LOCUS       sequence-449617-       10488 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CasRx guide RNA cloning backbone (lenti).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10488)
  AUTHORS   Wei J, Lotfy P, Faizi K, Baungaard S, Gibson E, Wang E, Slabodkin H,
            Kinnaman E, Chandrasekaran S, Kitano H, Durrant MG, Duffy CV, Pawluk
            A, Hsu PD, Konermann S
  TITLE     Deep learning and CRISPR-Cas13d ortholog discovery for optimized RNA
            targeting.
  JOURNAL   Cell Syst. 2023 Dec 20;14(12):1087-1102.e13. doi: 
            10.1016/j.cels.2023.11.006. Epub 2023 Dec 12.
  PUBMED    38091991
REFERENCE   2  (bases 1 to 10488)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cels.2023.11.006"; journalName: "Cell Syst"; date: 
            "2023-12-20- 20"; volume: "14"; issue: "12"; pages: "1087-1102.e13"
COMMENT     Sequence Label: pLV-EF1a-mTagBFP2-2A-Puro_hU6-RfxDR36-BsmBI
FEATURES             Location/Qualifiers
     source          1..10488
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    877..898
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(913..918,919..936,937..943)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    951..967
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     956..978
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     975..991
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     975..991
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     polyA_signal    1040..1161
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1051..1070)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     1105..1124
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(1291..1665)
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     promoter        complement(1684..1731)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     promoter        complement(1779..2108)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      1793..1928
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(1847..1866)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     2093..2113
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      complement(2122..2550)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2232..2253)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2444..2463
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     polyA_signal    2596..2820
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     2809..2826
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     LTR             2852..3085
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     misc_feature    3157..3745
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             3263..3274
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     3672..3692
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3761..4357)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="TEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIERV
                     TELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLAA
                     QQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETSA
                     PRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3844..3864)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             complement(4358..4414)
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             complement(4424..5134)
                     /codon_start=1
                     /product="enhanced monomeric blue fluorescent protein 
                     (Subach et al., 2011)"
                     /label=mTagBFP2
                     /note="mammalian codon-optimized"
                     /translation="MVSRGEELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRND
                     MALKLVGGSHLIANAKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAV
                     ARYCDLPSKLGHKLN"
     regulatory      5131..5140
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(5147..6325)
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          5156..6094
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     complement(5179..5199)
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     misc_RNA        complement(6432..6467)
                     /label=RfxCas13d DR36
                     /note="36-base direct repeat for an RfxCas13d pre-gRNA 
                     (Konermann et al., 2018)"
