LOCUS       sequence-449634-       18991 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  codon optimized Halo tagged VPS13B for expression in mammalian 
            cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 18991)
  AUTHORS   Ugur B, Schueder F, Shin J, Hanna MG, Wu Y, Leonzino M, Su M, McAdow
            AR, Wilson C, Postlethwait J, Solnica-Krezel L, Bewersdorf J, De 
            Camilli P
  TITLE     VPS13B is localized at the cis-trans Golgi complex interface and is 
            a functional partner of FAM177A1.
  JOURNAL   bioRxiv [Preprint]. 2023 Dec 18:2023.12.18.572081. doi: 
            10.1101/2023.12.18.572081.
  PUBMED    38187698
REFERENCE   2  (bases 1 to 18991)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            [Preprint]. 2023 Dec 18:2023.12.18.572081. doi: 
            10.1101/2023.12.18.572081."
COMMENT     Sequence Label: VPS13B^Halo
FEATURES             Location/Qualifiers
     source          1..18991
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    8905..9493
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8958..8978)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9376..9387)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(9515..9534)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    9559..9607
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      9809..10237
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9896..9915)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10106..10127
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(10246..10266)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        10251..10580
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      10431..10566
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     10493..10512
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             10647..11441
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(10701..10720)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     11311..11330
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    11617..11738
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(11654..11673)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     11708..11727
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(11787..11803)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11787..11803)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(11800..11822)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    11811..11827
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(11835..11841,11842..11859,11860..11865))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11880..11901
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(12018..12035)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(12189..12777)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(12269..12288)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(12948..13739,13740..13808))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     13571..13590
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(13809..13913)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(13988..14007)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        14179..14558
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        14559..14762
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     14713..14733
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     14759..14783
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             join(18791..18991,1..690)
