LOCUS       sequence-449733-        4833 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  hDLX is an enhancer sequence, designed to drive AAV-mediated 
            transgene expression in cortical GABAergic neurons.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4833)
  AUTHORS   Ben-Simon Y, Hooper M, Narayan S, Daigle TL, Dwivedi D, Way SW, 
            Oster A, Stafford DA, Mich JK, Taormina MJ, Martinez RA, Opitz-Araya
            X, Roth JR, Alexander JR, Allen S, Amster A, Arbuckle J, Ayala A, 
            Baker PM, Bakken TE, Barcelli T, Barta S, Bendrick J, Bertagnolli D,
            Bielstein C, Bishwakarma P, Bowlus J, Boyer G, Brouner K, Casian B, 
            Casper T, Chakka AB, Chakrabarty R, Chance RK, Chavan S, Clark M, 
            Colbert K, Collman F, Daniel S, Departee M, DiValentin P, Donadio N,
            Dotson N, Egdorf T, Fliss T, Gabitto M, Garcia J, Gary A, Gasperini 
            M, Gloe J, Goldy J, Gore BB, Graybuck L, Greisman N, Haeseleer F, 
            Halterman C, Haradon Z, Hastings SD, Helback O, Ho W, Hockemeyer D, 
            Huang C, Huff S, Hunker A, Johansen N, Jones D, Juneau Z, Kalmbach 
            B, Kannan M, Khem S, Kussick E, Kutsal R, Larsen R, Lee C, Lee AY, 
            Leibly M, Lenz GH, Li S, Liang E, Lusk N, Madigan Z, Malloy J, 
            Malone J, McCue R, Melchor J, Mollenkopf T, Moosman S, Morin E, 
            Newman D, Ng L, Ngo K, Omstead V, Otto S, Oyama A, Pena N, Pham T, 
            Phillips E, Pom CA, Potekhina L, Ransford S, Ray PL, Rette D, 
            Reynoldson C, Rimorin C, Rocha D, Ruiz A, Sanchez REA, Sawyer L, 
            Sedeno-Cortes A, Sevigny JP, Shapovalova N, Shepard N, Shulga L, 
            Sigler AR, Siverts L, Soliman S, Somasundaram S, Staats B, Stewart 
            K, Szelenyi E, Tieu M, Trader C, Tran A, van Velthoven CTJ, Walker 
            M, Wang Y, Weed N, Wirthlin M, Wood T, Wynalda B, Yao Z, Zhou T, 
            Ariza J, Dee N, Reding M, Ronellenfitch K, Mufti S, Sunkin SM, Smith
            KA, Esposito L, Waters J, Thyagarajan B, Yao S, Lein ES, Zeng H, 
            Levi BP, Ngai J, Ting JT, Tasic B
  TITLE     A suite of enhancer AAVs and transgenic mouse lines for genetic 
            access to cortical cell types.
  JOURNAL   Cell. 2025 May 16:S0092-8674(25)00513-6. doi: 
            10.1016/j.cell.2025.05.002.
  PUBMED    40403729
REFERENCE   2  (bases 1 to 4833)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2025 
            May 16:S0092-8674(25)00513-6. doi: 10.1016/j.cell.2025.05.002."
COMMENT     Sequence Label: AiP13084 - pAAV-hDLX-minBG-iCre(R297T)-BGHpA (...
