LOCUS       sequence-449683-        9232 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  1) Ectopic expression of MAAP protein variant in mammalian cells. 2)
            Packaging MAAP variant sequence into lentivirus for stable 
            expression in mammalian cells. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9232)
  AUTHORS   Schieferecke AJ, Lee H, Chen A, Kilaru V, Krish Williams J, Schaffer
            DV
  TITLE     Evolving membrane-associated accessory protein variants for improved
            adeno-associated virus production.
  JOURNAL   Mol Ther. 2024 Feb 7;32(2):340-351. doi: 
            10.1016/j.ymthe.2023.12.015. Epub 2023 Dec 18.
  PUBMED    38115579
REFERENCE   2  (bases 1 to 9232)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ymthe.2023.12.015"; journalName: "Mol Ther"; date: 
            "2024-02-7- 7"; volume: "32"; issue: "2"; pages: "340-351"
COMMENT     Sequence Label: pCW57-CMV-MAAP-SL08V9
FEATURES             Location/Qualifiers
     source          1..9232
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    44..161
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(374..418)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    603..836
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    1329..1454
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             1501..1681
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(1682..1908)
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     primer_bind     complement(1936..1956)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(1936..1954)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(1975..1991)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1975..1991)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1988..2010)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1999..2015
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2023..2029,2030..2047,2048..2053))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2068..2089
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2206..2223)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2377..2965)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2457..2476)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3136..3927,3928..3996))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3759..3778
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3997..4068)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      4068..4203
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    4243..4364
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4254..4273)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4308..4327
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     LTR             4436..4669
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     CDS             4874..4885
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     5283..5303
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(join(5343..5351,5352..5387,5388..5390,5391..5426,
                     5427..5429,5430..5465,5466..5471,5472..6089))
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     CDS             complement(6090..6143)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             complement(6144..6740)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6224..6244)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     6721..6740
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     promoter        complement(6750..7260)
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     complement(6813..6831)
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     regulatory      8541..8550
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(8575..8594)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8576..8594)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(8639..8842)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(8668..8688)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        8843..9222