                     /codon_start=1
                     /product="modified bacterial dehalogenase that forms 
                     covalent bonds with chloroalkane derivatives"
                     /label=HaloTag(R)
                     /translation="MAEIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPTSS
                     YVWRNIIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVI
                     HDWGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLII
                     DQNVFIEGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVALV
                     EEYMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPGLNLLQEDNPDL
                     IGSEIARWLSTLEISG"
ORIGIN
        1 ggtatgggca aatccgacaa accagacctg ggttatttct tcgacgacca cgtccgcttc
       61 atggatgcct tcatcgaagc cctgggtctg gaagaggtcg tcctggtcat tcacgactgg
      121 ggctccgctc tgggtttcca ctgggccaag cgcaatccag agcgcgtcaa aggtattgca
      181 tttatggagt tcatccgccc tatcccgacc tgggacgaat ggccagaatt tgcccgcgag
      241 accttccagg ccttccgcac caccgacgtc ggccgcaagc tgatcatcga tcagaacgtt
      301 tttatcgagg gtacgctgcc gatgggtgtc gtccgcccgc tgactgaagt cgagatggac
      361 cattaccgcg agccgttcct gaatcctgtt gaccgcgagc cactgtggcg cttcccaaac
      421 gagctgccaa tcgccggtga gccagcgaac atcgtcgcgc tggtcgaaga atacatggac
      481 tggctgcacc agtcccctgt cccgaagctg ctgttctggg gcaccccagg cgttctgatc
      541 ccaccggccg aagccgctcg cctggccaaa agcctgccta actgcaaggc tgtggacatc
      601 ggcccgggtc tgaatctgct gcaagaagac aacccggacc tgatcggcag cgagatcgcg
      661 cgctggctgt cgacgctcga gatttccggc ggcggcggat ccggcggatt cagtgatagc
      721 gtgacccttg aacagaccac ttccaatatc ggtggtacca gtggccgggt gtctctgtgg
      781 atgcagtggg tcttgcctaa aatcacaatt aagctgttcg cccctgaccc cgagaataag
      841 ggtaccgaag tgtgcatggt gtctgaactg gaggacctgt ccgccagcat tgacgtgcag
      901 gacgtgtaca ccaaggtcaa gtgcaagatc gagtccttta acatagacca ctaccgctct
      961 tctctggggg aggaatgctg gtccctgggc cagtgtggcg gcgtgtttct tagctgcacc
     1021 gataagctca acagacgcac cctccttgtg agacctatct ccaagcaaga ccccttcagt
     1081 aactgctccg gattttttcc tagcaccacc acaaagctgc tggacggaac ccatcagcaa
     1141 cacggcttct tatctctgac ctacaccaag gctgtcacca agaacgtgag acataagctg
     1201 accagccgga atgagagaag atctttccac aagctatccg aagggctcat ggacgggtcc
     1261 ccccacttcc tgcacgaaat actgctctcc gcccaagcct tcgacatagt tctgtacttt
     1321 cccctactga acgccatcgc cagcatcttc caggccaagc tgcctaagac acaaaaggaa
     1381 aagcggaaga gccccggaca gccaatgcgg actcacacac tcaccagcag aaacctgccc
     1441 ctcatctacg tgaatacttc ggtgatcaga atctttatcc ccaagacaga agagatgcag
     1501 ccgaccgtgg aagccaacca ggccgccaaa gaagatacag tggttctaaa aatcggctct
     1561 gttgcaatgg ccccgcaggc cgacaaccct ctgggaagaa gcgtgctgag aaaggatatc
     1621 tatcagcggg ccctgaacct gggcatactg agagaccctg gaagcgaaat cgaagaccga
     1681 cagtaccaga tcgatctgca gagcatcaac atcggcacgg cccaatggca ccagctgaag
     1741 cctgagaagg agtctgtgtc cgggggcgtc gttacagaga ccgaaagaaa ctcccaaaac
     1801 cccgccctgg agtggaacat ggcgagcagc ataaggcggc accaggagag aagagccatc
     1861 ctgacccctg tgctcacaga tttctcagta agaatcaccg gcgctcccgc agtgattttt
     1921 actaaagtgg tatctcctga aaacctccat acagaagaaa tcctcgtctg cggccacagt
     1981 ctcgaggtga atatcactac caacctcgac ttcttcctgt ctgtcgcaca ggtccagctg
     2041 ctgcaccagc tgattgtggc caatatgaca ggcctggaac ccagcaacaa ggctgccgaa
     2101 atttccaagc aggaacagaa gaaggtcgat atatttgacg gaggcatggc cgagacaagc
