LOCUS       sequence-449511-       12475 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12475)
  AUTHORS   Guo W, Keckesova Z, Donaher JL, Shibue T, Tischler V, Reinhardt F, 
            Itzkovitz S, Noske A, Zurrer-Hardi U, Bell G, Tam WL, Mani SA, van 
            Oudenaarden A, Weinberg RA
  TITLE     Slug and Sox9 cooperatively determine the mammary stem cell state.
  JOURNAL   Cell. 2012 Mar 2;148(5):1015-28.
  PUBMED    22385965
REFERENCE   2  (bases 1 to 12475)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell."; 
            date: "2012-03-2"; volume: "148(5)"; pages: "1015-28"
COMMENT     Sequence Label: pWPXL-Sox9
FEATURES             Location/Qualifiers
     source          1..12475
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(830..850)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        835..1164
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      1015..1150
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          2298..2363
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             2493..2513
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    2938..3072
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2975..2994)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3029..3048
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             3240..3873
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    3920..4045
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    4538..4771
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             4956..5000
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        5383..6561
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          5614..6552
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     6509..6529
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     misc_feature    6609..6726
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    6816..6840
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(9476..9500)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    9544..10132
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(9597..9617)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(10015..10026)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    complement(10276..10309)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     LTR             10329..10509
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(10534..10552)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(10535..10552)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(10842..10861)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(11075..11094)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     11194..11216
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(11254..11272)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        11340..11444
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(11445..11513,11514..12305)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(11663..11682)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctgtg gaatgtgtgt
      841 cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat
      901 ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg
      961 caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg
     1021 cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt
     1081 tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt
     1141 tttggaggcc taggcttttg caaaaagctt ggacacaaga caggcttgcg agatatgttt
     1201 gagaatacca ctttatcccg cgtcagggag aggcagtgcg taaaaagacg cggactcatg
     1261 tgaaatactg gtttttagtg cgccagatct ctataatctc gcgcaaccta ttttcccctc
     1321 gaacactttt taagccgtag ataaacaggc tgggacactt cacatgagcg aaaaatacat
     1381 cgtcacctgg gacatgttgc agatccatgc acgtaaactc gcaagccgac tgatgccttc
     1441 tgaacaatgg aaaggcatta ttgccgtaag ccgtggcggt ctgtaccggg tgcgttactg
     1501 gcgcgtgaac tgggtattcg tcatgtcgat accgtttgta tttccagcta cgatcacgac
     1561 aaccagcgcg agcttaaagt gctgaaacgc gcagaaggcg atggcgaagg cttcatcgtt
     1621 attgatgacc tggtggatac cggtggtact gcggttgcga ttcgtgaaat gtatccaaaa
     1681 gcgcactttg tcaccatctt cgcaaaaccg gctggtcgtc cgctggttga tgactatgtt
     1741 gttgatatcc cgcaagatac ctggattgaa cagccgtggg atatgggcgt cgtattcgtc
     1801 ccgccaatct ccggtcgcta atcttttcaa cgcctggcac tgccgggcgt tgttcttttt
     1861 aacttcaggc gggttacaat agtttccagt aagtattctg gaggctgcat ccatgacaca