     promoter        complement(6469..6717)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     complement(6527..6546)
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(6697..6717)
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     misc_feature    6768..6885
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(7183..7227)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    7412..7645
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    8138..8263
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             8310..8490
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(8508..8706)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(8533..8553)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        8708..9087
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     9259..9278
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        9353..9457
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9458..9526,9527..10318)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9676..9695)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
      841 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
      901 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
      961 ataacaattt cacacaggaa acagctatga ccatgattac gccaagctct agctagaggt
     1021 cgacggtata cagacatgat aagatacatt gatgagtttg gacaaaccac aactagaatg
     1081 cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt
     1141 ataagctgca ataaacaagt tggggtgggc gaagaactcc agcatgagat ccccgcgctg
     1201 gaggatcatc cagccggcgt cccggaaaac gattccgaag cccaaccttt catagaaggc
     1261 ggcggtggaa tcgaaatctc gtagcacgtg tcagtcctgc tcctcggcca cgaagtgcac
     1321 gcagttgccg gccgggtcgc gcagggcgaa ctcccgcccc cacggctgct cgccgatctc
     1381 ggtcatggcc ggcccggagg cgtcccggaa gttcgtggac acgacctccg accactcggc
     1441 gtacagctcg tccaggccgc gcacccacac ccaggccagg gtgttgtccg gcaccacctg
     1501 gtcctggacc gcgctgatga acagggtcac gtcgtcccgg accacaccgg cgaagtcgtc
     1561 ctccacgaag tcccgggaga acccgagccg gtcggtccag aactcgaccg ctccggcgac
     1621 gtcgcgcgcg gtgagcaccg gaacggcact ggtcaacttg gccatggttt agttcctcac
     1681 cttgtcgtat tatactatgc cgatatacta tgccgatgat taattgtcaa cacgtgctga
     1741 tcagatccga aaatggatat acaagctccc gggagctttt tgcaaaagcc taggcctcca
     1801 aaaaagcctc ctcactactt ctggaatagc tcagaggcag aggcggcctc ggcctctgca
     1861 taaataaaaa aaattagtca gccatggggc ggagaatggg cggaactggg cggagttagg
     1921 ggcgggatgg gcggagttag gggcgggact atggttgctg actaattgag atgcatgctt
     1981 tgcatacttc tgcctgctgg ggagcctggg gactttccac acctggttgc tgactaattg
     2041 agatgcatgc tttgcatact tctgcctgct ggggagcctg gggactttcc acaccctaac
     2101 tgacacacat tccacagaat taattcgcgt taaatttttg ttaaatcagc tcatttttta
     2161 accaataggc cgaaatcggc aaaatccctt ataaatcaaa agaatagacc gagatagggt
     2221 tgagtgttgt tccagtttgg aacaagagtc cactattaaa gaacgtggac tccaacgtca
     2281 aagggcgaaa aaccgtctat cagggcgatg gcccactacg tgaaccatca ccctaatcaa
     2341 gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg agcccccgat
     2401 ttagagcttg acggggaaag ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag
     2461 gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc accacacccg
     2521 ccgcgcttaa tgcgccgcta cagggcgcgt ggggataccc cctagagccc cagctggttc
     2581 tttccgcctc agaagccata gagcccaccg catccccagc atgcctgcta ttgtcttccc
     2641 aatcctcccc cttgctgtcc tgccccaccc caccccccag aatagaatga cacctactca
     2701 gacaatgcga tgcaatttcc tcattttatt aggaaaggac agtgggagtg gcaccttcca
     2761 gggtcaagga aggcacgggg gaggggcaaa caacagatgg ctggcaacta gaaggcacag
     2821 tcgaggctga tcagcgggtt taaacgggcc ctgctagaga ttttccacac tgactaaaag
     2881 ggtctgaggg atctctagtt accagagtca cacaacagac gggcacacac tacttgaagc
     2941 actcaaggca agctttattg aggcttaagc agtgggttcc ctagttagcc agagagctcc
     3001 caggctcaga tctggtctaa ccagagagac ccagtacaag caaaaagcag atcttgtctt
     3061 cgttgggagt gaattagccc ttccagtccc cccttttctt ttaaaaagtg gctaagatct
     3121 acagctgcct tgtaagtcat tggtcttaaa gtcgacgcgg ggaggcggcc caaagggaga
     3181 tccgactcgt ctgagggcga aggcgaagac gcggaagagg ccgcagagcc ggcagcaggc
     3241 cgcgggaagg aaggtccgct ggattgaggg ccgaagggac gtagcagaag gacgtcccgc
     3301 gcagaatcca ggtggcaaca caggcgagca gccaaggaaa ggacgatgat ttccccgaca
     3361 acaccacgga attgtcagtg cccaacagcc gagcccctgt ccagcagcgg gcaaggcagg
