LOCUS       sequence-449692-        9732 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  GeneGuard genomic cassette: for constructing Delta-thyA::RBS I repA,
            Epsilon, FRT-kanR-FRT (medium-copy support for ColE2 COR). Will 
            support pSEVAT7Z..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9732)
  AUTHORS   Wright O, Delmans M, Stan GB, Ellis T
  TITLE     GeneGuard: a Modular Plasmid System Designed for Biosafety.
  JOURNAL   ACS Synth Biol. 2014 May 13.
  PUBMED    24847673
REFERENCE   2  (bases 1 to 9732)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2014 May 13."
COMMENT     Sequence Label: pSEVA12TEC-I
FEATURES             Location/Qualifiers
     source          1..9732
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(5..682)
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     promoter        1321..1355
                     /label=J23119 promoter
                     /note="bacterial promoter (Registry of Standard Biological 
                     Parts BBa_J23119)"
     terminator      2300..2371
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      2387..2414
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     RBS             2483..2491
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     RBS             2531..2539
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     protein_bind    4172..4205
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     CDS             4573..5367
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4627..4646)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5237..5256
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     protein_bind    5380..5427
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     terminator      5963..6057
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     promoter        6130..6221
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6222..6290,6291..7082)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     terminator      7086..7125
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
     oriT            7272..7380
                     /note="incP origin of transfer"
     CDS             complement(7441..8589)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     IVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     rep_origin      8982..9513
                     /label=oriV
                     /note="incP origin of replication"
     terminator      complement(9640..9726)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ttaattaagc accggtggag tgacgacctt cagcacgttc gtactgttca acgatggtgt
       61 agtcttcgtt gtgggaggtg atgtccagtt tgatgtcggt tttgtaagca cccggcagct
      121 gaaccggttt tttagccatg taggtggttt taacttcagc gtcgtagtga ccaccgtctt
      181 tcagtttcag acgcattttg atttcacctt tcagagcacc gtcttccggg tacatacgtt
      241 cggtggaagc ttcccaaccc atggtttttt tctgcataac cggaccgtcg gacgggaagt
      301 tggtaccacg cagtttaact ttgtagatga actcaccgtc ttgcagggag gagtcctggg
