LOCUS       sequence-449450-       11228 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for Wnt3a and BFP expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11228)
  AUTHORS   Yamada T, Trentesaux C, Brunger JM, Xiao Y, Stevens AJ, Martyn I, 
            Kasparek P, Shroff NP, Aguilar A, Bruneau BG, Boffelli D, Klein OD, 
            Lim WA
  TITLE     Synthetic organizer cells guide development via spatial and 
            biochemical instructions.
  JOURNAL   Cell. 2024 Dec 11:S0092-8674(24)01323-0. doi: 
            10.1016/j.cell.2024.11.017.
  PUBMED    39706189
REFERENCE   2  (bases 1 to 11228)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2024 
            Dec 11:S0092-8674(24)01323-0. doi: 10.1016/j.cell.2024.11.017."
COMMENT     Sequence Label: aTY65_pTRE_Wnt3a_IRES_tagBFP2_WPRE
FEATURES             Location/Qualifiers
     source          1..11228
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        34..383
                     /label=TRE3GV promoter
                     /note="3rd-generation Tet-responsive promoter that can be 
                     activated by binding of Tet-On(R) 3G, optimized for 
                     retroviral and lentiviral vectors"
     protein_bind    42..60
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    78..96
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    114..132
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    150..168
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    186..204
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    222..240
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    258..276
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     322..346
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     misc_feature    1514..2087
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(1681..1698)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     1908..1927
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             2117..2815
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="SELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKVV
                     EGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTAT
                     QDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALKL
                     VGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYCD
                     LPSKLGHKLN"
     misc_feature    2830..3418
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2883..2903)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3301..3312)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(3440..3456)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3441..3457)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             3547..3780
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(3805..3823)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(3806..3823)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(4113..4132)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(4346..4365)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4465..4487
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(4525..4543)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     rep_origin      complement(4722..5310)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4802..4821)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     complement(5524..5543)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             5535..6350
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             5535..6350
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(5608..5627)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     6525..6542
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(6612..6632)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6617..6946
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6797..6932
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          8080..8145
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             8275..8295
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    8720..8854
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8757..8776)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8811..8830
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             9022..9655
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    9702..9827
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    10324..10557
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10742..10786
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    11071..11188
