LOCUS       sequence-449635-       18817 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  codon optimized GFP tagged VPS13B for expression in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 18817)
  AUTHORS   Ugur B, Schueder F, Shin J, Hanna MG, Wu Y, Leonzino M, Su M, McAdow
            AR, Wilson C, Postlethwait J, Solnica-Krezel L, Bewersdorf J, De 
            Camilli P
  TITLE     VPS13B is localized at the cis-trans Golgi complex interface and is 
            a functional partner of FAM177A1.
  JOURNAL   bioRxiv [Preprint]. 2023 Dec 18:2023.12.18.572081. doi: 
            10.1101/2023.12.18.572081.
  PUBMED    38187698
REFERENCE   2  (bases 1 to 18817)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            [Preprint]. 2023 Dec 18:2023.12.18.572081. doi: 
            10.1101/2023.12.18.572081."
COMMENT     Sequence Label: VPS13B^GFP
FEATURES             Location/Qualifiers
     source          1..18817
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(133..153)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        138..467
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      318..453
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     380..399
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             534..1328
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(588..607)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     1198..1217
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    1504..1625
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1541..1560)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1595..1614
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(1674..1690)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1674..1690)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1687..1709)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1698..1714
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1722..1728,1729..1746,1747..1752))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1767..1788
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1905..1922)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2076..2664)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2156..2175)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2835..3626,3627..3695))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3458..3477
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3696..3800)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(3875..3894)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        4066..4445
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4446..4649
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4600..4620
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     4646..4670
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             join(8678..8680,8681..8683,8684..9394)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(8678..8680,8681..8683,8684..9394)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(8723..8744)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     9331..9352
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    17609..18197
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(17662..17682)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(18080..18091)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(18219..18238)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    18263..18311