     2161 agccggtaca gtggcgcaca ggacagcggc attggatctg atagtgtcaa gatcagaata
     2221 gtgcagatcg agcagcacag cggcgcctct cagcatagga ttgcaagacc gtccagacag
     2281 tcaagcatcg tgaaaaacct gaacttcatc ccctttgaca ttttcatcac cgcctcaaga
     2341 atcagcctga tgacctatag ctgcatggct ttgagcaagt caaagtccca agaacagaag
     2401 aacaacgaaa agacagacaa atctagcctg aacctacccg aagtagatag cgatgtggcc
     2461 aagcctaatc aggcctgcat cagcacagtc acagctgagg atctactgag atcctccatc
     2521 tcttttccta gtggtaaaaa aatcggggtc ttatcattgg aatcgctgca cgcctccact
     2581 agaagctctg cacggcaggc tctaggcatc acaatcgtga gacagcctgg cagaagaggt
     2641 acaggcgacc tgcagctcga gcccttcctt tatttcatcg tgtcccagcc tagtctcctg
     2701 ctgtcgtgcc atcacagaaa gcagagagtg gaggtttcca tcttcgacgc cgttctaaag
     2761 ggcgtcgcct ctgactacaa gtgtattgac ccgggaaaaa ccctgcccga ggcacttgat
     2821 tactgtaccg tgtggctgca aaccgtgccc ggcgagattg actccaagtc cggaatccct
     2881 cccagtttca ttacactgca aatcaaagat ttcctcaacg gccctgccga cgtgaacctg
     2941 gacatcagta agcctctcaa agcaaacctg agtttcacaa agctggatca gatcaacctg
     3001 tttctgaaga aaataaagaa tgcccactca ctcgctcact ctgaggaaac ctctgccatg
     3061 tctaatacca tggtgaacaa agacgatctg cccgtgtcaa agtactatag aggcaaactt
     3121 agtaaaccta aaattcacgg cgatggagta caaaagatct cggcccagga aaatatgtgg
     3181 cgagctgtct cctgcttcca gaaaatttcc gttcagacga cccagatcgt catcagtatg
     3241 gaaaccgttc ctcacacctc gaagccttgt ctgctcgctt ctctgtccaa tctgaacggc
     3301 tccctgtcag tgaaggccac acagaaagtg cccggaatca ttctgggctc gtctttcctg
     3361 ctgtcaatca acgacttcct gctgaagacc tcccttaagg aacggagcag aatcctgatt
     3421 gggccatgct gcgccacagc gaacctggaa gctaaatggt gcaagcactc tggaaatcct
     3481 ggtcctgagc aatctatccc aaagatcagt atcgacctta ggggcggcct gctgcaagtg
     3541 ttctggggac aggagcacct caattgcctg gtgctgctgc acgagttact gaatgggtac
     3601 ctaaatgagg agggcaactt cgaggtacag gtgagtgagc ctgtgccaca gatgagcagc
     3661 ccggttgaga agaatcagac attcaagagc gagcagagct cagatgatct cagaactggc
     3721 ctgttccagt acgttcagga tgccgagagc ctgaagctgc ctggagtgta cgaagttcta
     3781 ttctacaacg agactgaaga ctgccccggc atgatgctct ggcggtaccc agaacctaga
     3841 gtgctgaccc tggtgcgcat cacaccagtg ccattcaaca ccaccgaaga tcctgatatc
     3901 agtaccgccg acctggggga tgtgctgcag gtcccttgca gcctcgaata ctgggatgag
     3961 ctgcagaagg tattcgtggc ctttagagag ttcaatctgt ctgaatcaaa ggtgtgcgag
     4021 ctgcaattac ctgacatcaa cctcgttaac gaccagaaga aactggtttc tagcgacctg
     4081 tggcggatcg ttcttaacag ctcacaaaac ggcgccgatg atcagtccag tgcctcggaa
     4141 tctggctccc agtctacctg cgaccctctc gtcactccga ccgcgctcgc cgcctgcaca
     4201 cgggtggata gctgctttac cccatggttc gtgccctctc tgtgcgtgag cttccagttc
     4261 gcccacttgg aatttcactt atgccaccat ctggatcaac tgggcaccgc cgccccccaa
     4321 taccttcagc ccttcgtgag cgaccggaac atgcctagcg agttggagta catgattgtg
     4381 tctttcagag agccccatat gtacctgaga cagtggaaca acgggtctgt atgccaggag
     4441 atccaattcc tggcccaggc ggactgcaag ctcctggagt gcagaaacgt gacaatgcag
     4501 agtgtggtga aacctttttc tatcttcggt cagatggccg tgtcctccga cgtggtggag
     4561 aaactgttag attgtaccgt tatcgtagac agcgtctttg taaacctggg ccagcatgtg
     4621 gttcattcgt tgaacactgc gatccaggct tggcagcaaa ataagtgccc tgaagtggag
     4681 gaactggtgt tctcgcattt cgtgatctgt aacgacacac aggagaccct gagattcggc
     4741 caggtagaca ccgacgaaaa tattttgctt gcctctctac acagtcacca atactcctgg
     4801 agatcccaca agtcccctca actcctgcac atctgcatcg agggatgggg caactggcgg
     4861 tggagcgagc cattctccgt ggaccacgcc ggcactttta ttcggaccat acaatatcgg