     3421 cggcgatgag ttccgccgtg gcaataggga gggggaaagc gaaagtcccg gaaaggagct
     3481 gacaggtggt ggcaatgccc caaccagtgg gggttgcgtc agcaaacaca gtgcacacca
     3541 cgccacgttg cctgacaacg ggccacaact cctcataaag agacagcaac caggatttat
     3601 acaaggagga gaaaatgaaa gccatacggg aagcaatagc atgatacaaa ggcattaaag
     3661 cagcgtatcc acatagcgta aaaggagcaa catagttaag aataccagtc aatctttcac
     3721 aaattttgta atccagaggt tgattgtcga cttaacgcgt tcaggcaccg ggcttgcggg
     3781 tcatgcacca ggtgcgcggt ccttcgggca cctcgacgtc ggcggtgacg gtgaagccga
     3841 gccgctcgta gaaggggagg ttgcggggcg cggaggtctc caggaaggcg ggcaccccgg
     3901 cgcgctcggc cgcctccact ccggggagca cgacggcgct gcccagaccc ttgccctggt
     3961 ggtcgggcga gactccgacg gtggccagga accacgcggg ctccttgggc cggtgcggcg
     4021 ccaggaggcc ttccatctgt tgctgcgcgg ccagccggga accgctcaac tcggccatgc
     4081 gcgggccgat ctcggcgaac accgcccccg cttcgacgct ctccggcgtg gtccagaccg
     4141 ccaccgcggc gccgtcgtcc gcgacccaca ccttgccgat gtcgagcccg acgcgcgtga
     4201 ggaagagttc ttgcagctcg gtgacccgct cgatgtggcg gtccggatcg acggtgtggc
     4261 gcgtggcggg gtagtcggcg aacgcggcgg cgagggtgcg tacggccctg gggacgtcgt
     4321 cgcgggtggc gaggcgcacc gtgggcttgt actcggtcgg tccaggattc tcttcgacat
     4381 ctccggcttg tttcagcaga gagaagtttg ttgcgccgga tccattaagc ttgtgcccca
     4441 gtttgctagg gaggtcgcag tatctggcca ctgccacctc gtgctgctcg acgtaggtct
     4501 cgttgttggc ctccttgatt ctttccagtc tgtagtccac atagtagacg ccaggcatct
     4561 tgaggttctt agcgggtttc ttggatctat atgtggtctt ggcgtttgcg atcagatggc
     4621 tcccgcccac gagcttcagg gccatgtcgt ttctgccttc caggccgccg tcagcggggt
     4681 acagcgtttc ggtgaaggcc tcccagccga gtgttttctt ctgcatcaca gggccgttgg
     4741 atgtgaagtt cacccctctg atcttgacgt tgtagatgag gcagccgtcc tggaggctgg
     4801 tgtcctgggt agcggtcagc acgcccccgt cttcgtatgt ggtgactctc tcccatgtga
     4861 agccctcagg gaaggactgc ttgaagaagt cggggatgcc ctgggtgtgg ttgatgaagg
     4921 tcttgctgcc gtagaggaag ctagtagcca ggatgtcgaa ggcgaagggg agagggccgc
     4981 cctcgaccac cttgattctc atggtctggg tgccctcgta gggcttgcct tcgccctcgg
     5041 atgtgcactt gaagtgatgg ttgtccacgg tgccctccat gtacagcttc atgtgcatgt
     5101 tctccttaat cagctcttcg cccctagaca ccatggtggc cgtacgtcac gacacctgaa
     5161 atggaagaaa aaaactttga accactgtct gaggcttgag aatgaaccaa gatccaaact
     5221 caaaaagggc aaattccaag gagaattaca tcaagtgcca agctggccta acttcagtct
     5281 ccacccactc agtgtgggga aactccatcg cataaaaccc ctccccccaa cctaaagacg
     5341 acgtactcca aaagctcgag aactaatcga ggtgcctgga cggcgcccgg tactccgtgg
     5401 agtcacatga agcgacggct gaggacggaa aggccctttt cctttgtgtg ggtgactcac
     5461 ccgcccgctc tcccgagcgc cgcgtcctcc attttgagct ccctgcagca gggccgggaa
     5521 gcggccatct ttccgctcac gcaactggtg ccgaccgggc cagccttgcc gcccagggcg
     5581 gggcgataca cggcggcgcg aggccaggca ccagagcagg ccggccagct tgagactacc
     5641 cccgtccgat tctcggtggc cgcgctcgca ggccccgcct cgccgaacat gtgcgctggg
     5701 acgcacgggc cccgtcgccg cccgcggccc caaaaaccga aataccagtg tgcagatctt
     5761 ggcccgcatt tacaagacta tcttgccaga aaaaaagcgt cgcagcaggt catcaaaaat
     5821 tttaaatggc tagagactta tcgaaagcag cgagacaggc gcgaaggtgc caccagattc
     5881 gcacgcggcg gccccagcgc ccaggccagg cctcaactca agcacgaggc gaaggggctc
     5941 cttaagcgca aggcctcgaa ctctcccacc cacttccaac ccgaagctcg ggatcaagaa
     6001 tcacgtactg cagccaggtg gaagtaattc aaggcacgca agggccataa cccgtaaaga
     6061 ggccaggccc gcgggaacca cacacggcac ttacctgtgt tctggcggca aacccgttgc
     6121 gaaaaagaac gttcacggcg actactgcac ttatatacgg ttctccccca ccctcgggaa
     6181 aaaggcggag ccagtacacg acatcacttt cccagtttac cccgcgccac cttctctagg
     6241 caccggttca attgccgacc cctcccccca acttctcggg gactgtgggc gatgtgcgct
     6301 ctgcccactg acgggcaccg gagccaattc ccactccttt caagacctag aaggtccatt
     6361 agctgcaaag attcctctct gtttaaaact ttatccatct ttgcagaatt caaaaaagga
     6421 gacgcgtctc tgtttcaaac cccgaccagt tggtaggggt ttacttgcgg tgtttcgtcc
     6481 tttccacaag atatataaag ccaagaaatc gaaatacttt caagttacgg taagcatatg
     6541 atagtccatt ttaaaacata attttaaaac tgcaaactac ccaagaaatt attactttct
     6601 acgtcacgta ttttgtacta atatctttgt gtttacagtc aaattaattc taattatctc
     6661 tctaacagcc ttgtatcgta tatgcaaata tgaaggaatc atgggaaata ggccctcggt
     6721 accttaatta accaaactgg atctctgctg tccctgtaat aaacccgaaa attttgaatt
     6781 tttgtaattt gtttttgtaa ttctttagtt tgtatgtctg ttgctattat gtctactatt
     6841 ctttcccctg cactgtaccc cccaatcccc ccttttcttt taaaattgtg gatgaatact
