LOCUS       sequence-449424-        4651 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry vector with a phosphatase-inactive PTP-PEST mutant 
            (D199A,C231S).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4651)
  AUTHORS   Conway JRW, Joshi O, Kaivola J, Follain G, Gounis M, Kuhl D, Ivaska 
            J
  TITLE     Dynamic regulation of integrin beta1 phosphorylation supports 
            invasion of breast cancer cells.
  JOURNAL   Nat Cell Biol. 2025 May 26. doi: 10.1038/s41556-025-01663-4.
  PUBMED    40419795
REFERENCE   2  (bases 1 to 4651)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Cell 
            Biol. 2025 May 26. doi: 10.1038/s41556-025-01663-4."
COMMENT     Sequence Label: pENTR2b-PTPN12(D199A,C231S)
FEATURES             Location/Qualifiers
     source          1..4651
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      103..189
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      281..308
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     primer_bind     316..339
                     /label=pENTR-F
                     /note="pENTR vectors, forward primer"
     protein_bind    358..457
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2880..2979)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(3091..3110)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             3102..3911
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(3169..3188)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      4001..4589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4490..4509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgctagca tggatctcgg
       61 ggacgtctaa ctactaagcg agagtaggga actgccaggc atcaaataaa acgaaaggct
      121 cagtcggaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctcctgagt
      181 aggacaaatc cgccgggagc ggatttgaac gttgtgaagc aacggcccgg agggtggcgg
      241 gcaggacgcc cgccataaac tgccaggcat caaactaagc agaaggccat cctgacggat
      301 ggcctttttg cgtttctaca aactcttcct gttagttagt tacttaagct cgggccccaa
      361 ataatgattt tattttgact gatagtgacc tgttcgttgc aacaaattga taagcaatgc
      421 ttttttataa tgccaacttt gtacaaaaaa gcaggctggc gccggaacca attcagtcga
      481 ctggatccgg taccgaggag atctgccgcc gcgatcgcca tggagcaagt ggagatcctg
      541 aggaaattca tccagagggt ccaggccatg aagagtcctg accacaatgg ggaggacaac
      601 ttcgcccggg acttcatgcg gttaagaaga ttgtctacca aatatagaac agaaaagata
      661 tatcccacag ccactggaga aaaagaagaa aatgttaaaa agaacagata caaggacata
      721 ctgccatttg atcacagccg agttaaattg acattaaaga ctccttcaca agattcagac
      781 tatatcaatg caaattttat aaagggcgtc tatgggccaa aagcatatgt agcaactcaa
      841 ggacctttag caaatacagt aatagatttt tggaggatga tatgggagta taatgttgtg
      901 atcattgtaa tggcctgccg agaatttgag atgggaagga aaaaatgtga gcgctattgg
      961 cctttgtatg gagaagaccc cataacgttt gcaccattta aaatttcttg tgaggatgaa
     1021 caagcaagaa cagactactt catcaggaca ctcttacttg aatttcaaaa tgaatctcgt
     1081 aggctgtatc agtttcatta tgtgaactgg ccagcccatg atgttccttc atcatttgat
     1141 tctattctgg acatgataag cttaatgagg aaatatcaag aacatgaaga tgttcctatt
     1201 tgtattcata gcagtgcagg ctgtggaaga acaggtgcca tttgtgccat agattatacg
     1261 tggaatttac taaaagctgg gaaaatacca gaggaattta atgtatttaa tttaatacaa
     1321 gaaatgagaa cacaaaggca ttctgcagta caaacaaagg agcaatatga acttgttcat
     1381 agagctattg cccaactgtt tgaaaaacag ctacaactat atgaaattca tggagctcag
     1441 aaaattgctg atggagtgaa tgaaattaac actgaaaaca tggtcagctc catagagcct
     1501 gaaaaacaag attctcctcc tccaaaacca ccaaggaccc gcagttgcct tgttgaaggg
     1561 gatgctaaag aagaaatact gcagccaccg gaacctcatc cagtgccacc catcttgaca
     1621 ccttctcccc cttcagcttt tccaacagtc actactgtgt ggcaggacaa tgatagatac
