LOCUS       sequence-449668-        9685 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of cell-surface protein extracellular domain 
            fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9685)
  AUTHORS   Wojtowicz WM, Vielmetter J, Fernandes RA, Siepe DH, Eastman CL, 
            Chisholm GB, Cox S, Klock H, Anderson PW, Rue SM, Miller JJ, Glaser 
            SM, Bragstad ML, Vance J, Lam AW, Lesley SA, Zinn K, Garcia KC
  TITLE     A Human IgSF Cell-Surface Interactome Reveals a Complex Network of 
            Protein-Protein Interactions.
  JOURNAL   Cell. 2020 Aug 20;182(4):1027-1043.e17. doi: 
            10.1016/j.cell.2020.07.025.
  PUBMED    32822567
REFERENCE   2  (bases 1 to 9685)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2020.07"; journalName: "Cell"; date: "2020-08-20- 
            20"; volume: "182"; issue: "4"; pages: "1027-1043.e17"
COMMENT     Sequence Label: pD649-HAsp-NFASC-Fc(DAPA)-AviTag-6xHis
FEATURES             Location/Qualifiers
     source          1..9685
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     124..143
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     149..168
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      254..842
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     743..762
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     996..1013
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     1111..1128
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1112..1128
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1584..1600
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     enhancer        1767..2146
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2147..2350
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2301..2321
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2347..2371
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        2581..2599
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     2581..2598
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     2612..2631
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     CDS             7027..7071
                     /codon_start=1
                     /product="peptide tag that allows for enzymatic 
                     biotinylation"
                     /label=AviTag(TM)
                     /translation="GLNDIFEAQKIEWHE"
     CDS             7072..7089
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(7229..7246)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7235..7346
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(7464..7484)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7469..7798
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7649..7784
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7711..7730
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        7846..7893
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             7912..8511
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(7912..7931)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     8408..8428
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     polyA_signal    8641..8762
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8678..8697)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8732..8751
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     CDS             complement(join(8815..9606,9607..9675))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9438..9457
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(join(9676..9685,1..82))
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
       61 tgtatttaga aaaataaaca aataggggtc agtgttacaa ccaattaacc aattctgaac
      121 attatcgcga gcccatttat acctgaatat ggctcataac accccttgct catgaccaaa
      181 atcccttaac gtgagttacg cgcgcgtcgt tccactgagc gtcagacccc gtagaaaaga