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      join(18513..18817,1..124)
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(18600..18619)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     join(18810..18817,1..14)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg
       61 attttgggga tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg
      121 aattaattct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag
      181 gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag
      241 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc
      301 cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc
      361 atggctgact aatttttttt atttatgcag aggccgaggc cgcctctgcc tctgagctat
      421 tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctcccgggag
      481 cttgtatatc cattttcgga tctgatcaag agacaggatg aggatcgttt cgcatgattg
      541 aacaagatgg attgcacgca ggttctccgg ccgcttgggt ggagaggcta ttcggctatg
      601 actgggcaca acagacaatc ggctgctctg atgccgccgt gttccggctg tcagcgcagg
      661 ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa ctgcaggacg
      721 aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg
      781 ttgtcactga agcgggaagg gactggctgc tattgggcga agtgccgggg caggatctcc
      841 tgtcatctca ccttgctcct gccgagaaag tatccatcat ggctgatgca atgcggcggc
      901 tgcatacgct tgatccggct acctgcccat tcgaccacca agcgaaacat cgcatcgagc
      961 gagcacgtac tcggatggaa gccggtcttg tcgatcagga tgatctggac gaagagcatc
     1021 aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc gcgcatgccc gacggcgagg
     1081 atctcgtcgt gacccatggc gatgcctgct tgccgaatat catggtggaa aatggccgct
     1141 tttctggatt catcgactgt ggccggctgg gtgtggcgga ccgctatcag gacatagcgt
     1201 tggctacccg tgatattgct gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc
     1261 tttacggtat cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt
     1321 tcttctgagc gggactctgg ggttcgcgaa atgaccgacc aagcgacgcc caacctgcca
     1381 tcacgagatt tcgattccac cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc
     1441 cgggacgccg gctggatgat cctccagcgc ggggatctca tgctggagtt cttcgcccac
     1501 cccaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc
     1561 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta
     1621 tcttatcatg tctgtatacc gtcgacctct agctagagct tggcgtaatc atggtcatag
     1681 ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc
     1741 ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc
     1801 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa
     1861 cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg
     1921 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
     1981 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
     2041 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
     2101 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
     2161 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
     2221 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
     2281 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
     2341 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
     2401 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
     2461 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca
     2521 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
