LOCUS       sequence-449611-        7932 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Localization: Focal Adhesions, Excitation: 400 / 504, Emission: 515 
            / 517.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7932)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 7932)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mPA-GFP-Vinculin-23
FEATURES             Location/Qualifiers
     source          1..7932
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(11..30)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     358..379
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    3813..3934
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3850..3869)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3904..3923
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(3941..4396)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4078..4099)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4290..4309
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        4423..4527
                     /gene="bla"
                     /label=AmpR promoter
     promoter        4529..4886
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        4529..4720
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(4552..4572)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      4737..4872
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             4920..5714
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             4920..5714
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4974..4993)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5584..5603
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(5902..5921)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    5946..5993
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      6322..6910
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6811..6830
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        7085..7388
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        7389..7592
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     7543..7563
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      7631..7640
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(7637..7639,7640..7642,7643..7932,1..421)
                     /codon_start=1
                     /product="photoactivatable GFP variant (Patterson et al., 
                     2002)"
                     /label=PA-GFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFSYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSHQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(7682..7703)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac
       61 cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg
      121 gcacaagctg gagtacaact acaacagcca caacgtctat atcatggccg acaagcagaa
      181 gaacggcatc aaggccaact tcaagatccg ccacaacatc gaggacggca gcgtgcagct
      241 cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa
      301 ccactacctg agccaccagt ccaagctgag caaagacccc aacgagaagc gcgatcacat
      361 ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcatggacg agctgtacaa
      421 gtccggactc agatctcgag ctcaagcttc gaattcggga aactcgagaa caaagcttga
      481 aaacggccgc atgccagtgt ttcatacgcg cacgatcgag agcatcctgg agccggtggc
      541 acagcagatc tcccacctgg tgataatgca cgaggagggc gaggtggacg gcaaagccat
      601 tcctgacctc accgcgcccg tggccgccgt gcaggcggcc gtcagcaacc tcgtccgggt
      661 tggaaaagag actgttcaaa ccactgagga tcagattttg aagagagata tgccaccagc
      721 atttattaag gttgagaatg cttgcaccaa gcttgtccag gcagctcaga tgcttcagtc
      781 agacccttac tcagtgcctg ctcgagatta tctaattgat gggtcaaggg gcatcctctc
      841 tggaacatca gacctgctcc ttaccttcga tgaggctgag gtccgtaaaa ttattagagt
      901 ttgcaaagga attttggaat atcttacagt ggcagaggtg gtggagacta tggaagattt
      961 ggtcacttac acaaagaatc ttgggccagg aatgactaag atggccaaga tgattgacga
     1021 gagacagcag gagctcactc accaggagca ccgagtgatg ttggtgaact cgatgaacac
     1081 cgtgaaagag ttgctgccag ttctcatttc agctatgaag atttttgtaa caactaaaaa
     1141 ctcaaaaaac caaggcatag aggaagcttt aaaaaatcgc aattttactc tagaaaaaat