     6901 gccatttgtc tgcagaattg gcgcacgcag tgccgatccg ttcactaatc gaatggatct
     6961 gtctctgtct ctctctccac cttcttcttc tattccttcg ggcctgtcgg gtcccctcgg
     7021 ggttgggagg tgggtctgaa acgataatgg tgaatatccc tgcctaactc tattcactat
     7081 agaaagtaca gcaaaaacta ttcttaaacc taccaagcct cctactatca ttatgaataa
     7141 ttttatatac cacagccaat ttgttatgtt aaaccaattc cacaaacttg cccatttatc
     7201 taattccaat aattcttgtt cattcttttc ttgctggttt tgcgattctt caattaagga
     7261 gtgtattaag cttgtgtaat tgttaatttc tctgtcccac tccatccagg tcgtgtgatt
     7321 ccaaatctgt tccagagatt tattactcca actagcattc caaggcacag cagtggtgca
     7381 aatgagtttt ccagagcaac cccaaatccc caggagctgt tgatccttta ggtatctttc
     7441 cacagccagg attcttgcct ggagctgctt gatgccccag actgtgagtt gcaacagatg
     7501 ctgttgcgcc tcaatagccc tcagcaaatt gttctgctgc tgcactatac cagacaataa
     7561 ttgtctggcc tgtaccgtca gcgtcattga cgctgcgccc atagtgcttc ctgctgctcc
     7621 caagaaccca aggaacaaag ctcctattcc cactgctctt ttttctctct gcaccactct
     7681 tctctttgcc ttggtgggtg ctactcctaa tggttcaatt tttactactt tatatttata
     7741 taattcactt ctccaattgt ccctcatatc tcctcctcca ggtctgaaga tcagcggccg
     7801 cttgctgtgc ggtggtctta cttttgtttt gctcttcctc tatcttgtct aaagcttcct
     7861 tggtgtcttt tatctctatc ctttgatgca cacaatagag ggttgctact gtattatata
     7921 atgatctaag ttcttctgat cctgtctgaa gggatggttg tagctgtccc agtatttgtc
     7981 tacagccttc tgatgtttct aacaggccag gattaactgc gaatcgttct agctccctgc
     8041 ttgcccatac tatatgtttt aatttatatt ttttctttcc ccctggcctt aaccgaattt
     8101 tttcccatcg cgatctaatt ctcccccgct taatactgac gctctcgcac ccatctctct
     8161 ccttctagcc tccgctagtc aaaatttttg gcgtactcac cagtcgccgc ccctcgcctc
     8221 ttgccgtgcg cgcttcagca agccgagtcc tgcgtcgaga gagctcctct ggtttccctt
     8281 tcgctttcaa gtccctgttc gggcgccact gctagagatt ttccacactg actaaaaggg
     8341 tctgagggat ctctagttac cagagtcaca caacagacgg gcacacacta cttgaagcac
     8401 tcaaggcaag ctttattgag gcttaagcag tgggttccct agttagccag agagctccca
     8461 ggctcagatc tggtctaacc agagagaccc agtacaggca aaacgcgctg cttatataga
     8521 cctcccaccg tacacgccta ccgcccattt gcgtcaatgg ggcggagttg ttacgacatt
     8581 ttggaaagtc ccgttgattt tggtgccaaa acaaactccc attgacgtca atggggtgga
     8641 gacttggaaa tccccgtgag tcaaaccgct atccacgccc attgatgtac tgccaaaacc
     8701 gcatcaccat ggtaatagcg atgactaata cgtagatgta ctgccaagta ggaaagtccc
     8761 ataaggtcat gtactgggca taatgccagg cgggccattt accgtcattg acgtcaatag
     8821 ggggcgtact tggcatatga tacacttgat gtactgccaa gtgggcagtt taccgtaaat
     8881 actccaccca ttgacgtcaa tggaaagtcc ctattggcgt tactatggga acatacgtca
     8941 ttattgacgt caatgggcgg gggtcgttgg gcggtcagcc aggcgggcca tttaccgtaa
     9001 gttatgtaac gcggaactcc atatatgggc tatgaactaa tgaccccgta attgattact
     9061 attaataact agtcaataat caatgtcaac gcgtatatct ggcccgtaca tcgcgaagca
     9121 gcgcaaaacg cctaacccta agcagattct tcatgcaatt gtcggtcaag ccttgccttg
     9181 ttgtagctta aattttgctc gcgcactact cagcgacctc caacacacaa gcagggagca
     9241 gatactggct taactatgcg gcatcagagc agattgtact gagagtgcac cataggggat
     9301 cgggagatct cccgatccgt cgacgtcagg tggcactttt cggggaaatg tgcgcggaac
     9361 ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc
     9421 ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt
     9481 cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct
     9541 ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga
     9601 tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag
     9661 cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca
     9721 actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga
     9781 aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag
     9841 tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc
     9901 ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa
     9961 tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt
    10021 gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg
    10081 gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt
    10141 tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg
    10201 gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat
    10261 ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact
    10321 gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa
    10381 aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt
    10441 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttc
//