     2581 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
     2641 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
     2701 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
     2761 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
     2821 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
     2881 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
     2941 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat
     3001 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
     3061 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
     3121 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt
     3181 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
     3241 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
     3301 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
     3361 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
     3421 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga
     3481 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
     3541 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
     3601 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
     3661 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga
     3721 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
     3781 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt cgacggatcg
     3841 ggagatctcc cgatccccta tggtcgactc tcagtacaat ctgctctgat gccgcatagt
     3901 taagccagta tctgctccct gcttgtgtgt tggaggtcgc tgagtagtgc gcgagcaaaa
     3961 tttaagctac aacaaggcaa ggcttgaccg acaattgcat gaagaatctg cttagggtta
     4021 ggcgttttgc gctgcttcgc gatgtacggg ccagatatac gcgttgacat tgattattga
     4081 ctagttatta atagtaatca attacggggt cattagttca tagcccatat atggagttcc
     4141 gcgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac ccccgcccat
     4201 tgacgtcaat aatgacgtat gttcccatag taacgccaat agggactttc cattgacgtc
     4261 aatgggtgga gtatttacgg taaactgccc acttggcagt acatcaagtg tatcatatgc
     4321 caagtacgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt
     4381 acatgacctt atgggacttt cctacttggc agtacatcta cgtattagtc atcgctatta
     4441 ccatggtgat gcggttttgg cagtacatca atgggcgtgg atagcggttt gactcacggg
     4501 gatttccaag tctccacccc attgacgtca atgggagttt gttttggcac caaaatcaac
     4561 gggactttcc aaaatgtcgt aacaactccg ccccattgac gcaaatgggc ggtaggcgtg
     4621 tacggtggga ggtctatata agcagagctc gtttagtgaa ccgtcagatc gcctggagac
     4681 gccatccacg ctgttttgac ctccatagaa gacaccggga ccgatccagc ctccggactc
     4741 tagagccacc atgctcgagt cttacgttac acccatcctc atgagttacg taaatagata
     4801 catcaaaaat ctgaagcctt ctgacttaca gctgagtttg tggggcggcg acgtggtcct
     4861 gtcgaagcta gaattgaagc ttgacgtgct agaacaggaa ctgaaactcc ccttcacctt
     4921 cttgtcgggc cacatccacg agctcaggat acatgtgcca tggaccaagc tgggtagcga
     4981 gcctgtggtc attacaatca acactatgga atgtatcctc aagctgaagg atggcatcca
     5041 agacgaccac gagagctgcg gcagtaactc taccaacaga agcacagccg agtcgaccaa
     5101 gagctctatc aagccacggc ggatgcagca ggccgccccc actgaccccg acctcccgcc
     5161 tggttatgta cagtccctga tcagacgagt ggtgaacaac gtgaacatcg tgatcaacaa
     5221 cctaatcctg aagtacgtgg aagacgacat tgttctgtct gtgaacatca catctgccga
     5281 atgctacacc gtgggggaac tgtgggaccg ggctttcatg gacataagtg ccacagacct
     5341 ggtgctccgc aaggtgataa atttcagcga ctgcaccgtg tgtcttgata agagaaatgc
     5401 ctctggcaaa atcgagttct accaagatcc cctcctgtac aagtgctctt tcagaactag