      241 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
      301 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
      361 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
      421 tagcccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
      481 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
      541 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
      601 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
      661 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
      721 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
      781 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
      841 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
      901 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
      961 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     1021 aaggcgagag tagggaactg ccaggcatca aactaagcag aaggcccctg acggatggcc
     1081 tttttgcgtt tctacaaact ctttctgtgt tgtaaaacga cggccagtct taagctcggg
     1141 ccccctgggc ggttctgata acgagtaatc gttaatccgc aaataacgta aaaacccgct
     1201 tcggcgggtt tttttatggg gggagtttag ggaaagagca tttgtcagaa tatttaaggg
     1261 cgcctgtcac tttgcttgat atatgagaat tatttaacct tataaatgag aaaaaagcaa
     1321 cgcactttaa ataagatacg ttgctttttc gattgatgaa cacctataat taaactattc
     1381 atctattatt tatgattttt tgtatataca atatttctag tttgttaaag agaattaaga
     1441 aaataaatct cgaaaataat aaagggaaaa tcagtttttg atatcaaaat tatacatgtc
     1501 aacgataata caaaatataa tacaaactat aagatgttat cagtatttat tatcatttag
     1561 aataaatttt gtgtcgccct taattgtgag cggataacaa ttacgagctt catgcacagt
     1621 ggcgttgact caatattggc cattagccat attattcatt ggttatatag cataaatcaa
     1681 tattggctat tggccattgc atacgttgta tctatatcat aatatgtaca tttatattgg
     1741 ctcatgtcca atatgaccgc catgttggca ttgattattg actagttatt aatagtaatc
     1801 aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt
     1861 aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta
     1921 tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg
     1981 gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga
     2041 cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt
     2101 tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg
     2161 gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc
     2221 cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg
     2281 taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat
     2341 aagcagagct cgtttagtga accgtcagat cgcctggaga cgccatccac gctgttttga
     2401 cctccataga agacaccggg accgatccag cctccgcggc cgggaacggt gcattggaac
     2461 gcggattccc cgtgccaaga gtgacgtaag taccgcctat agagtctata ggcccacccc
     2521 cttggcttcg ttagaacgcg gctacaatta atacataacc ttatgtatca tacacatacg
     2581 atttaggtga cactatagaa taacatccac tttgcctttc tctccacagg tgtccactcc
     2641 caggtccaac tgcacctcgg ttctatcgaa aacgcgcctg ctagcgccac catgaagacc
     2701 atcattgccc tgagctacat cttctgcctg gtgttcgctg cggccgccat tgagatccct
     2761 atggacccct ctatccagaa cgagctgacc cagcctccta ccatcacaaa gcagagcgcc
     2821 aaggaccaca tcgtggaccc cagagacaac atcctgatcg agtgcgaggc caagggcaac
     2881 cctgctccta gcttccactg gacccggaac agccggttct tcaatatcgc caaagatccc
     2941 cgggtgtcca tgagaaggcg ctctggcaca ctggtcatcg actttagaag cggcggcaga
     3001 cctgaggaat acgagggcga gtaccagtgc ttcgcccgga acaaatttgg cacagccctg
     3061 agcaaccgga tcagactcca ggtgtccaag tctcccctgt ggcctaaaga aaatctggac
     3121 cccgtggtgg tgcaagaggg tgctcctctg actctgcagt gcaatcctcc tcctggactg
     3181 ccctctcctg tgatcttttg gatgagcagc agcatggaac ccatcacaca ggacaagaga
     3241 gtgtcccagg gccacaacgg cgacctgtac ttcagcaacg tgatgctgca ggacatgcag
     3301 accgactaca gctgcaacgc cagattccac ttcacccaca ccatccagca gaagaacccc
     3361 ttcacactga aggtgctgac cacaagaggc gtggccgaga gaacccctag cttcatgtat
     3421 cctcagggca ccgccagcag ccagatggtg cttagaggaa tggacctgct gctggaatgt