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 ttcgcggcca aagtggatct ctgctgtccc tgtaataaac ccgaaaattt tgaatttttg
       61 taatttgttt ttgtaattct ttagtttgta tgtctgttgc tattatgtct actattcttt
      121 cccctgcact gtacccccca atcccccctt ttcttttaaa attgtggatg aatactgcca
      181 tttgtctcga ggtcgagaat tgtcccctcg gggttgggag gtgggtctga aacgataatg
      241 gtgaatatcc ctgcctaact ctattcacta tagaaagtac agcaaaaact attcttaaac
      301 ctaccaagcc tcctactatc attatgaata attttatata ccacagccaa tttgttatgt
      361 taaaccaatt ccacaaactt gcccatttat ctaattccaa taattcttgt tcattctttt
      421 cttgctggtt ttgcgattct tcaattaagg agtgtattaa gcttgtgtaa ttgttaattt
      481 ctctgtccca ctccatccag gtcgtgtgat tccaaatctg ttccagagat ttattactcc
      541 aactagcatt ccaaggcaca gcagtggtgc aaatgagttt tccagagcaa ccccaaatcc
      601 ccaggagctg ttgatccttt aggtatcttt ccacagccag gattcttgcc tggagctgct
      661 tgatgcccca gactgtgagt tgcaacagat gctgttgcgc ctcaatagcc ctcagcaaat
      721 tgttctgctg ctgcactata ccagacaata attgtctggc ctgtaccgtc agcgtcattg
      781 acgctgcgcc catagtgctt cctgctgctc ccaagaaccc aaggaacaaa gctcctattc
      841 ccactgctct tttttctctc tgcaccactc ttctctttgc cttggtgggt gctactccta
      901 atggttcaat ttttactact ttatatttat ataattcact tctccaattg tccctcatat
      961 ctcctcctcc aggtctgaag atcagcggcc gcttgctgtg cggtggtctt acttttgttt
     1021 tgctcttcct ctatcttgtc taaagcttcc ttggtgtctt ttatctctat cctttgatgc
     1081 acacaataga gggttgctac tgtattatat aatgatctaa gttcttctga tcctgtctga
     1141 agggatggtt gtagctgtcc cagtatttgt ctacagcctt ctgatgtttc taacaggcca
     1201 ggattaactg cgaatcgttc tagctccctg cttgcccata ctatatgttt taatttatat
     1261 tttttctttc cccctggcct taaccgaatt ttttcccatc gcgatctaat tctcccccgc
     1321 ttaatactga cgctctcgca cccatctctc tccttctagc ctccgctagt caaaattttt
     1381 ggcgtactca ccagtcgccg cccctcgcct cttgccgtgc gcgcttcagc aagccgagtc
     1441 ctgcgtcgag agagctcctc tggtttccct ttcgctttca agtccctgtt cgggcgccac
     1501 tgctagagat tttccacact gactaaaagg gtctgaggga tctctagtta ccagagtcac
     1561 acaacagacg ggcacacact acttgaagca ctcaaggcaa gctttattga ggcttaagca
     1621 gtgggttccc tagttagcca gagagctccc aggctcagat ctggtctaac cagagagacc
     1681 cgtttatgta tcgagctagg cacttaaata caatatctct gcaatgcggc aattcagtgg
     1741 ttcgtccaat ccatgtcaga cccgtctgtt gccttcctaa taaggcacga tcgtaccacc
     1801 ttacttccac caatcggcat gcacggtgct ttttctctcc ttgtaaggca tgttgctaac
     1861 tcatcgttac catgttgcaa gactacaaga gtattgcata agactacatt aagcttgcag
     1921 ctccagcttt tgttcccttt agtgagggtt aattgcgcgc ttggcgtaat catggtcata
     1981 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag
     2041 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg
     2101 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca
     2161 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc
     2221 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
     2281 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
     2341 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
     2401 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
     2461 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
     2521 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
     2581 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
     2641 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
     2701 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
     2761 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac
     2821 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
     2881 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
     2941 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
     3001 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
