LOCUS       sequence-449591-        9594 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  DjCas13d protein for RNA targeting in piggyBac backbone.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9594)
  AUTHORS   Wei J, Lotfy P, Faizi K, Baungaard S, Gibson E, Wang E, Slabodkin H,
            Kinnaman E, Chandrasekaran S, Kitano H, Durrant MG, Duffy CV, Pawluk
            A, Hsu PD, Konermann S
  TITLE     Deep learning and CRISPR-Cas13d ortholog discovery for optimized RNA
            targeting.
  JOURNAL   Cell Syst. 2023 Dec 20;14(12):1087-1102.e13. doi: 
            10.1016/j.cels.2023.11.006. Epub 2023 Dec 12.
  PUBMED    38091991
REFERENCE   2  (bases 1 to 9594)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cels.2023.11.006"; journalName: "Cell Syst"; date: 
            "2023-12-20- 20"; volume: "14"; issue: "12"; pages: "1087-1102.e13"
COMMENT     Sequence Label: PB_EF1a-DjCas13d-msfGFP-Blast
FEATURES             Location/Qualifiers
     source          1..9594
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    877..898
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(913..918,919..936,937..943)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    951..967
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     956..978
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     975..991
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     975..991
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        1012..1030
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     repeat_region   1188..1222
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     promoter        1547..2725
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          1778..2716
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     2673..2693
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             2744..2764
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6170..6190
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6200..6226
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             6236..6292
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             6293..6691
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    6707..7295
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6760..6780)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7178..7189)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(7465..7484)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     repeat_region   7666..7728
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(7794..7813)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7795..7813)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7820..7837)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7820..7836)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7829..7851)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7978..8433
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8065..8084)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8275..8296
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8459..8563
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8564..8632,8633..9424)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8782..8801)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
      841 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
      901 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
      961 ataacaattt cacacaggaa acagctatga ccatgattac gccaagctcg aaattaaccc
     1021 tcactaaagg gaacaaaagc tggtacctcg cgcgacttgg tttgccattc tttagcgcgc
     1081 gtcgcgtcac acagcttggc cacaatgtgg tttttgtcaa acgaagattc tatgacgtgt
     1141 ttaaagttta ggtcgagtaa agcgcaaatc ttttgtttga tgattaaccc tagaaagata
     1201 gtctgcgtaa aattgacgca tgcattcttg aaatattgct ctctctttct aaatagcgcg
     1261 aatccgtcgc tgtgcattta ggacatctca gtcgccgctt ggagctcccg tgaggcgtgc
     1321 ttgtcaatgc ggtaagtgtc actgattttg aactataacg accgcgtgag tcaaaatgac
     1381 gcatgattat cttttacgtg acttttaaga tttaactcat acgataatta tattgttatt
     1441 tcatgttcta cttacgtgat aacttattat atatatattt tcttgttata gatatcggaa
     1501 tctttgcagc taatggacct tctaggtctt gaaaggagtg ggaattggct ccggtgcccg
     1561 tcagtgggca gagcgcacat cgcccacagt ccccgagaag ttggggggag gggtcggcaa
     1621 ttgaaccggt gcctagagaa ggtggcgcgg ggtaaactgg gaaagtgatg tcgtgtactg
     1681 gctccgcctt tttcccgagg gtgggggaga accgtatata agtgcagtag tcgccgtgaa
     1741 cgttcttttt cgcaacgggt ttgccgccag aacacaggta agtgccgtgt gtggttcccg
     1801 cgggcctggc ctctttacgg gttatggccc ttgcgtgcct tgaattactt ccacctggct
     1861 gcagtacgtg attcttgatc ccgagcttcg ggttggaagt gggtgggaga gttcgaggcc
     1921 ttgcgcttaa ggagcccctt cgcctcgtgc ttgagttgag gcctggcctg ggcgctgggg
