LOCUS       sequence-449392-        8940 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression vector with VPS35 tagged with mScarlet.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8940)
  AUTHORS   Conway JRW, Joshi O, Kaivola J, Follain G, Gounis M, Kuhl D, Ivaska 
            J
  TITLE     Dynamic regulation of integrin beta1 phosphorylation supports 
            invasion of breast cancer cells.
  JOURNAL   Nat Cell Biol. 2025 May 26. doi: 10.1038/s41556-025-01663-4.
  PUBMED    40419795
REFERENCE   2  (bases 1 to 8940)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Cell 
            Biol. 2025 May 26. doi: 10.1038/s41556-025-01663-4."
COMMENT     Sequence Label: pEF.DEST51-VPS35-mScarlet
FEATURES             Location/Qualifiers
     source          1..8940
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(166..185)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        473..1651
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          704..1642
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1599..1619
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     primer_bind     1668..1687
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1668..1686
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    1718..1742
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             4177..4872
                     /codon_start=1
                     /product="bright monomeric red fluorescent protein evolved 
                     from a synthetic template (Bindels et al., 2016)"
                     /label=mScarlet
                     /translation="MVSKGEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFSWDILSPQFMYGSRAFTKHPADIPDYYKQSFPEGFKWERVMNFEDGG
                     AVTVTQDTSLEDGTLIYKVKLRGTNFPPDGPVMQKKTMGWEASTERLYPEDGVLKGDIK
                     MALRLKDGGRYLADFKTTYKAKKPVQMPGAYNVDRKLDITSHNEDYTVVEQYERSEGRH
                     STGGMDELYK"
     primer_bind     complement(4310..4328)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     4578..4597
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     protein_bind    complement(4888..4912)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             4938..4979
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             4989..5006
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(5029..5046)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5035..5259
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5305..5733
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5392..5411)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5602..5623
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5742..5762)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5747..6076
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5927..6062
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5989..6008
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        6124..6171
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             6190..6588
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     polyA_signal    6746..6867
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6783..6802)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6837..6856
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6916..6932)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6916..6932)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6929..6951)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6940..6956
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6964..6970,6971..6988,6989..6994))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7009..7030
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7147..7164)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7307..7895)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7387..7406)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8066..8857,8858..8926))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8689..8708
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(join(8927..8940,1..91))
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
       61 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg
      121 tcgacggatc gggagatctc ccgatcccct atggtgcact ctcagtacaa tctgctctga
      181 tgccgcatag ttaagccagt atctgctccc tgcttgtgtg ttggaggtcg ctgagtagtg
      241 cgcgagcaaa atttaagcta caacaaggca aggcttgacc gacaattgca tgaagaatct
      301 gcttagggtt aggcgttttg cgctgcttcg cgatgtacgg gccagatata cgcgttgaca
      361 ttgattattg actaggcttt tgcaaaaagc tttgcaaaga tggataaagt tttaaacaga
      421 gaggaatctt tgcagctaat ggaccttcta ggtcttgaaa ggagtgggaa ttggctccgg
      481 tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg ggggaggggt
      541 cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa gtgatgtcgt
      601 gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg cagtagtcgc
      661 cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg ccgtgtgtgg