     3061 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
     3121 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
     3181 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
     3241 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
     3301 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
     3361 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
     3421 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt
     3481 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
     3541 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
     3601 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
     3661 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
     3721 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
     3781 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
     3841 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
     3901 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
     3961 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
     4021 aagcatttat cagggttatt gtctcatgag cggatacata tttgaaaggc ctccaaaaaa
     4081 gcctcctcac tacttctgga atagctcaga ggccgaggcg gcctcggcct ctgcataaat
     4141 aaaaaaaatt agtcagccat ggggcggaga atgggcggaa ctgggcggag ttaggggcgg
     4201 gatgggcgga gttaggggcg ggatagctag agccagacat gataagatac attgatgagt
     4261 ttggacaaac cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg
     4321 ctattgcttt atttgtaacc attataagct gcaataaaca agttcctctc actctctgat
     4381 attcatttct ttgcaagtta taaatactga ataataagat gacatgaact actactgcta
     4441 gagattttcc acactgacta aaagggtctg agggatctct agttaccaga gtcacacaac
     4501 agacgggcac acactacttg aagcactcaa ggcaagcttt attgaggctt aagcagtggg
     4561 ttccctagtt agccagagag ctcccaggct cagatctggt ctaaccagag agacccagta
     4621 caagcaaaaa gcagatcttg tcttcgttgg gagtgaatta gcccttccag tccccccttt
     4681 tcttttaaaa agtggctaag atctacagct gccttgtaag tcattggtct taaaggtacc
     4741 tctagtccgg acgccgaggc gaaacaggcg gggaggcggc ccaaagggag atccgactcg
     4801 tctgagggcg aaggcgaaga cgcggaagag gccgcagagc cggcagcagg ccgcgggaag
     4861 gaaggtccgc tggattgagg gccgaaggga cgtagcagaa ggacgtcccg cgcagaatcc
     4921 aggtggcaac acaggcgagc agccatggaa aggacgtcag cttccccgac aacaccacgg
     4981 aattgtcagt gcccaacagc cgagcccctg tccagcagcg ggcaaggcag gcggcgatga
     5041 gttccgccgt ggcaataggg agggggaaag cgaaagtccc ggaaaggagc tgacaggtgg
     5101 tggcaatgcc ccaaccagtg ggggttgcgt cagcaaacac agtgcacacc acgccacgtt
     5161 gcctgacaac gggccacaac tcctcataaa gagacagcaa ccaggattta tacaaggagg
     5221 agaaaatgaa agccatacgg gaagcaatag catgatacaa aggcattaaa gcagcgtatc
     5281 cacatagcgt aaaaggagca acatagttaa gaatacgata tcttggatcg atccttactt
     5341 agttacccgg ggagcatgtc aaggtcaaaa tcgtcaagag cgtcagcagg cagcatatca
     5401 aggtcaaagt cgtcaagggc atcggctggg agcatgtcta agtcaaaatc gtcaagggcg
     5461 tcggtcggcc cgccgctttc gcactttagc tgtttctcca ggccacatat gattagttcc
     5521 aggccgaaaa ggaaggcagg ttcggctccc tgccggtcga acagctcaat tgcttgtctc
     5581 agaagtgggg gcatagaatc ggtggtaggt gtctctcttt cctcttttgc tacttgatgc
     5641 tcctgttcct ccaatacgca gcccagtgta aagtggccca cggcggacag agcgtacagt
     5701 gcgttctcca gggagaagcc ttgctgacac aggaacgcga gctgattttc cagggtttcg
     5761 tactgtttct ctgttgggcg ggtgccgaga tgcactttag ccccgtcgcg atgtgagagg
     5821 agagcacagc ggtatgactt ggcgttgttc cgcagaaagt cttgccatga ctcgccttcc
     5881 agggggcaga agtgggtatg atgcctgtcc agcatctcga ttggcagggc atcgagcagg
     5941 gcccgcttgt tcttcacgtg ccagtacagg gtaggctgct caactcccag cttttgagcg
     6001 agtttccttg tcgtcaggcc ttcgataccg acaccattga gtaattccag agctccgttt
     6061 atgactttgc tcttgtccag tctagacatt ggaccagggt tttcttcaac atcaccacaa
     6121 gtgaggagag aacctctacc ttcggcaccg ggcttgcggg tcatgcacca ggtgcgcggt
     6181 ccttcgggca cctcgacgtc ggcggtgacg gtgaagccga gccgctcgta gaaggggagg
