LOCUS       sequence-449681-        8266 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  1) Ectopic expression of MAAP protein variant in mammalian cells. 2)
            Packaging MAAP variant sequence into lentivirus for stable 
            expression in mammalian cells. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8266)
  AUTHORS   Schieferecke AJ, Lee H, Chen A, Kilaru V, Krish Williams J, Schaffer
            DV
  TITLE     Evolving membrane-associated accessory protein variants for improved
            adeno-associated virus production.
  JOURNAL   Mol Ther. 2024 Feb 7;32(2):340-351. doi: 
            10.1016/j.ymthe.2023.12.015. Epub 2023 Dec 18.
  PUBMED    38115579
REFERENCE   2  (bases 1 to 8266)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ymthe.2023.12.015"; journalName: "Mol Ther"; date: 
            "2024-02-7- 7"; volume: "32"; issue: "2"; pages: "340-351"
COMMENT     Sequence Label: pCW57-CMV-MAAP-SL01V9
FEATURES             Location/Qualifiers
     source          1..8266
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    41..158
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        212..591
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        592..795
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     746..766
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     840..859
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        840..858
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      884..893
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        1208..1718
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     1637..1655
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             1728..2324
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(1728..1747)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2224..2244
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             2325..2378
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             join(2379..2996,2997..3002,3003..3038,3039..3041,3042..3077,
                     3078..3080,3081..3116,3117..3125)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     primer_bind     complement(3165..3185)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3583..3594)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3799..4032
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4104..4225
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4141..4160)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4195..4214
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4265..4400
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        4400..4471
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4472..4540,4541..5332)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4690..4709)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5503..6091
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5992..6011
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6245..6262
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6379..6400
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6415..6420,6421..6438,6439..6445)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6453..6469
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6458..6480
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6477..6493
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6477..6493
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6512..6532
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6514..6532
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6560..6786
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             6787..6967
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7014..7139
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7632..7865
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8050..8094
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 aattctcgac ctcgagacaa atggcagtat tcatccacaa ttttaaaaga aaagggggga
       61 ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac atacaaacta
      121 aagaattaca aaaacaaatt acaaaaattc aaaattttcg ggtttattac agggacagca
      181 gagatccact ttggccgcga atcgatgcgt tgacattgat tattgactag ttattaatag
      241 taatcaatta cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt
      301 acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg
      361 acgtatgttc ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat
      421 ttacggtaaa ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct
      481 attgacgtca atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg
      541 gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg
