LOCUS       sequence-44979-e         903 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 903)
  AUTHORS   Zhao Y, Araki S, Wu J, Teramoto T, Chang YF, Nakano M, Abdelfattah 
            AS, Fujiwara M, Ishihara T, Nagai T, Campbell RE
  TITLE     An Expanded Palette of Genetically Encoded Ca2+ Indicators.
  JOURNAL   Science. 2011 Sep 30;333(6051):1888-91. doi: 10.1126/science.1208592
  PUBMED    21903779
REFERENCE   2  (bases 1 to 903)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2011-09-30- 30"; volume: "333"; 
            issue: "6051"; pages: "1888-91"
COMMENT     Sequence Label: pTorPE-GEX-GECO1
FEATURES             Location/Qualifiers
     source          1..903
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(9..452)
                     /codon_start=1
                     /product="calcium-binding protein"
                     /label=calmodulin
                     /translation="DQLTEEQIAELKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEA
                     ELQDMINEVDADGDGTIDLPEFQTMMARKMNDTDSEEEIREAFRVFDKDGNGYIGAAEL
                     RHVMTNLGEKLTDEEVDEMIRVADIDGDGQVNYEEFVQMMTAK"
     primer_bind     568..587
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 gccagcttct acttcgctgt catcatttgt acaaactctt cgtagtttac ctgaccatcc
       61 ccatcgatgt ctgctaccct gatcatttca tctacctctt catctgttaa cttctctcca
      121 aggtttgtca tcacgtggcg aagctctgct gcgccgatgt agccattgcc gtccttatca
      181 aacacacgga acgcttctct aatttcctct tcactgtctg tgtcattcat ttttcttgcc
      241 atcattgtct ggaactcagg gaggtcgatt gtgccgtcac cgtcggcatc tacttcattg
      301 atcatgtcct gcagctctgc ttctgtgggg ttctgcccca gagaccgcat caccgtcccc
      361 agctccttgg ttgttatcgt cccatccccg tccttgtcaa atagggagaa agcctcttta
      421 agttctgcga tctgctcttc agtcagttgg tcacgcgtgt tgtactccag cttgtgcccc
      481 aggatgttgc cgtcctcctt gaagtcgatg cccttcagct cgatgcggtt caccagggtg
      541 tcgccctcga acttcacctc ggcgcgggtc ttgtagttgc cgtcgtcctt gaagaagatg
      601 gtgcgctcct ggatgtagcc ttcgggcatg gcggacttga agaagtcgtg ctgcttcatg
      661 tggtcggggt agcggctgaa gcactgcacg ccataggaca gggtggtcac gagggtgggc
      721 cagggcacgg gcagcttgcc ggtggtgcag atgaacttca gggtcagctt gccgtaggtg
      781 gcatcgccct caccctcgcc ggacacgctg aacttgtggc cgtttacgtc gccgtccagc
      841 tcgacctgga tgggcaccac cccggtgaac agctcctcgc ccttgctcac catgctccct
      901 ccg
//