     5461 actacacttc acctacgaga atttgaacag caaaatgccc tcagtcatta agatccacac
     5521 actggtggaa tcgctaaagc tgagtatcac cgaccaacaa ttacctatgt ttatcaggat
     5581 catgcagctg ggaatcgccc tgtactacgg tgagatcggc aacttcaagg agggcgagat
     5641 cgaggacctg acatgtcaca acaaggatat gctgggcaac atcaccggat ctgaggacga
     5701 gactcggatc gacatgcagt accccgccca gcacaagggc caggaattgt actctcaaca
     5761 ggatgaagag caaccgcagg gatgggtttc ctgggcttgg tcattcgtgc ccgccatcgt
     5821 ctcttatgat gacggagagg aagacttcgt cggaaatgac cctgcctcaa ccatgcacca
     5881 gcagaaagcc cagaccctga aggaccctat tgtgtccata ggcttttact gtaccaaagc
     5941 caccgtcacc ttcaagttaa cggagatgca agtggaatct agttactata gccctcagaa
     6001 ggtgaaaagc aaggaagtat tatgttggga acaggaaggc accaccgtag aggccctcat
     6061 gatgggcgag ccctttttcg attgtcaaat cggcttcgtg ggatgcaggg ccatgtgcct
     6121 aaagggcatc atgggcgtga aagactttga agagaacatg aatagatcag aaacagaagc
     6181 ctgcttcttc atttgcggcg ataatttgtc cacaaaaggc tttacatacc tgaccaactc
     6241 cctctttgac tatagaagcc ctgagaacaa cggcaccaga gctgagttca tactcgatag
     6301 tacccatcac aaagagacat acaccgaaat cgccggcatg cagcggttcg gcgcctttta
     6361 catggactac ctgtacacca tggaaaacac cagcggaaaa ggtagcacaa accagcagga
     6421 ctttagctcc ggaaagtccg aagacctcgg tacagtccag gagaagagca caaagagcct
     6481 agtaatcggc cctctggact tccggctcga ctcctctgcc gtgcacagaa tcctgaaaat
     6541 gatcgtctgt gccctggagc acgagtacga accttactct cggcttaaaa gcgacattaa
     6601 ggacgagaac gagaccatcc tgaaccctga ggaggtggcg ctgttagaag agtacatccc
     6661 taccagacac acttctgtga cactactgaa gtgcacctgt acaatcagta tggctgaatt
     6721 caatctgctg gaccacctgc tgcccgtcat catgggcgag aagaactcct ccaatttcat
     6781 gaatactacc aatttccaga gcctgagacc ccttcctagc atcagaattc tagttgataa
     6841 gatcaaccta gagcactctg tgccaatgta cgccgagcag cttgtccacg tggtatcttc
     6901 actcacccag ccttcagata acctgctgca ttactgttac gttcactgct acctgaagat
     6961 cttcggcttc caagcaggcc tgacatcgct cgactgctct ggatcatact gcctcccagt
     7021 ccccgtgatc cccagttttt cgaccgctct gtacggaaag ttactgaagc tgccaacctg
     7081 ttggaccaag cgtagtcaga tcgccatcac cgaaggaatc ttcgagctgc ctaacctgac
     7141 aatccaggcc accagagctc agacgctgtt gctccaagcc atctaccagt cttggtccca
     7201 cctgggcaac gtgagctcct ctgccgtgat cgaggccctg atcaacgaga tctttttgag
     7261 tatcggcgtg aaaagcaaga accctctgcc tacactggaa gggagcatcc agaacgtgga
     7321 gctgaaatac tgttcaacaa gccttgtgaa atgcgccagc gggaccatgg gaagcatcaa
     7381 gatttgcgct aaagctccgg tggacagcgg caaggaaaaa ctgataccac tgctgcaggg
     7441 cccgagcgac accaaagacc tccacagtac taagtggctg aatgagagcc gaaaacctga
     7501 gagtctcctt gcccccgatc tgatggcctt caccatccag gtgccccaat acatcgacta
     7561 ctgccacaat tcaggggctg tgttactgtg cagcattcag ggactggctg tgaacatcga
     7621 ccccattctg tacacctggc tcatctatca gccacaaaag agaaccagtc gtcacatgca
     7681 gcagcagccc gtggtagccg tgcctctcgt gatgccagtg tgtcgccgga aagaggacga
     7741 ggtgtctatc ggcagcgctc ctctcgcaaa gcagcagtct taccaggcct cggagtacgc
     7801 ctctagccct gtgaagacca agaccgtcac tgaatctcga cccctttcgg tccccgtgaa
     7861 agccatgctt aacatctctg agtcctgcag aagtccggag gagcggatga aggaattcat
     7921 cggtattgtg tggaatgccg tgaaacatct cacactgcaa ctcgaggtgc aaagctgctg
     7981 tgtgttcatc cccaacgact ctttgcctag cccttccacc atcgtgtctg gcgatatccc
     8041 ggggactgtc cggagctggt accacggcca gaccagtatg cccggcactc tagtactctg