     3481 atcgccagcg gcgtgccaac acctgatatc gcctggtaca agaaaggcgg cgatctgccc
     3541 agcgacaagg ccaagttcga gaacttcaac aaggccctgc ggatcaccaa cgtgtccgag
     3601 gaagatagcg gcgagtactt ctgcctggcc agcaacaaga tgggcagcat cagacacacc
     3661 atcagcgtca gagtgaaggc cgctccttac tggctggacg agcccaagaa tctgattctg
     3721 gcccctggcg aagatggcag actcgtgtgt agagccaacg gcaatcctaa gcctaccgtg
     3781 cagtggatgg tcaacggcga acctctgcag tctgcccctc ctaatcctaa tagagaggtg
     3841 gccggcgaca ccatcatctt cagggatacc cagatcagca gcagagccgt gtaccagtgt
     3901 aacaccagca acgagcacgg ctacctgctg gccaatgctt tcgtgtctgt gctggacgtg
     3961 ccacctagaa tgctgagccc cagaaaccag ctgatccgcg tgatcctgta caacagaacc
     4021 cggctggatt gcccattctt cggcagcccc attcctacac tgcggtggtt taagaacggc
     4081 cagggctcta acctggacgg cggcaattac cacgtgtacg agaacggcag cctggaaatc
     4141 aagatgatcc ggaaagagga ccagggcatc tacacatgcg tggccaccaa tatcctgggc
     4201 aaagccgaga atcaagtgcg gctggaagtg aaggacccca ccagaatcta cagaatgccc
     4261 gaggatcagg tggccagacg gggaacaaca gtgcagctgg aatgcagagt gaaacacgac
     4321 cccagcctga agctgaccgt gtcctggctg aaggacgacg agcctctgta catcggcaac
     4381 aggatgaaga aagaggatga cagcctgacc atcttcggag tggccgaaag agatcagggc
     4441 tcctatacct gtgtggccag caccgaactg gaccaggatc tggccaaagc ctatctgaca
     4501 gtgctggccg atcaggccac acctacaaat agactggccg ctctgcctaa gggcagaccc
     4561 gatagaccta gagatctgga actgaccgac ctggctgaga gatctgtgcg gcttacatgg
     4621 attcccggcg acgccaacaa cagccccatc acagattacg tggtgcagtt cgaagaggat
     4681 cagtttcagc ccggcgtgtg gcacgatcac agcaagtatc ctggctccgt gaacagcgcc
     4741 gtgctgagac tgagccctta cgtgaactac cagttcagag tgatcgccat caatgaagtg
     4801 ggcagctctc accctagcct gcctagcgag agatacagaa caagcggagc ccctcctgag
     4861 agcaaccctg gggatgtgaa aggcgagggc accagaaaga acaacatgga aatcacctgg
     4921 acacccatga acgccaccag cgcctttgga cccaacctgc ggtatattgt gaagtggcgg
     4981 cggagagaga cacgcgaggc ttggaacaat gtgaccgtgt ggggcagcag atacgtcgtg
     5041 ggacagacac ctgtgtacgt gccctacgag attagagtgc aggccgagaa cgatttcggc
     5101 aagggacctg agcctgagag cgtgatcgga tacagcggcg aggattatcc tagagccgct
     5161 cctaccgaag tgaaagtgcg cgtgatgaac agcacagcca tcagcctgca gtggaacaga
     5221 gtgtacagcg acacagtgca gggccagctg agagagtacc gggcctacta ttggagagag
     5281 agcagcctgc tgaagaacct gtgggtgtcc cagaagagac agcaggccag ctttcctggc
     5341 gatagactga gaggcgtcgt gtccagactg ttcccctact ccaactacaa gctggaaatg
     5401 gtggtcgtga acggcagagg cgacggccct agaagcgaga caaaagagtt taccacacct
     5461 gagggcgtgc ccagcgctcc tagaaggttt agagtgcggc agcccaacct ggaaaccatc
     5521 aatctggaat gggatcaccc tgagcacccc aacggcatca tgatcggcta caccctgaaa
     5581 tacgtggcct tcaacggcac caaagtgggc aagcagatcg tggaaaactt cagccccaac
     5641 cagaccaagt tcaccgtgca gagaaccgat ccagtgtcca gataccggtt caccctgtcc
     5701 gccagaacac aagttggatc tggcgaggcc gtgaccgagg aatctcctgc tcctccaaat
     5761 gaggccactc caacagctgc tccaccaaca ctgccaccta caacagttgg agccacaggc
     5821 gccgtgtcta gcacagatgc tacagccatt gccgccacca cagaggccac aaccgtgcct
     5881 attattccca cagtggcccc taccacaatc gccacaacaa ccaccgtggc taccaccaca
     5941 actaccacag ccgctgccac aaccacaaca gagagccctc caacaacaac ctccggcacc
     6001 aagatccacg agagcgcccc tgatgagcag tccatttgga atgtgactgt gctgcctaac
     6061 agcaagtggg ccaacatcac atggaagcac aacttcggcc ctggcaccga cttcgtggtc
     6121 gagtacatcg acagcaacca caccaaaaag accgtgcctg tgaaggccca ggctcagcct
     6181 attcagctga ccgatctgta ccccggcatg acctacacac tgcgggtgta cagcagagac
     6241 aatgagggca tcagctccac cgtgatcacc ttcatgacca gcaccgccta caccaacaac
     6301 caggccgata ttgccacaca aggctggggc gcgccgggcg gcgacaagac ccacacctgc
     6361 cccccctgcc cagctccaga actgctggga gggccttccg tgttcctgtt cccccccaag
     6421 cccaaggaca ccctgatgat cagcaggacc cccgaggtga cctgcgtggt ggtggccgtg
     6481 tcccacgagg acccagaggt gaagttcaac tggtacgtgg acggcgtgga ggtgcacaac