      361 taacggtaac aacaccaccg tcttcgaagt tcataacacg ttcccatttg aaaccttccg
      421 ggaaggacag tttcaggtag tccgggatgt cagccgggtg tttaacgtaa gctttggaac
      481 cgtactggaa ctgcggggac aggatgtccc aagcgaacgg cagcggacca cctttggtaa
      541 ctttcagttt agcggtctgg gtaccttcgt acggacgacc ttcaccttca ccttcgattt
      601 cgaactcgtg accgttaacg gaaccttcca tacgaacttt gaaacgcatg aactctttga
      661 taacgtcttc ggaggaagcc atctagtatt caccaccttt ctctagtaac atttattgta
      721 caacacgagc ccatttttgt caaataaaat ttaaattata tcaacgttaa taagacgttg
      781 tcaataaaat tattttgaca aaattcctag gtctctgatt ttatcgcacc actcattccg
      841 tttggtcttg gtgccgggcg tctgggcaac tttattaacg gtgaattgtg gggccgcgtt
      901 gacccgaact tcccgtttgc catgctgttc cctggctccc gtacagaaga tattttgctg
      961 ctgcaaacca acccgcagtg gcaatccatt ttcgacactt acggtgtgct gccgcgccac
     1021 ccatcacagc tttacgagct gctgctggaa ggtgtggtgc tgtttattat cctcaacctg
     1081 tatattcgta aaccacgccc aatgggagct gtctcaggtt tgttcctgat tggttacggc
     1141 gcgtttcgca tcattgttga gtttttccgc cagcccgacg cgcagtttac cggtgcctgg
     1201 gtgcagtaca tcagcatggg gcaaattctt tccatcccga tgattgtcgc gggtgtgatc
     1261 atgatggtct gggcatatcg tcgcagccca cagcaacacg tttcctgagg aaccgaattc
     1321 ttgacagcta gctcagtcct aggtataatg ctagcggatc tcgtcagtaa cgagccctgg
     1381 gagaaagatg ggtgagcgcc gtacttcaac gcttccgtga aaaattaccg cacaaaccgt
     1441 actgtactaa cgatttcgcg tacggcgttc gcattctgcc gaaaaacatt gccattcttg
     1501 cccgtttcat ccagcagaac cagccacatg cactgtactg gcttcccttt gacgtggacc
     1561 ggacgggggc atcaatcgac tggagcgacc ggaattgtcc ggccccgaac atcaccgtaa
     1621 aaaatccccg taacgggcac gcgcatctgc tctacgcgct cgcccttcct gtgagaactg
     1681 cgccggatgc atcggcttcg gcgctcagat acgctgccgc tattgagcgt gcgttgtgtg
     1741 aaaaactggg cgcggatgtg aattacagcg gcctgatctg caaaaatccg tgccaccctg
     1801 aatggcagga agtggaatgg cgcgaggaac cctacactct cgacgaactg gctgattatc
     1861 tggatctgag cgcctcagcg cgccgtagcg tcgataaaaa ttacgggctg gggcgaaact
     1921 accatctgtt cgaaaaggtc cgtaaatggg cctacagagc gattcgtcag ggctggcctg
     1981 tattctcaca atggcttgat gccgttatcc agcgtgtcga aatgtacaac gcttcgcttc
     2041 ccgttccgct ttctccggct gaatgtcggg ctattggcaa gagcattgcg aaatatacac
     2101 acaggaaatt ctcaccagag ggattttccg ctgtacaggc cgctcgcggt cgcaagggcg
     2161 gaactaaatc taagcgcgca gcagttccta catcagcacg ttcgctgaaa ccgtgggagg
     2221 cattaggcat cagtcgagcg acgtactacc gaaaattaaa atgtgaccca gacctcgcaa
     2281 aataaggatc tccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg
     2341 ttttatctgt tgtttgtcgg tgaacgctct ctactagagt cacactggct caccttcggg
     2401 tgggcctttc tgcgtttata cccgggaaaa agagtattga cttaaagtct aacctatagg
     2461 tataatgtgt ggagaccaga ggtaaggagg taacaaccat gcgagtgttg aagaaacatc
     2521 ttaatcatgc taaggaggtt ttctaatggc agttacgtac tgatgaatcc cctaatgatt
     2581 tttatcaaaa tcattaagtt aaggtagata cacatcttgt catatgatca aatggtttcg
     2641 ccaaaaatca ataatcagac aacaaaatgt gcgaactcga tattttacac gactctcttt