FEATURES             Location/Qualifiers
     source          1..4833
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     regulatory      769..778
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             781..801
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    1867..1978
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   2096..2236
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   2096..2225
                     /label=AAV2 ITR
     rep_origin      2311..2766
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2398..2417)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2608..2629
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(2783..2802)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2902..2924
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(2962..2980)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3048..3152
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3153..3221,3222..4013)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3371..3390)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4184..4772
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4673..4692
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtataggt accgagctct atgcactcac agtggtttgg
      181 catgcatctg gtgaattttt tttaacgaaa aattagtgtt ggtttcgatg tatggtagca
      241 ttctccctaa cgtaatttga ataattcagc aaagccccac taccagctgt acttctgcag
      301 cctcttccat tcttttcagc attataattt tggttaattt tcaattttag gtcctacgtc
      361 tctgcaattt gtgtatgaat aacagaataa tttccctctt ttgtttcgcc tttcctgttc
      421 ctgaatctaa ataaagatgg ctttttagta ttaaaagtgg aagaaaatta caggtaatta
      481 tctttgacgg taaaaacgct gtaatcagcg ggctacatga aaaattactc taattatggc
      541 tgcatttaag agaatggaaa aaaaccttct tgtggataaa aaccttaaat tgtccccaat
      601 gtctgcttca aattggatgg cactgcagct ggaggctttg ttcagaattg atcctgggga
      661 gctacgaacc caaagtttca cagtagggag ctcgggctgg gcataaaagt cagggcagag
      721 ccatctattg cttacatttg cttctgggat ccagatcttt cgaagctagc caccatggtg
      781 cccaagaaga agaggaaagt ctccaacctg ctgactgtgc accaaaacct gcctgccctc
      841 cctgtggatg ccacctctga tgaagtcagg aagaacctga tggacatgtt cagggacagg
      901 caggccttct ctgaacacac ctggaagatg ctcctgtctg tgtgcagatc ctgggctgcc
      961 tggtgcaagc tgaacaacag gaaatggttc cctgctgaac ctgaggatgt gagggactac
     1021 ctcctgtacc tgcaagccag aggcctggct gtgaagacca tccaacagca cctgggccag
     1081 ctcaacatgc tgcacaggag atctggcctg cctcgccctt ctgactccaa tgctgtgtcc
     1141 ctggtgatga ggagaatcag aaaggagaat gtggatgctg gggagagagc caagcaggcc
     1201 ctggcctttg aacgcactga ctttgaccaa gtcagatccc tgatggagaa ctctgacaga
     1261 tgccaggaca tcaggaacct ggccttcctg ggcattgcct acaacaccct gctgcgcatt
     1321 gccgaaattg ccagaatcag agtgaaggac atctcccgca ccgatggtgg gagaatgctg
     1381 atccacattg gcaggaccaa gaccctggtg tccacagctg gtgtggagaa ggccctgtcc
     1441 ctgggggtta ccaagctggt ggagagatgg atctctgtgt ctggtgtggc tgatgacccc
     1501 aacaactacc tgttctgccg ggtcagaaag aatggtgtgg ctgccccttc tgccacctcc
     1561 caactgtcca cccgggccct ggaagggatc tttgaggcca cccaccgcct gatctatggt
     1621 gccaaggatg actctgggca gagatacctg gcctggtctg gccactctgc cagagtgggt
     1681 gctgccaccg acatggccag ggctggtgtg tccatccctg aaatcatgca ggctggtggc
     1741 tggaccaatg tgaacattgt gatgaactac atcagaaacc tggactctga gactggggcc
     1801 atggtgaggc tgctcgaaga tggggactga ggcgcgccga attcaagctt ctcgagagat
     1861 cttcgactgt gccttctagt tgccagccat ctgttgtttg cccctccccc gtgccttcct
     1921 tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa attgcatcgc
     1981 attgtctgag taggtgtcat tctattctgg ggggtggggt ggggcaggac agcaaggggg
     2041 aggattggga agacaatagc aggcatgaga tctcacgtgc ggaccgagcg gccgcaggaa
     2101 cccctagtga tggagttggc cactccctct ctgcgcgctc gctcgctcac tgaggccggg
     2161 cgaccaaagg tcgcccgacg cccgggcttt gcccgggcgg cctcagtgag cgagcgagcg
     2221 cgcagctgcc tgcaggggcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt
     2281 tcacaccgca tacgtcaaag caaccatagt acgcgccctg tagcggcgca ttaagcgcgg
     2341 cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcctta gcgcccgctc
     2401 ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa
     2461 atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac
     2521 ttgatttggg tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt
     2581 tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca
     2641 actctatctc gggctattct tttgatttat aagggatttt gccgatttcg gtctattggt
     2701 taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgttta
     2761 caattttatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagcccc
     2821 gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt
     2881 acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac
     2941 cgaaacgcgc gagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga
     3001 taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta
     3061 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat
     3121 aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc
     3181 ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga
     3241 aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca
     3301 acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt
     3361 ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg
     3421 gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc
     3481 atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata
     3541 acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt
     3601 tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag
     3661 ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca
     3721 aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg
     3781 aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg
     3841 ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag
     3901 atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg
     3961 aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag
     4021 accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga
     4081 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt
     4141 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
     4201 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
     4261 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
     4321 caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
     4381 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
     4441 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
     4501 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
     4561 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
     4621 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
     4681 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
     4741 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
     4801 tcctggcctt ttgctggcct tttgctcaca tgt
//