     6541 gccaagacca agcccagaga ggagcagtac aacagcacct acagggtggt gtccgtgctg
     6601 accgtgctgc accaggactg gctgaacggc aaagaataca agtgcaaagt ctccaacaag
     6661 gccctggctg ccccaatcga aaagacaatc agcaaggcca agggccagcc acgggagccc
     6721 caggtgtaca ccctgccccc cagccgggag gagatgacca agaaccaggt gtccctgacc
     6781 tgtctggtga agggcttcta ccccagcgat atcgccgtgg agtgggagag caacggccag
     6841 cccgagaaca actacaagac caccccccca gtgctggaca gcgacggcag cttcttcctg
     6901 tacagcaagc tgaccgtgga caagtccagg tggcagcagg gcaacgtgtt cagctgcagc
     6961 gtgatgcacg aggccctgca caaccactac acccagaagt ccctgagcct gagccccggc
     7021 ggcagcggtc ttaatgatat tttcgaggct cagaagattg agtggcatga acaccaccac
     7081 catcaccact aaggccggcc tgtagtcact ggcacaacgc gttacccggt aagccaatcg
     7141 ggtatacacg gtcgtcatac tgcagacagg gttcttctac tttgcaagat agtcttgagt
     7201 agtaaaataa atagatagag aaaatcagcc tcgactgtgc cttctagttg ccagccatct
     7261 gttgtttgcc cctcccccgt gccttccttg accctggaag gtgccactcc cactgtcctt
     7321 tcctaataaa atgaggaaat tgcatcacaa cactcaaccc tatctcggtc tattcttttg
     7381 atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa
     7441 aatttaacgc gaattaattc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg
     7501 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg
     7561 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc
     7621 aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca
     7681 ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg ccgcctctgc
     7741 ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa
     7801 gctcccggga gcttgtatat ccattttcgg atctgatcag cacgtgttga caattaatca
     7861 tcggcatagt atatcggcat agtataatac gacaaggtga ggaactaaac catgaccgag
     7921 tacaagccca cggtgcgcct cgccacccgc gacgacgtcc ccagggccgt acgcaccctc
     7981 gccgccgcgt tcgccgacta ccccgccacg cgccacaccg tcgatccgga ccgccacatc
     8041 gagcgggtca ccgagctgca agaactcttc ctcacgcgcg tcgggctcga catcggcaag
     8101 gtgtgggtcg cggacgacgg cgccgcggtg gcggtctgga ccacgccgga gagcgtcgaa
     8161 gcgggggcgg tgttcgccga gatcggcccg cgcatggccg agttgagcgg ttcccggctg
     8221 gccgcgcagc aacagatgga aggcctcctg gcgccgcacc ggcccaagga gcccgcgtgg
     8281 ttcctggcca ccgtcggcgt ctcgcccgac caccagggca agggtctggg cagcgccgtc
     8341 gtgctccccg gagtggaggc ggccgagcgc gccggggtgc ccgccttcct ggagacatcc
     8401 gcgccccgca acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc cgacgtcgag
     8461 gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc ccggtgcctg acacgtgcta
     8521 cgagatttcg attccaccgc cgccttctat gaaaggttgg gcttcggaat cgttttccgg
     8581 gacgccggct ggatgatcct ccagcgcggg gatctcatgc tggagttctt cgcccacccc
     8641 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca
     8701 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct
     8761 tatcatgtct gtataccgtc gacctctagc tagagcttgg cgtaatcatg gtcattacca
     8821 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
     8881 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagcgc
     8941 tgcgatgata ccgcgagaac cacgctcacc ggctccggat ttatcagcaa taaaccagcc
     9001 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
     9061 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
     9121 tgccatcgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc
     9181 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag
     9241 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt
     9301 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
     9361 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
     9421 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat
     9481 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
     9541 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
     9601 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
     9661 atgttgaata ctcatattct tcctt
//