      601 ttttggcagt acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc
      661 caccccattg acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa
      721 tgtcgtaaca actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc
      781 tatataagca gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt
      841 aatacgactc actataggga gacccaagct tggtaccacc ggtgccacca tggagcccct
      901 caacccaagg caaatcaaca acatcaagac aacgctcgag gtcttgtgct tccgggttac
      961 aaataccttg gacccggcaa cggactcgac aagggggagc cggtcaacgc agcagacgcg
     1021 gcggccctcg agcacgacaa ggcctacgac cagcagctca aggccggaga caacccgtac
     1081 ctcaagtaca accacgccga cgccgagttc caggagcggc tcaaagaaga tacgtctttt
     1141 gggggcaacc tcgggcgaaa aagaaggcag ctaataacct aggctgcaga cgcgttgtac
     1201 aggatccggg gttggggttg cgccttttcc aaggcagccc tgggtttgcg cagggacgcg
     1261 gctgctctgg gcgtggttcc gggaaacgca gcggcgccga ccctgggtct cgcacattct
     1321 tcacgtccgt tcgcagcgtc acccggatct tcgccgctac ccttgtgggc cccccggcga
     1381 cgcttcctgc tccgccccta agtcgggaag gttccttgcg gttcgcggcg tgccggacgt
     1441 gacaaacgga agccgcacgt ctcactagta ccctcgcaga cggacagcgc cagggagcaa
     1501 tggcagcgcg ccgaccgcga tgggctgtgg ccaatagcgg ctgctcagca gggcgcgccg
     1561 agagcagcgg ccgggaaggg gcggtgcggg aggcggggtg tggggcggta gtgtgggccc
     1621 tgttcctgcc cgcgcggtgt tccgcattct gcaagcctcc ggagcgcacg tcggcagtcg
     1681 gctccctcgt tgaccgaatc accgacctct ctccccagca attcaccatg accgagtaca
     1741 agcccacggt gcgcctcgcc acccgcgacg acgtccccag ggccgtacgc accctcgccg
     1801 ccgcgttcgc cgactacccc gccacgcgcc acaccgtcga tccggaccgc cacatcgagc
     1861 gggtcaccga gctgcaagaa ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt
     1921 gggtcgcgga cgacggcgcc gcggtggcgg tctggaccac gccggagagc gtcgaagcgg
     1981 gggcggtgtt cgccgagatc ggcccgcgca tggccgagtt gagcggttcc cggctggccg
     2041 cgcagcaaca gatggaaggc ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc
     2101 tggccaccgt cggcgtctcg cccgaccacc agggcaaggg tctgggcagc gccgtcgtgc
     2161 tccccggagt ggaggcggcc gagcgcgccg gggtgcccgc cttcctggag acctccgcgc
     2221 cccgcaacct ccccttctac gagcggctcg gcttcaccgt caccgccgac gtcgaggtgc
     2281 ccgaaggacc gcgcacctgg tgcatgaccc gcaagcccgg tgccgaaggt agaggttctc
     2341 tcctcacttg tggtgatgtt gaagaaaacc ctggtccaat gtctagactg gacaagagca
     2401 aagtcataaa cggagctctg gaattactca atggtgtcgg tatcgaaggc ctgacgacaa
     2461 ggaaactcgc tcaaaagctg ggagttgagc agcctaccct gtactggcac gtgaagaaca
     2521 agcgggccct gctcgatgcc ctgccaatcg agatgctgga caggcatcat acccacttct
     2581 gccccctgga aggcgagtca tggcaagact ttctgcggaa caacgccaag tcataccgct
     2641 gtgctctcct ctcacatcgc gacggggcta aagtgcatct cggcacccgc ccaacagaga
     2701 aacagtacga aaccctggaa aatcagctcg cgttcctgtg tcagcaaggc ttctccctgg
     2761 agaacgcact gtacgctctg tccgccgtgg gccactttac actgggctgc gtattggagg
     2821 aacaggagca tcaagtagca aaagaggaaa gagagacacc taccaccgat tctatgcccc
     2881 cacttctgag acaagcaatt gagctgttcg accggcaggg agccgaacct gccttccttt
     2941 tcggcctgga actaatcata tgtggcctgg agaaacagct aaagtgcgaa agcggcgggc
     3001 cgaccgacgc ccttgacgat tttgacttag acatgctccc agccgatgcc cttgacgact
     3061 ttgaccttga tatgctgcct gctgacgctc ttgacgattt tgaccttgac atgctccccg
     3121 ggtaactaag taaggatcga tccaagatat cgtattctta actatgttgc tccttttacg
     3181 ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc
     3241 attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt
     3301 gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc
     3361 attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg
     3421 gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact
     3481 gacaattccg tggtgttgtc ggggaagctg acgtcctttc catggctgct cgcctgtgtt
     3541 gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg
     3601 gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc
     3661 cctcagacga gtcggatctc cctttgggcc gcctccccgc ctgtttcgcc tcggcgtccg
     3721 gactagaggt acctttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt
     3781 taaaagaaaa ggggggactg gaagggctaa ttcactccca acgaagacaa gatctgcttt
     3841 ttgcttgtac tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac
     3901 tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg
     3961 cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga
     4021 aaatctctag cagtagtagt tcatgtcatc ttattattca gtatttataa cttgcaaaga
     4081 aatgaatatc agagagtgag aggaacttgt ttattgcagc ttataatggt tacaaataaa
     4141 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt
     4201 tgtccaaact catcaatgta tcttatcatg tctggctcta gctatcccgc ccctaactcc
     4261 gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat
     4321 tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg
     4381 aggaggcttt tttggaggcc tttcaaatat gtatccgctc atgagacaat aaccctgata
     4441 aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct
     4501 tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa
     4561 agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa
     4621 cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt
     4681 taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg
     4741 tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca
     4801 tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa
     4861 cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt
     4921 gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc
     4981 cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa
     5041 actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga
     5101 ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc
     5161 tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga
     5221 tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga
     5281 acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga
     5341 ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat
     5401 ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt
     5461 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
     5521 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
     5581 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
     5641 aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
     5701 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
     5761 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
     5821 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
     5881 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
     5941 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
     6001 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
     6061 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt
     6121 cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt
     6181 ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga
     6241 gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc
     6301 cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg
     6361 cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca
     6421 ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat ttcacacagg
     6481 aaacagctat gaccatgatt acgccaagcg cgcaattaac cctcactaaa gggaacaaaa
     6541 gctggagctg caagcttaat gtagtcttat gcaatactct tgtagtcttg caacatggta
     6601 acgatgagtt agcaacatgc cttacaagga gagaaaaagc accgtgcatg ccgattggtg
     6661 gaagtaaggt ggtacgatcg tgccttatta ggaaggcaac agacgggtct gacatggatt
     6721 ggacgaacca ctgaattgcc gcattgcaga gatattgtat ttaagtgcct agctcgatac
     6781 ataaacgggt ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg
     6841 aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt
     6901 ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc
     6961 tctagcagtg gcgcccgaac agggacttga aagcgaaagg gaaaccagag gagctctctc
     7021 gacgcaggac tcggcttgct gaagcgcgca cggcaagagg cgaggggcgg cgactggtga
     7081 gtacgccaaa aattttgact agcggaggct agaaggagag agatgggtgc gagagcgtca
     7141 gtattaagcg ggggagaatt agatcgcgat gggaaaaaat tcggttaagg ccagggggaa
     7201 agaaaaaata taaattaaaa catatagtat gggcaagcag ggagctagaa cgattcgcag
     7261 ttaatcctgg cctgttagaa acatcagaag gctgtagaca aatactggga cagctacaac
     7321 catcccttca gacaggatca gaagaactta gatcattata taatacagta gcaaccctct
     7381 attgtgtgca tcaaaggata gagataaaag acaccaagga agctttagac aagatagagg
     7441 aagagcaaaa caaaagtaag accaccgcac agcaagcggc cgctgatctt cagacctgga
     7501 ggaggagata tgagggacaa ttggagaagt gaattatata aatataaagt agtaaaaatt
     7561 gaaccattag gagtagcacc caccaaggca aagagaagag tggtgcagag agaaaaaaga
     7621 gcagtgggaa taggagcttt gttccttggg ttcttgggag cagcaggaag cactatgggc
     7681 gcagcgtcaa tgacgctgac ggtacaggcc agacaattat tgtctggtat agtgcagcag
     7741 cagaacaatt tgctgagggc tattgaggcg caacagcatc tgttgcaact cacagtctgg
     7801 ggcatcaagc agctccaggc aagaatcctg gctgtggaaa gatacctaaa ggatcaacag
     7861 ctcctgggga tttggggttg ctctggaaaa ctcatttgca ccactgctgt gccttggaat
     7921 gctagttgga gtaataaatc tctggaacag atttggaatc acacgacctg gatggagtgg
     7981 gacagagaaa ttaacaatta cacaagctta atacactcct taattgaaga atcgcaaaac
     8041 cagcaagaaa agaatgaaca agaattattg gaattagata aatgggcaag tttgtggaat
     8101 tggtttaaca taacaaattg gctgtggtat ataaaattat tcataatgat agtaggaggc
     8161 ttggtaggtt taagaatagt ttttgctgta ctttctatag tgaatagagt taggcaggga
     8221 tattcaccat tatcgtttca gacccacctc ccaaccccga ggggac
//