     4921 gggagaaccg ctagcctcat catcaaggtt cagcagctga acggggtcca gaagcaaatc
     4981 attatctgtg gccgtcaaat catctgctca tacctgtctc agtccatcga gctgaaggtg
     5041 gtgcagcact acattggcca agatggccag gccgtagttc gggaacactt cgattgcctg
     5101 accgccaagc agaagctgcc ttcctacatc ctggagaaca atgagcttac agagctgtgc
     5161 gtgaaggcga agggggacga ggactggtcc agagatgttt gtttagaaag caaagcaccg
     5221 gaatactcta tcgtaatcca ggtgccatct agcaactcgt ctatcatcta cgtgtggtgc
     5281 accgtgctga cactggagcc caacagccaa gtgcagcaaa gaatgatcgt gttttcccca
     5341 ctgttcatca tgagatccca cctgccagac ccaatcatca tccacctaga aaagagatct
     5401 ctcggcctgt ccgagactca gatcatacct ggtaagggcc aagaaaaacc actgcagaat
     5461 atcgagcctg atctggtgca ccacctgacc ttccaggcta gagaagagta cgatccctcc
     5521 gactgcgccg tcccaatctc tacctctctt atcaagcaga tcgcaacaaa agtgcacccc
     5581 ggcggcaccg tcaaccagat cctggacgag ttctacggcc ctgaaaagag cctgcagccc
     5641 atctggcctt acaacaaaaa ggattccgac agaaacgagc agctgtcaca gtgggacagt
     5701 ccaatgcggg tgaagttatc aatctggaaa ccctacgtgc ggacgttact gatagagctg
     5761 ttaccttggg cgttgctgat aaacgagtca aagtgggacc tgtggctgtt tgagggcgag
     5821 aaaatcgtgc ttcaagtacc agccggcaag atcattatcc ctccaaactt ccaggaggcc
     5881 tttcagatcg gcatctactg ggccaacacc aacaccgtcc ataagtctgt ggccattaag
     5941 ttagtgcata acttgacctc tcctaaatgg aaggacggcg gcaacggaga ggttgtgacc
     6001 ctggacgaag aggccttcgt ggacaccgag atcagacttg gtgcattccc tggccaccag
     6061 aagctgtgtc agttctgcat cagctccatg gtccagcagg gcatccagat cattcagatt
     6121 gaagacaaga caaccattat caacaacaca ccctaccaga tcttctacaa acctcagtta
     6181 tccgtgtgta accctcacag cggcaaggaa tatttccgag tgcctgattc tgccacgttc
     6241 tcaatctgcc ctggcggaga gcagcctgct atgaagtcct cgtccctgcc ttgctgggat
     6301 ctgatgccag acatctccca gagcgtactc gacgccagtc tcctacagaa acagatcatg
     6361 ctgggtttca gtccagctcc aggcgccgac agcagccaat gttggagcct tcctgctata
     6421 gttcggcccg aattccctcg gcagagcgtg gccgtgcctt taggaaattt cagagaaaac
     6481 ggcttttgca caagagccat tgtgctaaca taccaagagc acctgggagt gacttacctg
     6541 acgctgagcg aagatccatc tcccagagtc atcatccaca acagatgccc cgttaagatg
     6601 ctgattaagg agaacattaa ggacatcccg aagttcgaag tgtactgcaa gaaaatcccg
     6661 tccgagtgca gcatacacca tgaactctac caccagattt ccagctaccc cgattgcaag
     6721 acgaaggacc tgctgcccag cttgctgctg agagtggaac ctctggacga ggtgactaca
     6781 gagtggtctg acgccatcga catcaacagc cagggcacac aagtagtttt cttgacagga
     6841 tttggctacg tctacgtcga tgtcgttcac cagtgtggca ctgtgttcat tactgtagcc
     6901 cctgaaggga aggctggccc cattctcacc aacaccaaca gagccccgga aaaaattgtg
     6961 acctttaaga tgttcatcac acaactgtca ttggctgtgt ttgatgatct gacccaccac
     7021 aaagccagcg cggagctcct gcgactcacc ttagataaca tctttctgtg cgtcgcccca
     7081 ggcgctggcc cactgcctgg cgaggaaccc gtcgccgccc tgttcgagct gtattgcgtt
     7141 gagatttgct gcggcgacct acaactggac aaccagctgt acaacaaaag caatttccac
     7201 tttgccgtcc tggtttgcca gggcgaaaag gctgaaccaa tccagtgtag caagatgcag
     7261 agcctgctta tcagcaacaa ggaattggag gagtacaagg aaaagtgttt catcaagctg
     7321 tgcattaccc tgaacgaagg caaatccatc ctctgcgata tcaatgaatt tagcttcgaa
     7381 ctcaaaccag ccagactgta cgttgaggat acattcgtgt actacatcaa gactctcttc
     7441 gacacatacc tgcctaacag ccgtctagcc ggacacagta cccacctgag cggtggcaag
     7501 caggtgctgc ctatgcaggt gacccagcat gcccgggccc tggtcaaccc cgtaaagctg
     7561 agaaaactgg ttatccagcc agtaaacctg ctggtgagca tccatgcgtc gctgaagtta