     1681 catccaaagc cagtgttgca tatggtttca tcagaacaac attcagcaga cctcaacaga
     1741 aactatagta aatcaacaga acttccaggg aaaaatgaat caacaattga acagatagat
     1801 aaaaaattgg aacgaaattt aagttttgag attaagaagg tccctctcca agagggacca
     1861 aaaagttttg atgggaacac acttttgaat aggggacatg caattaaaat taaatctgct
     1921 tcaccttgta tagctgataa aatctctaag ccacaggaat taagttcaga tctaaatgtc
     1981 ggtgatactt cccagaattc ttgtgtggac tgcagtgtaa cacaatcaaa caaagtttca
     2041 gttactccac cagaagaatc ccagaattca gacacacctc caaggccaga ccgcttgcct
     2101 cttgatgaga aaggacatgt aacgtggtca tttcatggac ctgaaaatgc catacccata
     2161 cctgatttat ctgaaggcaa ttcctcagat atcaactatc aaactaggaa aactgtgagt
     2221 ttaacaccaa gtcctacaac acaagttgaa acacctgatc ttgtggatca tgataacact
     2281 tcaccactct tcagaacacc cctcagtttt actaatccac ttcactctga tgactcagac
     2341 tcagatgaaa gaaactctga tggtgctgtg acccagaata aaactaatat ttcaacagca
     2401 agtgccacag tttctgctgc cactagtact gaaagcattt ctactaggaa agtattgcca
     2461 atgtccattg ctagacataa tatagcagga acaacacatt caggtgctga aaaagatgtt
     2521 gatgttagtg aagattcacc tcctccccta cctgaaagaa ctcctgaatc gtttgtgtta
     2581 gcaagtgaac ataatacacc tgtaagatcg gaatggagtg aacttcaaag tcaggaacga
     2641 tctgaacaaa aaaagtctga aggcttgata acctctgaaa atgagaaatg tgatcatcca
     2701 gcgggaggta ttcactatga aatgtgcata gaatgtccac ctactttcag tgacaagaga
     2761 gaacaaatat cagaaaatcc aacagaagcc acagatattg gttttggtaa tcgatgtgga
     2821 aaacccaaag gaccaagaga tccaccttca tgcggccgct gataactcga gatatctaga
     2881 cccagctttc ttgtacaaag ttggcattat aagaaagcat tgcttatcaa tttgttgcaa
     2941 cgaacaggtc actatcagtc aaaataaaat cattatttgc catccagctg cagctctggc
     3001 ccgtgtctca aaatctctga tgttacattg cacaagataa aaatatatca tcatgaacaa
     3061 taaaactgtc tgcttacata aacagtaata caaggggtgt tatgagccat attcaacggg
     3121 aaacgtcgag gccgcgatta aattccaaca tggatgctga tttatatggg tataaatggg
     3181 ctcgcgataa tgtcgggcaa tcaggtgcga caatctatcg cttgtatggg aagcccgatg
     3241 cgccagagtt gtttctgaaa catggcaaag gtagcgttgc caatgatgtt acagatgaga
     3301 tggtcagact aaactggctg acggaattta tgcctcttcc gaccatcaag cattttatcc
     3361 gtactcctga tgatgcatgg ttactcacca ctgcgatccc cggaaaaaca gcattccagg
     3421 tattagaaga atatcctgat tcaggtgaaa atattgttga tgcgctggca gtgttcctgc
     3481 gccggttgca ttcgattcct gtttgtaatt gtccttttaa cagcgatcgc gtatttcgtc
     3541 tcgctcaggc gcaatcacga atgaataacg gtttggttga tgcgagtgat tttgatgacg
     3601 agcgtaatgg ctggcctgtt gaacaagtct ggaaagaaat gcataaactt ttgccattct
     3661 caccggattc agtcgtcact catggtgatt tctcacttga taaccttatt tttgacgagg
     3721 ggaaattaat aggttgtatt gatgttggac gagtcggaat cgcagaccga taccaggatc
     3781 ttgccatcct atggaactgc ctcggtgagt tttctccttc attacagaaa cggctttttc
     3841 aaaaatatgg tattgataat cctgatatga ataaattgca gtttcatttg atgctcgatg
     3901 agtttttcta atcagaattg gttaattggt tgtaacatta ttcagattgg gccccgttcc
     3961 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     4021 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     4081 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     4141 atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     4201 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     4261 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     4321 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
     4381 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
     4441 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
     4501 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
     4561 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
     4621 tggccttttg ctggcctttt gctcacatgt t
//