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ctgatgagac gaacggccct ttcgtcttca ctcgagttta ctccctatca gtgatagaga
       61 acgtatgaag agtttactcc ctatcagtga tagagaacgt atgcagactt tactccctat
      121 cagtgataga gaacgtataa ggagtttact ccctatcagt gatagagaac gtatgaccag
      181 tttactccct atcagtgata gagaacgtat ctacagttta ctccctatca gtgatagaga
      241 acgtatatcc agtttactcc ctatcagtga tagagaacgt atgtcgaggt aggcgtgtac
      301 ggtgggcgcc tataaaagca gagctcgttt agtgaaccgt cagatcgcct ggagcaattc
      361 cacaacactt ttgtcttata cttacgcgtc tgcaggatat caagcttgcg gtaccgcggg
      421 cccgggatct atggctcctc tcggatacct cttagtgctc tgcagcctga agcaggctct
      481 gggcagctac ccgatctggt ggtccttggc tgtgggaccc cagtactcct ctctgagcac
      541 tcagcccatt ctctgtgcca gcatcccagg cctggtaccg aagcagctgc gcttctgcag
      601 gaactacgtg gagatcatgc ccagcgtggc tgagggtgtc aaagcgggca tccaggagtg
      661 ccagcaccag ttccgaggcc ggcgttggaa ctgcaccacc gtcagcaaca gcctggccat
      721 ctttggccct gttctggaca aagccacccg ggagtcagcc tttgtccatg ccatcgcctc
      781 cgctggagta gctttcgcag tgacacgctc ctgtgcagag ggatcagctg ctatctgtgg
      841 gtgcagcagc cgcctccagg gctccccagg cgagggctgg aagtggggcg gctgtagtga
      901 ggacattgaa tttggaggaa tggtatctcg ggagtttgcc gatgccaggg agaaccggcc
      961 ggatgcccgc tctgccatga accgtcacaa caatgaggct gggcgccagg ccatcgccag
     1021 tcacatgcac ctcaagtgca aatgccacgg gctatctggc agctgtgaag tgaagacctg
     1081 ctggtggtcg cagccggact tccgcaccat cggggatttc ctcaaggaca agtatgacag
     1141 tgcctcggag atggtggtag agaaacaccg agagtctcgt ggctgggtgg agacactgag
     1201 gccacgttac acgtacttca aggtgccgac agaacgcgac ctggtctact acgaggcctc
     1261 acccaacttc tgcgaaccta accccgaaac cggctccttc gggacgcgtg accgcacctg
     1321 caatgtgagc tcgcatggca tagatgggtg cgacctgttg tgctgcgggc gcgggcataa
     1381 cgcgcgcact gagcgacgga gggagaaatg ccactgtgtt ttccattggt gctgctacgt
     1441 cagctgccag gagtgcacac gtgtctatga cgtgcacacc tgcaagggtt cttaagatcc
     1501 ttgacttgcg gcccccctct ccctcccccc cccctaacgt tactggccga agccgcttgg
     1561 aataaggccg gtgtgcgttt gtctatatgt tattttccac catattgccg tcttttggca
     1621 atgtgagggc ccggaaacct ggccctgtct tcttgacgag cattcctagg ggtctttccc
     1681 ctctcgccaa aggaatgcaa ggtctgttga atgtcgtgaa ggaagcagtt cctctggaag
     1741 cttcttgaag acaaacaacg tctgtagcga ccctttgcag gcagcggaac cccccacctg
     1801 gcgacaggtg cctctgcggc caaaagccac gtgtataaga tacacctgca aaggcggcac
     1861 aaccccagtg ccacgttgtg agttggatag ttgtggaaag agtcaaatgg ctctcctcaa
     1921 gcgtattcaa caaggggctg aaggatgccc agaaggtacc ccattgtatg ggatctgatc
     1981 tggggcctcg gtgcacatgc tttacatgtg tttagtcgag gttaaaaaaa cgtctaggcc
     2041 ccccgaacca cggggacgtg gttttccttt gaaaaacacg atgataaatg agcgctggcg
     2101 gaccaccatg ggatccagcg agctgattaa ggagaacatg cacatgaagc tgtacatgga
     2161 gggcaccgtg gacaaccatc acttcaagtg cacatccgag ggcgaaggca agccctacga
     2221 gggcacccag accatgagaa tcaaggtggt cgagggcggc cctctcccct tcgccttcga
     2281 catcctggct actagcttcc tctacggcag caagaccttc atcaaccaca cccagggcat
     2341 ccccgacttc ttcaagcagt ccttccctga gggcttcaca tgggagagag tcaccacata
     2401 cgaagacggg ggcgtgctga ccgctaccca ggacaccagc ctccaggacg gctgcctcat
     2461 ctacaacgtc aagatcagag gggtgaactt cacatccaac ggccctgtga tgcagaagaa
     2521 aacactcggc tgggaggcct tcaccgaaac gctgtacccc gctgacggcg gcctggaagg
     2581 cagaaacgac atggccctga agctcgtggg cgggagccat ctgatcgcaa acatcaagac
     2641 cacatataga tccaagaaac ccgctaagaa cctcaagatg cctggcgtct actatgtgga
     2701 ctacagactg gaaagaatca aggaggccaa caacgaaacc tacgtcgagc agcacgaggt
     2761 ggcagtggcc agatactgcg acctccctag caaactgggg cacaagctta attaatggcg
     2821 cttatcgata atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac
     2881 tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt
     2941 gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat
     3001 gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca
     3061 acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc
     3121 cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg
     3181 gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaaatcatc gtcctttcct
     3241 tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct
     3301 tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt
     3361 ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcat
     3421 gctgcgtctc aagcatacac gataccgtcg acctcgaggg aattaattcg agctcggtac
     3481 ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg
     3541 ggggactgga agggctaatt cactcccaac gaagacaaga tctgcttttt gcttgtactg
     3601 ggtttctctg gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac
     3661 tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt
