctcttg gcgttcattc tcggcggccg
    14461 ccagggcgtc ggcctcggtc aatgcgtcct cacggaaggc accgcgccgc ctggcctcgg
    14521 tgggcgtcac ttcctcgctg cgctcaagtg cgcggtacag ggtcgagcga tgcacgccaa
    14581 gcagtgcagc cgcctctttc acggtgcggc cttcctggtc gatcagctcg cgggcgtgcg
    14641 cgatctgtgc cggggtgagg gtagggcggg ggccaaactt cacgcctcgg gccttggcgg
    14701 cctcgcgccc gctccgggtg cggtcgatga ttagggaacg ctcgaactcg gcaatgccgg
    14761 cgaacacggt caacaccatg cggccggccg gcgtggtggt gtcggcccac ggctctgcca
    14821 ggctacgcag gcccgcgccg gcctcctgga tgcgctcggc aatgtccagt aggtcgcggg
    14881 tgctgcgggc caggcggtct agcctggtca ctgtcacaac gtcgccaggg cgtaggtggt
    14941 caagcatcct ggccagctcc gggcggtcgc gcctggtgcc ggtgatcttc tcggaaaaca
    15001 gcttggtgca gccggccgcg tgcagttcgg cccgttggtt ggtcaagtcc tggtcgtcgg
    15061 tgctgacgcg ggcatagccc agcaggccag cggcggcgct cttgttcatg gcgtaatgtc
    15121 tccggttcta gtcgcaagta ttctacttta tgcgactaaa acacgcgaca agaaaacgcc
    15181 aggaaaaggg cagggcggca gcctgtcgcg taacttagga cttgtgcgac atgtcgtttt
    15241 cagaagacgg ctgcactgaa cgtcagaagc cgactgcact atagcagcgg aggggttgga
    15301 tcaaagtact
//