     1201 gagtgctgaa attaatgaga taattcgtgt gttacaactc acctcttggg atgaagatgc
     1261 ctgggccagc aaggacactg aagccatgaa gagagcattg gcctccatag actccaaact
     1321 gaaccaggcc aaaggttggc tccgtgaccc tagtgcctcc ccaggggatg ctggtgagca
     1381 ggccatcaga cagatcttag atgaagctgg aaaagttggt gaactctgtg caggcaaaga
     1441 acgcagggag attctgggaa cttgcaaaat gctagggcag atgactgatc aagtggctga
     1501 cctccgtgcc agaggacaag gatcctcacc ggtggccatg cagaaagctc agcaggtatc
     1561 tcagggtctg gatgtgctca cagcaaaagt ggaaaatgca gctcgcaagc tggaagccat
     1621 gaccaactca aagcagagca ttgcaaagaa gatcgatgct gctcagaact ggcttgcaga
     1681 tccaaatggt ggaccggaag gagaagagca gattcgaggt gctttggctg aagctcggaa
     1741 aatagcagaa ttatgtgatg atcctaaaga aagagatgac attctacgtt cccttgggga
     1801 aatatctgct ctgacttcta aattagcaga tctacgaaga caggggaaag gagattctcc
     1861 agaggctcga gccttggcca aacaggtggc cacggccctg cagaacctgc agaccaaaac
     1921 caaccgggct gtggccaaca gcagaccggc caaagcagct gtacaccttg agggcaagat
     1981 tgagcaagca cagcggtgga ttgataatcc cacagtggat gaccgtggag tcggtcaggc
     2041 tgccatccgg gggcttgtgg ccgaagggca tcgtctggct aatgttatga tggggcctta
     2101 tcggcaagat cttctcgcca agtgtgaccg agtggaccag ctgacagccc agctggctga
     2161 cctggctgcc agaggggaag gggagagtcc tcaggcacga gcacttgcat ctcagctcca
     2221 agactcctta aaggatctaa aagctcggat gcaggaggcc atgactcagg aagtgtcaga
     2281 tgttttcagc gataccacaa ctcccatcaa gctgttggca gtggcagcca cggcgcctcc
     2341 tgatgcgcct aacagggaag aggtatttga tgagagggca gctaactttg aaaaccattc
     2401 aggaaagctt ggtgctacgg ccgagaaggc ggctgcggtt ggtactgcta ataaatcaac
     2461 agtggaaggc attcaggcct cagtgaagac ggcccgagaa ctcacacccc aggtggtctc
     2521 ggctgctcgt atcttactta ggaaccctgg aaatcaagct gcttatgaac attttgagac
     2581 catgaagaac cagtggatcg ataatgttga aaaaatgaca gggctggtgg acgaagccat
     2641 tgataccaaa tctctgttgg atgcttcaga agaagcaatt aaaaaagacc tggacaagtg
     2701 caaggtagct atggccaaca ttcagcctca gatgctggtt gctggggcaa ccagtattgc
     2761 tcgtcgggcc aaccggatcc tgctggtggc taagagggag gtggaaaatt ccgaggatcc
     2821 caagttccgt gaggctgtga aagctgcctc tgatgaattg agcaaaacca tctccccgat
     2881 ggtgatggat gcaaaagctg tggctggaaa catttccgac cctggactgc aaaagagctt
     2941 cctggactca ggatatcgga tcctgggagc tgtggccaag gtcagagaag ccttccaacc
     3001 tcaggagcct gacttcccgc cgcctccacc agaccttgaa caactccgac taacagatga
     3061 gcttgctcct cccaaaccac ctctgcctga aggtgaggtc cctccaccta ggcctccacc
     3121 accagaggaa aaggatgaag agttccctga gcagaaggcc ggggaggtga ttaaccagcc
     3181 aatgatgatg gctgccagac agctccatga tgaagctcgc aaatggtcca gcaagggcaa
     3241 tgacatcatt gcagcagcca agcgcatggc tctgctgatg gctgagatgt ctcggctggt
     3301 aagagggggc agtggtacca agcgggcact cattcagtgt gccaaggaca tcgccaaggc
     3361 ctcagatgag gtgactcggt tggccaagga ggttgccaag cagtgcacag ataaacggat
     3421 tagaaccaac ctcttacagg tatgtgagcg aatcccaacc ataagcaccc agctcaaaat
     3481 cctgtccaca gtgaaggcca ccatgctggg ccggaccaac atcagtgatg aggagtctga
     3541 gcaggccaca gagatgctgg ttcacaatgc ccagaacctc atgcagtctg tgaaggagac
     3601 tgtgcgggaa gctgaagctg cttcaatcaa aattcgaaca gatgctggat ttacactgcg
     3661 ctgggttaga aagactccct ggtaccagta ggcggccgcg actctagatc ataatcagcc
     3721 ataccacatt tgtagaggtt ttacttgctt taaaaaacct cccacacctc cccctgaacc
     3781 tgaaacataa aatgaatgca attgttgttg ttaacttgtt tattgcagct tataatggtt
     3841 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta
     3901 gttgtggttt gtccaaactc atcaatgtat cttaaggcgt aaattgtaag cgttaatatt
     3961 ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat tttttaacca ataggccgaa
     4021 atcggcaaaa tcccttataa atcaaaagaa tagaccgaga tagggttgag tgttgttcca
     4081 gtttggaaca agagtccact attaaagaac gtggactcca acgtcaaagg gcgaaaaacc
     4141 gtctatcagg gcgatggccc actacgtgaa ccatcaccct aatcaagttt tttggggtcg
     4201 aggtgccgta aagcactaaa tcggaaccct aaagggagcc cccgatttag agcttgacgg
     4261 ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg
     4321 gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg
     4381 ccgctacagg gcgcgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt
     4441 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg
