LOCUS       sequence-449599-        8956 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expressing METTL17-LYRMut-FLAG.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8956)
  AUTHORS   Ast T, Itoh Y, Sadre S, McCoy JG, Namkoong G, Wengrod JC, Chicherin 
            I, Joshi PR, Kamenski P, Suess DLM, Amunts A, Mootha VK
  TITLE     METTL17 is an Fe-S cluster checkpoint for mitochondrial translation.
  JOURNAL   Mol Cell. 2024 Jan 18;84(2):359-374.e8. doi: 
            10.1016/j.molcel.2023.12.016. Epub 2024 Jan 9.
  PUBMED    38199006
REFERENCE   2  (bases 1 to 8956)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2023.12.016"; journalName: "Mol Cell"; date: 
            "2024-01-18- 18"; volume: "84"; issue: "2"; pages: "359-374.e8"
COMMENT     Sequence Label: pLYS6-METTL17-LYRMut-FLAG
FEATURES             Location/Qualifiers
     source          1..8956
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     363..382
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     LTR             587..820
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    892..1013
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(929..948)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     983..1002
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      1053..1188
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(1208..1227)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1209..1227)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1237..1254)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1237..1253)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1246..1268)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      1395..1850
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1482..1501)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1692..1713
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        1876..1980
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1981..2049,2050..2841)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2199..2218)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3012..3600
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3501..3520
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3754..3771
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    3888..3909
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(3924..3929,3930..3947,3948..3954)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3962..3978
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3967..3989
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     3986..4002
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3986..4002
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     4021..4041
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        4023..4041
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        4069..4295
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             4296..4476
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    4523..4648
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    5141..5374
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             5559..5603
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     primer_bind     5901..5921
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     promoter        6210..6413
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     6364..6384
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             7846..7911
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     misc_feature    7956..8073
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     primer_bind     8565..8584
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             join(8646..8956,1..493)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(8709..8728)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
ORIGIN
        1 tctcctgtca tctcaccttg ctcctgccga gaaagtatcc atcatggctg atgcaatgcg
       61 gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga aacatcgcat
      121 cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc tggacgaaga
      181 gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc aaggcgcgca tgcccgacgg
      241 cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg
      301 ccgcttttct ggattcatcg actgtggccg gctgggtgtg gcggaccgct atcaggacat
      361 agcgttggct acccgtgata ttgctgaaga gcttggcggc gaatgggctg accgcttcct
      421 cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc gccttcttga
      481 cgagttcttc tgaggggatc cgtcgacctg cagccaagct ctttaagacc aatgacttac
      541 aaggcagctg tagatcttag ccacttttta aaagaaaagg ggggactgga agggctaatt
      601 cactcccaac gaagacaaga tctgcttttt gcttgtactg ggtctctctg gttagaccag
      661 atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc
      721 ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga
      781 tccctcagac ccttttagtc agtgtggaaa atctctagca gtagtagttc atgtcatctt