     7621 tatatcgcca gcgaccatac cccgctgagc ttctctgtgt ttgagcgagg acccatcttt
     7681 accacagcca gacagctggt gcacgccctg gcaatgcact acgccgccgg cgccctcttt
     7741 cgggctggct gggtggtagg cagcttggat atcctcggta gccctgccag cctggtgcga
     7801 tccatcggaa acggcgtggc tgacttcttc cgactgccct acgaaggcct gaccagaggc
     7861 cctggcgcct tcgttagtgg cgttagcaga ggcactacct cattcgtgaa gcacatctct
     7921 aagggaaccc ttacttcaat caccaactta gccacatctc tcgcaagaaa catggacaga
     7981 cttagcctcg atgaggaaca ctacaaccgg caggaggagt ggcggagaca gctgcctgag
     8041 tcgctggggg agggactgag gcagggcctg tctcggttag gcatcagcct gttaggagcc
     8101 atcgcgggaa tcgtggacca gcctatgcag aattttcaga agacttctga agcccaggcc
     8161 agtgccggcc acaaggctaa gggagtcatc agcggcgttg gcaaggggat catgggcgtt
     8221 ttcactaaac caatcggggg cgcagccgag ctggtgagtc agacaggcta cggcattctg
     8281 cacggcgcag gcctgtctca gctgcctaaa caacggcatc agccatctga cctgcacgcc
     8341 gaccaggctc ccaacagcca cgtgaagtac gtgtggaaga tgctgcagag cctgggcaga
     8401 cctgaggtgc atatggccct ggacgtggtg ctcgtgcggg gcagcggtca ggaacacgag
     8461 ggctgcctgc tgttaacctc cgaggtgctg ttcgtcgtga gcgtatcgga ggatacccag
     8521 cagcaagctt ttcctgtgac cgagatcgac tgcgcccagg attctaagca gaacaactta
     8581 ctgacagtgc aactgaagca gcctagagtg gcctgtgacg tagaagtcga cggtgtgaga
     8641 gagagattgt ccgaacagca gtacaaccgg ctggtggact acattaccaa gacgagctgc
     8701 cacctggccc ctagctgtag cagtatgcag atcccctgtc ctgttgtggc cgccgaaccc
     8761 cccccctcca cggtgaagac ttaccactat ctggtcgacc cacatttcgc ccaggtgttc
     8821 ctttccaagt tcaccatggt gaaaaacaag gccctcagaa agggctttcc ctgaaccggt
     8881 tagtaatgag tttgatatct cgacaatcaa cctctggatt acaaaatttg tgaaagattg
     8941 actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct
     9001 ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg
     9061 ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact
     9121 gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc
     9181 gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc
     9241 cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaag
     9301 ctgacgtcct ttccatggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc
     9361 ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg
     9421 gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg
     9481 gccgcctccc cgcctggaaa cgggggaggc taactgaaac acggaaggag acaataccgg
     9541 aaggaacccg cgctatgacg gcaataaaaa gacagaataa aacgcacggg tgttgggtcg
     9601 tttgttcata aacgcggggt tcggtcccag ggctggcact ctgtcgatac cccaccgaga
     9661 ccccattggg gccaatacgc ccgcgtttct tccttttccc caccccaccc cccaagttcg
     9721 ggtgaaggcc cagggctcgc agccaacgtc ggggcggcag gccctgccat agcagatctg
     9781 cgcagctggg gctctagggg gtatccccac gcgccctgta gcggcgcatt aagcgcggcg
     9841 ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct
     9901 ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat
     9961 cggggcatcc ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt
    10021 gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg
    10081 acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac
    10141 cctatctcgg tctattcttt tgatttataa gggattttgg ggatttcggc ctattggtta
    10201 aaaaatgagc tgatttaaca aaaatttaac gcgaattaat tctgtggaat gtgtgtcagt
    10261 tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca
    10321 attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa
    10381 gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc
    10441 taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg
    10501 cagaggccga ggccgcctct gcctctgagc tattccagaa gtagtgagga ggcttttttg
    10561 gaggcctagg cttttgcaaa aagctcccgg gagcttgtat atccattttc ggatctgatc
    10621 aagagacagg atgaggatcg tttcgcatga ttgaacaaga tggattgcac gcaggttctc
    10681 cggccgcttg ggtggagagg ctattcggct atgactgggc acaacagaca atcggctgct
    10741 ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg
    10801 acctgtccgg tgccctgaat gaactgcagg acgaggcagc gcggctatcg tggctggcca
    10861 cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac tgaagcggga agggactggc
    10921 tgctattggg cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct cctgccgaga
    10981 aagtatccat catggctgat gcaatgcggc ggctgcatac gcttgatccg gctacctgcc
    11041 cattcgacca ccaagcgaaa catcgcatcg agcgagcacg tactcggatg gaagccggtc
    11101 ttgtcgatca ggatgatctg gacgaagagc atcaggggct cgcgccagcc gaactgttcg
    11161 ccaggctcaa ggcgcgcatg cccgacggcg aggatctcgt cgtgacccat ggcgatgcct
    11221 gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg attcatcgac tgtggccggc
    11281 tgggtgtggc ggaccgctat caggacatag cgttggctac ccgtgatatt gctgaagagc
    11341 ttggcggcga atgggctgac cgcttcctcg tgctttacgg tatcgccgct cccgattcgc
    11401 agcgcatcgc cttctatcgc cttcttgacg agttcttctg agcgggactc tggggttcgc
    11461 gaaatgaccg accaagcgac gcccaacctg ccatcacgag atttcgattc caccgccgcc
    11521 ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg ccggctggat gatcctccag
    11581 cgcggggatc tcatgctgga gttcttcgcc caccccaact tgtttattgc agcttataat
    11641 ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat
    11701 tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctgtat accgtcgacc
    11761 tctagctaga gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg
    11821 ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa
    11881 tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac
    11941 ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt
    12001 gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
    12061 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
    12121 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
    12181 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
    12241 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
    12301 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
    12361 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
    12421 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
    12481 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
    12541 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
    12601 tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag
    12661 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
    12721 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
    12781 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
    12841 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
    12901 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta
    12961 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
    13021 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg
    13081 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga
    13141 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt
    13201 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
    13261 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
    13321 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc
    13381 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca
    13441 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag
    13501 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
    13561 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
    13621 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
    13681 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
    13741 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
    13801 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
    13861 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
    13921 ccccgaaaag tgccacctga cgtcgacgga tcgggagatc tcccgatccc ctatggtcga
    13981 ctctcagtac aatctgctct gatgccgcat agttaagcca gtatctgctc cctgcttgtg
    14041 tgttggaggt cgctgagtag tgcgcgagca aaatttaagc tacaacaagg caaggcttga
    14101 ccgacaattg catgaagaat ctgcttaggg ttaggcgttt tgcgctgctt cgcgatgtac
    14161 gggccagata tacgcgttga cattgattat tgactagtta ttaatagtaa tcaattacgg
    14221 ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg gtaaatggcc
    14281 cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca
    14341 tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg
    14401 cccacttggc agtacatcaa gtgtatcata tgccaagtac gccccctatt gacgtcaatg
    14461 acggtaaatg gcccgcctgg cattatgccc agtacatgac cttatgggac tttcctactt
    14521 ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt tggcagtaca
    14581 tcaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac cccattgacg
    14641 tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt cgtaacaact
    14701 ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat ataagcagag
    14761 ctcgtttagt gaaccgtcag atcgcctgga gacgccatcc acgctgtttt gacctccata
    14821 gaagacaccg ggaccgatcc agcctccgga ctctagagcc accatgctcg agtcttacgt
    14881 tacacccatc ctcatgagtt acgtaaatag atacatcaaa aatctgaagc cttctgactt
    14941 acagctgagt ttgtggggcg gcgacgtggt cctgtcgaag ctagaattga agcttgacgt
    15001 gctagaacag gaactgaaac tccccttcac cttcttgtcg ggccacatcc acgagctcag
    15061 gatacatgtg ccatggacca agctgggtag cgagcctgtg gtcattacaa tcaacactat
    15121 ggaatgtatc ctcaagctga aggatggcat ccaagacgac cacgagagct gcggcagtaa
    15181 ctctaccaac agaagcacag ccgagtcgac caagagctct atcaagccac ggcggatgca
    15241 gcaggccgcc cccactgacc ccgacctccc gcctggttat gtacagtccc tgatcagacg
    15301 agtggtgaac aacgtgaaca tcgtgatcaa caacctaatc ctgaagtacg tggaagacga
    15361 cattgttctg tctgtgaaca tcacatctgc cgaatgctac accgtggggg aactgtggga
    15421 ccgggctttc atggacataa gtgccacaga cctggtgctc cgcaaggtga taaatttcag
    15481 cgactgcacc gtgtgtcttg ataagagaaa tgcctctggc aaaatcgagt tctaccaaga
    15541 tcccctcctg tacaagtgct ctttcagaac tagactacac ttcacctacg agaatttgaa
    15601 cagcaaaatg ccctcagtca ttaagatcca cacactggtg gaatcgctaa agctgagtat
    15661 caccgaccaa caattaccta tgtttatcag gatcatgcag ctgggaatcg ccctgtacta
    15721 cggtgagatc ggcaacttca aggagggcga gatcgaggac ctgacatgtc acaacaagga
    15781 tatgctgggc aacatcaccg gatctgagga cgagactcgg atcgacatgc agtaccccgc
    15841 ccagcacaag ggccaggaat tgtactctca acaggatgaa gagcaaccgc agggatgggt
    15901 ttcctgggct tggtcattcg tgcccgccat cgtctcttat gatgacggag aggaagactt
    15961 cgtcggaaat gaccctgcct caaccatgca ccagcagaaa gcccagaccc tgaaggaccc
    16021 tattgtgtcc ataggctttt actgtaccaa agccaccgtc accttcaagt taacggagat
    16081 gcaagtggaa tctagttact atagccctca gaaggtgaaa agcaaggaag tattatgttg
    16141 ggaacaggaa ggcaccaccg tagaggccct catgatgggc gagccctttt tcgattgtca
    16201 aatcggcttc gtgggatgca gggccatgtg cctaaagggc atcatgggcg tgaaagactt