     3721 gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca
     3781 gcatctagaa ttaattccgt gtattctata gtgtcaccta aatcgtatgt gtatgataca
     3841 taaggttatg tattaattgt agccgcgttc taacgacaat atgtacaagc ctaattgtgt
     3901 agcatctggc ttactgaagc agaccctatc atctctctcg taaactgccg tcagagtcgg
     3961 tttggttgga cgaaccttct gagtttctgg taacgccgtc ccgcacccgg aaatggtcag
     4021 cgaaccaatc agcagggtca tcgctagcca gatcctctac gccggacgca tcgtggccgg
     4081 catcaccggc gccacaggtg cggttgctgg cgcctatatc gccgacatca ccgatgggga
     4141 agatcgggct cgccacttcg ggctcatgag cgcttgtttc ggcgtgggta tggtggcagg
     4201 ccccgtggcc gggggactgt tgggcgccat ctccttgcat gcaccattcc ttgcggcggc
     4261 ggtgctcaac ggcctcaacc tactactggg ctgcttccta atgcaggagt cgcataaggg
     4321 agagcgtcga atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagc
     4381 cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg
     4441 cttacagaca agctgtgacc atctccggga gctgcatgtg tcagaggttt tcaccgtcat
     4501 caccgaaacg cgcgagatga aagggcctcg tgatacgcct atttttatag gttaatgtca
     4561 tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg ccgagcggcc
     4621 gcacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
     4681 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
     4741 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
     4801 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
     4861 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
     4921 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
     4981 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
     5041 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
     5101 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
     5161 agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
     5221 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
     5281 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
     5341 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgactga tccttcaact
     5401 cagcaaaagt tcgatttatt caacaaagcc acgttgtgtc tcaaaatctc tgatgttaca
     5461 ttgcacaaga taaaaatata tcatcatgaa caataaaact gtctgcttac ataaacagta
     5521 atacaagggg tgttatgagc catattcaac gggaaacgtc ttgctccagg ccgcgattaa
     5581 attccaacat ggatgctgat ttatatgggt ataaatgggc tcgcgataat gtcgggcaat
     5641 caggtgcgac aatctatcga ttgtatggga agcccgatgc gccagagttg tttctgaaac
     5701 atggcaaagg tagcgttgcc aatgatgtta cagatgagat ggtcagacta aactggctga
     5761 cggaatttat gcctcttccg accatcaagc attttatccg tactcctgat gatgcatggt
     5821 tactcaccac tgcgatcccc gggaaaacag cattccaggt attagaagaa tatcctgatt
     5881 caggtgaaaa tattgttgat gcgctggcag tgttcctgcg ccggttgcat tcgattcctg
     5941 tttgtaattg tccttttaac agcgatcgcg tatttcgcct cgctcaggcg caatcacgaa
     6001 tgaataacgg tttggttgat gcgagtgatt ttgatgacga gcgtaatggc tggcctgttg
     6061 aacaagtctg gaaagaaatg cataagcttt tgccattctc accggattca gtcgtcactc
     6121 atggtgattt ctcacttgat aaccttattt ttgacgaggg gaaattaata ggttgtattg
     6181 atgttggacg agtcggaatc gcagaccgat accaggatct tgccatccta tggaactgcc
     6241 tcggtgagtt ttctccttca ttacagaaac ggctttttca aaaatatggt attgataatc
     6301 ctgatatgaa taaattgcag tttcatttga tgctcgatga gtttttctaa tcagaattgg
     6361 ttaattggtt gtaacacgcg gccgccaatt ttttacggtt cctggccttt tgctggcctt
     6421 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
     6481 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
     6541 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
     6601 aatgcagctg tggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccagcagg
     6661 cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg
     6721 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc
     6781 gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca
     6841 tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt
     6901 ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc ttggacacaa
     6961 gacaggcttg cgagatatgt ttgagaatac cactttatcc cgcgtcaggg agaggcagtg
     7021 cgtaaaaaga cgcggactca tgtgaaatac tggtttttag tgcgccagat ctctataatc
     7081 tcgcgcaacc tattttcccc tcgaacactt tttaagccgt agataaacag gctgggacac
     7141 ttcacatgag cgaaaaatac atcgtcacct gggacatgtt gcagatccat gcacgtaaac
     7201 tcgcaagccg actgatgcct tctgaacaat ggaaaggcat tattgccgta agccgtggcg
     7261 gtctgtaccg ggtgcgttac tggcgcgtga actgggtatt cgtcatgtcg ataccgtttg
     7321 tatttccagc tacgatcacg acaaccagcg cgagcttaaa gtgctgaaac gcgcagaagg
     7381 cgatggcgaa ggcttcatcg ttattgatga cctggtggat accggtggta ctgcggttgc
     7441 gattcgtgaa atgtatccaa aagcgcactt tgtcaccatc ttcgcaaaac cggctggtcg