     8101 tcttccacag attaaaatta tctctgccgg acacaagtac atggaacctc tccaggaaat
     8161 cccattcgtg atccctagac ctatcctgga agaaggcgat gcgttccctt ggacaatctc
     8221 cctccataat ttttcgatct atacactgct gggaaagcag gttacgctgt gccttgtgga
     8281 gccaatgggt tgcacatcta ccctggccgt gacctcgcag aaactgcttg ccaccggacc
     8341 agacacccgg cactcattcg tagtctgctt gcatgtggac ttagagtccc tcgaaatcaa
     8401 atgctctaac ccgcaggtgc agctgtttta cgaactgacc gatattatga acaaggtgtg
     8461 gaacaaaata cagaaacggg gcaaccttaa cctgagccca actagcccag agacaatggc
     8521 tgggcctgtg cctacgagtc ccgtgagatc cagcatcggt accgcccctc ctgacacttc
     8581 tacctgttcg ccttccgctg acatcggcac aactaccgag ggggacagta tacaggctgg
     8641 cgaggagtct cctggctctg gctcttcagg atccggcatg gtgagcaagg gcgaggagct
     8701 gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt
     8761 cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat
     8821 ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc tgacctacgg
     8881 cgtgcagtgc ttcagccgct accccgacca catgaagcag cacgacttct tcaagtccgc
     8941 catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa
     9001 gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg
     9061 catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag
     9121 ccacaacgtc tatatcatgg ccgacaagca gaagaacggc atcaaggtga acttcaagat
     9181 ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc
     9241 catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagcaccc agtccgccct
     9301 gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc
     9361 cgggatcact ctcggcatgg acgagctgta caagggcggc ggatccggcg gattcagtga
     9421 tagcgtgacc cttgaacaga ccacttccaa tatcggtggt accagtggcc gggtgtctct
     9481 gtggatgcag tgggtcttgc ctaaaatcac aattaagctg ttcgcccctg accccgagaa
     9541 taagggtacc gaagtgtgca tggtgtctga actggaggac ctgtccgcca gcattgacgt
     9601 gcaggacgtg tacaccaagg tcaagtgcaa gatcgagtcc tttaacatag accactaccg
     9661 ctcttctctg ggggaggaat gctggtccct gggccagtgt ggcggcgtgt ttcttagctg
     9721 caccgataag ctcaacagac gcaccctcct tgtgagacct atctccaagc aagacccctt
     9781 cagtaactgc tccggatttt ttcctagcac caccacaaag ctgctggacg gaacccatca
     9841 gcaacacggc ttcttatctc tgacctacac caaggctgtc accaagaacg tgagacataa
     9901 gctgaccagc cggaatgaga gaagatcttt ccacaagcta tccgaagggc tcatggacgg
     9961 gtccccccac ttcctgcacg aaatactgct ctccgcccaa gccttcgaca tagttctgta
    10021 ctttccccta ctgaacgcca tcgccagcat cttccaggcc aagctgccta agacacaaaa
    10081 ggaaaagcgg aagagccccg gacagccaat gcggactcac acactcacca gcagaaacct
    10141 gcccctcatc tacgtgaata cttcggtgat cagaatcttt atccccaaga cagaagagat
    10201 gcagccgacc gtggaagcca accaggccgc caaagaagat acagtggttc taaaaatcgg
    10261 ctctgttgca atggccccgc aggccgacaa ccctctggga agaagcgtgc tgagaaagga
    10321 tatctatcag cgggccctga acctgggcat actgagagac cctggaagcg aaatcgaaga
    10381 ccgacagtac cagatcgatc tgcagagcat caacatcggc acggcccaat ggcaccagct
    10441 gaagcctgag aaggagtctg tgtccggggg cgtcgttaca gagaccgaaa gaaactccca
    10501 aaaccccgcc ctggagtgga acatggcgag cagcataagg cggcaccagg agagaagagc
    10561 catcctgacc cctgtgctca cagatttctc agtaagaatc accggcgctc ccgcagtgat
    10621 ttttactaaa gtggtatctc ctgaaaacct ccatacagaa gaaatcctcg tctgcggcca
    10681 cagtctcgag gtgaatatca ctaccaacct cgacttcttc ctgtctgtcg cacaggtcca
    10741 gctgctgcac cagctgattg tggccaatat gacaggcctg gaacccagca acaaggctgc