    16261 tgaagagaac atgaatagat cagaaacaga agcctgcttc ttcatttgcg gcgataattt
    16321 gtccacaaaa ggctttacat acctgaccaa ctccctcttt gactatagaa gccctgagaa
    16381 caacggcacc agagctgagt tcatactcga tagtacccat cacaaagaga catacaccga
    16441 aatcgccggc atgcagcggt tcggcgcctt ttacatggac tacctgtaca ccatggaaaa
    16501 caccagcgga aaaggtagca caaaccagca ggactttagc tccggaaagt ccgaagacct
    16561 cggtacagtc caggagaaga gcacaaagag cctagtaatc ggccctctgg acttccggct
    16621 cgactcctct gccgtgcaca gaatcctgaa aatgatcgtc tgtgccctgg agcacgagta
    16681 cgaaccttac tctcggctta aaagcgacat taaggacgag aacgagacca tcctgaaccc
    16741 tgaggaggtg gcgctgttag aagagtacat ccctaccaga cacacttctg tgacactact
    16801 gaagtgcacc tgtacaatca gtatggctga attcaatctg ctggaccacc tgctgcccgt
    16861 catcatgggc gagaagaact cctccaattt catgaatact accaatttcc agagcctgag
    16921 accccttcct agcatcagaa ttctagttga taagatcaac ctagagcact ctgtgccaat
    16981 gtacgccgag cagcttgtcc acgtggtatc ttcactcacc cagccttcag ataacctgct
    17041 gcattactgt tacgttcact gctacctgaa gatcttcggc ttccaagcag gcctgacatc
    17101 gctcgactgc tctggatcat actgcctccc agtccccgtg atccccagtt tttcgaccgc
    17161 tctgtacgga aagttactga agctgccaac ctgttggacc aagcgtagtc agatcgccat
    17221 caccgaagga atcttcgagc tgcctaacct gacaatccag gccaccagag ctcagacgct
    17281 gttgctccaa gccatctacc agtcttggtc ccacctgggc aacgtgagct cctctgccgt
    17341 gatcgaggcc ctgatcaacg agatcttttt gagtatcggc gtgaaaagca agaaccctct
    17401 gcctacactg gaagggagca tccagaacgt ggagctgaaa tactgttcaa caagccttgt
    17461 gaaatgcgcc agcgggacca tgggaagcat caagatttgc gctaaagctc cggtggacag
    17521 cggcaaggaa aaactgatac cactgctgca gggcccgagc gacaccaaag acctccacag
    17581 tactaagtgg ctgaatgaga gccgaaaacc tgagagtctc cttgcccccg atctgatggc
    17641 cttcaccatc caggtgcccc aatacatcga ctactgccac aattcagggg ctgtgttact
    17701 gtgcagcatt cagggactgg ctgtgaacat cgaccccatt ctgtacacct ggctcatcta
    17761 tcagccacaa aagagaacca gtcgtcacat gcagcagcag cccgtggtag ccgtgcctct
    17821 cgtgatgcca gtgtgtcgcc ggaaagagga cgaggtgtct atcggcagcg ctcctctcgc
    17881 aaagcagcag tcttaccagg cctcggagta cgcctctagc cctgtgaaga ccaagaccgt
    17941 cactgaatct cgaccccttt cggtccccgt gaaagccatg cttaacatct ctgagtcctg
    18001 cagaagtccg gaggagcgga tgaaggaatt catcggtatt gtgtggaatg ccgtgaaaca
    18061 tctcacactg caactcgagg tgcaaagctg ctgtgtgttc atccccaacg actctttgcc
    18121 tagcccttcc accatcgtgt ctggcgatat cccggggact gtccggagct ggtaccacgg
    18181 ccagaccagt atgcccggca ctctagtact ctgtcttcca cagattaaaa ttatctctgc
    18241 cggacacaag tacatggaac ctctccagga aatcccattc gtgatcccta gacctatcct
    18301 ggaagaaggc gatgcgttcc cttggacaat ctccctccat aatttttcga tctatacact
    18361 gctgggaaag caggttacgc tgtgccttgt ggagccaatg ggttgcacat ctaccctggc
    18421 cgtgacctcg cagaaactgc ttgccaccgg accagacacc cggcactcat tcgtagtctg
    18481 cttgcatgtg gacttagagt ccctcgaaat caaatgctct aacccgcagg tgcagctgtt
    18541 ttacgaactg accgatatta tgaacaaggt gtggaacaaa atacagaaac ggggcaacct
    18601 taacctgagc ccaactagcc cagagacaat ggctgggcct gtgcctacga gtcccgtgag
    18661 atccagcatc ggtaccgccc ctcctgacac ttctacctgt tcgccttccg ctgacatcgg
    18721 cacaactacc gagggggaca gtatacaggc tggcgaggag tctcctggct ctggctcttc
    18781 aggatccggc atggcagaaa tcggtactgg ctttccattc gacccccatt atgtggaagt
    18841 cctgggcgag cgcatgcact acgtcgatgt tggtccgcgc gatggcaccc ctgtgctgtt
    18901 cctgcacggt aacccgacct cctcctacgt gtggcgcaac atcatcccgc atgttgcacc
    18961 gacccatcgc tgcattgctc cagacctgat c
//