     1981 ccgccgcgtg cgaatctggt ggcaccttcg cgcctgtctc gctgctttcg ataagtctct
     2041 agccatttaa aatttttgat gacctgctgc gacgcttttt ttctggcaag atagtcttgt
     2101 aaatgcgggc caagatctgc acactggtat ttcggttttt ggggccgcgg gcggcgacgg
     2161 ggcccgtgcg tcccagcgca catgttcggc gaggcggggc ctgcgagcgc ggccaccgag
     2221 aatcggacgg gggtagtctc aagctggccg gcctgctctg gtgcctggcc tcgcgccgcc
     2281 gtgtatcgcc ccgccctggg cggcaaggct ggcccggtcg gcaccagttg cgtgagcgga
     2341 aagatggccg cttcccggcc ctgctgcagg gagctcaaaa tggaggacgc ggcgctcggg
     2401 agagcgggcg ggtgagtcac ccacacaaag gaaaagggcc tttccgtcct cagccgtcgc
     2461 ttcatgtgac tccacggagt accgggcgcc gtccaggcac ctcgattagt tctcgagctt
     2521 ttggagtacg tcgtctttag gttgggggga ggggttttat gcgatggagt ttccccacac
     2581 tgagtgggtg gagactgaag ttaggccagc ttggcacttg atgtaattct ccttggaatt
     2641 tgcccttttt gagtttggat cttggttcat tctcaagcct cagacagtgg ttcaaagttt
     2701 ttttcttcca tttcaggtgt cgtgacgtac ggccaccatg agccccaaga agaagagaaa
     2761 ggtggaggcc agcaagaagc agaagagcaa gaaaaccgtg agcaagacca gcggcctgaa
     2821 ggaggccctg agcgtgcagg gcaccgtgat catgaccagc ttcggcaagg gcaacatggc
     2881 caacctgagc tacaagatcc ccagcagcca gaagccccag aacctgaaca gcagcgccgg
     2941 cctgaagaac gtggaggtga gcggcaagaa gatcaagttc cagggcaggc accccaagat
     3001 cgccaccacc gacaaccccc tgttcaagcc ccagcccggc atggacctgc tgtgcctgaa
     3061 ggacaagctg gagatgcact acttcggcaa gaccttcgac gacaacatcc acatccagct
     3121 gatctaccag atcctggaca tcgagaagat cctggccgtg cacgtgaaca acatcgtgtt
     3181 caccctggac aacgtgctgc acccccagaa ggaggagctg accgaggact tcatcggcgc
     3241 cggcggctgg aggatcaacc tggactacca gaccctgagg ggccagacca acaagtacga
     3301 caggttcaag aactacatta agaggaagga gctgctgtac ttcggcgagg ccttctacca
     3361 cgagaacgag aggaggtacg aggaggacat cttcgccatc ctgaccctgc tgagcgccct
     3421 gaggcagttc tgcttccaca gcgacctgag cagcgacgag agcgaccacg tgaacagctt
     3481 ctggctgtac cagctggagg accagctgag cgacgagttc aaggagacac tgagcatcct
     3541 gtgggaggag gtgaccgaga ggatcgacag cgagttcctg aaaaccaaca ccgtgaacct
     3601 gcacatcctg tgccacgtgt tccccaagga gagcaaggag acaatcgtga gggcctacta
     3661 cgagttcctg atcaagaaga gcttcaagaa catgggcttc agcatcaaga agctgaggga
     3721 gatcatgctg gagcagagcg acctgaagag cttcaaggag gacaagtaca acagcgtgag
     3781 ggccaagctg tacaagctgt tcgacttcat catcacctac tactacgacc accacgcctt
     3841 cgagaaggag gccctggtga gcagcctgag gagcagcctg accgaggaga acaaggagga
     3901 gatctatatc aaaaccgcca ggaccctggc cagcgccctg ggcgccgact tcaagaaggc
     3961 cgccgccgac gtgaacgcca agaacatcag ggactaccag aagaaggcca acgactacag
     4021 gatcagcttc gaggacatta agatcggcaa caccggcatc ggctacttca gcgagctgat
     4081 ctacatgctg accctgctgc tggacggcaa ggagatcaac gacctgctga ccaccctgat
     4141 caacaagttc gacaacatca tcagcttcat cgacatcctg aagaagctga acctggagtt
     4201 caagttcaag cccgagtacg ccgacttctt caacatgacc aactgcaggt acaccctgga
     4261 ggagctgaga gtgatcaaca gcatcgccag gatgcagaag cccagcgccg acgccaggaa
     4321 gatcatgtac agggacgccc tgaggatcct gggcatggac aacaggcccg acgaggagat
     4381 cgacagggag ctggagagga ccatgcccgt gggcgccgac ggcaagttca tcaagggcaa
     4441 gcagggcttc aggaacttca tcgccagcaa cgtgatcgag agcagcaggt tccactacct
     4501 ggtgaggtac aacaaccccc acaagaccag gaccctggtg aagaacccca acgtggtgaa
     4561 gttcgtgctg gagggcatcc ccgagacaca gatcaagagg tacttcgacg tgtgcaaggg
     4621 ccaggagatc ccccccacca gcgacaagag cgcccagatc gacgtgctgg ccaggatcat
     4681 cagcagcgtg gactacaaga tcttcgagga cgtgccccag agcgccaaga tcaacaagga
     4741 cgaccccagc aggaacttca gcgacgccct gaagaagcag aggtaccagg ccatcgtgag
     4801 cctgtacctg accgtgatgt acctgatcac caagaacctg gtgtacgtga acagcaggta
     4861 cgtgatcgcc ttccactgcc tggagaggga cgccttcctg cacggcgtga ccctgcccaa
     4921 gatgaacaag aagatcgtgt acagccagct gaccacccac ctgctgaccg acaagaacta
     4981 caccacctac ggccacctga agaaccagaa gggccacagg aagtggtacg tgctggtgaa
     5041 gaacaacctg cagaacagcg acatcaccgc cgtgagcagc ttcaggaaca tcgtggccca
     5101 catcagcgtg gtgaggaaca gcaacgagta catcagcggc atcggcgagc tgcacagcta
     5161 cttcgagctg taccactacc tggtgcagag catgatcgcc aagaacaact ggtacgacac
     5221 cagccaccag cccaagaccg ccgagtacct gaacaacctg aagaagcacc acacctactg
     5281 caaggacttc gtgaaggcct actgcatccc cttcggctac gtggtgccca ggtacaagaa
     5341 cctgaccatc aacgagctgt tcgacaggaa caaccccaac cccgagccca aggaggaggt
     5401 gggaggaggt ggaagcggag gaggaggaag cggaggagga ggtagcgtga gtaaaggtga
     5461 agaactcttc actggagtag tgcccattct ggtagagctt gatggagatg taaatggaca
     5521 taaattctcc gtcaggggcg aaggcgaagg ggacgccacg aatggtaagc tgactctgaa
     5581 attcatctgt acgacgggca aactgcccgt cccatggcct acactcgtaa cgaccctcac