     4501 cttcaataat attgaaaaag gaagagtcct gaggcggaaa gaaccagctg tggaatgtgt
     4561 gtcagttagg gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc
     4621 atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta
     4681 tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc
     4741 cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta
     4801 tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct
     4861 tttttggagg cctaggcttt tgcaaagatc atcaagagac aggatgagga tcgtttcgca
     4921 tgattgaaca agatggattg cacgcaggtt ctccggccgc ttgggtggag aggctattcg
     4981 gctatgactg ggcacaacag acaatcggct gctctgatgc cgccgtgttc cggctgtcag
     5041 cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc
     5101 aagacgaggc agcgcggcta tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc
     5161 tcgacgttgt cactgaagcg ggaagggact ggctgctatt gggcgaagtg ccggggcagg
     5221 atctcctgtc atctcacctt gctcctgccg agaaagtatc catcatggct gatgcaatgc
     5281 ggcggctgca tacgcttgat ccggctacct gcccattcga ccaccaagcg aaacatcgca
     5341 tcgagcgagc acgtactcgg atggaagccg gtcttgtcga tcaggatgat ctggacgaag
     5401 agcatcaggg gctcgcgcca gccgaactgt tcgccaggct caaggcgagc atgcccgacg
     5461 gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg gtggaaaatg
     5521 gccgcttttc tggattcatc gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca
     5581 tagcgttggc tacccgtgat attgctgaag agcttggcgg cgaatgggct gaccgcttcc
     5641 tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat cgccttctat cgccttcttg
     5701 acgagttctt ctgagcggga ctctggggtt cgaaatgacc gaccaagcga cgcccaacct
     5761 gccatcacga gatttcgatt ccaccgccgc cttctatgaa aggttgggct tcggaatcgt
     5821 tttccgggac gccggctgga tgatcctcca gcgcggggat ctcatgctgg agttcttcgc
     5881 ccaccctagg gggaggctaa ctgaaacacg gaaggagaca ataccggaag gaacccgcgc
     5941 tatgacggca ataaaaagac agaataaaac gcacggtgtt gggtcgtttg ttcataaacg
     6001 cggggttcgg tcccagggct ggcactctgt cgatacccca ccgagacccc attggggcca
     6061 atacgcccgc gtttcttcct tttccccacc ccacccccca agttcgggtg aaggcccagg
     6121 gctcgcagcc aacgtcgggg cggcaggccc tgccatagcc tcaggttact catatatact
     6181 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
     6241 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
     6301 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     6361 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     6421 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta
     6481 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     6541 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     6601 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     6661 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
     6721 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     6781 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     6841 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     6901 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
     6961 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgccat
     7021 gcattagtta ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt
     7081 tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc
     7141 cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac
     7201 gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata
     7261 tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc
     7321 agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta
     7381 ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac
     7441 ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc
     7501 aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc
     7561 gtgtacggtg ggaggtctat ataagcagag ctggtttagt gaaccgtcag atccgctagc
     7621 gctaccggtc gccaccatgg tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat
     7681 cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga
     7741 gggcgatgcc acctacggca agctgaccct gaagttcatc tgcaccaccg gcaagctgcc
     7801 cgtgccctgg cccaccctcg tgaccacctt cagctacggc gtgcagtgct tcagccgcta
     7861 ccccgaccac atgaagcagc acgacttctt caagtccgcc atgcccgaag gctacgtcca
     7921 ggagcgcacc at
//