      841 attattcagt atttataact tgcaaagaaa tgaatatcag agagtgagag gaacttgttt
      901 attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca
      961 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc
     1021 tggctctagc tatcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg
     1081 cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct
     1141 cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta gggacgtacc
     1201 caattcgccc tatagtgagt cgtattacgc gcgctcactg gccgtcgttt tacaacgtcg
     1261 tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc
     1321 cagctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct
     1381 gaatggcgaa tgggacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac
     1441 gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc
     1501 ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt
     1561 agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg
     1621 ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac
     1681 gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta
     1741 ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat
     1801 ttaacaaaaa tttaacgcga attttaacaa aatattaacg cttacaattt aggtggcact
     1861 tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg
     1921 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt
     1981 atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct
     2041 gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca
     2101 cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc
     2161 gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc
     2221 cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg
     2281 gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta
     2341 tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc
     2401 ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt
     2461 gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg
     2521 cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct
     2581 tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc
     2641 tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct
     2701 cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac
     2761 acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc
     2821 tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat
     2881 ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg
     2941 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
     3001 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
     3061 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
     3121 gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta
     3181 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
     3241 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
     3301 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     3361 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
     3421 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
     3481 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     3541 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
     3601 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
     3661 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
     3721 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
     3781 gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg
     3841 cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag
     3901 ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga
     3961 attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta cgccaagcgc
     4021 gcaattaacc ctcactaaag ggaacaaaag ctggagctgc aagcttaatg tagtcttatg
     4081 caatactctt gtagtcttgc aacatggtaa cgatgagtta gcaacatgcc ttacaaggag
     4141 agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg gtacgatcgt gccttattag
     4201 gaaggcaaca gacgggtctg acatggattg gacgaaccac tgaattgccg cattgcagag
     4261 atattgtatt taagtgccta gctcgataca taaacgggtc tctctggtta gaccagatct
     4321 gagcctggga gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc
     4381 cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc
     4441 tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca gggacttgaa
     4501 agcgaaaggg aaaccagagg agctctctcg acgcaggact cggcttgctg aagcgcgcac
     4561 ggcaagaggc gaggggcggc gactggtgag tacgccaaaa attttgacta gcggaggcta
     4621 gaaggagaga gatgggtgcg agagcgtcag tattaagcgg gggagaatta gatcgcgatg
     4681 ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat aaattaaaac atatagtatg
     4741 ggcaagcagg gagctagaac gattcgcagt taatcctggc ctgttagaaa catcagaagg
     4801 ctgtagacaa atactgggac agctacaacc atcccttcag acaggatcag aagaacttag
     4861 atcattatat aatacagtag caaccctcta ttgtgtgcat caaaggatag agataaaaga
     4921 caccaaggaa gctttagaca agatagagga agagcaaaac aaaagtaaga ccaccgcaca
     4981 gcaagcggcc gctgatcttc agacctggag gaggagatat gagggacaat tggagaagtg
     5041 aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc accaaggcaa