     5641 ctacggcgtg caatgctttt ctcgatatcc cgaccacatg aaacagcatg actttttcaa
     5701 gtctgcaatg cctgaaggtt atgttcaaga aaggaccatc agctttaagg atgatggtac
     5761 atataaaacc cgagccgagg ttaaatttga aggggacact ctggttaatc gaattgaact
     5821 gaaaggtatt gattttaagg aggacggtaa catactgggg cacaagttgg agtacaactt
     5881 taacagccat aatgtgtata ttaccgctga taagcagaaa aatgggataa aggccaactt
     5941 taagatccga cataatgtcg aagatggtag tgttcaactg gctgatcatt accaacaaaa
     6001 tacgcccatc ggagatggac ctgtactctt gcctgacaat cattatctct ccacgcaatc
     6061 aaagctttcc aaggacccaa acgaaaagag agatcacatg gtccttctgg aatttgtgac
     6121 tgccgcaggc atcactctcg gtatggatga gctgtacaag ggatccggac ctaagaaaaa
     6181 gaggaaggtg gcggccgctt acccatacga tgttccagat tacgctatcg atggcgcaac
     6241 aaacttctct ctgctgaaac aagccggaga tgtcgaagag aatcctggac cgatggccaa
     6301 gcctttgtct caagaagaat ccaccctcat tgaaagagca acggctacaa tcaacagcat
     6361 ccccatctct gaagactaca gcgtcgccag cgcagctctc tctagcgacg gccgcatctt
     6421 cactggtgtc aatgtatatc attttactgg gggaccttgt gcagaactcg tggtgctggg
     6481 cactgctgct gctgcggcag ctggcaacct gacttgtatc gtcgcgatcg gaaatgagaa
     6541 caggggcatc ttgagcccct gcggacggtg ccgacaggtg cttctcgatc tgcatcctgg
     6601 gatcaaagcc atagtgaagg acagtgatgg acagccgacg gcagttggga ttcgtgaatt
     6661 gctgccctct ggttatgtgt gggagggcta aacgcgttaa gtcgacaatc aacctctgga
     6721 ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg
     6781 tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt
     6841 ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag
     6901 gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc
     6961 caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga
     7021 actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa
     7081 ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct gtgttgccac
     7141 ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct
     7201 tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca
     7261 gacgagtcgg atttcccttt gggccgcctc cccgcgtcga ctttaagacc aatgacttac
     7321 aaggcataat cagccatacc acatttgtag aggttttact tgctttaaaa aacctcccac
     7381 acctccccct gaacctgaaa cataaaatga atgcaattgt tgttgttaac ttgtttattg
     7441 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat gctagctttt
     7501 gttactttat agaagaaatt ttgagttttt gttttttttt aataaataaa taaacataaa
     7561 taaattgttt gttgaattta ttattagtat gtaagtgtaa atataataaa acttaatatc
     7621 tattcaaatt aataaataaa cctcgatata cagaccgata aaacacatgc gtcaatttta
     7681 cgcatgatta tctttaacgt acgtcacaat atgattatct ttctagggtt aaataatagt
     7741 ttctaatttt tttattattc agcctgctgt cgtgaatacc gagctccaat tcgccctata
     7801 gtgagtcgta ttacaattca ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg
     7861 gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg
     7921 aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatgggacg
     7981 cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta
     8041 cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt
     8101 tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg
     8161 ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat
     8221 cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac
     8281 tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag
     8341 ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg
     8401 cgaattttaa caaaatatta acgcttacaa tttaggtggc acttttcggg gaaatgtgcg
     8461 cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca
     8521 ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt
     8581 ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga
     8641 aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga
     8701 actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat
     8761 gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca
     8821 agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt
     8881 cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac
     8941 catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct
     9001 aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga
     9061 gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac
     9121 aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat
     9181 agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg
     9241 ctggtttatt gctgataaat ctggagccgg tgagcgtggg tcccgcggta tcattgcagc
     9301 actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc
     9361 aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg
     9421 gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta
     9481 atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg
     9541 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttc
//