     2701 accaattctg ccccgaatta cacttaaaac gactcaacag cttaacgttg gcttgccacg
     2761 ccttacttga ctgtaaaact ctcactctta ccgaacttgg ccgtaacctg ccaaccaaag
     2821 cgagaacaaa acataacatc aaacgaatcg accgattgtt aggtaatcgt cacctccaca
     2881 aagagcgact cgctgtatac cgttggcatg ctagctttat ctgttcgggc aatacgatgc
     2941 ccattgtact tgttgactgg tctgatatcc gtgagcaaaa acggcttatg gtattgcgag
     3001 cttcagtcgc actacacggt cgttctgtta ctctttatga gaaagcgttc ccgctttcag
     3061 agcaatgttc aaagaaagct catgaccaat ttctagccga ccttgcgagc attctaccga
     3121 gtaacaccac accgctcatt gtcagtgatg ctggctttaa agtgccatgg tataaatccg
     3181 ttgagaagct gggttggtac tggttaagtc gagtaagagg aaaagtacaa tatgcagacc
     3241 taggagcgga aaactggaaa cctatcagca acttacatga tatgtcatct agtcactcaa
     3301 agactttagg ctataagagg ctgactaaaa gcaatccaat ctcatgccaa attctattgt
     3361 ataaatctcg ctctaaaggc cgaaaaaatc agcgctcgac acggactcat tgtcaccacc
     3421 cgtcacctaa aatctactca gcgtcggcaa aggagccatg gattctagca actaacttac
     3481 ctgttgaaat tcgaacaccc aaacaacttg ttaatatcta ttcgaagcga atgcagattg
     3541 aagaaacctt ccgagacttg aaaagtcctg cctacggact aggcctacgc catagccgaa
     3601 cgagcagctc agagcgtttt gatatcatgc tgctaatcgc cctgatgctt caactaacat
     3661 gttggcttgc gggcgttcat gctcagaaac aaggttggga caagcacttc caggctaaca
     3721 cagtcagaaa tcgaaacgta ctctcaacag ttcgcttagg catggaagtt ttgcggcatt
     3781 ctggctacac aataacaagg gaagactcac tcgtggctgc aaccctgctt actcaaaatc
     3841 tattcacaca tggttacgtt ttggggaaat tatgagggga tctctcagag ttacgtatga
     3901 aaaaacattt gaaatagaga tcattaacga attatcggca agcgtttata atcgagtatt
     3961 aaactatgtt ttgaaccatg aattaaataa aaatgactct caattattgg aagtcaattt
     4021 attaaaccaa ttaaagcttg caaaacgtgt aaatcttttt gattattctt tagaagaatt
     4081 acaagccgtt catgagtatt ggcggtcaat gaatcgttac tcaaaacaag ttttgaataa
     4141 agagaaagtg gcttgagtcg acctgcagtt cgaagttcct attctctaga aagtatagga
     4201 acttcagagc gcttttgaag ctcacgctgc cgcaagcact cagggcgcaa gggctgctaa
     4261 aggaagcgga acacgtagaa agccagtccg cagaaacggt gctgaccccg gatgaatgtc
     4321 agctactggg ctatctggac aagggaaaac gcaagcgcaa agagaaagca ggtagcttgc
     4381 agtgggctta catggcgata gctagactgg gcggttttat ggacagcaag cgaaccggaa
     4441 ttgccagctg gggcgccctc tggtaaggtt gggaagccct gcaaagtaaa ctggatggct
     4501 ttcttgccgc caaggatctg atggcgcagg ggatcaagat ctgatcaaga gacaggatga
     4561 ggatcgtttc gcatgattga acaagatgga ttgcacgcag gttctccggc cgcttgggtg
     4621 gagaggctat tcggctatga ctgggcacaa cagacaatcg gctgctctga tgccgccgtg
     4681 ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca agaccgacct gtccggtgcc
     4741 ctgaatgaac tgcaggacga ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct
     4801 tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg actggctgct attgggcgaa
     4861 gtgccggggc aggatctcct gtcatctcac cttgctcctg ccgagaaagt atccatcatg
     4921 gctgatgcaa tgcggcggct gcatacgctt gatccggcta cctgcccatt cgaccaccaa
     4981 gcgaaacatc gcatcgagcg agcacgtact cggatggaag ccggtcttgt cgatcaggat