     6241 ttgcggggcg cggaggtctc caggaaggcg ggcaccccgg cgcgctcggc cgcctccact
     6301 ccggggagca cgacggcgct gcccagaccc ttgccctggt ggtcgggcga gacgccgacg
     6361 gtggccagga accacgcggg ctccttgggc cggtgcggcg ccaggaggcc ttccatctgt
     6421 tgctgcgcgg ccagccggga accgctcaac tcggccatgc gcgggccgat ctcggcgaac
     6481 accgcccccg cttcgacgct ctccggcgtg gtccagaccg ccaccgcggc gccgtcgtcc
     6541 gcgacccaca ccttgccgat gtcgagcccg acgcgcgtga ggaagagttc ttgcagctcg
     6601 gtgacccgct cgatgtggcg gtccggatcg acggtgtggc gcgtggcggg gtagtcggcg
     6661 aacgcggcgg cgagggtgcg tacggccctg gggacgtcgt cgcgggtggc gaggcgcacc
     6721 gtgggcttgt actcggtcat ggtgaattgc tggggagaga ggtcggtgat tcggtcaacg
     6781 agggagccga ctgccgacgt gcgctccgga ggcttgcaga atgcggaaca ccgcgcgggc
     6841 aggaacaggg cccacactac cgccccacac cccgcctccc gcaccgcccc ttcccggccg
     6901 ctgctctcgg cgcgccctgc tgagcagccg ctattggcca cagcccatcg cggtcggcgc
     6961 gctgccattg ctccctggcg ctgtccgtct gcgagggtac tagtgagacg tgcggcttcc
     7021 gtttgtcacg tccggcacgc cgcgaaccgc aaggaacctt cccgacttag gggcggagca
     7081 ggaagcgtcg ccggggggcc cacaagggta gcggcgaaga tccgggtgac gctgcgaacg
     7141 gacgtgaaga atgtgcgaga cccagggtcg gcgccgctgc gtttcccgga accacgccca
     7201 gagcagccgc gtccctgcgc aaacccaggg ctgccttgga aaaggcgcaa ccccaacccc
     7261 ggatcctgta caacgcgtct gcagcctagg ttacaagtat tggtcgatga gtggattcat
     7321 tagtcggtcc aggctttggc tgtgagcgta gctgctatgg aaaggtacgt tctcaaactc
     7381 gtagctgaac tggaagttgt tacccgttct tagcatttgc gacgggaaat attccaggca
     7441 gtaaaaggac gaacgaccca cggcctggct tccatcatta agcgtcagat acccgtactg
     7501 aggaatcatg aaaacgtccg ctgggaacgg cgggaggcag ccctcgtgag ccgacccgag
     7561 cacgtacggg agctgatagt ctgagtccgt gaagacctgg accgtgctgg taaggttatt
     7621 ggcgatggtc ttgactccat tgttgtccgt aacctctttg acctgaatgt tgaagagctt
     7681 gaagttgagt cgcttaggcc ggaatcccca gttgttgttg atgagtcgct gccagtcacg
     7741 tggtgagaag tggcagtgga atctgttgaa gtcaaaatac ccccaggggg tgctgtagcc
     7801 gaagtaggcg ttgtcatttg aagatcctcc agatgtgctg ttggagattt gcttgtagag
     7861 gtgattgttg taggtgggca gggcccaggt tcgggtgctg gtggtgatga ctctgtcccc
     7921 cagccattgg gaatcgcaat gccaatttcc cgaggaacta cccactccat cggcaccttc
     7981 gttattgtct gccactggtg cgccaccacc tgaagccatt gtaagagatc ccacacctga
     8041 gggggctgcg ggaggttctc cgattggttg agggtctggg actgactctg tgtcgccagt
     8101 ctgaccgaaa ttgagtctct ttttagcggg ctgtgcaccc gatttgccaa tacccgcgga
     8161 ggagtccggt tcctgaggag actgctctac aggcctcttc tttccaggag ccgtcttagc
     8221 cgcttcctca accagaccaa gaggcaagaa gcctcttttt ggcctggaag actgctcgcc
     8281 cgaggttgcc cccaaaagac gtatcttctt tgagccgctc ctggaactcg gcgtcggcgt
     8341 ggttgtactt gaggtacggg ttgtctccgg ccttgagctg ctggtcgtag gccttgtcgt
     8401 gctcgagggc cgccgcgtct gctgcgttga ccggctcccc cttgtcgagt ccgttgccgg
     8461 gtccaaggta tttgtaaccc ggaagcacaa gacctcgagc gttgtcttga tgttgttgat
     8521 ttgccttggg ttgaggggct ccatggtggc accggtggta ccaagcttgg gtctccctat
     8581 agtgagtcgt attaatttcg ataagccagt aagcagtggg ttctctagtt agccagagag
     8641 ctctgcttat atagacctcc caccgtacac gcctaccgcc catttgcgtc aatggggcgg
     8701 agttgttacg acattttgga aagtcccgtt gattttggtg ccaaaacaaa ctcccattga
     8761 cgtcaatggg gtggagactt ggaaatcccc gtgagtcaaa ccgctatcca cgcccattga
     8821 tgtactgcca aaaccgcatc accatggtaa tagcgatgac taatacgtag atgtactgcc
     8881 aagtaggaaa gtcccataag gtcatgtact gggcataatg ccaggcgggc catttaccgt
     8941 cattgacgtc aatagggggc gtacttggca tatgatacac ttgatgtact gccaagtggg
     9001 cagtttaccg taaatactcc acccattgac gtcaatggaa agtccctatt ggcgttacta
     9061 tgggaacata cgtcattatt gacgtcaatg ggcgggggtc gttgggcggt cagccaggcg
     9121 ggccatttac cgtaagttat gtaacgcgga actccatata tgggctatga actaatgacc
     9181 ccgtaattga ttactattaa taactagtca ataatcaatg tcaacgcatc ga
//