      721 ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa ttacttccac
      781 ctggctgcag tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt gggagagttc
      841 gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct ggcctgggcg
      901 ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg ctttcgataa
      961 gtctctagcc atttaaaatt tttgatgacc tgctgcgacg ctttttttct ggcaagatag
     1021 tcttgtaaat gcgggccaag atctgcacac tggtatttcg gtttttgggg ccgcgggcgg
     1081 cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc gagcgcggcc
     1141 accgagaatc ggacgggggt agtctcaagc tggccggcct gctctggtgc ctggcctcgc
     1201 gccgccgtgt atcgccccgc cctgggcggc aaggctggcc cggtcggcac cagttgcgtg
     1261 agcggaaaga tggccgcttc ccggccctgc tgcagggagc tcaaaatgga ggacgcggcg
     1321 ctcgggagag cgggcgggtg agtcacccac acaaaggaaa agggcctttc cgtcctcagc
     1381 cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg attagttctc
     1441 gagcttttgg agtacgtcgt ctttaggttg gggggagggg ttttatgcga tggagtttcc
     1501 ccacactgag tgggtggaga ctgaagttag gccagcttgg cacttgatgt aattctcctt
     1561 ggaatttgcc ctttttgagt ttggatcttg gttcattctc aagcctcaga cagtggttca
     1621 aagttttttt cttccatttc aggtgtcgtg aggaattagc ttggtactaa tacgactcac
     1681 tatagggaga cccaagctgg ctaggtaagc ttgatcaaca agtttgtaca aaaaagcagg
     1741 ctggcgccgg aaccaattca gtcgactggt accggcacca tgcctacaac acagcagtcc
     1801 cctcaggatg agcaggaaaa gctcttggat gaagccatac aggctgtgaa ggtccagtca
     1861 ttccaaatga agagatgcct ggacaaaaac aagcttatgg atgctctaaa acatgcttct
     1921 aatatgcttg gtgaactccg gacttctatg ttatcaccaa agagttacta tgaactttat
     1981 atggccattt ctgatgaact gcactacttg gaggtctacc tgacagatga gtttgctaaa
     2041 ggaaggaaag tggcagatct ctacgaactt gtacagtatg ctggaaacat tatcccaagg
     2101 ctttaccttt tgatcacagt tggagttgta tatgtcaagt catttcctca gtccaggaag
     2161 gatattttga aagatttggt agaaatgtgc cgtggtgtgc aacatccctt gaggggtctg
     2221 tttcttcgaa attaccttct tcagtgtacc agaaatatct tacctgatga aggagagcca
     2281 acagatgaag aaacaactgg tgacatcagt gattccatgg attttgtact gctcaacttt
     2341 gcagaaatga acaagctctg ggtgcgaatg cagcatcagg gacatagccg agatagagaa
     2401 aaaagagaac gagaaagaca agaactgaga attttagtgg gaacaaattt ggtgcgcctc
     2461 agtcagttgg aaggtgtaaa tgtggaacgt tacaaacaga ttgttttgac tggcatattg
     2521 gagcaagttg taaactgtag ggatgctttg gctcaagaat atctcatgga gtgtattatt
     2581 caggttttcc ctgatgaatt tcacctccag actttgaatc cttttcttcg ggcctgtgct
     2641 gagttacacc agaatgtaaa tgtgaagaac ataatcattg ctttaattga tagattagct
     2701 ttatttgctc accgtgaaga tggacctgga atcccagcgg atattaaact ttttgatata
     2761 ttttcacagc aggtggctac agtgatacag tctagacaag acatgccttc agaggatgtt
     2821 gtatctttac aagtctctct gattaatctt gccatgaaat gttaccctga tcgtgtggac
     2881 tatgttgata aagttctaga aacaacagtg gagatattca ataagctcaa ccttgaacat
     2941 attgctacca gtagtgcagt ttcaaaggaa ctcaccagac ttttgaaaat accagttgac
     3001 acttacaaca atattttaac agtcttgaaa ttaaaacatt ttcacccact ctttgagtac
     3061 tttgactacg agtccagaaa gagcatgagt tgttatgtgc ttagtaatgt tctggattat
     3121 aacacagaaa ttgtctctca agaccaggtg gattccataa tgaatttggt atccacgttg
     3181 attcaagatc agccagatca acctgtagaa gaccctgatc cagaagattt tgctgatgag
     3241 cagagccttg tgggccgctt cattcatctg ctgcgctctg aggaccctga ccagcagtac
     3301 ttgattttga acacagcacg aaaacatttt ggagctggtg gaaatcagcg gattcgcttc
     3361 acactgccac ctttggtatt tgcagcttac cagctggctt ttcgatataa agagaattct
     3421 aaagtggatg acaaatggga aaagaaatgc cagaagattt tttcatttgc ccaccagact
     3481 atcagtgctt tgatcaaagc agagctggca gaattgccct taagactttt tcttcaagga
     3541 gcactagctg ctggggaaat tggttttgaa aatcatgaga cagtcgcata tgaattcatg
     3601 tcccaggcat tttctctgta tgaagatgaa atcagcgatt ccaaagcaca gctagctgcc
     3661 atcaccttga tcattggcac ttttgaaagg atgaagtgct tcagtgaaga gaatcatgaa
     3721 cctctgagga ctcagtgtgc ccttgctgca tccaaacttc taaagaaacc tgatcagggc
     3781 cgagctgtga gcacctgtgc acatctcttc tggtctggca gaaacacgga caaaaatggg
     3841 gaggagcttc acggaggcaa gagggtaatg gagtgcctaa aaaaagctct aaaaatagca
     3901 aatcagtgca tggacccctc tctacaagtg cagcttttta tagaaattct gaacagatat
     3961 atctattttt atgaaaagga aaatgatgcg gtaacaattc aggttttaaa ccagcttatc
     4021 caaaagattc gagaagacct cccgaatctt gaatccagtg aagaaacaga gcagattaac
     4081 aaacattttc ataacacact ggagcatttg cgcttgcggc gggaatcacc agaatccgag
     4141 gggccaattt atgaaggtct catccttggc ggatccatgg tgagcaaggg cgaggcagtg
     4201 atcaaggagt tcatgcggtt caaggtgcac atggagggct ccatgaacgg ccacgagttc
     4261 gagatcgagg gcgagggcga gggccgcccc tacgagggca cccagaccgc caagctgaag
     4321 gtgaccaagg gtggccccct gcccttctcc tgggacatcc tgtcccctca gttcatgtac
     4381 ggctccaggg ccttcaccaa gcaccccgcc gacatccccg actactataa gcagtccttc
     4441 cccgagggct tcaagtggga gcgcgtgatg aacttcgagg acggcggcgc cgtgaccgtg
     4501 acccaggaca cctccctgga ggacggcacc ctgatctaca aggtgaagct ccgcggcacc
     4561 aacttccctc ctgacggccc cgtaatgcag aagaagacaa tgggctggga agcgtccacc
