LOCUS       sequence-449642-        4603 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  AiE0724m is an enhancer sequence, designed to drive AAV-mediated 
            transgene expression in cortical Lamp5 GABAergic neurons.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4603)
  AUTHORS   Ben-Simon Y, Hooper M, Narayan S, Daigle TL, Dwivedi D, Way SW, 
            Oster A, Stafford DA, Mich JK, Taormina MJ, Martinez RA, Opitz-Araya
            X, Roth JR, Alexander JR, Allen S, Amster A, Arbuckle J, Ayala A, 
            Baker PM, Bakken TE, Barcelli T, Barta S, Bendrick J, Bertagnolli D,
            Bielstein C, Bishwakarma P, Bowlus J, Boyer G, Brouner K, Casian B, 
            Casper T, Chakka AB, Chakrabarty R, Chance RK, Chavan S, Clark M, 
            Colbert K, Collman F, Daniel S, Departee M, DiValentin P, Donadio N,
            Dotson N, Egdorf T, Fliss T, Gabitto M, Garcia J, Gary A, Gasperini 
            M, Gloe J, Goldy J, Gore BB, Graybuck L, Greisman N, Haeseleer F, 
            Halterman C, Haradon Z, Hastings SD, Helback O, Ho W, Hockemeyer D, 
            Huang C, Huff S, Hunker A, Johansen N, Jones D, Juneau Z, Kalmbach 
            B, Kannan M, Khem S, Kussick E, Kutsal R, Larsen R, Lee C, Lee AY, 
            Leibly M, Lenz GH, Li S, Liang E, Lusk N, Madigan Z, Malloy J, 
            Malone J, McCue R, Melchor J, Mollenkopf T, Moosman S, Morin E, 
            Newman D, Ng L, Ngo K, Omstead V, Otto S, Oyama A, Pena N, Pham T, 
            Phillips E, Pom CA, Potekhina L, Ransford S, Ray PL, Rette D, 
            Reynoldson C, Rimorin C, Rocha D, Ruiz A, Sanchez REA, Sawyer L, 
            Sedeno-Cortes A, Sevigny JP, Shapovalova N, Shepard N, Shulga L, 
            Sigler AR, Siverts L, Soliman S, Somasundaram S, Staats B, Stewart 
            K, Szelenyi E, Tieu M, Trader C, Tran A, van Velthoven CTJ, Walker 
            M, Wang Y, Weed N, Wirthlin M, Wood T, Wynalda B, Yao Z, Zhou T, 
            Ariza J, Dee N, Reding M, Ronellenfitch K, Mufti S, Sunkin SM, Smith
            KA, Esposito L, Waters J, Thyagarajan B, Yao S, Lein ES, Zeng H, 
            Levi BP, Ngai J, Ting JT, Tasic B
  TITLE     A suite of enhancer AAVs and transgenic mouse lines for genetic 
            access to cortical cell types.
  JOURNAL   Cell. 2025 May 16:S0092-8674(25)00513-6. doi: 
            10.1016/j.cell.2025.05.002.
  PUBMED    40403729
REFERENCE   2  (bases 1 to 4603)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2025 
            May 16:S0092-8674(25)00513-6. doi: 10.1016/j.cell.2025.05.002."
COMMENT     Sequence Label: AiP14668 - pAAV-AiE0724m-minBG-iCre(R297T)-BGH...