    10801 cgaaatttcc aagcaggaac agaagaaggt cgatatattt gacggaggca tggccgagac
    10861 aagcagccgg tacagtggcg cacaggacag cggcattgga tctgatagtg tcaagatcag
    10921 aatagtgcag atcgagcagc acagcggcgc ctctcagcat aggattgcaa gaccgtccag
    10981 acagtcaagc atcgtgaaaa acctgaactt catccccttt gacattttca tcaccgcctc
    11041 aagaatcagc ctgatgacct atagctgcat ggctttgagc aagtcaaagt cccaagaaca
    11101 gaagaacaac gaaaagacag acaaatctag cctgaaccta cccgaagtag atagcgatgt
    11161 ggccaagcct aatcaggcct gcatcagcac agtcacagct gaggatctac tgagatcctc
    11221 catctctttt cctagtggta aaaaaatcgg ggtcttatca ttggaatcgc tgcacgcctc
    11281 cactagaagc tctgcacggc aggctctagg catcacaatc gtgagacagc ctggcagaag
    11341 aggtacaggc gacctgcagc tcgagccctt cctttatttc atcgtgtccc agcctagtct
    11401 cctgctgtcg tgccatcaca gaaagcagag agtggaggtt tccatcttcg acgccgttct
    11461 aaagggcgtc gcctctgact acaagtgtat tgacccggga aaaaccctgc ccgaggcact
    11521 tgattactgt accgtgtggc tgcaaaccgt gcccggcgag attgactcca agtccggaat
    11581 ccctcccagt ttcattacac tgcaaatcaa agatttcctc aacggccctg ccgacgtgaa
    11641 cctggacatc agtaagcctc tcaaagcaaa cctgagtttc acaaagctgg atcagatcaa
    11701 cctgtttctg aagaaaataa agaatgccca ctcactcgct cactctgagg aaacctctgc
    11761 catgtctaat accatggtga acaaagacga tctgcccgtg tcaaagtact atagaggcaa
    11821 acttagtaaa cctaaaattc acggcgatgg agtacaaaag atctcggccc aggaaaatat
    11881 gtggcgagct gtctcctgct tccagaaaat ttccgttcag acgacccaga tcgtcatcag
    11941 tatggaaacc gttcctcaca cctcgaagcc ttgtctgctc gcttctctgt ccaatctgaa
    12001 cggctccctg tcagtgaagg ccacacagaa agtgcccgga atcattctgg gctcgtcttt
    12061 cctgctgtca atcaacgact tcctgctgaa gacctccctt aaggaacgga gcagaatcct
    12121 gattgggcca tgctgcgcca cagcgaacct ggaagctaaa tggtgcaagc actctggaaa
    12181 tcctggtcct gagcaatcta tcccaaagat cagtatcgac cttaggggcg gcctgctgca
    12241 agtgttctgg ggacaggagc acctcaattg cctggtgctg ctgcacgagt tactgaatgg
    12301 gtacctaaat gaggagggca acttcgaggt acaggtgagt gagcctgtgc cacagatgag
    12361 cagcccggtt gagaagaatc agacattcaa gagcgagcag agctcagatg atctcagaac
    12421 tggcctgttc cagtacgttc aggatgccga gagcctgaag ctgcctggag tgtacgaagt
    12481 tctattctac aacgagactg aagactgccc cggcatgatg ctctggcggt acccagaacc
    12541 tagagtgctg accctggtgc gcatcacacc agtgccattc aacaccaccg aagatcctga
    12601 tatcagtacc gccgacctgg gggatgtgct gcaggtccct tgcagcctcg aatactggga
    12661 tgagctgcag aaggtattcg tggcctttag agagttcaat ctgtctgaat caaaggtgtg
    12721 cgagctgcaa ttacctgaca tcaacctcgt taacgaccag aagaaactgg tttctagcga
    12781 cctgtggcgg atcgttctta acagctcaca aaacggcgcc gatgatcagt ccagtgcctc
    12841 ggaatctggc tcccagtcta cctgcgaccc tctcgtcact ccgaccgcgc tcgccgcctg
    12901 cacacgggtg gatagctgct ttaccccatg gttcgtgccc tctctgtgcg tgagcttcca
    12961 gttcgcccac ttggaatttc acttatgcca ccatctggat caactgggca ccgccgcccc
    13021 ccaatacctt cagcccttcg tgagcgaccg gaacatgcct agcgagttgg agtacatgat
    13081 tgtgtctttc agagagcccc atatgtacct gagacagtgg aacaacgggt ctgtatgcca
    13141 ggagatccaa ttcctggccc aggcggactg caagctcctg gagtgcagaa acgtgacaat
    13201 gcagagtgtg gtgaaacctt tttctatctt cggtcagatg gccgtgtcct ccgacgtggt
    13261 ggagaaactg ttagattgta ccgttatcgt agacagcgtc tttgtaaacc tgggccagca
    13321 tgtggttcat tcgttgaaca ctgcgatcca ggcttggcag caaaataagt gccctgaagt
    13381 ggaggaactg gtgttctcgc atttcgtgat ctgtaacgac acacaggaga ccctgagatt