     5101 agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg ttccttgggt
     5161 tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg gtacaggcca
     5221 gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct attgaggcgc
     5281 aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca agaatcctgg
     5341 ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc tctggaaaac
     5401 tcatttgcac cactgctgtg ccttggaatg ctagttggag taataaatct ctggaacaga
     5461 tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac acaagcttaa
     5521 tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa gaattattgg
     5581 aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg ctgtggtata
     5641 taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt tttgctgtac
     5701 tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag acccacctcc
     5761 caaccccgag gggacccgac aggcccgaag gaatagaaga agaaggtgga gagagagaca
     5821 gagacagatc cattcgatta gtgaacggat ctcgacggta tcgatcacga gactagcctc
     5881 gagcggccgc ccccttcacc gagggcctat ttcccatgat tccttcatat ttgcatatac
     5941 gatacaaggc tgttagagag ataattggaa ttaatttgac tgtaaacaca aagatattag
     6001 tacaaaatac gtgacgtaga aagtaataat ttcttgggta gtttgcagtt ttaaaattat
     6061 gttttaaaat ggactatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat
     6121 ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
     6181 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
     6241 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
     6301 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
     6361 tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctggtttag
     6421 tgaaccgtca gatccgctag catggcggcg gcgttgaaat gcttgttgac tctgggtagg
     6481 tggtgtcctg gcttgggagt tgcaccacag gcacgagcac tcgcagcctt ggttcctggg
     6541 gtcacgcaag tcgacaataa gtctgggttt ctccagaaaa ggccccaccg gcaacatccc
     6601 ggtatcctga agctgccgca cgtccgactc ccgcaggcac tcgcgaatgg agcgcaattg
     6661 ctgctgcttg gtagcgctgg tcctacaatg gaaaatcaag tgcaaacctt gaccagctac
     6721 ctttggtcaa ggcatctccc agttgagcct gaagagcttc agagacgcgc caggcacctt
     6781 gaaaaaaagt tcttggagaa ccctgatttg agtcagacgg aagaaaagtt gcgcggcgca
     6841 gttctccatg cgcttaggaa gacaacctat cactggcaag agctgagcta taccgagggt
     6901 ctgtcccttg tttatatggc agcccggctt gacggaggct ttgctgcagt atcccgcgct
     6961 ttccacgaga ttcgcgctcg caatccagca ttccagcctc agactctgat ggacttcgga
     7021 tctggtacgg gcagtgttac atgggcggca cactccattt ggggtcagag tctgcgagag
     7081 tatatgtgtg tagatcgaag cgcggccatg ctggtcctgg ccgaaaagct gctgaaaggc
     7141 ggctcagaaa gtggagaacc atacattcca ggagtcttct tccgccaatt ccttccagta
     7201 tctcctaaag tacaatttga cgtcgtggtt agtgcgttct cacttagcga gttgccatca
     7261 aaggcggaca ggacggaagt ggtgcaaaca ttgtggagga aaactggtca ttttctcgtc
     7321 cttgtcgaga atggtacaaa agcaggccat agccttctca tggatgcacg agatcttgta
     7381 ttgaagggta aagagaaatc tccactggac cctcggcctg gctttgtttt tgcaccatgt
     7441 ccgcatgaac ttccttgtcc gcagcttacg aatctggcct gttcattttc tcaagcatac
     7501 catcctattc ccttctcatg gaacaagaag cccaaggagg agaaattctc catggtcatt
     7561 cttgcccggg gcagccccga ggaagctcac agatggccca ggataactca gccagtcttg
     7621 aagcggcccc gacatgttca ttgtcatttg tgctgtcccg acggacatat gcagcacgcc
     7681 gtgttgacgg cgcgaagaca cggtcgcgac gccgcagcgt gtgcccgggt atcaagctgg
     7741 ggagatctgc ttccggttct tacgcccagc gcgtttccac cttctactgc tcaggatccg
     7801 tcagagagcg gatctgcggg ttcagccgcc gggagcggtg agttcgatta caaggaccac
     7861 gacggggact acaaagacca tgatatcgat tataaggacg acgacgacaa gtgagaattc
     7921 tcgacctcga gacaaatggc agtattcatc cacaatttta aaagaaaagg ggggattggg
     7981 gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca aactaaagaa
     8041 ttacaaaaac aaattacaaa aattcaaaat tttcgggttt attacaggga cagcagagat
     8101 ccactttggc cgcggctcga gggggttggg gttgcgcctt ttccaaggca gccctgggtt
     8161 tgcgcaggga cgcggctgct ctgggcgtgg ttccgggaaa cgcagcggcg ccgaccctgg
     8221 gtctcgcaca ttcttcacgt ccgttcgcag cgtcacccgg atcttcgccg ctacccttgt
     8281 gggccccccg gcgacgcttc ctgctccgcc cctaagtcgg gaaggttcct tgcggttcgc
     8341 ggcgtgccgg acgtgacaaa cggaagccgc acgtctcact agtctcgtgc agatggacag
     8401 caccgctgag caatggaagc gggtaggcct ttggggcagc ggccaatagc agctttgctc
     8461 cttcgctttc tgggctcaga ggctgggaag gggtgggtcc gggggcgggc tcaggggcgg
     8521 gctcaggggc ggggcgggcg cccgaaggtc ctccggaggc ccggcattct gcacgcttca
     8581 aaagcgcacg tctgccgcgc tgttctcctc ttcctcatct ccgggccttt cgacctgcag
     8641 ccaatatggg atcggccatt gaacaagatg gattgcacgc aggttctccg gccgcttggg
     8701 tggagaggct attcggctat gactgggcac aacagacaat cggctgctct gatgccgccg
     8761 tgttccggct gtcagcgcag gggcgcccgg ttctttttgt caagaccgac ctgtccggtg
     8821 ccctgaatga actgcaggac gaggcagcgc ggctatcgtg gctggccacg acgggcgttc
     8881 cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag ggactggctg ctattgggcg
     8941 aagtgccggg gcagga
//