     5041 gatctggacg aagagcatca ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg
     5101 cgtatgcccg acggcgagga tctcgtcgtg acccatggcg atgcctgctt gccgaatatc
     5161 atggtggaaa atggccgctt ttctggattc atcgactgtg gccggctggg tgtggcggac
     5221 cgctatcagg acatagcgtt ggctacccgt gatattgctg aagagcttgg cggcgaatgg
     5281 gctgaccgct tcctcgtgct ttacggtatc gccgctcccg attcgcagcg catcgccttc
     5341 tatcgccttc ttgacgagtt cttctaataa ggggatcttg aagttcctat tccgaagttc
     5401 ctattctcta gaaagtatag gaacttcgaa gcagctccag cctacagcat gcaaagcgcc
     5461 ggtggctatc taattacgaa acatcctgcc agagccgacg ccagtgtgcg tcggtttttt
     5521 taccctccgt taaattcttc gagacgcctt cccgaaattt tgcaacgtcc tgcaacggcg
     5581 taaatagtcc ggaagatgcg ccgaagaaat agaaactggc cctcgtcttt ttttctcctc
     5641 gctccatact gccggcatga aaacacaacg tggttatacg ctgattgaaa cgctggtcgc
     5701 gatgctgatt ttggtcatgc taagcgcaag tggactctat ggctggcaat actggcagca
     5761 gtcgcaacgg ctatggcaaa ccgccagcca ggcgcgggac tatttgctct atttacgtga
     5821 agatgccaac tggcataacc gcgaccacag tatcagcgtt atcagggagg ggacgttatg
     5881 gtgccttgtg agttccgctg ctggggccaa tacctgtcat ggcagttcac cattggtctt
     5941 tgtgccacgc tgactagtct tggactcctg ttgatagatc cagtaatgac ctcagaactc
     6001 catctggatt tgttcagaac gctcggttgc cgccgggcgt tttttattgg tgagaatcca
     6061 ggggtcccca ataattacga tttaaattag tagcccgcct aatgagcggg ctttttttta
     6121 attcccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat
     6181 aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagcatt cagcattttc
     6241 gtgtggcgct gattccgttt tttgcggcgt tttgcctgcc ggtgtttgcg catccggaaa
     6301 ccctggtgaa agtgaaagat gcggaagatc aactgggtgc gcgcgtgggc tatattgaac
     6361 tggatctgaa cagcggcaaa attctggaat cttttcgtcc ggaagaacgt tttccgatga
     6421 tgagcacctt taaagtgctg ctgtgcggtg cggttctgag ccgtgtggat gcgggccagg
     6481 aacaactggg ccgtcgtatt cattatagcc agaacgatct ggtggaatat agcccggtga
     6541 ccgaaaaaca tctgaccgat ggcatgaccg tgcgtgaact gtgcagcgcg gcgattacca
     6601 tgagcgataa caccgcggcg aacctgctgc tgacgaccat tggcggtccg aaagaactga
     6661 ccgcgtttct gcataacatg ggcgatcatg tgacccgtct ggatcgttgg gaaccggaac
     6721 tgaacgaagc gattccgaac gatgaacgtg ataccaccat gccggcagca atggcgacca
     6781 ccctgcgtaa actgctgacg ggtgagctgc tgaccctggc aagccgccag caactgattg
     6841 attggatgga agcggataaa gtggcgggtc cgctgctgcg tagcgcgctg ccggctggct
     6901 ggtttattgc ggataaaagc ggtgcgggcg aacgtggcag ccgtggcatt attgcggcgc
     6961 tgggcccgga tggtaaaccg agccgtattg tggtgattta taccaccggc agccaggcga
     7021 cgatggatga acgtaaccgt cagattgcgg aaattggcgc gagcctgatt aaacattggt
     7081 aaaccgatac aattaaaggc tccttttgga gccttttttt ttggacgacc cttgtccttt
     7141 tccgctgcat aaccctgctt cggggtcatt atagcgattt tttcggtata tccatccttt
     7201 ttcgcacgat atacaggatt ttgccaaagg gttcgtgtag actttccttg gtgtatccaa
     7261 cggcgtcagc cgggcaggat aggtgaagta ggcccacccg cgagcgggtg ttccttcttc
     7321 actgtccctt attcgcacct ggcggtgctc aacgggaatc ctgctctgcg aggctggccg