    13441 cggccaggta gacaccgacg aaaatatttt gcttgcctct ctacacagtc accaatactc
    13501 ctggagatcc cacaagtccc ctcaactcct gcacatctgc atcgagggat ggggcaactg
    13561 gcggtggagc gagccattct ccgtggacca cgccggcact tttattcgga ccatacaata
    13621 tcgggggaga accgctagcc tcatcatcaa ggttcagcag ctgaacgggg tccagaagca
    13681 aatcattatc tgtggccgtc aaatcatctg ctcatacctg tctcagtcca tcgagctgaa
    13741 ggtggtgcag cactacattg gccaagatgg ccaggccgta gttcgggaac acttcgattg
    13801 cctgaccgcc aagcagaagc tgccttccta catcctggag aacaatgagc ttacagagct
    13861 gtgcgtgaag gcgaaggggg acgaggactg gtccagagat gtttgtttag aaagcaaagc
    13921 accggaatac tctatcgtaa tccaggtgcc atctagcaac tcgtctatca tctacgtgtg
    13981 gtgcaccgtg ctgacactgg agcccaacag ccaagtgcag caaagaatga tcgtgttttc
    14041 cccactgttc atcatgagat cccacctgcc agacccaatc atcatccacc tagaaaagag
    14101 atctctcggc ctgtccgaga ctcagatcat acctggtaag ggccaagaaa aaccactgca
    14161 gaatatcgag cctgatctgg tgcaccacct gaccttccag gctagagaag agtacgatcc
    14221 ctccgactgc gccgtcccaa tctctacctc tcttatcaag cagatcgcaa caaaagtgca
    14281 ccccggcggc accgtcaacc agatcctgga cgagttctac ggccctgaaa agagcctgca
    14341 gcccatctgg ccttacaaca aaaaggattc cgacagaaac gagcagctgt cacagtggga
    14401 cagtccaatg cgggtgaagt tatcaatctg gaaaccctac gtgcggacgt tactgataga
    14461 gctgttacct tgggcgttgc tgataaacga gtcaaagtgg gacctgtggc tgtttgaggg
    14521 cgagaaaatc gtgcttcaag taccagccgg caagatcatt atccctccaa acttccagga
    14581 ggcctttcag atcggcatct actgggccaa caccaacacc gtccataagt ctgtggccat
    14641 taagttagtg cataacttga cctctcctaa atggaaggac ggcggcaacg gagaggttgt
    14701 gaccctggac gaagaggcct tcgtggacac cgagatcaga cttggtgcat tccctggcca
    14761 ccagaagctg tgtcagttct gcatcagctc catggtccag cagggcatcc agatcattca
    14821 gattgaagac aagacaacca ttatcaacaa cacaccctac cagatcttct acaaacctca
    14881 gttatccgtg tgtaaccctc acagcggcaa ggaatatttc cgagtgcctg attctgccac
    14941 gttctcaatc tgccctggcg gagagcagcc tgctatgaag tcctcgtccc tgccttgctg
    15001 ggatctgatg ccagacatct cccagagcgt actcgacgcc agtctcctac agaaacagat
    15061 catgctgggt ttcagtccag ctccaggcgc cgacagcagc caatgttgga gccttcctgc
    15121 tatagttcgg cccgaattcc ctcggcagag cgtggccgtg cctttaggaa atttcagaga
    15181 aaacggcttt tgcacaagag ccattgtgct aacataccaa gagcacctgg gagtgactta
    15241 cctgacgctg agcgaagatc catctcccag agtcatcatc cacaacagat gccccgttaa
    15301 gatgctgatt aaggagaaca ttaaggacat cccgaagttc gaagtgtact gcaagaaaat
    15361 cccgtccgag tgcagcatac accatgaact ctaccaccag atttccagct accccgattg
    15421 caagacgaag gacctgctgc ccagcttgct gctgagagtg gaacctctgg acgaggtgac
    15481 tacagagtgg tctgacgcca tcgacatcaa cagccagggc acacaagtag ttttcttgac
    15541 aggatttggc tacgtctacg tcgatgtcgt tcaccagtgt ggcactgtgt tcattactgt
    15601 agcccctgaa gggaaggctg gccccattct caccaacacc aacagagccc cggaaaaaat
    15661 tgtgaccttt aagatgttca tcacacaact gtcattggct gtgtttgatg atctgaccca
    15721 ccacaaagcc agcgcggagc tcctgcgact caccttagat aacatctttc tgtgcgtcgc
    15781 cccaggcgct ggcccactgc ctggcgagga acccgtcgcc gccctgttcg agctgtattg
    15841 cgttgagatt tgctgcggcg acctacaact ggacaaccag ctgtacaaca aaagcaattt
    15901 ccactttgcc gtcctggttt gccagggcga aaaggctgaa ccaatccagt gtagcaagat
    15961 gcagagcctg cttatcagca acaaggaatt ggaggagtac aaggaaaagt gtttcatcaa
    16021 gctgtgcatt accctgaacg aaggcaaatc catcctctgc gatatcaatg aatttagctt