FEATURES             Location/Qualifiers
     source          1..4603
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      536..545
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             548..568
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    1634..1745
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   1863..2003
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   1863..1992
                     /label=AAV2 ITR
     rep_origin      2078..2533
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2165..2184)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2375..2396
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(2550..2569)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2669..2691
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(2729..2747)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2815..2919
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2920..2988,2989..3780)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3138..3157)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3951..4539
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4440..4459
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     repeat_region   join(4601..4603,1..127)
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
ORIGIN
        1 gcaggcagct gcgcgctcgc tcgctcactg aggccgcccg ggcgtcgggc gacctttggt
       61 cgcccggcct cagtgagcga gcgagcgcgc agagagggag tggccaactc catcactagg
      121 ggttcctgcg gccgcacgcg taccggtccc cttaccattt cttcaacata agggtaaagc
      181 atgtctccaa agtattccgt agcctcgatt ggaagctgtg ctggcaaatt gtcaatggaa
      241 cacatcagga tcccggagcc ttcaacacta agagaggcaa catcctttat tcaaatgcac
      301 aaaatatgac ttgaaatttt tcccagactc tactccattc caacatttgt atcactgcta
      361 ttaatatatt tatagtcttg agaaaaaggc ataacactat aaacaagact gcttttcaaa
      421 caaaactttt tttttccaac ttggcagcct atctgagctc gggctgggca taaaagtcag
      481 ggcagagcca tctattgctt acatttgctt ctgggatcca gatctttcga agctagccac
      541 catggtgccc aagaagaaga ggaaagtctc caacctgctg actgtgcacc aaaacctgcc
      601 tgccctccct gtggatgcca cctctgatga agtcaggaag aacctgatgg acatgttcag
      661 ggacaggcag gccttctctg aacacacctg gaagatgctc ctgtctgtgt gcagatcctg
      721 ggctgcctgg tgcaagctga acaacaggaa atggttccct gctgaacctg aggatgtgag
      781 ggactacctc ctgtacctgc aagccagagg cctggctgtg aagaccatcc aacagcacct
      841 gggccagctc aacatgctgc acaggagatc tggcctgcct cgcccttctg actccaatgc
      901 tgtgtccctg gtgatgagga gaatcagaaa ggagaatgtg gatgctgggg agagagccaa
      961 gcaggccctg gcctttgaac gcactgactt tgaccaagtc agatccctga tggagaactc
     1021 tgacagatgc caggacatca ggaacctggc cttcctgggc attgcctaca acaccctgct
     1081 gcgcattgcc gaaattgcca gaatcagagt gaaggacatc tcccgcaccg atggtgggag
     1141 aatgctgatc cacattggca ggaccaagac cctggtgtcc acagctggtg tggagaaggc
     1201 cctgtccctg ggggttacca agctggtgga gagatggatc tctgtgtctg gtgtggctga
     1261 tgaccccaac aactacctgt tctgccgggt cagaaagaat ggtgtggctg ccccttctgc
     1321 cacctcccaa ctgtccaccc gggccctgga agggatcttt gaggccaccc accgcctgat
     1381 ctatggtgcc aaggatgact ctgggcagag atacctggcc tggtctggcc actctgccag
     1441 agtgggtgct gccaccgaca tggccagggc tggtgtgtcc atccctgaaa tcatgcaggc
     1501 tggtggctgg accaatgtga acattgtgat gaactacatc agaaacctgg actctgagac
     1561 tggggccatg gtgaggctgc tcgaagatgg ggactgaggc gcgccgaatt caagcttctc
     1621 gagagatctt cgactgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg
     1681 ccttccttga ccctggaagg tgccactccc actgtccttt cctaataaaa tgaggaaatt
     1741 gcatcgcatt gtctgagtag gtgtcattct attctggggg gtggggtggg gcaggacagc
     1801 aagggggagg attgggaaga caatagcagg catgagatct cacgtgcgga ccgagcggcc
     1861 gcaggaaccc ctagtgatgg agttggccac tccctctctg cgcgctcgct cgctcactga
     1921 ggccgggcga ccaaaggtcg cccgacgccc gggctttgcc cgggcggcct cagtgagcga
     1981 gcgagcgcgc agctgcctgc aggggcgcct gatgcggtat tttctcctta cgcatctgtg
     2041 cggtatttca caccgcatac gtcaaagcaa ccatagtacg cgccctgtag cggcgcatta
     2101 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccttagcg
     2161 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa
     2221 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc
     2281 aaaaaacttg atttgggtga tggttcacgt agtgggccat cgccctgata gacggttttt
     2341 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca
     2401 acactcaact ctatctcggg ctattctttt gatttataag ggattttgcc gatttcggtc
     2461 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta
     2521 acgtttacaa ttttatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc
     2581 cagccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca
     2641 tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg
     2701 tcatcaccga aacgcgcgag acgaaagggc ctcgtgatac gcctattttt ataggttaat
     2761 gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga
     2821 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa
     2881 ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt
     2941 gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg
     3001 ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg
     3061 gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg
     3121 agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag
     3181 caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca
     3241 gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg
     3301 agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc
     3361 gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg
     3421 aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg
     3481 ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac
     3541 tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg
     3601 tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg
     3661 gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact
     3721 atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa
     3781 ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt
     3841 aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag
     3901 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
     3961 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
     4021 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
     4081 cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct
     4141 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
     4201 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
     4261 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
     4321 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
     4381 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
     4441 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
     4501 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
     4561 ttacggttcc tggccttttg ctggcctttt gctcacatgt cct
//