     1921 ggcaaacctg agcgaaaccc tgttcaaacc ccgctttaaa catcctgaaa cctcgacgct
     1981 agtccgccgc tttaatcacg gcgcacaacc gcctgtgcag tcggcccttg atggtaaaac
     2041 catccctcac tggtatcgca tgattaaccg tctgatgtgg atctggcgcg gcattgaccc
     2101 acgcgaaatc ctcgacgtcc aggcacgtat tgtgatgagc gatgccgaac gtaccgacga
     2161 tgatttatac gatacggtga ttggctaccg tggcggcaac tggatttatg agtgggcccc
     2221 ggatctttgt gaaggaacct tacttctgtg gtgtgacata attggacaaa ctacctacag
     2281 agatttaaag ctctaaggta aatataaaat ttttaagtgt ataatgtgtt aaactactga
     2341 ttctaattgt ttgtgtattt tagattccaa cctatggaac tgatgaatgg gagcagtggt
     2401 ggaatgcctt taatgaggaa aacctgtttt gctcagaaga aatgccatct agtgatgatg
     2461 aggctactgc tgactctcaa cattctactc ctccaaaaaa gaagagaaag gtagaagacc
     2521 ccaaggactt tccttcagaa ttgctaagtt ttttgagtca tgctgtgttt agtaatagaa
     2581 ctcttgcttg ctttgctatt tacaccacaa aggaaaaagc tgcactgcta tacaagaaaa
     2641 ttatggaaaa atattctgta acctttataa gtaggcataa cagttataat cataacatac
     2701 tgttttttct tactccacac aggcatagag tgtctgctat taataactat gctcaaaaat
     2761 tgtgtacctt tagcttttta atttgtaaag gggttaataa ggaatatttg atgtatagtg
     2821 ccttgactag agatcataat cagccatacc acatttgtag aggttttact tgctttaaaa
     2881 aacctcccac acctccccct gaacctgaaa cataaaatga atgcaattgt tgttgttaac
     2941 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat
     3001 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat
     3061 catgtctgga tcaactggat aactcaagct aaccaaaatc atcccaaact tcccacccca
     3121 taccctatta ccactgccaa ttacctgtgg tttcatttac tctaaacctg tgattcctct
     3181 gaattatttt cattttaaag aaattgtatt tgttaaatat gtactacaaa cttagtagtt
     3241 ggaagggcta attcactccc aaagaagaca agatatcctt gatctgtgga tctaccacac
     3301 acaaggctac ttccctgatt agcagaacta cacaccaggg ccaggggtca gatatccact
     3361 gacctttgga tggtgctaca agctagtacc agttgagcca gataaggtag aagaggccaa
     3421 taaaggagag aacaccagct tgttacaccc tgtgagcctg catgggatgg atgacccgga
     3481 gagagaagtg ttagagtgga ggtttgacag ccgcctagca tttcatcacg tggcccgaga
     3541 gctgcatccg gagtacttca agaactgctg atatcgagct tgctacaagg gactttccgc
     3601 tggggacttt ccagggaggc gtggcctggg cgggactggg gagtggcgag ccctcagatc
     3661 ctgcatataa gcagctgctt tttgcctgta ctgggtctct ctggttagac cagatctgag
     3721 cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt
     3781 gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca
     3841 gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc ccgaacaggg acttgaaagc
     3901 gaaagggaaa ccagaggagc tctctcgacg caggactcgg cttgctgaag cgcgcacggc
     3961 aagaggcgag gggcggcgac tggtgagtac gccaaaaatt ttgactagcg gaggctagaa
     4021 ggagagagat gggtgcgaga gcgtcagtat taagcggggg agaattagat cgcgatggga
     4081 aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa ttaaaacata tagtatgggc
     4141 aagcagggag ctagaacgat tcgcagttaa tcctggcctg ttagaaacat cagaaggctg
     4201 tagacaaata ctgggacagc tacaaccatc ccttcagaca ggatcagaag aacttagatc
     4261 attatataat acagtagcaa ccctctattg tgtgcatcaa aggatagaga taaaagacac
     4321 caaggaagct ttagacaaga tagaggaaga gcaaaacaaa agtaagacca ccgcacagca
     4381 agcggccgct gatcttcaga cctggaggag gagatatgag ggacaattgg agaagtgaat
     4441 tatataaata taaagtagta aaaattgaac cattaggagt agcacccacc aaggcaaaga
     4501 gaagagtggt gcagagagaa aaaagagcag tgggaatagg agctttgttc cttgggttct
     4561 tgggagcagc aggaagcact atgggcgcag cgtcaatgac gctgacggta caggccagac
     4621 aattattgtc tggtatagtg cagcagcaga acaatttgct gagggctatt gaggcgcaac
     4681 agcatctgtt gcaactcaca gtctggggca tcaagcagct ccaggcaaga atcctggctg
     4741 tggaaagata cctaaaggat caacagctcc tggggatttg gggttgctct ggaaaactca
     4801 tttgcaccac tgctgtgcct tggaatgcta gttggagtaa taaatctctg gaacagattt
     4861 ggaatcacac gacctggatg gagtgggaca gagaaattaa caattacaca agcttaatac
     4921 actccttaat tgaagaatcg caaaaccagc aagaaaagaa tgaacaagaa ttattggaat
     4981 tagataaatg ggcaagtttg tggaattggt ttaacataac aaattggctg tggtatataa
     5041 aattattcat aatgatagta ggaggcttgg taggtttaag aatagttttt gctgtacttt
     5101 ctatagtgaa tagagttagg cagggatatt caccattatc gtttcagacc cacctcccaa
     5161 ccccgagggg acccgacagg cccgaaggaa tagaagaaga aggtggagag agagacagag
     5221 acagatccat tcgattagtg aacggatctc gacggtatcg atcacgagac tagcctcgac
     5281 catcgatgtc gacgataagc tttgcaaaga tggataaagt tttaaacaga gaggaatctt
     5341 tgcagctaat ggaccttcta ggtcttgaaa ggagtgggaa ttggctccgg tgcccgtcag