     7381 taggccggcc gcgatgcagg tggctgctga acccccagcc ggaactgacc ccacaaggcc
     7441 ctagcgtttg caatgcacca ggtcatcatt gacccaggcg tgttccacca ggccgctgcc
     7501 tcgcaactct tcgcaggctt cgccgacctg ctcgcgccac ttcttcacgc gggtggaatc
     7561 cgatccgcac atgaggcgga aggtttccag cttgagcggg tacggctccc ggtgcgagct
     7621 gaaatagtcg aacatccgtc gggccgtcgg cgacagcttg cggtacttct cccatatgaa
     7681 tttcgtgtag tggtcgccag caaacagcac gacgatttcc tcgtcgatca ggacctggca
     7741 acgggacgtt ttcttgccac ggtccaggac gcggaagcgg tgcagcagcg acaccgattc
     7801 caggtgccca acgcggtcgg acgtgaagcc catcgccgtc gcctgtaggc gcgacaggca
     7861 ttcctcggcc ttcgtgtaat accggccatt gatcgaccag cccaggtcct ggcaaagctc
     7921 gtagaacgtg aaggtgatcg gctcgccgat aggggtgcgc ttcgcgtact ccaacacctg
     7981 ctgccacacc agttcgtcat cgtcggcccg cagctcgacg ccggtgtagg tgatcttcac
     8041 gtccttgttg acgtggaaaa tgaccttgtt ttgcagcgcc tcgcgcggga ttttcttgtt
     8101 gcgcgtggtg aacagggcag agcgggccgt gtcgtttggc atcgctcgca tcgtgtccgg
     8161 ccacggcgca atatcgaaca aggaaagctg catttccttg atctgctgct tcgtgtgttt
     8221 cagcaacgcg gcctgcttgg cttcgctgac ctgttttgcc aggtcctcgc cggcggtttt
     8281 tcgcttcttg gtcgtcatag ttcctcgcgt gtcgatggtc atcgacttcg ccaaacctgc
     8341 cgcctcctgt tcgagacgac gcgaacgctc cacggcggcc gatggcgcgg gcagggcagg
     8401 gggagccagt tgcacgctgt cgcgctcgat cttggccgta gcttgctgga ctatcgagcc
     8461 gacggactgg aaggtttcgc ggggcgcacg catgacggtg cggcttgcga tggtttcggc
     8521 atcctcggcg gaaaaccccg cgtcgatcag ttcttgcctg tatgccttcc ggtcaaacgt
     8581 ccgattcatt caccctcctt gcgggattgc cccggaatta attccccgga tcgatccgtc
     8641 gatcttgatc ccctgcgcca tcagatcctt ggcggcaaga aagccatcca gtttactttg
     8701 cagggcttcc caaccttacc agagggcgcc ccagctggca attccggttc gcttgctgtc
     8761 cataaaaccg cccagtctag ctatcgccat gtaagcccac tgcaagctac ctgctttctc
     8821 tttgcgcttg cgttttccct tgtccagata gcccagtagc tgacattcat ccggggtcag
     8881 caccgtttct gcggactggc tttctacgtg gctgccattt ttggggtgag gccgttcgcg
     8941 gccgaggggc gcagcccctg gggggatggg aggcccgcgt tagcgggccg ggagggttcg
     9001 agaagggggg gcacccccct tcggcgtgcg cggtcacgcg cacagggcgc agccctggtt
     9061 aaaaacaagg tttataaata ttggtttaaa agcaggttaa aagacaggtt agcggtggcc
     9121 gaaaaacggg cggaaaccct tgcaaatgct ggattttctg cctgtggaca gcccctcaaa
     9181 tgtcaatagg tgcgcccctc atctgtcagc actctgcccc tcaagtgtca aggatcgcgc
     9241 ccctcatctg tcagtagtcg cgcccctcaa gtgtcaatac cgcagggcac ttatccccag
     9301 gcttgtccac atcatctgtg ggaaactcgc gtaaaatcag gcgttttcgc cgatttgcga
     9361 ggctggccag ctccacgtcg ccggccgaaa tcgagcctgc ccctcatctg tcaacgccgc
     9421 gccgggtgag tcggcccctc aagtgtcaac gtccgcccct catctgtcag tgagggccaa
     9481 gttttccgcg aggtatccac aacgccggcg gccctacatg gctctgctgt agtgagtggg
     9541 ttgcgctccg gcagcggtcc tgatcccccg cagaaaaaaa ggatctcaag aagatccttt
     9601 gatcttttct acggcgcgcc cagctgtcta gggcggcgga tttgtcctac tcaggagagc
     9661 gttcaccgac aaacaacaga taaaacgaaa ggcccagtct ttcgactgag cctttcgttt
     9721 tatttgatgc ct
//