     7501 tccgctggtt gatgactatg ttgttgatat cccgcaagat acctggattg aacagccgtg
     7561 ggatatgggc gtcgtattcg tcccgccaat ctccggtcgc taatcttttc aacgcctggc
     7621 actgccgggc gttgttcttt ttaacttcag gcgggttaca atagtttcca gtaagtattc
     7681 tggaggctgc atccatgaca caggcaaacc tgagcgaaac cctgttcaaa ccccgcttta
     7741 aacatcctga aacctcgacg ctagtccgcc gctttaatca cggcgcacaa ccgcctgtgc
     7801 agtcggccct tgatggtaaa accatccctc actggtatcg catgattaac cgtctgatgt
     7861 ggatctggcg cggcattgac ccacgcgaaa tcctcgacgt ccaggcacgt attgtgatga
     7921 gcgatgccga acgtaccgac gatgatttat acgatacggt gattggctac cgtggcggca
     7981 actggattta tgagtgggcc ccggatcttt gtgaaggaac cttacttctg tggtgtgaca
     8041 taattggaca aactacctac agagatttaa agctctaagg taaatataaa atttttaagt
     8101 gtataatgtg ttaaactact gattctaatt gtttgtgtat tttagattcc aacctatgga
     8161 actgatgaat gggagcagtg gtggaatgcc tttaatgagg aaaacctgtt ttgctcagaa
     8221 gaaatgccat ctagtgatga tgaggctact gctgactctc aacattctac tcctccaaaa
     8281 aagaagagaa aggtagaaga ccccaaggac tttccttcag aattgctaag ttttttgagt
     8341 catgctgtgt ttagtaatag aactcttgct tgctttgcta tttacaccac aaaggaaaaa
     8401 gctgcactgc tatacaagaa aattatggaa aaatattctg taacctttat aagtaggcat
     8461 aacagttata atcataacat actgtttttt cttactccac acaggcatag agtgtctgct
     8521 attaataact atgctcaaaa attgtgtacc tttagctttt taatttgtaa aggggttaat
     8581 aaggaatatt tgatgtatag tgccttgact agagatcata atcagccata ccacatttgt
     8641 agaggtttta cttgctttaa aaaacctccc acacctcccc ctgaacctga aacataaaat
     8701 gaatgcaatt gttgttgtta acttgtttat tgcagcttat aatggttaca aataaagcaa
     8761 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc
     8821 caaactcatc aatgtatctt atcatgtctg gatcaactgg ataactcaag ctaaccaaaa
     8881 tcatcccaaa cttcccaccc cataccctat taccactgcc aattacctgt ggtttcattt
     8941 actctaaacc tgtgattcct ctgaattatt ttcattttaa agaaattgta tttgttaaat
     9001 atgtactaca aacttagtag ttggaagggc taattcactc ccaaagaaga caagatatcc
     9061 ttgatctgtg gatctaccac acacaaggct acttccctga ttagcagaac tacacaccag
     9121 ggccaggggt cagatatcca ctgacctttg gatggtgcta caagctagta ccagttgagc
     9181 cagataaggt agaagaggcc aataaaggag agaacaccag cttgttacac cctgtgagcc
     9241 tgcatgggat ggatgacccg gagagagaag tgttagagtg gaggtttgac agccgcctag
     9301 catttcatca cgtggcccga gagctgcatc cggagtactt caagaactgc tgatatcgag
     9361 cttgctacaa gggactttcc gctggggact ttccagggag gcgtggcctg ggcgggactg
     9421 gggagtggcg agccctcaga tcctgcatat aagcagctgc tttttgcctg tactgggact
     9481 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
     9541 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
     9601 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     9661 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     9721 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     9781 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     9841 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     9901 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     9961 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
    10021 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
    10081 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
    10141 aaagtaagac caccgcacag caagcggccg gccgctgatc ttcagacctg gaggaggaga
    10201 tatgagggac aattggagaa gtgaattata taaatataaa gtagtaaaaa ttgaaccatt
    10261 aggagtagca cccaccaagg caaagagaag agtggtgcag agagaaaaaa gagcagtggg
    10321 aataggagct ttgttccttg ggttcttggg agcagcagga agcactatgg gcgcagcgtc
    10381 aatgacgctg acggtacagg ccagacaatt attgtctggt atagtgcagc agcagaacaa
    10441 tttgctgagg gctattgagg cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa
    10501 gcagctccag gcaagaatcc tggctgtgga aagataccta aaggatcaac agctcctggg
    10561 gatttggggt tgctctggaa aactcatttg caccactgct gtgccttgga atgctagttg
    10621 gagtaataaa tctctggaac agatttggaa tcacacgacc tggatggagt gggacagaga
    10681 aattaacaat tacacaagct taatacactc cttaattgaa gaatcgcaaa accagcaaga
    10741 aaagaatgaa caagaattat tggaattaga taaatgggca agtttgtgga attggtttaa
    10801 cataacaaat tggctgtggt atataaaatt attcataatg atagtaggag gcttggtagg
    10861 tttaagaata gtttttgctg tactttctat agtgaataga gttaggcagg gatattcacc
    10921 attatcgttt cagacccacc tcccaacccc gaggggaccc gacaggcccg aaggaataga
    10981 agaagaaggt ggagagagag acagagacag atccattcga ttagtgaacg gatctcgacg
    11041 gtatcgccaa atggcagtat tcatccacaa ttttaaaaga aaagggggga ttggggggta
    11101 cagtgcaggg gaaagaatag tagacataat agcaacagac atacaaacta aagaattaca
    11161 aaaacaaatt acaaaaattc aaaattttcg ggtttattac agggacagca gagatccagt
    11221 ttggccct
//