     4621 gagcggttgt accccgagga cggcgtgctg aagggcgaca ttaagatggc cctgcgcctg
     4681 aaggacggcg gccgctacct ggcggacttc aagaccacct acaaggccaa gaagcccgtg
     4741 cagatgcccg gcgcctacaa cgtcgaccgc aagttggaca tcacctccca caacgaggac
     4801 tacaccgtgg tggaacagta cgaacgctcc gagggccgcc actccaccgg cggcatggac
     4861 gagctgtaca agtagtaact cgagtagacc cagctttctt gtacaaagtg gttgatctag
     4921 agggcccgcg gttcgaaggt aagcctatcc ctaaccctct cctcggtctc gattctacgc
     4981 gtaccggtca tcatcaccat caccattgag tttaaacccg ctgatcagcc tcgactgtgc
     5041 cttctagttg ccagccatct gttgtttgcc cctcccccgt gccttccttg accctggaag
     5101 gtgccactcc cactgtcctt tcctaataaa atgaggaaat tgcatcgcat tgtctgagta
     5161 ggtgtcattc tattctgggg ggtggggtgg ggcaggacag caagggggag gattgggaag
     5221 acaatagcag gcatgctggg gatgcggtgg gcgctatggc ttctgaggcg gaaagaacca
     5281 gctggggctc tagggggtat ccccacgcgc cctgtagcgg cgcattaagc gcggcgggtg
     5341 tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg
     5401 ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg
     5461 ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt
     5521 agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt
     5581 tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta
     5641 tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa
     5701 atgagctgat ttaacaaaaa tttaacgcga attaattctg tggaatgtgt gtcagttagg
     5761 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta
     5821 gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat
     5881 gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac
     5941 tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga
     6001 ggccgaggcc gcctctgcct ctgagctatt ccagaagtag tgaggaggct tttttggagg
     6061 cctaggcttt tgcaaaaagc tcccgggagc ttgtatatcc attttcggat ctgatcagca
     6121 cgtgttgaca attaatcatc ggcatagtat atcggcatag tataatacga caaggtgagg
     6181 aactaaacca tggccaagcc tttgtctcaa gaagaatcca ccctcattga aagagcaacg
     6241 gctacaatca acagcatccc catctctgaa gactacagcg tcgccagcgc agctctctct
     6301 agcgacggcc gcatcttcac tggtgtcaat gtatatcatt ttactggggg accttgtgca
     6361 gaactcgtgg tgctgggcac tgctgctgct gcggcagctg gcaacctgac ttgtatcgtc
     6421 gcgatcggaa atgagaacag gggcatcttg agcccctgcg gacggtgccg acaggtgctt
     6481 ctcgatctgc atcctgggat caaagccata gtgaaggaca gtgatggaca gccgacggca
     6541 gttgggattc gtgaattgct gccctctggt tatgtgtggg agggctaagc acttcgtggc
     6601 cgaggagcag gactgacacg tgctacgaga tttcgattcc accgccgcct tctatgaaag
     6661 gttgggcttc ggaatcgttt tccgggacgc cggctggatg atcctccagc gcggggatct
     6721 catgctggag ttcttcgccc accccaactt gtttattgca gcttataatg gttacaaata
     6781 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
     6841 tttgtccaaa ctcatcaatg tatcttatca tgtctgtata ccgtcgacct ctagctagag
     6901 cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc
     6961 acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta
     7021 actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca
     7081 gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc
     7141 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
     7201 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
     7261 gtgagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
     7321 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
     7381 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
     7441 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
     7501 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
     7561 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
     7621 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
     7681 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
     7741 ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
     7801 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
     7861 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
     7921 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
     7981 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
     8041 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
     8101 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
     8161 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac
     8221 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
     8281 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
     8341 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac
     8401 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
     8461 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
     8521 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
     8581 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
     8641 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc
     8701 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
     8761 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
     8821 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
     8881 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc
//