     5401 tgggcagagc gcacatcgcc cacagtcccc gagaagttgg ggggaggggt cggcaattga
     5461 accggtgcct agagaaggtg gcgcggggta aactgggaaa gtgatgtcgt gtactggctc
     5521 cgcctttttc ccgagggtgg gggagaaccg tatataagtg cagtagtcgc cgtgaacgtt
     5581 ctttttcgca acgggtttgc cgccagaaca caggtaagtg ccgtgtgtgg ttcccgcggg
     5641 cctggcctct ttacgggtta tggcccttgc gtgccttgaa ttacttccac ctggctgcag
     5701 tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt gggagagttc gaggccttgc
     5761 gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct ggcctgggcg ctggggccgc
     5821 cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg ctttcgataa gtctctagcc
     5881 atttaaaatt tttgatgacc tgctgcgacg ctttttttct ggcaagatag tcttgtaaat
     5941 gcgggccaag atctgcacac tggtatttcg gtttttgggg ccgcgggcgg cgacggggcc
     6001 cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc gagcgcggcc accgagaatc
     6061 ggacgggggt agtctcaagc tggccggcct gctctggtgc ctggcctcgc gccgccgtgt
     6121 atcgccccgc cctgggcggc aaggctggcc cggtcggcac cagttgcgtg agcggaaaga
     6181 tggccgcttc ccggccctgc tgcagggagc tcaaaatgga ggacgcggcg ctcgggagag
     6241 cgggcgggtg agtcacccac acaaaggaaa agggcctttc cgtcctcagc cgtcgcttca
     6301 tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg attagttctc gagcttttgg
     6361 agtacgtcgt ctttaggttg gggggagggg ttttatgcga tggagtttcc ccacactgag
     6421 tgggtggaga ctgaagttag gccagcttgg cacttgatgt aattctcctt ggaatttgcc
     6481 ctttttgagt ttggatcttg gttcattctc aagcctcaga cagtggttca aagttttttt
     6541 cttccatttc aggtgtcgtg aggaatttcg acatttaaat ttaattaatc tcgacggtat
     6601 cggttaactt ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta
     6661 gacataatag caacagacat acaaactaaa gaattacaaa aacaaattac aaaaattcaa
     6721 aattttatcg atcacgagac tagcctcgag gtttaaacta cgggatccag gcctaagctt
     6781 acgcgtccca gtgtggtgga attctgcaga tatcaacaag tttgtacaaa aaagcaggct
     6841 ggtaccggtc cggaattccc gggatatcgt cgacccacgc gtccgagcgc tgctcggaac
     6901 tgcctggaaa cttctgtggg agcgacaact ttaccagttt cagtccagga acttttcttt
     6961 gcaagagaga cgaggtgcaa gtggccccgg tttcgttctc tgttttccct ccctcctcct
     7021 ccgctccgac tcgccttccc cgggtttaga gccggcagct gagacccgcc acccagcgcc
     7081 tctgctaagt gcccgccgcc gcagcccggt gacgcgccaa cctccccggg agccgttcgc
     7141 tcggcgtccg cgtccgggca gctgagggaa gaggagcccc agccgccgcg gcttctcgcc
     7201 tttcccggcc acccgccccc tgccccgggc tcgcgtatga atctcctgga ccccttcatg
     7261 aagatgaccg acgagcagga gaagggcctg tctggcgccc ccagccccac catgtcggag
     7321 gactcggctg gttcgccctg tccctcgggc tccggctcgg acacggagaa cacccggccc
     7381 caggagaaca ccttccccaa gggcgagccg gatctgaaga aggagagcga ggaagataag
     7441 ttccccgtgt gcatccgcga ggcggtcagc caggtgctga agggctacga ctggacgctg
     7501 gtgcccatgc ccgtgcgcgt caacggctcc agcaagaaca agccacacgt caagcgaccc
     7561 atgaacgcct tcatggtgtg ggcgcaggct gcgcgcagga agctggcaga ccagtacccg
     7621 catctgcaca acgcggagct cagcaagact ctgggcaagc tctggaggct gctgaacgag
     7681 agcgagaaga gacccttcgt ggaggaggcg gagcggctgc gcgtgcagca caagaaagac
     7741 caccccgatt acaagtacca gccccggcgg aggaagtcgg tgaagaacgg acaagcggag
     7801 gccgaagagg ccacggaaca gactcacatc tctcctaatg ctatcttcaa ggcgctgcaa
     7861 gccgactccc cacattcctc ctccggcatg agtgaggtgc actccccggg cgagcactct
     7921 gggcaatctc agggtccgcc gaccccaccc accactccca aaaccgacgt gcaagctggc
     7981 aaagttgatc tgaagcgaga ggggcgccct ctggcagagg ggggcagaca gccccccatc
     8041 gacttccgcg acgtggacat cggtgaactg agcagcgacg tcatctccaa cattgagacc
     8101 ttcgacgtca atgagtttga ccaatacttg ccacccaacg gccacccagg ggttccggcc
     8161 acccacggcc aggtcaccta cactggcagt tacggcatca gcagcaccgc acccacccct
     8221 gcgaccgcgg gccacgtgtg gatgtcgaag cagcaggcgc cgccccctcc tccgcagcag
     8281 cctccgcagg ccccgcaagc cccacaggcg cctccgcagc agcaagcacc cccgcagcag
     8341 ccgcaggcac cccagcagca gcaggcacac acgctcacca cgctgagcag cgagccaggc
     8401 cagtcccagc gaacgcacat caagacggag cagctgagcc ccagccacta cagcgagcag
     8461 cagcagcact ccccgcaaca gatctcctac agccccttca accttcctca ctacagcccc
     8521 tcctacccgc ccatcacccg ctcgcaatac gactacgctg accatcagaa ctccggctcc
     8581 tactacagtc acgcagccgg ccagggctca gggctctact ccaccttcac ttacatgaac
     8641 cccgcgcagc gccccatgta cacccccatc gctgacacct ccggggtccc ttccatcccg
     8701 cagacccaca gcccgcagca ctgggaacaa ccagtctaca cacagctcac cagaccctga
     8761 gaagagaaaa gctatggtga cagagctgat tctttttttt ttttttttaa agaagaaaag