    16081 cgaactcaaa ccagccagac tgtacgttga ggatacattc gtgtactaca tcaagactct
    16141 cttcgacaca tacctgccta acagccgtct agccggacac agtacccacc tgagcggtgg
    16201 caagcaggtg ctgcctatgc aggtgaccca gcatgcccgg gccctggtca accccgtaaa
    16261 gctgagaaaa ctggttatcc agccagtaaa cctgctggtg agcatccatg cgtcgctgaa
    16321 gttatatatc gccagcgacc ataccccgct gagcttctct gtgtttgagc gaggacccat
    16381 ctttaccaca gccagacagc tggtgcacgc cctggcaatg cactacgccg ccggcgccct
    16441 ctttcgggct ggctgggtgg taggcagctt ggatatcctc ggtagccctg ccagcctggt
    16501 gcgatccatc ggaaacggcg tggctgactt cttccgactg ccctacgaag gcctgaccag
    16561 aggccctggc gccttcgtta gtggcgttag cagaggcact acctcattcg tgaagcacat
    16621 ctctaaggga acccttactt caatcaccaa cttagccaca tctctcgcaa gaaacatgga
    16681 cagacttagc ctcgatgagg aacactacaa ccggcaggag gagtggcgga gacagctgcc
    16741 tgagtcgctg ggggagggac tgaggcaggg cctgtctcgg ttaggcatca gcctgttagg
    16801 agccatcgcg ggaatcgtgg accagcctat gcagaatttt cagaagactt ctgaagccca
    16861 ggccagtgcc ggccacaagg ctaagggagt catcagcggc gttggcaagg ggatcatggg
    16921 cgttttcact aaaccaatcg ggggcgcagc cgagctggtg agtcagacag gctacggcat
    16981 tctgcacggc gcaggcctgt ctcagctgcc taaacaacgg catcagccat ctgacctgca
    17041 cgccgaccag gctcccaaca gccacgtgaa gtacgtgtgg aagatgctgc agagcctggg
    17101 cagacctgag gtgcatatgg ccctggacgt ggtgctcgtg cggggcagcg gtcaggaaca
    17161 cgagggctgc ctgctgttaa cctccgaggt gctgttcgtc gtgagcgtat cggaggatac
    17221 ccagcagcaa gcttttcctg tgaccgagat cgactgcgcc caggattcta agcagaacaa
    17281 cttactgaca gtgcaactga agcagcctag agtggcctgt gacgtagaag tcgacggtgt
    17341 gagagagaga ttgtccgaac agcagtacaa ccggctggtg gactacatta ccaagacgag
    17401 ctgccacctg gcccctagct gtagcagtat gcagatcccc tgtcctgttg tggccgccga
    17461 accccccccc tccacggtga agacttacca ctatctggtc gacccacatt tcgcccaggt
    17521 gttcctttcc aagttcacca tggtgaaaaa caaggccctc agaaagggct ttccctgaac
    17581 cggttagtaa tgagtttgat atctcgacaa tcaacctctg gattacaaaa tttgtgaaag
    17641 attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat
    17701 gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc
    17761 ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg
    17821 cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct
    17881 ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct
    17941 tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg
    18001 gaagctgacg tcctttccat ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac
    18061 gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct
    18121 gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct
    18181 ttgggccgcc tccccgcctg gaaacggggg aggctaactg aaacacggaa ggagacaata
    18241 ccggaaggaa cccgcgctat gacggcaata aaaagacaga ataaaacgca cgggtgttgg
    18301 gtcgtttgtt cataaacgcg gggttcggtc ccagggctgg cactctgtcg ataccccacc
    18361 gagaccccat tggggccaat acgcccgcgt ttcttccttt tccccacccc accccccaag
    18421 ttcgggtgaa ggcccagggc tcgcagccaa cgtcggggcg gcaggccctg ccatagcaga
    18481 tctgcgcagc tggggctcta gggggtatcc ccacgcgccc tgtagcggcg cattaagcgc
    18541 ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc
    18601 tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct
    18661 aaatcggggc atccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa
    18721 acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc
    18781 tttgacgttg gagtccacgt tctttaatag tggactc
//