     8821 aaagaaacga aaagaaaaag ctgaagaaat caagaaccaa ttgaaattcc tttggacact
     8881 tttttttttg tctatcgtta atttttaaag acatgtaaag gaaggtaacg attgctggga
     8941 ttccaggaga gagactttaa gactttgtct gagctcatga caacatattg caaatggccg
     9001 ggccactcgt ggccagacgg acagcactcc tggccagatg gacccaccag tatcagcgag
     9061 gaggggcttg tctccttcag agttaacatg gaggacgatt ggagaatctc cctgcctgtt
     9121 tggactttgt aattattttt tagccgtaat taaagaaaaa aaaagtcctc tgtgaggaat
     9181 attctctatt ttaaatactt ttagtatgta ctgtgtatga ctcattacca ttttgagggg
     9241 atttatacat attttagata aaaaaagtta aatgctctta tttttccaac agcttaacta
     9301 ctcctagttc aagagtgtcc cttagccttc cttgcaacca gagtattttt gtacagattt
     9361 gctttctcct aaacaaacaa acaaaaacaa aaacaaaaaa aacaaaaaaa aatgaaaaaa
     9421 aaaaaaaaag ggcggccgct ctagagtatc cctcgagggg cccaagctta cgcgtaccca
     9481 gctttcttgt acaaagtggt tgatatccag cacagtggtc gacgataaaa ctagtcatat
     9541 gataatcaac ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt
     9601 gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc
     9661 cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag
     9721 ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc
     9781 actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc
     9841 cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg
     9901 ctgttgggca ctgacaattc cgtggtgttg tcggggaagc tgacgtcctt tccatggctg
     9961 ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc
    10021 ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt
    10081 cttcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc gcatcggtac
    10141 gtatggccag gtacctttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt
    10201 tttaaaagaa aaggggggac tggaagggct aattcactcc caacgaagac aagatgggat
    10261 caattcacca tgggaataac ttcgtatagc atacattata cgaagttatg ctgctttttg
    10321 cttgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag
    10381 ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc
    10441 gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa
    10501 tctctagcag catctagaat taattccgtg tattctatag tgtcacctaa atcgtatgtg
    10561 tatgatacat aaggttatgt attaattgta gccgcgttct aacgacaata tgtacaagcc
    10621 taattgtgta gcatctggct tactgaagca gaccctatca tctctctcgt aaactgccgt
    10681 cagagtcggt ttggttggac gaaccttctg agtttctggt aacgccgtcc cgcacccgga
    10741 aatggtcagc gaaccaatca gcagggtcat cgctagccag atcctctacg ccggacgcat
    10801 cgtggccggc atcaccggcg ccacaggtgc ggttgctggc gcctatatcg ccgacatcac
    10861 cgatggggaa gatcgggctc gccacttcgg gctcatgagc gcttgtttcg gcgtgggtat
    10921 ggtggcaggc cccgtggccg ggggactgtt gggcgccatc tccttgcatg caccattcct
    10981 tgcggcggcg gtgctcaacg gcctcaacct actactgggc tgcttcctaa tgcaggagtc
    11041 gcataaggga gagcgtcgaa tggtgcactc tcagtacaat ctgctctgat gccgcatagt
    11101 taagccagcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc
    11161 cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt
    11221 caccgtcatc accgaaacgc gcgagacgaa agggcctcgt gatacgccta tttttatagg
    11281 ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc
    11341 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
    11401 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt
    11461 tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag
    11521 aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg
    11581 aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa
    11641 tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc
    11701 aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag
    11761 tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa
    11821 ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc
    11881 taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg
    11941 agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa
    12001 caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa
    12061 tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg
    12121 gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag
    12181 cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg
    12241 caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt
    12301 ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt
    12361 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac
    12421 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttc
//
